The Experts below are selected from a list of 82899 Experts worldwide ranked by ideXlab platform

Raquel Riquelme - One of the best experts on this subject based on the ideXlab platform.

  • generation of mouse zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host pathogen interaction studies
    Disease Models & Mechanisms, 2018
    Co-Authors: Margarita Paradakusz, Cristina Penaranda, Elliott J Hagedorn, Anne E Clatworthy, Anil V Nair, Jonathan E Henninger, Christoph M Ernst, Brian Li, Raquel Riquelme
    Abstract:

    ABSTRACT Xenografts of the hematopoietic system are extremely useful as disease models and for translational research. Zebrafish xenografts have been widely used to monitor Blood Cancer Cell dissemination and homing due to the optical clarity of embryos and larvae, which allow unrestricted in vivo visualization of migratory events. Here, we have developed a xenotransplantation technique that transiently generates hundreds of hematopoietic tissue chimeric embryos by transplanting murine bone marrow Cells into zebrafish blastulae. In contrast to previous methods, this procedure allows mammalian Cell integration into the fish developmental hematopoietic program, which results in chimeric animals containing distinct phenotypes of murine Blood Cells in both circulation and the hematopoietic niche. Murine Cells in chimeric animals express antigens related to (i) hematopoietic stem and progenitor Cells, (ii) active Cell proliferation and (iii) myeloid Cell lineages. We verified the utility of this method by monitoring zebrafish chimeras during development using in vivo non-invasive imaging to show novel murine Cell behaviors, such as homing to primitive and definitive hematopoietic tissues, dynamic hematopoietic Cell and hematopoietic niche interactions, and response to bacterial infection. Overall, transplantation into the zebrafish blastula provides a useful method that simplifies the generation of numerous chimeric animals and expands the range of murine Cell behaviors that can be studied in zebrafish chimeras. In addition, integration of murine Cells into the host hematopoietic system during development suggests highly conserved molecular mechanisms of hematopoiesis between zebrafish and mammals. This article has an associated First Person interview with the first author of the paper.

  • generation of mouse zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host pathogen interaction studies
    bioRxiv, 2017
    Co-Authors: Margarita Paradakusz, Cristina Penaranda, Elliott J Hagedorn, Anne E Clatworthy, Anil V Nair, Jonathan E Henninger, Christoph M Ernst, Brian Li, Raquel Riquelme, Humberto Jijon
    Abstract:

    ABSTRACT Xenografts of the hematopoietic system are extremely useful as disease models and for translational research. Zebrafish xenografts have been widely used to monitor Blood Cancer Cell dissemination and homing due to the optical clarity of embryos and larvae, which allow unrestricted in vivo visualization of migratory events. To broaden the scope of xenotransplantation studies in zebrafish, we have developed a technique that transiently generates hematopoietic tissue chimeras by transplanting murine bone marrow Cells into zebrafish blastulae. This procedure leads to mammalian Cell integration into the fish developmental hematopoietic program. Monitoring zebrafish chimeras at different time points post fertilization using in vivo time-lapse and confocal imaging showed murine Cell co-localization with developing primitive and definitive hematopoietic tissues, intravasation into fish circulation, and dynamic hematopoietic Cell-vascular endothelial and hematopoietic Cell-niche interactions. Immunohistochemistry assays performed in chimeric animals showed that, after engraftment, murine Cells expressed antigens related to i) hematopoietic stem and progenitor Cells, ii) active Cell proliferation, and iii) myeloid Cell lineages. Lastly, xenografted zebrafish larvae infected with Klebsiella pneumoniae showed murine immune Cells trafficking to bacterial foci and interacting with bacterial Cells. Overall, these results show that mammalian bone marrow Cells xenografted in zebrafish integrate into the host hematopoietic system revealing highly conserved molecular mechanisms of hematopoiesis between zebrafish and mammals. In addition, this procedure introduces a useful and simple method that improves and broadens the scope of hematopoietic tissue xenotransplantation studies in zebrafish.

Anne E Clatworthy - One of the best experts on this subject based on the ideXlab platform.

