The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Nancy L Wengenack - One of the best experts on this subject based on the ideXlab platform.
-
deactivation of the dimorphic fungi histoplasma capsulatum blastomyces dermatitidis and coccidioides immitis using hydrogen peroxide vapor
Medical Mycology, 2008Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Hydrogen peroxide vapour (HPV) has been proposed as an alternative to formaldehyde fumigation for the decontamination of bioSafety level (BSL) III laboratories. The aim of this study was to evaluate the efficacy of HPV against the dimorphic fungi Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis. Working inside a class II Biological Safety Cabinet (BSC) within a BSL III laboratory, inocula containing approximately 5-log10 cfu/ml from the mould form of each organism suspended in RPMI medium were deposited on stainless steel discs and allowed to air dry. The organisms were exposed to HPV inside a BSC using a BIOQUELL ClarusS HPV generator. In three replicate experiments, individual discs were transferred into liquid media at timed intervals during a 105 minute HPV exposure period. Control- and HPV-exposed discs were incubated in RPMI media at 30°C for 6 weeks to determine if any viable organisms remained. Positive cultures were confirmed using specific nucleic acid hybridization prob...
-
deactivation of the dimorphic fungi histoplasma capsulatum blastomyces dermatitidis and coccidioides immitis using hydrogen peroxide vapor
Medical Mycology, 2008Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Hydrogen peroxide vapour (HPV) has been proposed as an alternative to formaldehyde fumigation for the decontamination of bioSafety level (BSL) III laboratories. The aim of this study was to evaluate the efficacy of HPV against the dimorphic fungi Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis. Working inside a class II Biological Safety Cabinet (BSC) within a BSL III laboratory, inocula containing approximately 5-log(10) cfu/ml from the mould form of each organism suspended in RPMI medium were deposited on stainless steel discs and allowed to air dry. The organisms were exposed to HPV inside a BSC using a BIOQUELL ClarusS HPV generator. In three replicate experiments, individual discs were transferred into liquid media at timed intervals during a 105 minute HPV exposure period. Control- and HPV-exposed discs were incubated in RPMI media at 30 degrees C for 6 weeks to determine if any viable organisms remained. Positive cultures were confirmed using specific nucleic acid hybridization probes. Results indicate that H. capsulatum, B. dermatitidis and C. immitis were killed within 30 minutes of HPV exposure.
-
use of hydrogen peroxide vapor for deactivation of mycobacterium tuberculosis in a Biological Safety Cabinet and a room
Journal of Clinical Microbiology, 2007Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Mycobacterium tuberculosis is an important human pathogen that is routinely cultured in clinical and research laboratories. M. tuberculosis can contaminate surfaces and is highly resistant to disinfection. We investigated whether hydrogen peroxide vapor (HPV) is effective for the deactivation of M. tuberculosis on experimentally contaminated surfaces in a Biological Safety Cabinet (BSC) and a room. Biological indicators (BIs) consisting of an ∼3-log10 inoculum of M. tuberculosis on stainless steel discs and a 6-log10 inoculum of Geobacillus stearothermophilus were exposed to HPV in BSC time course experiments and at 10 locations during room experiments. In three separate BSC experiments, M. tuberculosis BIs were transferred to growth media at 15-min intervals during a 180-min HPV exposure period. No M. tuberculosis BIs grew following 30 min of HPV exposure. In three separate room experiments, M. tuberculosis and G. stearothermophilus BIs were exposed to HPV for 90, 120, and 150 min, respectively. BIs for both microorganisms were deactivated in all 10 locations following 90 min of HPV exposure. HPV provides an alternative to traditional decontamination methods, such as formaldehyde fumigation, for laboratories and other areas contaminated with M. tuberculosis.
Leslie Hall - One of the best experts on this subject based on the ideXlab platform.
