The Experts below are selected from a list of 7569 Experts worldwide ranked by ideXlab platform
Mo Yang - One of the best experts on this subject based on the ideXlab platform.
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a fluorescence resonance energy transfer fret Biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca gene sequence of staphylococcus aureus
Biosensors and Bioelectronics, 2015Co-Authors: Jingyu Shi, Chunyu Chan, Yukting Pang, Feng Tian, Jing Lyu, Yu Zhang, Mo YangAbstract:In this work, a novel fluorescence resonance energy transfer (FRET) Biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET Biosensor Platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET Biosensor was around 1 nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET Biosensor.
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a fluorescence resonance energy transfer fret Biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca gene sequence of staphylococcus aureus
Biosensors and Bioelectronics, 2015Co-Authors: Chunyu Chan, Yukting Pang, Feng Tian, Yu Zhang, Weiwei Ye, Mo YangAbstract:Abstract In this work, a novel fluorescence resonance energy transfer (FRET) Biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET Biosensor Platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET Biosensor was around 1 nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET Biosensor.
Murugan Veerapandian - One of the best experts on this subject based on the ideXlab platform.
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graphene oxide functionalized with silver silica polyethylene glycol hybrid nanoparticles for direct electrochemical detection of quercetin
Biosensors and Bioelectronics, 2014Co-Authors: Murugan Veerapandian, Yeongtai Seo, Kyusik Yun, Minho LeeAbstract:Abstract A direct electrochemical detection of quercetin based on functionalized graphene oxide modified on gold-printed circuit board chip was demonstrated in this study. Functionalized graphene oxide materials are prepared by the covalent reaction of graphene oxide with silver@silica–polyethylene glycol nanoparticles (~12.35 nm). Functionalized graphene oxide electrode shows a well-defined voltammetric response in phosphate buffered saline and catalyzes the oxidation of quercetin to quinone without the need of an enzyme. Significantly, the functionalized graphene oxide modified electrode exhibited a higher sensitivity than pristine gold-printed circuit board and graphene oxide electrodes, a wide concentration range of 7.5 to 1040 nM and detection limit of 3.57 nM. Developed Biosensor Platform is selective toward quercetin in the presence of an interferent molecule.
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metalloid polymer nanoparticle functionalized graphene oxide working electrode for durable glucose sensing
Materials Research Bulletin, 2014Co-Authors: Murugan VeerapandianAbstract:Abstract A new class of functionalized graphene oxide (FGO) nanosheet based amperometric glucose Biosensor Platform has been fabricated. FGO nanosheet comprises of chemically bound metalloid polymer hybrid (MPH) nanoparticles (average size of 12.5 ±2 nm) on the surface of a graphene oxide (GO) nanosheet. Spectroscopic characterization indicated that MPHs are well distributed, with a strong binding affinity between the GO nanosheets. The synergistic features of the metalloid polymer and the GO resulted in a unique three-dimensional nano-architecture on a gold-printed circuit board electrode (Au-PCB). The electrocatalytic response against a glucose sample is predominant, with a characteristic response time of 7 s, correlation co-efficient of 0.9981 and a wide linear range of up to 55.5 mM. The stability of the nano-architecture modified on the electrode substrate is suitably durable for long-term application. The practical applicability of the fabricated electrode system was evaluated using a hyperglycemic clinical samples, and was compared with a commercial glucose Biosensor. The obtained amperometric results were in good agreement with those of the commercial Biosensor, and are promising for further clinical applications.
Chunyu Chan - One of the best experts on this subject based on the ideXlab platform.
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a fluorescence resonance energy transfer fret Biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca gene sequence of staphylococcus aureus
Biosensors and Bioelectronics, 2015Co-Authors: Jingyu Shi, Chunyu Chan, Yukting Pang, Feng Tian, Jing Lyu, Yu Zhang, Mo YangAbstract:In this work, a novel fluorescence resonance energy transfer (FRET) Biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET Biosensor Platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET Biosensor was around 1 nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET Biosensor.
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a fluorescence resonance energy transfer fret Biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca gene sequence of staphylococcus aureus
Biosensors and Bioelectronics, 2015Co-Authors: Chunyu Chan, Yukting Pang, Feng Tian, Yu Zhang, Weiwei Ye, Mo YangAbstract:Abstract In this work, a novel fluorescence resonance energy transfer (FRET) Biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET Biosensor Platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET Biosensor was around 1 nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET Biosensor.
Feng Tian - One of the best experts on this subject based on the ideXlab platform.
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a fluorescence resonance energy transfer fret Biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca gene sequence of staphylococcus aureus
Biosensors and Bioelectronics, 2015Co-Authors: Jingyu Shi, Chunyu Chan, Yukting Pang, Feng Tian, Jing Lyu, Yu Zhang, Mo YangAbstract:In this work, a novel fluorescence resonance energy transfer (FRET) Biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET Biosensor Platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET Biosensor was around 1 nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET Biosensor.
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a fluorescence resonance energy transfer fret Biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca gene sequence of staphylococcus aureus
Biosensors and Bioelectronics, 2015Co-Authors: Chunyu Chan, Yukting Pang, Feng Tian, Yu Zhang, Weiwei Ye, Mo YangAbstract:Abstract In this work, a novel fluorescence resonance energy transfer (FRET) Biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET Biosensor Platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET Biosensor was around 1 nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET Biosensor.
Yu Zhang - One of the best experts on this subject based on the ideXlab platform.
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a fluorescence resonance energy transfer fret Biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca gene sequence of staphylococcus aureus
Biosensors and Bioelectronics, 2015Co-Authors: Jingyu Shi, Chunyu Chan, Yukting Pang, Feng Tian, Jing Lyu, Yu Zhang, Mo YangAbstract:In this work, a novel fluorescence resonance energy transfer (FRET) Biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET Biosensor Platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET Biosensor was around 1 nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET Biosensor.
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a fluorescence resonance energy transfer fret Biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca gene sequence of staphylococcus aureus
Biosensors and Bioelectronics, 2015Co-Authors: Chunyu Chan, Yukting Pang, Feng Tian, Yu Zhang, Weiwei Ye, Mo YangAbstract:Abstract In this work, a novel fluorescence resonance energy transfer (FRET) Biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET Biosensor Platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET Biosensor was around 1 nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET Biosensor.