The Experts below are selected from a list of 261 Experts worldwide ranked by ideXlab platform

Philippe Parola - One of the best experts on this subject based on the ideXlab platform.

  • Use of MALDI-TOF MS for the Identification of Chad Mosquitoes and the Origin of Their Blood Meal
    American Journal of Tropical Medicine and Hygiene, 2019
    Co-Authors: Adama Zan Diarra, Maureen Laroche, Franck Berger, Philippe Parola
    Abstract:

    Matrix-assisted desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a clinical microbiology tool for the systematic identification of microorganisms. It has recently been presented as an innovative tool for the rapid and accurate identification of mosquitoes and their Blood Meal. To evaluate the capacity of this tool to identify mosquitoes collected in a tropical environment and preserved with silica gel, we analyzed 188 mosquitoes of different species collected in Chad, which were preserved with silica gel for 2 months. The MALDI-TOF MS analysis correctly identified 96% of the mosquitoes and 37.5% of their Blood Meals. Using MALDI-TOF MS and molecular biology, eight mosquito species were identified, including Anopheles gambiae s.l., Anopheles rufipes, Culex quinquefasciatus, Culex neavei, Culex pipiens, Culex perexiguus, Culex rima, and Culex watti. Blood Meal identification revealed that mosquitoes fed mainly on humans, birds, and cows. Matrix-assisted desorption/ionization time-of-flight mass spectrometry appears to be a promising, fast, and reliable tool to identify mosquitoes and the origin of their Blood Meal for samples stored with silica gel.

  • Blood Meal identification in the cryptic species Anopheles gambiae and Anopheles coluzzii using MALDI-TOF MS
    Parasite, 2018
    Co-Authors: Fatalmoudou Tandina, Maureen Laroche, Ogobara K Doumbo, Bernard Davoust, Philippe Parola
    Abstract:

    Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has recently emerged in entomology as a technique to identify arthropods and their Blood Meal source. In this study, female Anopheles gambiae were fed on five host Blood sources: ocelot (Leopardus pardalis), binturong (Arctictis binturong), springbok (Antidorcas marsupialis), jaguar (Panthera onca) and Hamadryas baboon (Papio hamadryas), while Anopheles coluzzii were fed on three hosts: dromedary (Camelus dromedarius), Barbary sheep (Ammotragus lervia) and pig (Sus scrofa). We obtained the MS spectra from 240 engorged mosquito abdomens and selected high quality ones from 72 mosquito abdomens to upgrade our home-made database. We excluded from the analysis any spectra of low quality (n = 80), and the remaining 88 specimens were subjected to a blind test analysis against the home-made database. We obtained 100% correct identification of the Blood Meal source for the specimens collected, 1, 12 and 24 h post-feeding, whereas for the specimens collected 36 h post-feeding, the correct identification rate decreased dramatically. We confirm here that MALDI-TOF MS can be used to identify the Blood Meal origin of freshly engorged mosquitoes, which opens new perspectives for further studies, including the impact of the mosquito species on Blood Meal identification.

  • identification of Blood Meal sources in the main african malaria mosquito vector by maldi tof ms
    Malaria Journal, 2016
    Co-Authors: Sirama Niare, Constentin Dieme, Ogobara K Doumbo, Jean Michel Berenger, Philippe Parola, Didier Raoult, Lionel Almeras
    Abstract:

    Background The identification of Blood Meal sources in malaria vectors is critical to better understanding host/vector interactions and malaria epidemiology and control. Currently, the identification of mosquito Blood Meal origins is based on time-consuming and costly techniques such as precipitin tests, ELISA and molecular tools. Although these tools have been validated to identify the Blood Meal and trophic preferences of female Anopheles mosquitoes, they present several limitations. Recently, matrix-assisted, laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was successfully used as a quick and accurate tool for arthropod identification, including mosquitoes. The aim of the present work was to test whether MALDI-TOF MS could also be applied to identification of Blood Meal sources from engorged mosquitoes.