-
deactivation of the dimorphic fungi histoplasma capsulatum blastomyces dermatitidis and coccidioides immitis using hydrogen peroxide vapor
Medical Mycology, 2008Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Hydrogen peroxide vapour (HPV) has been proposed as an alternative to formaldehyde fumigation for the decontamination of bioSafety level (BSL) III laboratories. The aim of this study was to evaluate the efficacy of HPV against the dimorphic fungi Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis. Working inside a class II Biological Safety Cabinet (BSC) within a BSL III laboratory, inocula containing approximately 5-log10 cfu/ml from the mould form of each organism suspended in RPMI medium were deposited on stainless steel discs and allowed to air dry. The organisms were exposed to HPV inside a BSC using a BIOQUELL ClarusS HPV generator. In three replicate experiments, individual discs were transferred into liquid media at timed intervals during a 105 minute HPV exposure period. Control- and HPV-exposed discs were incubated in RPMI media at 30°C for 6 weeks to determine if any viable organisms remained. Positive cultures were confirmed using specific nucleic acid hybridization prob...
-
deactivation of the dimorphic fungi histoplasma capsulatum blastomyces dermatitidis and coccidioides immitis using hydrogen peroxide vapor
Medical Mycology, 2008Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Hydrogen peroxide vapour (HPV) has been proposed as an alternative to formaldehyde fumigation for the decontamination of bioSafety level (BSL) III laboratories. The aim of this study was to evaluate the efficacy of HPV against the dimorphic fungi Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis. Working inside a class II Biological Safety Cabinet (BSC) within a BSL III laboratory, inocula containing approximately 5-log(10) cfu/ml from the mould form of each organism suspended in RPMI medium were deposited on stainless steel discs and allowed to air dry. The organisms were exposed to HPV inside a BSC using a BIOQUELL ClarusS HPV generator. In three replicate experiments, individual discs were transferred into liquid media at timed intervals during a 105 minute HPV exposure period. Control- and HPV-exposed discs were incubated in RPMI media at 30 degrees C for 6 weeks to determine if any viable organisms remained. Positive cultures were confirmed using specific nucleic acid hybridization probes. Results indicate that H. capsulatum, B. dermatitidis and C. immitis were killed within 30 minutes of HPV exposure.
-
use of hydrogen peroxide vapor for deactivation of mycobacterium tuberculosis in a Biological Safety Cabinet and a room
Journal of Clinical Microbiology, 2007Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Mycobacterium tuberculosis is an important human pathogen that is routinely cultured in clinical and research laboratories. M. tuberculosis can contaminate surfaces and is highly resistant to disinfection. We investigated whether hydrogen peroxide vapor (HPV) is effective for the deactivation of M. tuberculosis on experimentally contaminated surfaces in a Biological Safety Cabinet (BSC) and a room. Biological indicators (BIs) consisting of an ∼3-log10 inoculum of M. tuberculosis on stainless steel discs and a 6-log10 inoculum of Geobacillus stearothermophilus were exposed to HPV in BSC time course experiments and at 10 locations during room experiments. In three separate BSC experiments, M. tuberculosis BIs were transferred to growth media at 15-min intervals during a 180-min HPV exposure period. No M. tuberculosis BIs grew following 30 min of HPV exposure. In three separate room experiments, M. tuberculosis and G. stearothermophilus BIs were exposed to HPV for 90, 120, and 150 min, respectively. BIs for both microorganisms were deactivated in all 10 locations following 90 min of HPV exposure. HPV provides an alternative to traditional decontamination methods, such as formaldehyde fumigation, for laboratories and other areas contaminated with M. tuberculosis.
Stuart D Blacksell - One of the best experts on this subject based on the ideXlab platform.
-
laboratory acquired scrub typhus and murine typhus infections the argument for a risk based approach to bioSafety requirements for orientia tsutsugamushi and rickettsia typhi laboratory activities
Clinical Infectious Diseases, 2019Co-Authors: Stuart D Blacksell, Matthew T Robinson, Paul N Newton, Nicholas P J DayAbstract:This study examined the literature on laboratory-acquired infections (LAIs) associated with scrub typhus (Orientia tsutsugamushi) and murine typhus (Rickettsia typhi) research to provide an evidence base for bioSafety and biocontainment. Scrub typhus LAIs were documented in 25 individuals, from 1931 to 2000 with 8 (32%) deaths during the preantibiotic era. There were 35 murine typhus LAI reports and no deaths. Results indicated that the highest-risk activities were working with infectious laboratory animals involving significant aerosol exposures, accidental self-inoculation, or bite-related infections. A risk-based bioSafety approach for in vitro and in vivo culture of O. tsutsugamushi and R. typhi would require that only high-risk activities (animal work or large culture volumes) be performed in high-containment bioSafety level (BSL) 3 laboratories. We argue that relatively low-risk activities including inoculation of cell cultures or the early stages of in vitro growth using low volumes/low concentrations of infectious materials can be performed safely in BSL-2 laboratories within a Biological Safety Cabinet.