  • generation of mouse zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host pathogen interaction studies
    Disease Models & Mechanisms, 2018
    Co-Authors: Margarita Paradakusz, Cristina Penaranda, Elliott J Hagedorn, Anne E Clatworthy, Anil V Nair, Jonathan E Henninger, Christoph M Ernst, Brian Li, Raquel Riquelme
    Abstract:

    ABSTRACT Xenografts of the hematopoietic system are extremely useful as disease models and for translational research. Zebrafish xenografts have been widely used to monitor Blood Cancer Cell dissemination and homing due to the optical clarity of embryos and larvae, which allow unrestricted in vivo visualization of migratory events. Here, we have developed a xenotransplantation technique that transiently generates hundreds of hematopoietic tissue chimeric embryos by transplanting murine bone marrow Cells into zebrafish blastulae. In contrast to previous methods, this procedure allows mammalian Cell integration into the fish developmental hematopoietic program, which results in chimeric animals containing distinct phenotypes of murine Blood Cells in both circulation and the hematopoietic niche. Murine Cells in chimeric animals express antigens related to (i) hematopoietic stem and progenitor Cells, (ii) active Cell proliferation and (iii) myeloid Cell lineages. We verified the utility of this method by monitoring zebrafish chimeras during development using in vivo non-invasive imaging to show novel murine Cell behaviors, such as homing to primitive and definitive hematopoietic tissues, dynamic hematopoietic Cell and hematopoietic niche interactions, and response to bacterial infection. Overall, transplantation into the zebrafish blastula provides a useful method that simplifies the generation of numerous chimeric animals and expands the range of murine Cell behaviors that can be studied in zebrafish chimeras. In addition, integration of murine Cells into the host hematopoietic system during development suggests highly conserved molecular mechanisms of hematopoiesis between zebrafish and mammals. This article has an associated First Person interview with the first author of the paper.

  • generation of mouse zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host pathogen interaction studies
    bioRxiv, 2017
    Co-Authors: Margarita Paradakusz, Cristina Penaranda, Elliott J Hagedorn, Anne E Clatworthy, Anil V Nair, Jonathan E Henninger, Christoph M Ernst, Brian Li, Raquel Riquelme, Humberto Jijon
    Abstract:

    ABSTRACT Xenografts of the hematopoietic system are extremely useful as disease models and for translational research. Zebrafish xenografts have been widely used to monitor Blood Cancer Cell dissemination and homing due to the optical clarity of embryos and larvae, which allow unrestricted in vivo visualization of migratory events. To broaden the scope of xenotransplantation studies in zebrafish, we have developed a technique that transiently generates hematopoietic tissue chimeras by transplanting murine bone marrow Cells into zebrafish blastulae. This procedure leads to mammalian Cell integration into the fish developmental hematopoietic program. Monitoring zebrafish chimeras at different time points post fertilization using in vivo time-lapse and confocal imaging showed murine Cell co-localization with developing primitive and definitive hematopoietic tissues, intravasation into fish circulation, and dynamic hematopoietic Cell-vascular endothelial and hematopoietic Cell-niche interactions. Immunohistochemistry assays performed in chimeric animals showed that, after engraftment, murine Cells expressed antigens related to i) hematopoietic stem and progenitor Cells, ii) active Cell proliferation, and iii) myeloid Cell lineages. Lastly, xenografted zebrafish larvae infected with Klebsiella pneumoniae showed murine immune Cells trafficking to bacterial foci and interacting with bacterial Cells. Overall, these results show that mammalian bone marrow Cells xenografted in zebrafish integrate into the host hematopoietic system revealing highly conserved molecular mechanisms of hematopoiesis between zebrafish and mammals. In addition, this procedure introduces a useful and simple method that improves and broadens the scope of hematopoietic tissue xenotransplantation studies in zebrafish.

Phillip H Koeffler - One of the best experts on this subject based on the ideXlab platform.

