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Blood Storage

The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform

Chen Wang – 1st expert on this subject based on the ideXlab platform

  • Microfluidic measurement of RBC bending stiffness changes in Blood Storage
    2017 19th International Conference on Solid-State Sensors Actuators and Microsystems (TRANSDUCERS), 2017
    Co-Authors: Zhensong Xu, Huayan Pu, Chen Wang

    Abstract:

    In transfusion medicine, the deformability of stored red Blood cells (RBCs) changes during Storage in Blood banks. Compromised red Blood cell (RBC) deformability can lower the transfusion efficiency or intensify transfusion complications such as sepsis. The controversial results in present literature are caused by the differences in the mode of RBC deformation and the metrics for indicating RBC deformability. This paper reports microfluidic mechanical measurement of stored RBCs under the physiological deformation mode (i.e., bending). Instead of using phenomenological metrics of deformation or elongation indices (DI or EI), RBCs’ bending stiffness, a flow velocity independent parameter, is for the first time defined and used to evaluate the mechanical degradation of RBCs during Storage. Fresh RBCs and RBCs stored up to six weeks in the Blood bank were measured, revealing that the bending stiffness of RBCs increases over the Storage process. RBCs stored for one week already started to show significantly higher stiffness than fresh RBCs, and stored RBC stiffness degraded faster in the last three weeks than in the first three weeks.

  • characterization of red Blood cell deformability change during Blood Storage
    Lab on a Chip, 2014
    Co-Authors: Yi Zheng, Chen Wang, Jun Chen, Nadine Shehata

    Abstract:

    Stored red Blood cells (RBCs) show progressive deformability changes during Blood banking/Storage. Their deformability changes over an 8 weeks’ Storage period were measured using a microfluidic device. Hydrodynamic focusing controls the orientation and position of individual RBCs within the microchannel. High-speed imaging (5000 frames s−1) captures the dynamic deformation behavior of the cells, and together with automated image analysis, enables the characterization of over 1000 RBCs within 3 minutes. Multiple parameters including deformation index (DI), time constant (shape recovery rate), and RBC circularity were quantified. Compared to previous studies on stored RBC deformability, our results include a significantly higher number of cells (>1000 cells per sample vs. a few to tens of cells per sample) and, for the first time, reveal deformation changes of stored RBCs when traveling through human-capillary-like microchannels. Contrary to existing knowledge, our results demonstrate that the deformation index of RBCs under folding does not change significantly over Blood Storage. However, significant differences exist in time constants and circularity distribution widths, which can be used to quantify stored RBC quality or age.

  • Characterization of red Blood cell deformability change during Blood Storage
    2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS), 2014
    Co-Authors: Yi Zheng, Jun Chen, Nadine Shehata, Chen Wang

    Abstract:

    Stored red Blood cells (RBCs) show progressive deformability change during Blood banking/Storage. Their deformability change over an 8 weeks’ Storage period was measured in this work using a microfluidic device and high-speed imaging. Multiple parameters including deformation index (DI), time constant (shape recovery rate), and RBC circularity were quantified. Compared to previous RBC deformability studies reported in the literature, our results include a significantly higher number of cells (>1,000 cells/sample vs. a few to tens of cells/sample) and, for the first time, reveal deformation changes of stored RBCs when they travel through human-capillary-like microchannels. The correlation between deformability and morphology of stored RBCs is also presented.

Yi Zheng – 2nd expert on this subject based on the ideXlab platform

  • characterization of red Blood cell deformability change during Blood Storage
    Lab on a Chip, 2014
    Co-Authors: Yi Zheng, Chen Wang, Jun Chen, Nadine Shehata

    Abstract:

    Stored red Blood cells (RBCs) show progressive deformability changes during Blood banking/Storage. Their deformability changes over an 8 weeks’ Storage period were measured using a microfluidic device. Hydrodynamic focusing controls the orientation and position of individual RBCs within the microchannel. High-speed imaging (5000 frames s−1) captures the dynamic deformation behavior of the cells, and together with automated image analysis, enables the characterization of over 1000 RBCs within 3 minutes. Multiple parameters including deformation index (DI), time constant (shape recovery rate), and RBC circularity were quantified. Compared to previous studies on stored RBC deformability, our results include a significantly higher number of cells (>1000 cells per sample vs. a few to tens of cells per sample) and, for the first time, reveal deformation changes of stored RBCs when traveling through human-capillary-like microchannels. Contrary to existing knowledge, our results demonstrate that the deformation index of RBCs under folding does not change significantly over Blood Storage. However, significant differences exist in time constants and circularity distribution widths, which can be used to quantify stored RBC quality or age.

  • Characterization of red Blood cell deformability change during Blood Storage
    2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS), 2014
    Co-Authors: Yi Zheng, Jun Chen, Nadine Shehata, Chen Wang

    Abstract:

    Stored red Blood cells (RBCs) show progressive deformability change during Blood banking/Storage. Their deformability change over an 8 weeks’ Storage period was measured in this work using a microfluidic device and high-speed imaging. Multiple parameters including deformation index (DI), time constant (shape recovery rate), and RBC circularity were quantified. Compared to previous RBC deformability studies reported in the literature, our results include a significantly higher number of cells (>1,000 cells/sample vs. a few to tens of cells/sample) and, for the first time, reveal deformation changes of stored RBCs when they travel through human-capillary-like microchannels. The correlation between deformability and morphology of stored RBCs is also presented.

Nancy M Heddle – 3rd expert on this subject based on the ideXlab platform

  • effect of short term vs long term Blood Storage on mortality after transfusion
    The New England Journal of Medicine, 2016
    Co-Authors: Nancy M Heddle, Richard J Cook, Donald M Arnold, Rebecca Barty, Mark Crowther, P J Devereaux

    Abstract:

    BackgroundRandomized, controlled trials have suggested that the transfusion of Blood after prolonged Storage does not increase the risk of adverse outcomes among patients, although most of these trials were restricted to high-risk populations and were not powered to detect small but clinically important differences in mortality. We sought to find out whether the duration of Blood Storage would have an effect on mortality after transfusion in a general population of hospitalized patients. MethodsIn this pragmatic, randomized, controlled trial conducted at six hospitals in four countries, we randomly assigned patients who required a red-cell transfusion to receive Blood that had been stored for the shortest duration (short-term Storage group) or the longest duration (long-term Storage group) in a 1:2 ratio. Only patients with type A or O Blood were included in the primary analysis, since pilot data suggested that our goal of achieving a difference in the mean duration of Blood Storage of at least 10 days wo…