-
a Biological Safety Cabinet certification program experiences in southeast asia
Applied Biosafety, 2016Co-Authors: Toni Whistler, Anek Kaewpan, Stuart D BlacksellAbstract:Biological Safety Cabinets (BSCs) are the primary means of containment used in laboratories worldwide for the safe handling of infectious microorganisms. They provide protection to the laboratory w...
Jonathan A Otter - One of the best experts on this subject based on the ideXlab platform.
-
deactivation of the dimorphic fungi histoplasma capsulatum blastomyces dermatitidis and coccidioides immitis using hydrogen peroxide vapor
Medical Mycology, 2008Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Hydrogen peroxide vapour (HPV) has been proposed as an alternative to formaldehyde fumigation for the decontamination of bioSafety level (BSL) III laboratories. The aim of this study was to evaluate the efficacy of HPV against the dimorphic fungi Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis. Working inside a class II Biological Safety Cabinet (BSC) within a BSL III laboratory, inocula containing approximately 5-log10 cfu/ml from the mould form of each organism suspended in RPMI medium were deposited on stainless steel discs and allowed to air dry. The organisms were exposed to HPV inside a BSC using a BIOQUELL ClarusS HPV generator. In three replicate experiments, individual discs were transferred into liquid media at timed intervals during a 105 minute HPV exposure period. Control- and HPV-exposed discs were incubated in RPMI media at 30°C for 6 weeks to determine if any viable organisms remained. Positive cultures were confirmed using specific nucleic acid hybridization prob...
-
deactivation of the dimorphic fungi histoplasma capsulatum blastomyces dermatitidis and coccidioides immitis using hydrogen peroxide vapor
Medical Mycology, 2008Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Hydrogen peroxide vapour (HPV) has been proposed as an alternative to formaldehyde fumigation for the decontamination of bioSafety level (BSL) III laboratories. The aim of this study was to evaluate the efficacy of HPV against the dimorphic fungi Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis. Working inside a class II Biological Safety Cabinet (BSC) within a BSL III laboratory, inocula containing approximately 5-log(10) cfu/ml from the mould form of each organism suspended in RPMI medium were deposited on stainless steel discs and allowed to air dry. The organisms were exposed to HPV inside a BSC using a BIOQUELL ClarusS HPV generator. In three replicate experiments, individual discs were transferred into liquid media at timed intervals during a 105 minute HPV exposure period. Control- and HPV-exposed discs were incubated in RPMI media at 30 degrees C for 6 weeks to determine if any viable organisms remained. Positive cultures were confirmed using specific nucleic acid hybridization probes. Results indicate that H. capsulatum, B. dermatitidis and C. immitis were killed within 30 minutes of HPV exposure.
-
use of hydrogen peroxide vapor for deactivation of mycobacterium tuberculosis in a Biological Safety Cabinet and a room
Journal of Clinical Microbiology, 2007Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Mycobacterium tuberculosis is an important human pathogen that is routinely cultured in clinical and research laboratories. M. tuberculosis can contaminate surfaces and is highly resistant to disinfection. We investigated whether hydrogen peroxide vapor (HPV) is effective for the deactivation of M. tuberculosis on experimentally contaminated surfaces in a Biological Safety Cabinet (BSC) and a room. Biological indicators (BIs) consisting of an ∼3-log10 inoculum of M. tuberculosis on stainless steel discs and a 6-log10 inoculum of Geobacillus stearothermophilus were exposed to HPV in BSC time course experiments and at 10 locations during room experiments. In three separate BSC experiments, M. tuberculosis BIs were transferred to growth media at 15-min intervals during a 180-min HPV exposure period. No M. tuberculosis BIs grew following 30 min of HPV exposure. In three separate room experiments, M. tuberculosis and G. stearothermophilus BIs were exposed to HPV for 90, 120, and 150 min, respectively. BIs for both microorganisms were deactivated in all 10 locations following 90 min of HPV exposure. HPV provides an alternative to traditional decontamination methods, such as formaldehyde fumigation, for laboratories and other areas contaminated with M. tuberculosis.
