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M C Navarromoll - One of the best experts on this subject based on the ideXlab platform.
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activity of matricaria chamomilla essential oil against anisakiasis
Phytomedicine, 2012Co-Authors: Maria Del Carmen Molina Romero, A Valero, Joaquina Martinsanchez, M C NavarromollAbstract:Abstract The increase in diagnosed cases of anisakiasis and the virtual absence of effective treatments have prompted the search for new active compounds against Anisakis L3 larvae. The biocidal efficacy against different pathogens shown by various essential oils (EO) led us to study the Matricaria chamomilla EO and two of its main components (chamazulene and α-Bisabolol) against the L3 larvae of Anisakis type I. The activity of M. chamomilla EO, chamazulene and α-Bisabolol was established by in vitro and in vivo experiments. The EO (125 μg/ml) caused the death of all nematodes, which showed cuticle changes and intestinal wall rupture. In the in vivo assays, only 2.2% ± 1.8 of infected rats treated with M. chamomilla EO showed gastric wall lesions in comparison to 93.3% ± 3.9 of control. Chamazulene was ineffective, while α-Bisabolol showed a high activity to that of the EO in vitro tests but proved less active in vivo. These findings suggest that the larvicidal activity may result from the synergistic action of different compounds of M. chamomilla EO. Neither of the tested products induces irritative damage in the intestinal tissues. In conclusion, M. chamomilla EO is a good candidate for further investigation as a biocidal agent against Anisakis type I.
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activity of α Bisabolol against leishmania infantum promastigotes
Phytomedicine, 2010Co-Authors: Manuel Moralesyuste, Joaquina Martinsanchez, F Morillasmarquez, A Valerolopez, M C NavarromollAbstract:Many of the drugs used to treat leishmaniasis are associated with numerous adverse effects. Agents of natural origin have shown activity against different parasites. With this background, an in vitro study was conducted on the activity of (-)α-Bisabolol, the principal component of Chamomilla recutita essential oil, against Leishmania infantum promastigotes, the main species responsible for human leishmaniasis in Spain. At the two highest concentrations tested (1000 and 500 μg/ml), (-)α-Bisabolol and pentamidine (control agent) achieved 100% inhibition of L. infantum promastigote. These in vitro data can be considered promising in support of the therapeutic use of (-)α-Bisabolol preparations to treat leishmaniasis caused by L. infantum species.
Edson Antonio Da Silva - One of the best experts on this subject based on the ideXlab platform.
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candeia eremanthus erythroppapus oil extraction using supercritical co2 with ethanol and ethyl acetate cosolvents
Journal of Supercritical Fluids, 2017Co-Authors: Katia Andressa Santos, Paula Cassiana Frohlich, Jaqueline Hoscheid, Tatiana Shioji Tiuman, Jose Eduardo Goncalves, Lucio Cardozofilho, Edson Antonio Da SilvaAbstract:Abstract This study evaluated the candeia wood (Eremanthus erythropappus) oil extraction using supercritical extraction with CO2 and the addition of ethanol and ethyl acetate as cosolvents (1, 3 and 5% v/v). The experiments were performed at 70 °C and 24 MPa, with a mixture volumetric flow of 2 mL min−1. The cosolvents increased the yield of extraction, obtaining up to 2.35 wt% with the addition of 5% ethanol, approximately 52% larger than the extraction with CO2. The extraction kinetic curves were satisfactorily represented by the Sovova mathematical model. In the extraction with 5% ethanol, it was possible to obtain the highest yield of α-Bisabolol, 16.53 g kg−1, an amount 41% higher than the extraction without cosolvent. The addition of ethanol and ethyl acetate to CO2 increased the amount of total phenolic content in the oil, and consequently, its antioxidant capacity. The oil fractionation by column chromatography was efficient for α-Bisabolol isolation.
