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Limin Xiang - One of the best experts on this subject based on the ideXlab platform.

  • potential anti inflammatory steroidal saponins from the berries of solanum nigrum l european Black Nightshade
    Journal of Agricultural and Food Chemistry, 2017
    Co-Authors: Yihai Wang, Limin Xiang
    Abstract:

    Solanum nigrum L. or European Black Nightshade (Solanum genus) is a common weed of crops and gardens. The berries and leaves of S. nigrum L. are consumed as food or vegetable in some regions and reported to possess a range of biological activities. In this study, nine new steroidal saponins, solanigrosides Y1–Y9 (1–6, 10–12), together with seven known congeners, were isolated from the berries of S. nigrum. Their potential inhibitory effects on nitric oxide (NO) and IL-6 and IL-1β production induced by lipopolysaccharide (LPS) in macrophages cell line RAW 264.7 were evaluated. Compound 1 exhibited significant inhibition on NO production with an IC50 value of 9.7 μM, and some compounds exhibited significant inhibition effects on the LPS-induced IL-6 and IL-1β production. These results suggest that the steroidal saponins from berries of S. nigrum demonstrated pronounced anti-inflammatory activity and might be explored as a healthy benefit agent.

  • Potential Anti-inflammatory Steroidal Saponins from the Berries of Solanum nigrum L. (European Black Nightshade)
    2017
    Co-Authors: Yihai Wang, Limin Xiang
    Abstract:

    Solanum nigrum L. or European Black Nightshade (Solanum genus) is a common weed of crops and gardens. The berries and leaves of S. nigrum L. are consumed as food or vegetable in some regions and reported to possess a range of biological activities. In this study, nine new steroidal saponins, solanigrosides Y1–Y9 (1–6, 10–12), together with seven known congeners, were isolated from the berries of S. nigrum. Their potential inhibitory effects on nitric oxide (NO) and IL-6 and IL-1β production induced by lipopolysaccharide (LPS) in macrophages cell line RAW 264.7 were evaluated. Compound 1 exhibited significant inhibition on NO production with an IC50 value of 9.7 μM, and some compounds exhibited significant inhibition effects on the LPS-induced IL-6 and IL-1β production. These results suggest that the steroidal saponins from berries of S. nigrum demonstrated pronounced anti-inflammatory activity and might be explored as a healthy benefit agent

Yihai Wang - One of the best experts on this subject based on the ideXlab platform.

  • potential anti inflammatory steroidal saponins from the berries of solanum nigrum l european Black Nightshade
    Journal of Agricultural and Food Chemistry, 2017
    Co-Authors: Yihai Wang, Limin Xiang
    Abstract:

    Solanum nigrum L. or European Black Nightshade (Solanum genus) is a common weed of crops and gardens. The berries and leaves of S. nigrum L. are consumed as food or vegetable in some regions and reported to possess a range of biological activities. In this study, nine new steroidal saponins, solanigrosides Y1–Y9 (1–6, 10–12), together with seven known congeners, were isolated from the berries of S. nigrum. Their potential inhibitory effects on nitric oxide (NO) and IL-6 and IL-1β production induced by lipopolysaccharide (LPS) in macrophages cell line RAW 264.7 were evaluated. Compound 1 exhibited significant inhibition on NO production with an IC50 value of 9.7 μM, and some compounds exhibited significant inhibition effects on the LPS-induced IL-6 and IL-1β production. These results suggest that the steroidal saponins from berries of S. nigrum demonstrated pronounced anti-inflammatory activity and might be explored as a healthy benefit agent.

  • Potential Anti-inflammatory Steroidal Saponins from the Berries of Solanum nigrum L. (European Black Nightshade)
    2017
    Co-Authors: Yihai Wang, Limin Xiang
    Abstract:

    Solanum nigrum L. or European Black Nightshade (Solanum genus) is a common weed of crops and gardens. The berries and leaves of S. nigrum L. are consumed as food or vegetable in some regions and reported to possess a range of biological activities. In this study, nine new steroidal saponins, solanigrosides Y1–Y9 (1–6, 10–12), together with seven known congeners, were isolated from the berries of S. nigrum. Their potential inhibitory effects on nitric oxide (NO) and IL-6 and IL-1β production induced by lipopolysaccharide (LPS) in macrophages cell line RAW 264.7 were evaluated. Compound 1 exhibited significant inhibition on NO production with an IC50 value of 9.7 μM, and some compounds exhibited significant inhibition effects on the LPS-induced IL-6 and IL-1β production. These results suggest that the steroidal saponins from berries of S. nigrum demonstrated pronounced anti-inflammatory activity and might be explored as a healthy benefit agent

Jan Rezek - One of the best experts on this subject based on the ideXlab platform.

