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Bidyut Kumar Sarmah – 1st expert on this subject based on the ideXlab platform
An efficient in vitro regeneration protocol to generate stable transgenic lines of Black Gram (Vigna mungo)Indian Journal of Genetics and Plant Breeding, 2018Co-Authors: Bidyut Kumar Sarmah, Trishna Konwar, Borsha Borah, Arun Kumar Handique, Sumita AcharjeeAbstract:
An efficient and quick in vitro regeneration protocol was developed for Black Gram (Vigna mungo) using wounded embryonic axis with cotyledon as explant. Murashige and Skoog (MS) medium supplemented with 4.44 μM BAP and 2.32 μM Kinetin was found to be effective in producing maximum number (mean 7.80) of multiple shoots. The individual shoots elongated to 4.5 cm when MS medium was supplemented with 2.89 μM GA3 along with 0.44 μM BAP and 0.46 μM KIN. A novel in vitro rooting technique was also optimized for Black Gram using half-strength liquid MS medium supplemented with 1.34 μM NAA. The shoots in this medium produced the highest number (mean 7.50) of roots with root length of 6.02 cm. The plantlets were transferred to soil mixture and placed in greenhouse where more than 80% successfully grew to maturity. The same protocol was successfully used to generate transgenic Black Gram lines carrying Bt-Cry2Aa gene through Agrobacteriummediated transformation with a transformation efficiency of 0.42%. The rooted T0 plants grew to maturity and produced T1 seeds with the presence and expression of transgene in T1 plants. Thus, we have standardized an in vitro regeneration protocol suitable for generation of stable transgenic Black Gram plants.
bruchid egg induced transcript dynamics in developing seeds of Black Gram vigna mungoPLOS ONE, 2017Co-Authors: Indrani K Baruah, Debashis Panda, Jagadale M, Sumita Acharjee, Bidyut Kumar SarmahAbstract:
: Black Gram (Vigna mungo) seeds are a rich source of digestible proteins, however, during storage these seeds are severely damaged by bruchids (Callosobruchus spp.), reducing seed quality and yield losses. Most of the cultivated genotypes of Black Gram are susceptible to bruchids, however, few tolerant genotypes have also been identified but the mechanism of tolerance is poorly understood. We employed Suppression Subtractive Hybridization (SSH) to identify specifically, but rarely expressed bruchid egg induced genes in Black Gram. In this study, Suppression Subtractive Hybridization (SSH) library was constructed to study the genes involved in defense response in Black Gram against bruchid infestation. An EST library of 277 clones was obtained for further analyses. Based on CAP3 assembly, 134 unigenes were computationally annotated using Blast2GOPRO software. In all, 20 defense related genes were subject to quantitative PCR analysis (qPCR) out of which 12 genes showed up-regulation in developing seeds of the pods oviposited by bruchids. Few major defense genes like defensin, pathogenesis related protein (PR), lipoxygenase (LOX) showed high expression levels in the oviposited population when compared with the non-oviposited plants. This is the first report on defense related gene transcript dynamics during the bruchid-Black Gram interaction using SSH library. This library would be useful to clone defense related gene(s) such as defensin as represented in our library for crop improvement.
S A Haider – 2nd expert on this subject based on the ideXlab platform
effect of different rhizobium strains on the growth attributes of Black GramJournal of Life and Earth Science, 2011Co-Authors: Sk Nitu, M M Uddeen, S A HaiderAbstract:
Effects of three different Rhizobium strains on the growth attributes of two Black Gram varieties ( Vigna mungo L. Hepper) revealed differential varietal response due to inoculation with two types of Rhizobium strains. Treatment T 5 showed higher values compared to the rest of the treatments. Different growth parameters such as RGR, LAR, RLGR, LAD, LAI, SLA and LWR indicated better results with the inoculation of nodule organism. Key words : Growth attributes; Black Gram ; Vigna mungo; Rhizobium strains DOI: 10.3329/jles.v3i0.7438 J. Life Earth Sci., Vols. 3-4: 7-11, 2009
Peerasak Srinives – 3rd expert on this subject based on the ideXlab platform
genetic diversity of the Black Gram vigna mungo l hepper gene pool as revealed by ssr markersBreeding Science, 2015Co-Authors: Anochar Kaewwongwal, Alisa Kongjaimun, Prakit Somta, Sompong Chankaew, Tarikar Yimram, Peerasak SrinivesAbstract:
In this study, 520 cultivated and 14 wild accessions of Black Gram (Vigna mungo (L.) Hepper) were assessed for diversity using 22 SSR markers. Totally, 199 alleles were detected with a mean of 9.05 alleles per locus. Wild Black Gram showed higher gene diversity than cultivated Black Gram. Gene diversity of cultivated accessions among regions was comparable, while allelic richness of South Asia was higher than that of other regions. 78.67% of the wild gene diversity presented in cultivated accessions, indicating that the domestication bottleneck effect in Black Gram is relatively low. Genetic distance analysis revealed that cultivated Black Gram was more closely related to wild Black Gram from South Asia than that from Southeast Asia. STRUCTURE, principal coordinate and neighbor-joining analyses consistently revealed that 534 Black Gram accessions were grouped into three major subpopulations. The analyses also revealed that cultivated Black Gram from South Asia was genetically distinct from that from West Asia. Comparison by SSR analysis with other closely related Vigna species, including mungbean, azuki bean, and rice bean, revealed that level of gene diversity of Black Gram is comparable to that of mungbean and rice bean but lower than that of azuki bean.