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wood and industrial residue of candeia eremanthus erythropappus supercritical co2 oil extraction composition antioxidant activity and mathematical modeling
Journal of Supercritical Fluids, 2016Co-Authors: Katia Andressa Santos, Jose Eduardo Goncalves, Lucio Cardozofilho, Elissandro Jair Klein, Zilda Cristiani Gazim, Marcos L Corazza, Edson Antonio Da SilvaAbstract:Abstract This study evaluated the extraction of oil from candeia wood (Eremanthus erythropappus) and industrial residue using supercritical CO2. Extractions were carried out at 40, 55 and 70 °C and 160, 200 and 240 bar, with a mass flow rate of 1.96 × 10−3 kg min−1. The oils were characterized for α-Bisabolol content, chemical composition and antioxidant activity. The highest extraction yield obtained was 1.42 wt% for the candeia wood and 0.41 wt% for the residue, both at 70 °C and 240 bar. The Sovova model was used to represent the experimental kinetics of extraction. The major compounds identified in the candeia oil were the sesquiterpenes α-Bisabolol, eremanthin and costunolide, and the highest α-Bisabolol content (74.45 g from 100 g of oil) was obtained by the lowest density of the solvent. The highest values for antioxidant activity, determined by the DPPH, FRAP and ABTS methods, were found for oil extracted at 70 °C and 240 bar.
Katia Andressa Santos - One of the best experts on this subject based on the ideXlab platform.
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candeia eremanthus erythroppapus oil extraction using supercritical co2 with ethanol and ethyl acetate cosolvents
Journal of Supercritical Fluids, 2017Co-Authors: Katia Andressa Santos, Paula Cassiana Frohlich, Jaqueline Hoscheid, Tatiana Shioji Tiuman, Jose Eduardo Goncalves, Lucio Cardozofilho, Edson Antonio Da SilvaAbstract:Abstract This study evaluated the candeia wood (Eremanthus erythropappus) oil extraction using supercritical extraction with CO2 and the addition of ethanol and ethyl acetate as cosolvents (1, 3 and 5% v/v). The experiments were performed at 70 °C and 24 MPa, with a mixture volumetric flow of 2 mL min−1. The cosolvents increased the yield of extraction, obtaining up to 2.35 wt% with the addition of 5% ethanol, approximately 52% larger than the extraction with CO2. The extraction kinetic curves were satisfactorily represented by the Sovova mathematical model. In the extraction with 5% ethanol, it was possible to obtain the highest yield of α-Bisabolol, 16.53 g kg−1, an amount 41% higher than the extraction without cosolvent. The addition of ethanol and ethyl acetate to CO2 increased the amount of total phenolic content in the oil, and consequently, its antioxidant capacity. The oil fractionation by column chromatography was efficient for α-Bisabolol isolation.
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wood and industrial residue of candeia eremanthus erythropappus supercritical co2 oil extraction composition antioxidant activity and mathematical modeling
Journal of Supercritical Fluids, 2016Co-Authors: Katia Andressa Santos, Jose Eduardo Goncalves, Lucio Cardozofilho, Elissandro Jair Klein, Zilda Cristiani Gazim, Marcos L Corazza, Edson Antonio Da SilvaAbstract:Abstract This study evaluated the extraction of oil from candeia wood (Eremanthus erythropappus) and industrial residue using supercritical CO2. Extractions were carried out at 40, 55 and 70 °C and 160, 200 and 240 bar, with a mass flow rate of 1.96 × 10−3 kg min−1. The oils were characterized for α-Bisabolol content, chemical composition and antioxidant activity. The highest extraction yield obtained was 1.42 wt% for the candeia wood and 0.41 wt% for the residue, both at 70 °C and 240 bar. The Sovova model was used to represent the experimental kinetics of extraction. The major compounds identified in the candeia oil were the sesquiterpenes α-Bisabolol, eremanthin and costunolide, and the highest α-Bisabolol content (74.45 g from 100 g of oil) was obtained by the lowest density of the solvent. The highest values for antioxidant activity, determined by the DPPH, FRAP and ABTS methods, were found for oil extracted at 70 °C and 240 bar.
Angela M A Meireles - One of the best experts on this subject based on the ideXlab platform.
