The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform
Elizabeth Burcher - One of the best experts on this subject based on the ideXlab platform.
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Acid and stretch, but not capsaicin, are effective stimuli for ATP release in the porcine Bladder Mucosa: Are ASIC and TRPV1 receptors involved?
European journal of pharmacology, 2012Co-Authors: Prajni Sadananda, Kylie J Mansfield, Felicity C L Kao, Lu Liu, Elizabeth BurcherAbstract:Stretch-evoked ATP release from the Bladder Mucosa is a key event in signaling Bladder fullness. Our aim was to examine whether acid and capsaicin can also release ATP and to determine the receptors involved, using agonists and antagonists at TRPV1 and acid-sensing ion channels (ASICs). Strips of porcine Bladder Mucosa were exposed to acid, capsaicin or stretch. Strip tension was monitored. Bath fluid was collected for ATP measurement. Gene expression of ASICs and TRPV1 in porcine Bladders was quantified using quantitative real-time PCR (qRT-PCR). Stretch stimulus (150% of original length) repeatedly and significantly increased ATP release to approximately 45 times basal release. Acid (pH 6.5, 6.0, 5.6) contracted Mucosal strips and also increased ATP release up to 30-fold, without evidence of desensitization. Amiloride (0.3 μM) reduced the acid-evoked ATP release by approximately 70%, while capsazepine (10 μM) reduced acid-evoked ATP release at pH 6.0 and pH 5.6 (by 68% and 61%, respectively). Capsaicin (0.1-10 μM) was ineffective in causing ATP release, and also failed to contract porcine Mucosal or detrusor strips. Gene expression for ASIC1, ASIC2, ASIC3 and TRPV1 was seen in the lateral wall, dome, trigone and neck of both detrusor and Mucosa. In conclusion, stretch and acid induce ATP release in the porcine Bladder Mucosa, but capsaicin is ineffective. The pig Bladder is a well-known model for the human Bladder, however these data suggest that it should be used with caution, particularly for TRPV1 related studies.
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release of atp from rat urinary Bladder Mucosa role of acid vanilloids and stretch
British Journal of Pharmacology, 2009Co-Authors: Prajni Sadananda, Kylie J Mansfield, Lu Liu, Fei Shang, Elizabeth BurcherAbstract:Background and purpose: ATP, released from urothelial cells, modulates afferent nerve firing from the urinary Bladder. Here, we have characterized ATP release from the rat Bladder Mucosa in response to acid, capsaicin, electrical field stimulation (EFS) and stretch, using agonists and antagonists at transient receptor potential vanilloid receptor 1 (TRPV1) and acid-sensing ion channels (ASICs). Experimental approach: Rat Mucosal strips (containing urothelium and lamina propria) in Perspex microbaths were superfused with Krebs solution. ATP was measured after exposure of matched strips to acid (pH 6.6–5.0), capsaicin (0.1–10 µM), EFS or stretch (150% of original length). Key results: Median basal ATP release was 3.46 nmol·g−1. The Mucosal strips responded to stimuli with potency order (median, IQR): acid (pH 5.6–6.0) 286 (103–555) > 10 µM capsaicin 188 (117–431) > 10 Hz EFS 63.0 (13.3–96.4) > stretch 24.4 (6.73–55.1) nmol ATP g−1. ATP release in response to acid was pH dependent (P < 0.05). Responses to capsaicin did not desensitize nor were they concentration dependent. TRPV1 antagonist, capsazepine (10 µM) abolished capsaicin-evoked ATP release, and reduced acid-evoked (pH 6.5) release to 30% (P < 0.001). The ASIC channel antagonists gadolinium (0.1 mM) and amiloride (0.3 µM) reduced (P < 0.05) the acid-evoked (pH 6.5) release to 40 and 6.5% respectively. ASIC (ASIC1, ASIC2a, ASIC2b, ASIC3) and two TRPV1 gene products were detected in Mucosal and detrusor extracts. Conclusions and implications: Capsaicin (at TRPV1) and acid (at both TRPV1 and ASIC) induce ATP release from the rat Bladder Mucosa. This ATP appears to be principally of urothelial origin. This study highlights the importance of ATP and acid as signalling molecules in modulating Bladder function.
