The Experts below are selected from a list of 132 Experts worldwide ranked by ideXlab platform

Chongyang Liang - One of the best experts on this subject based on the ideXlab platform.

  • in situ accurate surface enhanced raman scattering detection of cancer cell nucleus with synchronous location by an alkyne labeled Biomolecular Probe
    Analytical and Bioanalytical Chemistry, 2018
    Co-Authors: Jing Zhang, Lijia Liang, Xin Guan, Rong Deng, Dianshuai Huang, Chongyang Liang
    Abstract:

    A surface-enhanced Raman scattering (SERS) method for in situ detection and analysis of the intranuclear Biomolecular information of a cell has been developed based on a small, biocompatible, nuclear-targeting alkyne-tagged deoxyribonucleic acid (DNA) Probe (5-ethynyl-2'-deoxyuridine, EDU) that can specially accumulate in the cell nucleus during DNA replications to precisely locate the nuclear region without disturbance in cell biological activities and functions. Since the specific alkyne group shows a Raman peak in the Raman-silent region of cells, it is an interior label to visualize the nuclear location synchronously in real time when measuring the SERS spectra of a cell. Because no fluorescent-labeled dyes were used for locating cell nuclei, this method is simple, nondestructive, non- photobleaching, and valuable for the in situ exploration of vital physiological processes with DNA participation in cell organelles. Graphical abstract A universal strategy was developed to accurately locate the nuclear region and obtain precise molecular information of cell nuclei by SERS.

  • In situ, accurate, surface-enhanced Raman scattering detection of cancer cell nucleus with synchronous location by an alkyne-labeled Biomolecular Probe.
    Analytical and bioanalytical chemistry, 2017
    Co-Authors: Jing Zhang, Lijia Liang, Xin Guan, Rong Deng, Dianshuai Huang, Chongyang Liang
    Abstract:

    A surface-enhanced Raman scattering (SERS) method for in situ detection and analysis of the intranuclear Biomolecular information of a cell has been developed based on a small, biocompatible, nuclear-targeting alkyne-tagged deoxyribonucleic acid (DNA) Probe (5-ethynyl-2′-deoxyuridine, EDU) that can specially accumulate in the cell nucleus during DNA replications to precisely locate the nuclear region without disturbance in cell biological activities and functions. Since the specific alkyne group shows a Raman peak in the Raman-silent region of cells, it is an interior label to visualize the nuclear location synchronously in real time when measuring the SERS spectra of a cell. Because no fluorescent-labeled dyes were used for locating cell nuclei, this method is simple, nondestructive, non- photobleaching, and valuable for the in situ exploration of vital physiological processes with DNA participation in cell organelles.

Jing Zhang - One of the best experts on this subject based on the ideXlab platform.

  • in situ accurate surface enhanced raman scattering detection of cancer cell nucleus with synchronous location by an alkyne labeled Biomolecular Probe
    Analytical and Bioanalytical Chemistry, 2018
    Co-Authors: Jing Zhang, Lijia Liang, Xin Guan, Rong Deng, Dianshuai Huang, Chongyang Liang
    Abstract:

    A surface-enhanced Raman scattering (SERS) method for in situ detection and analysis of the intranuclear Biomolecular information of a cell has been developed based on a small, biocompatible, nuclear-targeting alkyne-tagged deoxyribonucleic acid (DNA) Probe (5-ethynyl-2'-deoxyuridine, EDU) that can specially accumulate in the cell nucleus during DNA replications to precisely locate the nuclear region without disturbance in cell biological activities and functions. Since the specific alkyne group shows a Raman peak in the Raman-silent region of cells, it is an interior label to visualize the nuclear location synchronously in real time when measuring the SERS spectra of a cell. Because no fluorescent-labeled dyes were used for locating cell nuclei, this method is simple, nondestructive, non- photobleaching, and valuable for the in situ exploration of vital physiological processes with DNA participation in cell organelles. Graphical abstract A universal strategy was developed to accurately locate the nuclear region and obtain precise molecular information of cell nuclei by SERS.

