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Basil J. Nikolau - One of the best experts on this subject based on the ideXlab platform.

  • Differential Accumulation of Biotin Enzymes during Carrot Somatic Embryogenesis
    PLANT PHYSIOLOGY, 2008
    Co-Authors: Eve Syrkin Wurtele, Basil J. Nikolau
    Abstract:

    The activities of four Biotin Enzymes, acetyl-coenzyme A (CoA) carboxylase, 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase, and the accumulation of six Biotin-containing polypeptides were determined during development of somatic embryos of carrot (Daucus carota). Acetyl-CoA carboxylase activity increased more than sevenfold, whereas the activities of 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase were relatively unaltered. An increase also occurred in the accumulation of three of the Biotin-containing polypeptides (molecular masses of 220, 62, and 34 kilodaltons). Of these, the most dramatic change was in the accumulation of the 62-kilodalton Biotin-containing polypeptide, which increased by at least 50-fold as embryogenic cell clusters developed into torpedo embryos.

Alberto M. Vargas - One of the best experts on this subject based on the ideXlab platform.

  • Evolution of pyruvate carboxylase and other Biotin containing Enzymes in developing rat liver and kidney
    Molecular and Cellular Biochemistry, 1999
    Co-Authors: Rafael Salto, María Del Mar Sola, María D. Girón, Alberto M. Vargas
    Abstract:

    The evolution of pyruvate carboxylase has been studied in rat liver and kidney during perinatal development. The pyruvate carboxylase activity, amount of enzyme and mRNA levels have been assayed from 2 days before delivery to weaning. In liver, there is a peak of activity and amount of enzyme 24 h before delivery and 2 peaks, at 12 h and 6 days, after parturition. The transcription of the enzyme gene followed a similar pattern, with mRNA peaks preceding those of activity and amount of enzyme. However, in kidney, pyruvate carboxylase activity, amount and mRNA remain low until weaning. These results confirm the limited role of renal gluconeogenesis during the perinatal development. Since all carboxylases contain Biotin as prosthetic group, the Biotinylation of pyruvate carboxylase during the perinatal period was investigated by western-blot using streptavidin-Biotin peroxidase. In the mitochondrial samples from liver and kidney, all the pyruvate carboxylase detected was fully Biotinylated, indicating an early development of the holocarboxylase synthetase activity in the perinatal period. This Western-blot technique also allowed us the detection of other Biotin-Enzymes based on their molecular weight. In liver, during the perinatal development propionyl-coA and 3-methyl-crotonyl-coA carboxylases followed a pattern of induction similar to pyruvate carboxylase. In kidney, the expression of mitochondrial carboxylases was lower compared to liver and propionyl-coA carboxylase was not detected during the studied period

Eve Syrkin Wurtele - One of the best experts on this subject based on the ideXlab platform.

  • Differential Accumulation of Biotin Enzymes during Carrot Somatic Embryogenesis
    PLANT PHYSIOLOGY, 2008
    Co-Authors: Eve Syrkin Wurtele, Basil J. Nikolau
    Abstract:

    The activities of four Biotin Enzymes, acetyl-coenzyme A (CoA) carboxylase, 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase, and the accumulation of six Biotin-containing polypeptides were determined during development of somatic embryos of carrot (Daucus carota). Acetyl-CoA carboxylase activity increased more than sevenfold, whereas the activities of 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase were relatively unaltered. An increase also occurred in the accumulation of three of the Biotin-containing polypeptides (molecular masses of 220, 62, and 34 kilodaltons). Of these, the most dramatic change was in the accumulation of the 62-kilodalton Biotin-containing polypeptide, which increased by at least 50-fold as embryogenic cell clusters developed into torpedo embryos.

Rafael Salto - One of the best experts on this subject based on the ideXlab platform.

  • Evolution of pyruvate carboxylase and other Biotin containing Enzymes in developing rat liver and kidney
    Molecular and Cellular Biochemistry, 1999
    Co-Authors: Rafael Salto, María Del Mar Sola, María D. Girón, Alberto M. Vargas
    Abstract:

    The evolution of pyruvate carboxylase has been studied in rat liver and kidney during perinatal development. The pyruvate carboxylase activity, amount of enzyme and mRNA levels have been assayed from 2 days before delivery to weaning. In liver, there is a peak of activity and amount of enzyme 24 h before delivery and 2 peaks, at 12 h and 6 days, after parturition. The transcription of the enzyme gene followed a similar pattern, with mRNA peaks preceding those of activity and amount of enzyme. However, in kidney, pyruvate carboxylase activity, amount and mRNA remain low until weaning. These results confirm the limited role of renal gluconeogenesis during the perinatal development. Since all carboxylases contain Biotin as prosthetic group, the Biotinylation of pyruvate carboxylase during the perinatal period was investigated by western-blot using streptavidin-Biotin peroxidase. In the mitochondrial samples from liver and kidney, all the pyruvate carboxylase detected was fully Biotinylated, indicating an early development of the holocarboxylase synthetase activity in the perinatal period. This Western-blot technique also allowed us the detection of other Biotin-Enzymes based on their molecular weight. In liver, during the perinatal development propionyl-coA and 3-methyl-crotonyl-coA carboxylases followed a pattern of induction similar to pyruvate carboxylase. In kidney, the expression of mitochondrial carboxylases was lower compared to liver and propionyl-coA carboxylase was not detected during the studied period

María Del Mar Sola - One of the best experts on this subject based on the ideXlab platform.

  • Evolution of pyruvate carboxylase and other Biotin containing Enzymes in developing rat liver and kidney
    Molecular and Cellular Biochemistry, 1999
    Co-Authors: Rafael Salto, María Del Mar Sola, María D. Girón, Alberto M. Vargas
    Abstract:

    The evolution of pyruvate carboxylase has been studied in rat liver and kidney during perinatal development. The pyruvate carboxylase activity, amount of enzyme and mRNA levels have been assayed from 2 days before delivery to weaning. In liver, there is a peak of activity and amount of enzyme 24 h before delivery and 2 peaks, at 12 h and 6 days, after parturition. The transcription of the enzyme gene followed a similar pattern, with mRNA peaks preceding those of activity and amount of enzyme. However, in kidney, pyruvate carboxylase activity, amount and mRNA remain low until weaning. These results confirm the limited role of renal gluconeogenesis during the perinatal development. Since all carboxylases contain Biotin as prosthetic group, the Biotinylation of pyruvate carboxylase during the perinatal period was investigated by western-blot using streptavidin-Biotin peroxidase. In the mitochondrial samples from liver and kidney, all the pyruvate carboxylase detected was fully Biotinylated, indicating an early development of the holocarboxylase synthetase activity in the perinatal period. This Western-blot technique also allowed us the detection of other Biotin-Enzymes based on their molecular weight. In liver, during the perinatal development propionyl-coA and 3-methyl-crotonyl-coA carboxylases followed a pattern of induction similar to pyruvate carboxylase. In kidney, the expression of mitochondrial carboxylases was lower compared to liver and propionyl-coA carboxylase was not detected during the studied period