  • profiling the b t Cell receptor repertoire of lymphocyte derived Cell lines
    BMC Cancer, 2018
    Co-Authors: Lingwen Ding, Wenwen Chien, Jinfen Xiao, Liang Xu, Michael Lill, Anand Mayakonda, Henry Yang, Phillip H Koeffler
    Abstract:

    Clonal VDJ rearrangement of B/T Cell receptors (B/TCRs) occurring during B/T lymphocyte development has been used as a marker to track the clonality of B/T Cell populations. We systematically profiled the B/T Cell receptor repertoire of 936 Cancer Cell lines across a variety of Cancer types as well as 462 Epstein-Barr Virus (EBV) transformed normal B lymphocyte lines using RNA sequencing data. Rearranged B/TCRs were readily detected in Cell lines derived from lymphocytes, and subclonality or potential biclonality were found in a number of Blood Cancer Cell lines. Clonal BCR/TCR rearrangements were detected in several blast phase CML lines and unexpectedly, one gastric Cancer Cell line (KE-97), reflecting a lymphoid origin of these Cells. Notably, clonality was highly prevalent in EBV transformed B lymphocytes, suggesting either transformation only occurred in a few B Cells or those with a growth advantage dominated the transformed population through clonal evolution. Our analysis reveals the complexity and heterogeneity of the BCR/TCR rearrangement repertoire and provides a unique insight into the clonality of lymphocyte derived Cell lines.

Margarita Paradakusz - One of the best experts on this subject based on the ideXlab platform.

  • generation of mouse zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host pathogen interaction studies
    Disease Models & Mechanisms, 2018
    Co-Authors: Margarita Paradakusz, Cristina Penaranda, Elliott J Hagedorn, Anne E Clatworthy, Anil V Nair, Jonathan E Henninger, Christoph M Ernst, Brian Li, Raquel Riquelme
    Abstract:

    ABSTRACT Xenografts of the hematopoietic system are extremely useful as disease models and for translational research. Zebrafish xenografts have been widely used to monitor Blood Cancer Cell dissemination and homing due to the optical clarity of embryos and larvae, which allow unrestricted in vivo visualization of migratory events. Here, we have developed a xenotransplantation technique that transiently generates hundreds of hematopoietic tissue chimeric embryos by transplanting murine bone marrow Cells into zebrafish blastulae. In contrast to previous methods, this procedure allows mammalian Cell integration into the fish developmental hematopoietic program, which results in chimeric animals containing distinct phenotypes of murine Blood Cells in both circulation and the hematopoietic niche. Murine Cells in chimeric animals express antigens related to (i) hematopoietic stem and progenitor Cells, (ii) active Cell proliferation and (iii) myeloid Cell lineages. We verified the utility of this method by monitoring zebrafish chimeras during development using in vivo non-invasive imaging to show novel murine Cell behaviors, such as homing to primitive and definitive hematopoietic tissues, dynamic hematopoietic Cell and hematopoietic niche interactions, and response to bacterial infection. Overall, transplantation into the zebrafish blastula provides a useful method that simplifies the generation of numerous chimeric animals and expands the range of murine Cell behaviors that can be studied in zebrafish chimeras. In addition, integration of murine Cells into the host hematopoietic system during development suggests highly conserved molecular mechanisms of hematopoiesis between zebrafish and mammals. This article has an associated First Person interview with the first author of the paper.

  • generation of mouse zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host pathogen interaction studies
    bioRxiv, 2017
    Co-Authors: Margarita Paradakusz, Cristina Penaranda, Elliott J Hagedorn, Anne E Clatworthy, Anil V Nair, Jonathan E Henninger, Christoph M Ernst, Brian Li, Raquel Riquelme, Humberto Jijon
    Abstract:

    ABSTRACT Xenografts of the hematopoietic system are extremely useful as disease models and for translational research. Zebrafish xenografts have been widely used to monitor Blood Cancer Cell dissemination and homing due to the optical clarity of embryos and larvae, which allow unrestricted in vivo visualization of migratory events. To broaden the scope of xenotransplantation studies in zebrafish, we have developed a technique that transiently generates hematopoietic tissue chimeras by transplanting murine bone marrow Cells into zebrafish blastulae. This procedure leads to mammalian Cell integration into the fish developmental hematopoietic program. Monitoring zebrafish chimeras at different time points post fertilization using in vivo time-lapse and confocal imaging showed murine Cell co-localization with developing primitive and definitive hematopoietic tissues, intravasation into fish circulation, and dynamic hematopoietic Cell-vascular endothelial and hematopoietic Cell-niche interactions. Immunohistochemistry assays performed in chimeric animals showed that, after engraftment, murine Cells expressed antigens related to i) hematopoietic stem and progenitor Cells, ii) active Cell proliferation, and iii) myeloid Cell lineages. Lastly, xenografted zebrafish larvae infected with Klebsiella pneumoniae showed murine immune Cells trafficking to bacterial foci and interacting with bacterial Cells. Overall, these results show that mammalian bone marrow Cells xenografted in zebrafish integrate into the host hematopoietic system revealing highly conserved molecular mechanisms of hematopoiesis between zebrafish and mammals. In addition, this procedure introduces a useful and simple method that improves and broadens the scope of hematopoietic tissue xenotransplantation studies in zebrafish.