  • In situ, accurate, surface-enhanced Raman scattering detection of cancer cell nucleus with synchronous location by an alkyne-labeled Biomolecular Probe.
    Analytical and bioanalytical chemistry, 2017
    Co-Authors: Jing Zhang, Lijia Liang, Xin Guan, Rong Deng, Dianshuai Huang, Chongyang Liang
    Abstract:

    A surface-enhanced Raman scattering (SERS) method for in situ detection and analysis of the intranuclear Biomolecular information of a cell has been developed based on a small, biocompatible, nuclear-targeting alkyne-tagged deoxyribonucleic acid (DNA) Probe (5-ethynyl-2′-deoxyuridine, EDU) that can specially accumulate in the cell nucleus during DNA replications to precisely locate the nuclear region without disturbance in cell biological activities and functions. Since the specific alkyne group shows a Raman peak in the Raman-silent region of cells, it is an interior label to visualize the nuclear location synchronously in real time when measuring the SERS spectra of a cell. Because no fluorescent-labeled dyes were used for locating cell nuclei, this method is simple, nondestructive, non- photobleaching, and valuable for the in situ exploration of vital physiological processes with DNA participation in cell organelles.

Veronika Novakova - One of the best experts on this subject based on the ideXlab platform.

  • synthesis and j dimer formation of tetrapyrazinoporphyrazines with different functional groups for potential Biomolecular Probe applications
    ChemPlusChem, 2020
    Co-Authors: Jiri Demuth, Miroslav Miletin, Matej Machan, Michal Kantor, Petr Zimcik, Veronika Novakova
    Abstract:

    Though tetrapyrazinoporphyrazines (TPyzPzs) are generally presented as universal dark quenchers for oligonucleotide Probes, the availability of TPyzPzs bearing different functional groups suitable for attachment to 3', and 5' ends or intrastrand positions remains rather limited. Therefore, a synthetic route to hexa(bis(2-methoxyethyl)amino) or hexa(diethylamino) TPyzPzs functionalized by an azide, hydroxy, or carboxy group or their combinations was developed. Studies of self-assembly into J-dimers in nonpolar solvents and their stability upon titration with pyridine (association constants, KP values, ranging 0.32-12.7×102  M-1 ) revealed that smaller peripheral substituents and functionalization of TPyzPzs improves the stability of J-dimers. ΦΔ and ΦF were low for the monomers (ΦF <0.0001, ΦΔ <0.008, DMF) due to quenching by intramolecular charge transfer; however, they increased in nonpolar solvents and after self-assembly into J-dimer (up to ΦF =0.027, ΦΔ =0.28).

  • Synthesis and J‐Dimer Formation of Tetrapyrazinoporphyrazines with Different Functional Groups for Potential Biomolecular Probe Applications
    ChemPlusChem, 2020
    Co-Authors: Jiri Demuth, Miroslav Miletin, Matej Machan, Michal Kantor, Petr Zimcik, Veronika Novakova
    Abstract:

    Though tetrapyrazinoporphyrazines (TPyzPzs) are generally presented as universal dark quenchers for oligonucleotide Probes, the availability of TPyzPzs bearing different functional groups suitable for attachment to 3', and 5' ends or intrastrand positions remains rather limited. Therefore, a synthetic route to hexa(bis(2-methoxyethyl)amino) or hexa(diethylamino) TPyzPzs functionalized by an azide, hydroxy, or carboxy group or their combinations was developed. Studies of self-assembly into J-dimers in nonpolar solvents and their stability upon titration with pyridine (association constants, KP values, ranging 0.32-12.7×102  M-1 ) revealed that smaller peripheral substituents and functionalization of TPyzPzs improves the stability of J-dimers. ΦΔ and ΦF were low for the monomers (ΦF

Dianshuai Huang - One of the best experts on this subject based on the ideXlab platform.

  • in situ accurate surface enhanced raman scattering detection of cancer cell nucleus with synchronous location by an alkyne labeled Biomolecular Probe
    Analytical and Bioanalytical Chemistry, 2018
    Co-Authors: Jing Zhang, Lijia Liang, Xin Guan, Rong Deng, Dianshuai Huang, Chongyang Liang
    Abstract:

    A surface-enhanced Raman scattering (SERS) method for in situ detection and analysis of the intranuclear Biomolecular information of a cell has been developed based on a small, biocompatible, nuclear-targeting alkyne-tagged deoxyribonucleic acid (DNA) Probe (5-ethynyl-2'-deoxyuridine, EDU) that can specially accumulate in the cell nucleus during DNA replications to precisely locate the nuclear region without disturbance in cell biological activities and functions. Since the specific alkyne group shows a Raman peak in the Raman-silent region of cells, it is an interior label to visualize the nuclear location synchronously in real time when measuring the SERS spectra of a cell. Because no fluorescent-labeled dyes were used for locating cell nuclei, this method is simple, nondestructive, non- photobleaching, and valuable for the in situ exploration of vital physiological processes with DNA participation in cell organelles. Graphical abstract A universal strategy was developed to accurately locate the nuclear region and obtain precise molecular information of cell nuclei by SERS.