  • metabolites of 2 2 dichlorobiphenyl and 2 6 dichlorobiphenyl in hairy root culture of Black Nightshade solanum nigrum snc 9o
    Chemosphere, 2012
    Co-Authors: Jan Rezek, Tomas Macek, Jan Doubsky, Martina Mackova
    Abstract:

    Abstract The hairy root culture of Black Nightshade ( Solanum nigrum ) SNC-9O was exposed to 2,2′-dichlorobiphenyl (PCB 4) and 2,6-dichlorobiphenyl (PCB 10) to follow the metabolites produced. The analytical standards of 4-hydroxy-2,2′-dichlorobiphenyl, 5′-hydroxy-2,2′-dichlorobiphenyl, 4-hydroxy-2,6-dichlorobiphenyl, 2-hydroxy-2′,6′-dichlorobiphenyl, 3-hydroxy-2′,6′-dichlorobiphenyl and 4-hydroxy-2′,6′-dichlorobiphenyl have been synthesized. Hydroxy-metabolites of both PCB 4 and PCB 10 were present in the biomass. These appeared mainly as conjugates rather than as free hydroxy-PCBs, both maintained in plant cells. The concentrations of non-conjugated hydroxy-PCBs ranged between 0.9 and 35.2 μg kg −1 of biomass fresh weight and the concentration of the conjugated ones ranged between 2.0 and 113.0 μg kg −1 depending on the position of hydroxyl. The para- position of biphenyl (4 or 4′) seems to be preferred for hydroxylation. Methoxy-PCBs and hydroxy–methoxy-PCBs have also been identified in plant cells. Hydroxyl in the meta- position (3, 3′, 5 or 5′) appears to be preferred for methylation in hydroxy-PCBs. Hydroxy–methoxy-PCBs have occurred in the conjugated form as well.

  • plant metabolites of polychlorinated biphenyls in hairy root culture of Black Nightshade solanum nigrum snc 9o
    Chemosphere, 2007
    Co-Authors: Jan Rezek, Martina Mackova, Tomas Macek, Jan Triska
    Abstract:

    Abstract The present study is intended to determine metabolites of 12 dichlorinated, seven trichlorinated, five tetrachlorinated and one pentachlorinated PCB congener transformed by Black Nightshade ( Solanum nigrum ) hairy root culture SNC-9O. Free hydroxylated PCB metabolites were identified based on the mass spectra characteristics after gas chromatography separation. The number of metabolites decreases with an increasing number of chlorine atoms per molecule of PCB. Dichlorinated PCBs lead always to at least two metabolites. In the case of PCB 9 some metabolites could be identified by comparing their RF values due to available standards. The 2′,5′-dichloro-2-biphenylol, 2′,5′-dichloro-3-biphenylol and 2′,5′-dichloro-4-biphenylol, present as the main metabolite, were found in biomass of SNC-9O hairy root culture. Two monochlorinated biphenylols were found in biomass of SNC-9O degrading PCB 9 congener. It was the only case when metabolites with decreased number of chlorine atoms compared to parent PCB were found. Trichlorinated PCBs mostly lead to a lower number of metabolites but tetrachlorinated and pentachlorinated PCBs mostly did not give any metabolites. In the media, only traces of metabolites were found in sporadic cases, so exudation of unbound biphenylols from the cells is not expected.

Martina Mackova - One of the best experts on this subject based on the ideXlab platform.