Anil V Nair - One of the best experts on this subject based on the ideXlab platform.

  • generation of mouse zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host pathogen interaction studies
    Disease Models & Mechanisms, 2018
    Co-Authors: Margarita Paradakusz, Cristina Penaranda, Elliott J Hagedorn, Anne E Clatworthy, Anil V Nair, Jonathan E Henninger, Christoph M Ernst, Brian Li, Raquel Riquelme
    Abstract:

    ABSTRACT Xenografts of the hematopoietic system are extremely useful as disease models and for translational research. Zebrafish xenografts have been widely used to monitor Blood Cancer Cell dissemination and homing due to the optical clarity of embryos and larvae, which allow unrestricted in vivo visualization of migratory events. Here, we have developed a xenotransplantation technique that transiently generates hundreds of hematopoietic tissue chimeric embryos by transplanting murine bone marrow Cells into zebrafish blastulae. In contrast to previous methods, this procedure allows mammalian Cell integration into the fish developmental hematopoietic program, which results in chimeric animals containing distinct phenotypes of murine Blood Cells in both circulation and the hematopoietic niche. Murine Cells in chimeric animals express antigens related to (i) hematopoietic stem and progenitor Cells, (ii) active Cell proliferation and (iii) myeloid Cell lineages. We verified the utility of this method by monitoring zebrafish chimeras during development using in vivo non-invasive imaging to show novel murine Cell behaviors, such as homing to primitive and definitive hematopoietic tissues, dynamic hematopoietic Cell and hematopoietic niche interactions, and response to bacterial infection. Overall, transplantation into the zebrafish blastula provides a useful method that simplifies the generation of numerous chimeric animals and expands the range of murine Cell behaviors that can be studied in zebrafish chimeras. In addition, integration of murine Cells into the host hematopoietic system during development suggests highly conserved molecular mechanisms of hematopoiesis between zebrafish and mammals. This article has an associated First Person interview with the first author of the paper.

  • generation of mouse zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host pathogen interaction studies
    bioRxiv, 2017
    Co-Authors: Margarita Paradakusz, Cristina Penaranda, Elliott J Hagedorn, Anne E Clatworthy, Anil V Nair, Jonathan E Henninger, Christoph M Ernst, Brian Li, Raquel Riquelme, Humberto Jijon
    Abstract:

    ABSTRACT Xenografts of the hematopoietic system are extremely useful as disease models and for translational research. Zebrafish xenografts have been widely used to monitor Blood Cancer Cell dissemination and homing due to the optical clarity of embryos and larvae, which allow unrestricted in vivo visualization of migratory events. To broaden the scope of xenotransplantation studies in zebrafish, we have developed a technique that transiently generates hematopoietic tissue chimeras by transplanting murine bone marrow Cells into zebrafish blastulae. This procedure leads to mammalian Cell integration into the fish developmental hematopoietic program. Monitoring zebrafish chimeras at different time points post fertilization using in vivo time-lapse and confocal imaging showed murine Cell co-localization with developing primitive and definitive hematopoietic tissues, intravasation into fish circulation, and dynamic hematopoietic Cell-vascular endothelial and hematopoietic Cell-niche interactions. Immunohistochemistry assays performed in chimeric animals showed that, after engraftment, murine Cells expressed antigens related to i) hematopoietic stem and progenitor Cells, ii) active Cell proliferation, and iii) myeloid Cell lineages. Lastly, xenografted zebrafish larvae infected with Klebsiella pneumoniae showed murine immune Cells trafficking to bacterial foci and interacting with bacterial Cells. Overall, these results show that mammalian bone marrow Cells xenografted in zebrafish integrate into the host hematopoietic system revealing highly conserved molecular mechanisms of hematopoiesis between zebrafish and mammals. In addition, this procedure introduces a useful and simple method that improves and broadens the scope of hematopoietic tissue xenotransplantation studies in zebrafish.