  • Identification of Blood Meal sources in the main African malaria mosquito vector by MALDI-TOF MS
    Malaria Journal, 2016
    Co-Authors: Sirama Niare, Constentin Dieme, Ogobara K Doumbo, Jean Michel Berenger, Philippe Parola, Lionel Almeras
    Abstract:

    Background: The identification of Blood Meal sources in malaria vectors is critical to better understanding host/vector interactions and malaria epidemiology and control. Currently, the identification of mosquito Blood Meal origins is based on time-consuming and costly techniques such as precipitin tests, ELISA and molecular tools. Although these tools have been validated to identify the Blood Meal and trophic preferences of female Anopheles mosquitoes, they present several limitations. Recently, matrix-assisted, laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was successfully used as a quick and accurate tool for arthropod identification, including mosquitoes. The aim of the present work was to test whether MALDI-TOF MS could also be applied to identification of Blood Meal sources from engorged mosquitoes. Methods: Abdomen proteins extracted from Anopheles gambiae (stricto sensu, S molecular form) that were either unengorged or artificially engorged on seven distinct types of vertebrate Blood (human, horse, sheep, rabbit, mouse, rat, dog) were submitted for MALDI-TOF MS. Results: The comparison of mass spectrometry (MS) spectra from mosquito abdomens collected 1 h post-feeding, were able to discriminate Blood Meal origins. Moreover, using Aedes albopictus specimens, abdominal protein MS spectra from engorged mosquitoes were found specific to host Blood source and independent of the mosquito species. A sequential analysis revealed stability of mosquito abdominal protein spectra up to 24 h post-feeding. Conclusions: These results indicate that MALDI-TOF MS could determine feeding patterns of freshly engorged mosquitoes up to 24 h post-Blood Meal. The MALDI-TOF MS technique appears to be an efficient tool for large epidemiological surveillance of vector-borne diseases and outbreak source identification.

  • Identification of Blood Meal sources in the main African malaria mosquito vector
    2016
    Co-Authors: Sirama Niare, Constentin Dieme, Ogobara K Doumbo, Jean Michel Berenger, Philippe Parola, Didier Raoult, Lionel Almeras
    Abstract:

    Background: The identification of Blood Meal sources in malaria vectors is critical to better understanding host/vec‑ tor interactions and malaria epidemiology and control. Currently, the identification of mosquito Blood Meal origins is based on time‑consuming and costly techniques such as precipitin tests, ELISA and molecular tools. Although these tools have been validated to identify the Blood Meal and trophic preferences of female Anopheles mosquitoes, they present several limitations. Recently, matrix‑assisted, laser desorption/ionization time‑of‑flight mass spectrometry (MALDI‑TOF MS) was successfully used as a quick and accurate tool for arthropod identification, including mosquitoes. The aim of the present work was to test whether MALDI‑TOF MS could also be applied to identification of Blood Meal sources from engorged mosquitoes. Methods: Abdomen proteins extracted from Anopheles gambiae (stricto sensu, S molecular form) that were either unengorged or artificially engorged on seven distinct types of vertebrate Blood (human, horse, sheep, rabbit, mouse, rat, dog) were submitted for MALDI‑TOF MS. Results: The comparison of mass spectrometry (MS) spectra from mosquito abdomens collected 1 h post‑feeding, were able to discriminate Blood Meal origins. Moreover, using Aedes albopictus specimens, abdominal protein MS spectra from engorged mosquitoes were found specific to host Blood source and independent of the mosquito spe ‑ cies. A sequential analysis revealed stability of mosquito abdominal protein spectra up to 24 h post‑feeding. Conclusions: These results indicate that MALDI‑TOF MS could determine feeding patterns of freshly engorged mos‑ quitoes up to 24 h post‑Blood Meal. The MALDI‑TOF MS technique appears to be an efficient tool for large epidemio ‑ logical surveillance of vector‑borne diseases and outbreak source identification.