  • metabolites of 2 2 dichlorobiphenyl and 2 6 dichlorobiphenyl in hairy root culture of Black Nightshade solanum nigrum snc 9o
    Chemosphere, 2012
    Co-Authors: Jan Rezek, Tomas Macek, Jan Doubsky, Martina Mackova
    Abstract:

    Abstract The hairy root culture of Black Nightshade ( Solanum nigrum ) SNC-9O was exposed to 2,2′-dichlorobiphenyl (PCB 4) and 2,6-dichlorobiphenyl (PCB 10) to follow the metabolites produced. The analytical standards of 4-hydroxy-2,2′-dichlorobiphenyl, 5′-hydroxy-2,2′-dichlorobiphenyl, 4-hydroxy-2,6-dichlorobiphenyl, 2-hydroxy-2′,6′-dichlorobiphenyl, 3-hydroxy-2′,6′-dichlorobiphenyl and 4-hydroxy-2′,6′-dichlorobiphenyl have been synthesized. Hydroxy-metabolites of both PCB 4 and PCB 10 were present in the biomass. These appeared mainly as conjugates rather than as free hydroxy-PCBs, both maintained in plant cells. The concentrations of non-conjugated hydroxy-PCBs ranged between 0.9 and 35.2 μg kg −1 of biomass fresh weight and the concentration of the conjugated ones ranged between 2.0 and 113.0 μg kg −1 depending on the position of hydroxyl. The para- position of biphenyl (4 or 4′) seems to be preferred for hydroxylation. Methoxy-PCBs and hydroxy–methoxy-PCBs have also been identified in plant cells. Hydroxyl in the meta- position (3, 3′, 5 or 5′) appears to be preferred for methylation in hydroxy-PCBs. Hydroxy–methoxy-PCBs have occurred in the conjugated form as well.

  • plant metabolites of polychlorinated biphenyls in hairy root culture of Black Nightshade solanum nigrum snc 9o
    Chemosphere, 2007
    Co-Authors: Jan Rezek, Martina Mackova, Tomas Macek, Jan Triska
    Abstract:

    Abstract The present study is intended to determine metabolites of 12 dichlorinated, seven trichlorinated, five tetrachlorinated and one pentachlorinated PCB congener transformed by Black Nightshade ( Solanum nigrum ) hairy root culture SNC-9O. Free hydroxylated PCB metabolites were identified based on the mass spectra characteristics after gas chromatography separation. The number of metabolites decreases with an increasing number of chlorine atoms per molecule of PCB. Dichlorinated PCBs lead always to at least two metabolites. In the case of PCB 9 some metabolites could be identified by comparing their RF values due to available standards. The 2′,5′-dichloro-2-biphenylol, 2′,5′-dichloro-3-biphenylol and 2′,5′-dichloro-4-biphenylol, present as the main metabolite, were found in biomass of SNC-9O hairy root culture. Two monochlorinated biphenylols were found in biomass of SNC-9O degrading PCB 9 congener. It was the only case when metabolites with decreased number of chlorine atoms compared to parent PCB were found. Trichlorinated PCBs mostly lead to a lower number of metabolites but tetrachlorinated and pentachlorinated PCBs mostly did not give any metabolites. In the media, only traces of metabolites were found in sporadic cases, so exudation of unbound biphenylols from the cells is not expected.

Jan Triska - One of the best experts on this subject based on the ideXlab platform.

  • plant metabolites of polychlorinated biphenyls in hairy root culture of Black Nightshade solanum nigrum snc 9o
    Chemosphere, 2007
    Co-Authors: Jan Rezek, Martina Mackova, Tomas Macek, Jan Triska
    Abstract:

    Abstract The present study is intended to determine metabolites of 12 dichlorinated, seven trichlorinated, five tetrachlorinated and one pentachlorinated PCB congener transformed by Black Nightshade ( Solanum nigrum ) hairy root culture SNC-9O. Free hydroxylated PCB metabolites were identified based on the mass spectra characteristics after gas chromatography separation. The number of metabolites decreases with an increasing number of chlorine atoms per molecule of PCB. Dichlorinated PCBs lead always to at least two metabolites. In the case of PCB 9 some metabolites could be identified by comparing their RF values due to available standards. The 2′,5′-dichloro-2-biphenylol, 2′,5′-dichloro-3-biphenylol and 2′,5′-dichloro-4-biphenylol, present as the main metabolite, were found in biomass of SNC-9O hairy root culture. Two monochlorinated biphenylols were found in biomass of SNC-9O degrading PCB 9 congener. It was the only case when metabolites with decreased number of chlorine atoms compared to parent PCB were found. Trichlorinated PCBs mostly lead to a lower number of metabolites but tetrachlorinated and pentachlorinated PCBs mostly did not give any metabolites. In the media, only traces of metabolites were found in sporadic cases, so exudation of unbound biphenylols from the cells is not expected.