  • In situ, accurate, surface-enhanced Raman scattering detection of cancer cell nucleus with synchronous location by an alkyne-labeled Biomolecular Probe.
    Analytical and bioanalytical chemistry, 2017
    Co-Authors: Jing Zhang, Lijia Liang, Xin Guan, Rong Deng, Dianshuai Huang, Chongyang Liang
    Abstract:

    A surface-enhanced Raman scattering (SERS) method for in situ detection and analysis of the intranuclear Biomolecular information of a cell has been developed based on a small, biocompatible, nuclear-targeting alkyne-tagged deoxyribonucleic acid (DNA) Probe (5-ethynyl-2′-deoxyuridine, EDU) that can specially accumulate in the cell nucleus during DNA replications to precisely locate the nuclear region without disturbance in cell biological activities and functions. Since the specific alkyne group shows a Raman peak in the Raman-silent region of cells, it is an interior label to visualize the nuclear location synchronously in real time when measuring the SERS spectra of a cell. Because no fluorescent-labeled dyes were used for locating cell nuclei, this method is simple, nondestructive, non- photobleaching, and valuable for the in situ exploration of vital physiological processes with DNA participation in cell organelles.

Rong Deng - One of the best experts on this subject based on the ideXlab platform.

  • in situ accurate surface enhanced raman scattering detection of cancer cell nucleus with synchronous location by an alkyne labeled Biomolecular Probe
    Analytical and Bioanalytical Chemistry, 2018
    Co-Authors: Jing Zhang, Lijia Liang, Xin Guan, Rong Deng, Dianshuai Huang, Chongyang Liang
    Abstract:

    A surface-enhanced Raman scattering (SERS) method for in situ detection and analysis of the intranuclear Biomolecular information of a cell has been developed based on a small, biocompatible, nuclear-targeting alkyne-tagged deoxyribonucleic acid (DNA) Probe (5-ethynyl-2'-deoxyuridine, EDU) that can specially accumulate in the cell nucleus during DNA replications to precisely locate the nuclear region without disturbance in cell biological activities and functions. Since the specific alkyne group shows a Raman peak in the Raman-silent region of cells, it is an interior label to visualize the nuclear location synchronously in real time when measuring the SERS spectra of a cell. Because no fluorescent-labeled dyes were used for locating cell nuclei, this method is simple, nondestructive, non- photobleaching, and valuable for the in situ exploration of vital physiological processes with DNA participation in cell organelles. Graphical abstract A universal strategy was developed to accurately locate the nuclear region and obtain precise molecular information of cell nuclei by SERS.

  • In situ, accurate, surface-enhanced Raman scattering detection of cancer cell nucleus with synchronous location by an alkyne-labeled Biomolecular Probe.
    Analytical and bioanalytical chemistry, 2017
    Co-Authors: Jing Zhang, Lijia Liang, Xin Guan, Rong Deng, Dianshuai Huang, Chongyang Liang
    Abstract:

    A surface-enhanced Raman scattering (SERS) method for in situ detection and analysis of the intranuclear Biomolecular information of a cell has been developed based on a small, biocompatible, nuclear-targeting alkyne-tagged deoxyribonucleic acid (DNA) Probe (5-ethynyl-2′-deoxyuridine, EDU) that can specially accumulate in the cell nucleus during DNA replications to precisely locate the nuclear region without disturbance in cell biological activities and functions. Since the specific alkyne group shows a Raman peak in the Raman-silent region of cells, it is an interior label to visualize the nuclear location synchronously in real time when measuring the SERS spectra of a cell. Because no fluorescent-labeled dyes were used for locating cell nuclei, this method is simple, nondestructive, non- photobleaching, and valuable for the in situ exploration of vital physiological processes with DNA participation in cell organelles.