John Chewins - One of the best experts on this subject based on the ideXlab platform.
-
deactivation of the dimorphic fungi histoplasma capsulatum blastomyces dermatitidis and coccidioides immitis using hydrogen peroxide vapor
Medical Mycology, 2008Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Hydrogen peroxide vapour (HPV) has been proposed as an alternative to formaldehyde fumigation for the decontamination of bioSafety level (BSL) III laboratories. The aim of this study was to evaluate the efficacy of HPV against the dimorphic fungi Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis. Working inside a class II Biological Safety Cabinet (BSC) within a BSL III laboratory, inocula containing approximately 5-log10 cfu/ml from the mould form of each organism suspended in RPMI medium were deposited on stainless steel discs and allowed to air dry. The organisms were exposed to HPV inside a BSC using a BIOQUELL ClarusS HPV generator. In three replicate experiments, individual discs were transferred into liquid media at timed intervals during a 105 minute HPV exposure period. Control- and HPV-exposed discs were incubated in RPMI media at 30°C for 6 weeks to determine if any viable organisms remained. Positive cultures were confirmed using specific nucleic acid hybridization prob...
-
deactivation of the dimorphic fungi histoplasma capsulatum blastomyces dermatitidis and coccidioides immitis using hydrogen peroxide vapor
Medical Mycology, 2008Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Hydrogen peroxide vapour (HPV) has been proposed as an alternative to formaldehyde fumigation for the decontamination of bioSafety level (BSL) III laboratories. The aim of this study was to evaluate the efficacy of HPV against the dimorphic fungi Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis. Working inside a class II Biological Safety Cabinet (BSC) within a BSL III laboratory, inocula containing approximately 5-log(10) cfu/ml from the mould form of each organism suspended in RPMI medium were deposited on stainless steel discs and allowed to air dry. The organisms were exposed to HPV inside a BSC using a BIOQUELL ClarusS HPV generator. In three replicate experiments, individual discs were transferred into liquid media at timed intervals during a 105 minute HPV exposure period. Control- and HPV-exposed discs were incubated in RPMI media at 30 degrees C for 6 weeks to determine if any viable organisms remained. Positive cultures were confirmed using specific nucleic acid hybridization probes. Results indicate that H. capsulatum, B. dermatitidis and C. immitis were killed within 30 minutes of HPV exposure.
-
use of hydrogen peroxide vapor for deactivation of mycobacterium tuberculosis in a Biological Safety Cabinet and a room
Journal of Clinical Microbiology, 2007Co-Authors: Leslie Hall, Jonathan A Otter, John Chewins, Nancy L WengenackAbstract:Mycobacterium tuberculosis is an important human pathogen that is routinely cultured in clinical and research laboratories. M. tuberculosis can contaminate surfaces and is highly resistant to disinfection. We investigated whether hydrogen peroxide vapor (HPV) is effective for the deactivation of M. tuberculosis on experimentally contaminated surfaces in a Biological Safety Cabinet (BSC) and a room. Biological indicators (BIs) consisting of an ∼3-log10 inoculum of M. tuberculosis on stainless steel discs and a 6-log10 inoculum of Geobacillus stearothermophilus were exposed to HPV in BSC time course experiments and at 10 locations during room experiments. In three separate BSC experiments, M. tuberculosis BIs were transferred to growth media at 15-min intervals during a 180-min HPV exposure period. No M. tuberculosis BIs grew following 30 min of HPV exposure. In three separate room experiments, M. tuberculosis and G. stearothermophilus BIs were exposed to HPV for 90, 120, and 150 min, respectively. BIs for both microorganisms were deactivated in all 10 locations following 90 min of HPV exposure. HPV provides an alternative to traditional decontamination methods, such as formaldehyde fumigation, for laboratories and other areas contaminated with M. tuberculosis.