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Acid, but not capsaicin, is an effective stimulus for ATP release in the porcine Bladder Mucosa
Neurourology and Urodynamics, 2009Co-Authors: Prajni Sandananda, Kylie J Mansfield, Elizabeth BurcherAbstract:Hypothesis / aims of study: Urothelial ATP release is thought to play an important role in Bladder afferent signaling via activation of purinergic receptors on suburothelial afferent nerves. Stretch of the Bladder Mucosa is a well documented stimulus for ATP release in several species, including the pig [1]. In addition, in mouse Bladder, capsaicin is also an effective stimulus for ATP release, acting via stimulation of vanilloid (TRPV1) receptors [2]. While acid is an agonist at the TRPV1 receptor, specialized acid sensing ion channels (ASICs) are also present in several organ systems [3]. Our aim was to characterize the ATP release from pig Bladder Mucosa in response to stretch, acid and capsaicin.
Shusen Xie - One of the best experts on this subject based on the ideXlab platform.
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Multiphoton microscopy of unstained Bladder Mucosa based on two‐photon excited autofluorescence and second harmonic generation
Laser Physics Letters, 2009Co-Authors: Shuangmu Zhuo, J.x. Chen, Tianshu Luo, Xingshan Jiang, Shusen XieAbstract:This work demonstrates the use of multiphoton microscopy based on two-photon excited autofluorescence and second harmonic generation in obtaining layered-resolved biomorphologic and biochemical features of the Bladder Mucosa.
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multiphoton microscopy of unstained Bladder Mucosa based on two photon excited autofluorescence and second harmonic generation
Laser Physics Letters, 2009Co-Authors: Shuangmu Zhuo, J.x. Chen, Tianshu Luo, Xingshan Jiang, Shusen XieAbstract:This work demonstrates the use of multiphoton microscopy based on two-photon excited autofluorescence and second harmonic generation in obtaining layered-resolved biomorphologic and biochemical features of the Bladder Mucosa.
Carla Daisy Costa Albuquerque - One of the best experts on this subject based on the ideXlab platform.
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treatment of vesicovaginal fistula by Bladder Mucosa autograft technique
Journal of The American College of Surgeons, 1998Co-Authors: Frederico Teixeira Brandt, Felipe Rinald Lorenzato, Carla Daisy Costa AlbuquerqueAbstract:Abstract Objectives: We evaluated the efficacy of the Bladder Mucosa autograft technique in the treatment of vesicovaginal fistula. Study Design: During 15 years, 80 women with vesicovaginal fistula after gynecologic surgery for benign disease were treated by interposition of a Bladder Mucosa autograft in the fistulous tract within 1–3 months of its genesis. Results: The success rate of this surgical intervention was 96.3%. There was no late failure. Conclusions: Bladder Mucosa autograft interposition seems to be an excellent therapy for closing vesicovaginal fistulas. It appears to be more physiologic and easier to perform when compared with other transabdominal techniques. Early intervention proved to be efficacious and to improve the woman's quality of life.
R. Bankole - One of the best experts on this subject based on the ideXlab platform.
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Urethral stricture in children : treatment by urethroplasty with Bladder Mucosa graft
The Journal of urology, 1992Co-Authors: G. Monfort, D. Bretheau, V. Di Benedetto, R. BankoleAbstract:AbstractUrethral strictures in children, which are not frequent, often require urethroplasty when dilations and/or urethrotomies have failed. A Bladder Mucosa graft was used successfully for urethral reconstruction to treat posterior hypospadias. We describe our experience with a Bladder Mucosa graft during urethroplasty for acquired urethral strictures in 8 children. Urethral strictures secondary to the treatment of hypospadias were excluded. Bladder Mucosa was used successfully as an onlay or patch graft urethroplasty in 7 patients. One patient had a tubularized graft with secondary stenosis treated successfully by dilation.
Shuangmu Zhuo - One of the best experts on this subject based on the ideXlab platform.
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Multiphoton microscopy of unstained Bladder Mucosa based on two‐photon excited autofluorescence and second harmonic generation
Laser Physics Letters, 2009Co-Authors: Shuangmu Zhuo, J.x. Chen, Tianshu Luo, Xingshan Jiang, Shusen XieAbstract:This work demonstrates the use of multiphoton microscopy based on two-photon excited autofluorescence and second harmonic generation in obtaining layered-resolved biomorphologic and biochemical features of the Bladder Mucosa.
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multiphoton microscopy of unstained Bladder Mucosa based on two photon excited autofluorescence and second harmonic generation
Laser Physics Letters, 2009Co-Authors: Shuangmu Zhuo, J.x. Chen, Tianshu Luo, Xingshan Jiang, Shusen XieAbstract:This work demonstrates the use of multiphoton microscopy based on two-photon excited autofluorescence and second harmonic generation in obtaining layered-resolved biomorphologic and biochemical features of the Bladder Mucosa.