The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Olav Andreas Aarstad - One of the best experts on this subject based on the ideXlab platform.
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Alginate sequencing: Block Distribution in alginates and its impact on macroscopic properties
2013Co-Authors: Olav Andreas AarstadAbstract:Alginate sequencing : Block Distribution in alginates and its impact on macroscopic properties
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alginate sequencing an analysis of Block Distribution in alginates using specific alginate degrading enzymes
Biomacromolecules, 2012Co-Authors: Olav Andreas Aarstad, Anne Tondervik, Havard Sletta, Gudmund SkjakbraekAbstract:Distribution and proportion of β-d-mannuronic and α-l-guluronic acid in alginates are important for understanding the chemical-physical properties of the polymer. The present state of art methods, which is based on NMR, provides a statistical description of alginates. In this work, a method was developed that also gives information of the Distribution of Block lengths of each of the three Block types (M, G, and MG Blocks). This was achieved using a combination of alginate lyases with different substrate specificities, including a novel lyase that specifically cleaves diguluronic acid linkages. Reaction products and isolated fragments of alginates degraded with these lyases were subsequently analyzed with 1H NMR, HPAEC-PAD, and SEC-MALLS. The method was applied on three seaweed alginates with large differences in sequence parameters (FG = 0.32 to 0.67). All samples contained considerable amounts of extremely long G Blocks (DP > 100). The finding of long M Blocks (DP ≥ 90) suggests that also algal epimerase...
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Alginate sequencing: an analysis of Block Distribution in alginates using specific alginate degrading enzymes.
Biomacromolecules, 2011Co-Authors: Olav Andreas Aarstad, Anne Tondervik, Havard Sletta, Gudmund Skjåk-brækAbstract:Distribution and proportion of β-d-mannuronic and α-l-guluronic acid in alginates are important for understanding the chemical-physical properties of the polymer. The present state of art methods, ...
Gudmund Skjakbraek - One of the best experts on this subject based on the ideXlab platform.
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alginate sequencing an analysis of Block Distribution in alginates using specific alginate degrading enzymes
Biomacromolecules, 2012Co-Authors: Olav Andreas Aarstad, Anne Tondervik, Havard Sletta, Gudmund SkjakbraekAbstract:Distribution and proportion of β-d-mannuronic and α-l-guluronic acid in alginates are important for understanding the chemical-physical properties of the polymer. The present state of art methods, which is based on NMR, provides a statistical description of alginates. In this work, a method was developed that also gives information of the Distribution of Block lengths of each of the three Block types (M, G, and MG Blocks). This was achieved using a combination of alginate lyases with different substrate specificities, including a novel lyase that specifically cleaves diguluronic acid linkages. Reaction products and isolated fragments of alginates degraded with these lyases were subsequently analyzed with 1H NMR, HPAEC-PAD, and SEC-MALLS. The method was applied on three seaweed alginates with large differences in sequence parameters (FG = 0.32 to 0.67). All samples contained considerable amounts of extremely long G Blocks (DP > 100). The finding of long M Blocks (DP ≥ 90) suggests that also algal epimerase...
Anne Tondervik - One of the best experts on this subject based on the ideXlab platform.
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alginate sequencing an analysis of Block Distribution in alginates using specific alginate degrading enzymes
Biomacromolecules, 2012Co-Authors: Olav Andreas Aarstad, Anne Tondervik, Havard Sletta, Gudmund SkjakbraekAbstract:Distribution and proportion of β-d-mannuronic and α-l-guluronic acid in alginates are important for understanding the chemical-physical properties of the polymer. The present state of art methods, which is based on NMR, provides a statistical description of alginates. In this work, a method was developed that also gives information of the Distribution of Block lengths of each of the three Block types (M, G, and MG Blocks). This was achieved using a combination of alginate lyases with different substrate specificities, including a novel lyase that specifically cleaves diguluronic acid linkages. Reaction products and isolated fragments of alginates degraded with these lyases were subsequently analyzed with 1H NMR, HPAEC-PAD, and SEC-MALLS. The method was applied on three seaweed alginates with large differences in sequence parameters (FG = 0.32 to 0.67). All samples contained considerable amounts of extremely long G Blocks (DP > 100). The finding of long M Blocks (DP ≥ 90) suggests that also algal epimerase...
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Alginate sequencing: an analysis of Block Distribution in alginates using specific alginate degrading enzymes.
Biomacromolecules, 2011Co-Authors: Olav Andreas Aarstad, Anne Tondervik, Havard Sletta, Gudmund Skjåk-brækAbstract:Distribution and proportion of β-d-mannuronic and α-l-guluronic acid in alginates are important for understanding the chemical-physical properties of the polymer. The present state of art methods, ...
Havard Sletta - One of the best experts on this subject based on the ideXlab platform.
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alginate sequencing an analysis of Block Distribution in alginates using specific alginate degrading enzymes
Biomacromolecules, 2012Co-Authors: Olav Andreas Aarstad, Anne Tondervik, Havard Sletta, Gudmund SkjakbraekAbstract:Distribution and proportion of β-d-mannuronic and α-l-guluronic acid in alginates are important for understanding the chemical-physical properties of the polymer. The present state of art methods, which is based on NMR, provides a statistical description of alginates. In this work, a method was developed that also gives information of the Distribution of Block lengths of each of the three Block types (M, G, and MG Blocks). This was achieved using a combination of alginate lyases with different substrate specificities, including a novel lyase that specifically cleaves diguluronic acid linkages. Reaction products and isolated fragments of alginates degraded with these lyases were subsequently analyzed with 1H NMR, HPAEC-PAD, and SEC-MALLS. The method was applied on three seaweed alginates with large differences in sequence parameters (FG = 0.32 to 0.67). All samples contained considerable amounts of extremely long G Blocks (DP > 100). The finding of long M Blocks (DP ≥ 90) suggests that also algal epimerase...
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Alginate sequencing: an analysis of Block Distribution in alginates using specific alginate degrading enzymes.
Biomacromolecules, 2011Co-Authors: Olav Andreas Aarstad, Anne Tondervik, Havard Sletta, Gudmund Skjåk-brækAbstract:Distribution and proportion of β-d-mannuronic and α-l-guluronic acid in alginates are important for understanding the chemical-physical properties of the polymer. The present state of art methods, ...
Gudmund Skjåk-bræk - One of the best experts on this subject based on the ideXlab platform.
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Alginate sequencing: an analysis of Block Distribution in alginates using specific alginate degrading enzymes.
Biomacromolecules, 2011Co-Authors: Olav Andreas Aarstad, Anne Tondervik, Havard Sletta, Gudmund Skjåk-brækAbstract:Distribution and proportion of β-d-mannuronic and α-l-guluronic acid in alginates are important for understanding the chemical-physical properties of the polymer. The present state of art methods, ...