Lionel Almeras - One of the best experts on this subject based on the ideXlab platform.

  • identification of Blood Meal sources in the main african malaria mosquito vector by maldi tof ms
    Malaria Journal, 2016
    Co-Authors: Sirama Niare, Constentin Dieme, Ogobara K Doumbo, Jean Michel Berenger, Philippe Parola, Didier Raoult, Lionel Almeras
    Abstract:

    Background The identification of Blood Meal sources in malaria vectors is critical to better understanding host/vector interactions and malaria epidemiology and control. Currently, the identification of mosquito Blood Meal origins is based on time-consuming and costly techniques such as precipitin tests, ELISA and molecular tools. Although these tools have been validated to identify the Blood Meal and trophic preferences of female Anopheles mosquitoes, they present several limitations. Recently, matrix-assisted, laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was successfully used as a quick and accurate tool for arthropod identification, including mosquitoes. The aim of the present work was to test whether MALDI-TOF MS could also be applied to identification of Blood Meal sources from engorged mosquitoes.

  • Identification of Blood Meal sources in the main African malaria mosquito vector by MALDI-TOF MS
    Malaria Journal, 2016
    Co-Authors: Sirama Niare, Constentin Dieme, Ogobara K Doumbo, Jean Michel Berenger, Philippe Parola, Lionel Almeras
    Abstract:

    Background: The identification of Blood Meal sources in malaria vectors is critical to better understanding host/vector interactions and malaria epidemiology and control. Currently, the identification of mosquito Blood Meal origins is based on time-consuming and costly techniques such as precipitin tests, ELISA and molecular tools. Although these tools have been validated to identify the Blood Meal and trophic preferences of female Anopheles mosquitoes, they present several limitations. Recently, matrix-assisted, laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was successfully used as a quick and accurate tool for arthropod identification, including mosquitoes. The aim of the present work was to test whether MALDI-TOF MS could also be applied to identification of Blood Meal sources from engorged mosquitoes. Methods: Abdomen proteins extracted from Anopheles gambiae (stricto sensu, S molecular form) that were either unengorged or artificially engorged on seven distinct types of vertebrate Blood (human, horse, sheep, rabbit, mouse, rat, dog) were submitted for MALDI-TOF MS. Results: The comparison of mass spectrometry (MS) spectra from mosquito abdomens collected 1 h post-feeding, were able to discriminate Blood Meal origins. Moreover, using Aedes albopictus specimens, abdominal protein MS spectra from engorged mosquitoes were found specific to host Blood source and independent of the mosquito species. A sequential analysis revealed stability of mosquito abdominal protein spectra up to 24 h post-feeding. Conclusions: These results indicate that MALDI-TOF MS could determine feeding patterns of freshly engorged mosquitoes up to 24 h post-Blood Meal. The MALDI-TOF MS technique appears to be an efficient tool for large epidemiological surveillance of vector-borne diseases and outbreak source identification.

  • Identification of Blood Meal sources in the main African malaria mosquito vector
    2016
    Co-Authors: Sirama Niare, Constentin Dieme, Ogobara K Doumbo, Jean Michel Berenger, Philippe Parola, Didier Raoult, Lionel Almeras
    Abstract:

    Background: The identification of Blood Meal sources in malaria vectors is critical to better understanding host/vec‑ tor interactions and malaria epidemiology and control. Currently, the identification of mosquito Blood Meal origins is based on time‑consuming and costly techniques such as precipitin tests, ELISA and molecular tools. Although these tools have been validated to identify the Blood Meal and trophic preferences of female Anopheles mosquitoes, they present several limitations. Recently, matrix‑assisted, laser desorption/ionization time‑of‑flight mass spectrometry (MALDI‑TOF MS) was successfully used as a quick and accurate tool for arthropod identification, including mosquitoes. The aim of the present work was to test whether MALDI‑TOF MS could also be applied to identification of Blood Meal sources from engorged mosquitoes. Methods: Abdomen proteins extracted from Anopheles gambiae (stricto sensu, S molecular form) that were either unengorged or artificially engorged on seven distinct types of vertebrate Blood (human, horse, sheep, rabbit, mouse, rat, dog) were submitted for MALDI‑TOF MS. Results: The comparison of mass spectrometry (MS) spectra from mosquito abdomens collected 1 h post‑feeding, were able to discriminate Blood Meal origins. Moreover, using Aedes albopictus specimens, abdominal protein MS spectra from engorged mosquitoes were found specific to host Blood source and independent of the mosquito spe ‑ cies. A sequential analysis revealed stability of mosquito abdominal protein spectra up to 24 h post‑feeding. Conclusions: These results indicate that MALDI‑TOF MS could determine feeding patterns of freshly engorged mos‑ quitoes up to 24 h post‑Blood Meal. The MALDI‑TOF MS technique appears to be an efficient tool for large epidemio ‑ logical surveillance of vector‑borne diseases and outbreak source identification.

Patrick C. H. Morel - One of the best experts on this subject based on the ideXlab platform.

  • Protein quality in Blood Meal
    Animal Feed Science and Technology, 1999
    Co-Authors: G. Pearson, Paul J. Moughan, G.z Dong, Patrick C. H. Morel
    Abstract:

    The laboratory rat was evaluated as a model animal for the growing pig for determining apparent amino acid digestibility in Blood Meal. Two test diets were formulated for rats and two for pigs. The sole source of dietary protein was Blood Meal and each pair of diets contained a low- and high-quality Blood Meal. Chromic oxide was included in the diets as an indigestible marker. On day 14 of the study ileal digesta were collected after slaughter of the rats and pigs, three and seven hours from the start of feeding, respectively. The apparent ileal digestibilities of amino acids were similar between the rats and pigs although a significant (p 

Sirama Niare - One of the best experts on this subject based on the ideXlab platform.

  • identification of Blood Meal sources in the main african malaria mosquito vector by maldi tof ms
    Malaria Journal, 2016
    Co-Authors: Sirama Niare, Constentin Dieme, Ogobara K Doumbo, Jean Michel Berenger, Philippe Parola, Didier Raoult, Lionel Almeras
    Abstract:

    Background The identification of Blood Meal sources in malaria vectors is critical to better understanding host/vector interactions and malaria epidemiology and control. Currently, the identification of mosquito Blood Meal origins is based on time-consuming and costly techniques such as precipitin tests, ELISA and molecular tools. Although these tools have been validated to identify the Blood Meal and trophic preferences of female Anopheles mosquitoes, they present several limitations. Recently, matrix-assisted, laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was successfully used as a quick and accurate tool for arthropod identification, including mosquitoes. The aim of the present work was to test whether MALDI-TOF MS could also be applied to identification of Blood Meal sources from engorged mosquitoes.

  • Identification of Blood Meal sources in the main African malaria mosquito vector by MALDI-TOF MS
    Malaria Journal, 2016
    Co-Authors: Sirama Niare, Constentin Dieme, Ogobara K Doumbo, Jean Michel Berenger, Philippe Parola, Lionel Almeras
    Abstract:

    Background: The identification of Blood Meal sources in malaria vectors is critical to better understanding host/vector interactions and malaria epidemiology and control. Currently, the identification of mosquito Blood Meal origins is based on time-consuming and costly techniques such as precipitin tests, ELISA and molecular tools. Although these tools have been validated to identify the Blood Meal and trophic preferences of female Anopheles mosquitoes, they present several limitations. Recently, matrix-assisted, laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was successfully used as a quick and accurate tool for arthropod identification, including mosquitoes. The aim of the present work was to test whether MALDI-TOF MS could also be applied to identification of Blood Meal sources from engorged mosquitoes. Methods: Abdomen proteins extracted from Anopheles gambiae (stricto sensu, S molecular form) that were either unengorged or artificially engorged on seven distinct types of vertebrate Blood (human, horse, sheep, rabbit, mouse, rat, dog) were submitted for MALDI-TOF MS. Results: The comparison of mass spectrometry (MS) spectra from mosquito abdomens collected 1 h post-feeding, were able to discriminate Blood Meal origins. Moreover, using Aedes albopictus specimens, abdominal protein MS spectra from engorged mosquitoes were found specific to host Blood source and independent of the mosquito species. A sequential analysis revealed stability of mosquito abdominal protein spectra up to 24 h post-feeding. Conclusions: These results indicate that MALDI-TOF MS could determine feeding patterns of freshly engorged mosquitoes up to 24 h post-Blood Meal. The MALDI-TOF MS technique appears to be an efficient tool for large epidemiological surveillance of vector-borne diseases and outbreak source identification.

  • Identification of Blood Meal sources in the main African malaria mosquito vector
    2016
    Co-Authors: Sirama Niare, Constentin Dieme, Ogobara K Doumbo, Jean Michel Berenger, Philippe Parola, Didier Raoult, Lionel Almeras
    Abstract:

    Background: The identification of Blood Meal sources in malaria vectors is critical to better understanding host/vec‑ tor interactions and malaria epidemiology and control. Currently, the identification of mosquito Blood Meal origins is based on time‑consuming and costly techniques such as precipitin tests, ELISA and molecular tools. Although these tools have been validated to identify the Blood Meal and trophic preferences of female Anopheles mosquitoes, they present several limitations. Recently, matrix‑assisted, laser desorption/ionization time‑of‑flight mass spectrometry (MALDI‑TOF MS) was successfully used as a quick and accurate tool for arthropod identification, including mosquitoes. The aim of the present work was to test whether MALDI‑TOF MS could also be applied to identification of Blood Meal sources from engorged mosquitoes. Methods: Abdomen proteins extracted from Anopheles gambiae (stricto sensu, S molecular form) that were either unengorged or artificially engorged on seven distinct types of vertebrate Blood (human, horse, sheep, rabbit, mouse, rat, dog) were submitted for MALDI‑TOF MS. Results: The comparison of mass spectrometry (MS) spectra from mosquito abdomens collected 1 h post‑feeding, were able to discriminate Blood Meal origins. Moreover, using Aedes albopictus specimens, abdominal protein MS spectra from engorged mosquitoes were found specific to host Blood source and independent of the mosquito spe ‑ cies. A sequential analysis revealed stability of mosquito abdominal protein spectra up to 24 h post‑feeding. Conclusions: These results indicate that MALDI‑TOF MS could determine feeding patterns of freshly engorged mos‑ quitoes up to 24 h post‑Blood Meal. The MALDI‑TOF MS technique appears to be an efficient tool for large epidemio ‑ logical surveillance of vector‑borne diseases and outbreak source identification.

G. Pearson - One of the best experts on this subject based on the ideXlab platform.

  • Protein quality in Blood Meal
    Animal Feed Science and Technology, 1999
    Co-Authors: G. Pearson, Paul J. Moughan, G.z Dong, Patrick C. H. Morel
    Abstract:

    The laboratory rat was evaluated as a model animal for the growing pig for determining apparent amino acid digestibility in Blood Meal. Two test diets were formulated for rats and two for pigs. The sole source of dietary protein was Blood Meal and each pair of diets contained a low- and high-quality Blood Meal. Chromic oxide was included in the diets as an indigestible marker. On day 14 of the study ileal digesta were collected after slaughter of the rats and pigs, three and seven hours from the start of feeding, respectively. The apparent ileal digestibilities of amino acids were similar between the rats and pigs although a significant (p