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supercritical co2 extraction of essential oil and oleoresin from chamomile chamomilla recutita l rauschert
Journal of Supercritical Fluids, 2001Co-Authors: Nanci Pinheiro Povh, Marcia Ortiz Mayo Marques, Angela M A MeirelesAbstract:Abstract Flowers of chamomile ( Chamomilla recutita [L.] Rauschert) were extracted with supercritical carbon dioxide using a fixed bed extractor. The experimental work was conducted to identify the best process conditions to maximize the yield of extract and its content of α-Bisabolol and chamazulene. The experimental setup used a fixed bed extractor (diameter of 3.96×10 −2 m and length 16.55×10 −2 m). The fixed bed was formed with triturated chamomile (−28 to +200 mesh) with an apparent density of 370 kg/m 3 . Assays were conducted at temperatures of 30 and 40 °C, pressures of 100, 120, 160 and 200 bar. The solvent flow rates were 1.67×10 −5 , 3.33×10 −5 and 6.67×10 −5 kg/s. A typical run took 10 h. The chemical composition of the extracts was determined using GC/MS and GC. The pressure and solvent flow rate significantly affected the mass transfer rate and the yield while the temperature did not. The maximum yield (mass of extract/mass of dried feed) was 4.33% (40 °C, 200 bar, 6.67×10 −5 kg/s). The overall extraction curves were well described by both a spline fitting and the pseudo steady state model of Sovova. The major compounds of the essential oil and of the oleoresin were β-farnesene, α-farnesene, γ-cadinene, α-Bisabolol oxide B, α-Bisabolol, chamazulene, α-Bisabolol oxide A, cis and trans -dicycloether (MW 200).
Dae-kyun Ro - One of the best experts on this subject based on the ideXlab platform.
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enantioselective microbial synthesis of the indigenous natural product α Bisabolol by a sesquiterpene synthase from chamomile matricaria recutita
Biochemical Journal, 2014Co-Authors: Moonhyuk Kwon, Dae-kyun RoAbstract:(−)-α-Bisabolol, a sesquiterpene alcohol, is a major ingredient in the essential oil of chamomile ( Matricaria recutita ) and is used in many health products. The current supply of (−)-α-Bisabolol is mainly dependent on the Brazilian candeia tree ( Eremanthus erythropappus ) by distillation or by chemical synthesis. However, the distillation method using the candeia tree is not sustainable, and chemical synthesis suffers from impurities arising from undesirable α-Bisabolol isomers. Therefore enzymatic synthesis of (−)-α-Bisabolol is a viable alternative. In the present study, a cDNA encoding (−)-α-Bisabolol synthase ( MrBBS ) was identified from chamomile and used for enantioselective (−)-α-Bisabolol synthesis in yeast. Chamomile MrBBS was identified by Illumina and 454 sequencing, followed by activity screening in yeast. When MrBBS was expressed in yeast, 8 mg of α-Bisabolol was synthesized de novo per litre of culture. The structure of purified α-Bisabolol was elucidated as ( S , S )-α-Bisabolol [or (−)-α-Bisabolol]. Although MrBBS possesses a putative chloroplast-targeting peptide, it was localized in the cytosol, and a deletion of its N-terminal 23 amino acids significantly reduced its stability and activity. Recombinant MrBBS showed kinetic properties comparable with those of other sesquiterpene synthases. These data provide compelling evidence that chamomile MrBBS synthesizes enantiopure (−)-α-Bisabolol as a single sesquiterpene product, opening a biotechnological opportunity to produce (−)-α-Bisabolol. Abbreviations: DMAPP, dimethyl allyl diphosphate; EI, electron impact; FPP, farnesyl diphosphate; FPPS, FPP synthase; IPP, isopentenyl diphosphate; LdBBS, (+)-epi-α-Bisabolol synthase from Lippia dulcis; MBP, maltose-binding protein; mono-TPS, monoterpene synthase; MrBBS, chamomile (Matricaria recutita) α-Bisabolol synthase; MrTPS, chamomile (Matricaria recutita) TPS; MVA, mevalonate; NPP, nerolidyl diphosphate; qRT-PCR, quantitative reverse transcription–PCR; SC, synthetic complete; sesqui-TPS, sesquiterpene synthase; TPS, terpene synthase; TRX, thioredoxin