The Experts below are selected from a list of 30 Experts worldwide ranked by ideXlab platform

Barbara Richter - One of the best experts on this subject based on the ideXlab platform.

  • partition coefficient blood to plasma Ratio protein binding and short term stability of 11 nor δ9 carboxy tetrahydrocannabinol glucuronide
    Forensic Science International, 2002
    Co-Authors: Gisela Skopp, L Potsch, M Mauden, Barbara Richter
    Abstract:

    11-Nor-Delta(9)-carboxy tetrahydrocannabinol glucuronide (THCCOOglu) is a major metabolite of tetrahydrocannabinol in blood. Despite its mass spectrometric identification already in 1980, further physicochemical data of THCCOOglu have not been established. Therefore, the octanol/buffer partition coefficient P and the blood to plasma Ratio b/p for THCCOOglu concentRations of 100 and 500ng/ml were investigated. Protein binding of the glucuronide was established from spiked albumin solutions at a level of 250ng/ml as well as from authentic samples. The data were compared to those of 11-nor-Delta(9)-carboxy tetrahydrocannabinol (THCCOOH). In addition, the short-term stability of THCCOOglu in plasma at different storage temperatures was studied. Analysis was performed by LC/MS/MS. The glucuronide partition coefficient P (mean: 17.4 and 18.0 for 100 and 500ng/ml, respectively) was unexpectedly lipophilic at pH 7.4. Its blood to plasma Ratios averaged 0.62 and 0.68 at 100 and 500ng/ml, respectively. THCCOOglu was highly reversibly bound to albumin (mean: 97%), and the mean fraction bound did not differ from that determined from authentic samples. THCCOOglu degraded even at a storage temperature of 4 degrees C and THCCOOH was identified as a major decomposition product.

  • partition coefficient blood to plasma Ratio protein binding and short term stability of 11 nor δ9 carboxy tetrahydrocannabinol glucuronide
    Forensic Science International, 2002
    Co-Authors: Gisela Skopp, L Potsch, M Mauden, Barbara Richter
    Abstract:

    Abstract 11-Nor-Δ 9 -carboxy tetrahydrocannabinol glucuronide (THCCOOglu) is a major metabolite of tetrahydrocannabinol in blood. Despite its mass spectrometric identification already in 1980, further physicochemical data of THCCOOglu have not been established. Therefore, the octanol/buffer partition coefficient P and the blood to plasma Ratio b/p for THCCOOglu concentRations of 100 and 500 ng/ml were investigated. Protein binding of the glucuronide was established from spiked albumin solutions at a level of 250 ng/ml as well as from authentic samples. The data were compared to those of 11-nor-Δ 9 -carboxy tetrahydrocannabinol (THCCOOH). In addition, the short-term stability of THCCOOglu in plasma at different storage temperatures was studied. Analysis was performed by LC/MS/MS. The glucuronide partition coefficient P (mean: 17.4 and 18.0 for 100 and 500 ng/ml, respectively) was unexpectedly lipophilic at pH 7.4. Its blood to plasma Ratios averaged 0.62 and 0.68 at 100 and 500 ng/ml, respectively. THCCOOglu was highly reversibly bound to albumin (mean: 97%), and the mean fraction bound did not differ from that determined from authentic samples. THCCOOglu degraded even at a storage temperature of 4 °C and THCCOOH was identified as a major decomposition product.

Gisela Skopp - One of the best experts on this subject based on the ideXlab platform.

  • partition coefficient blood to plasma Ratio protein binding and short term stability of 11 nor δ9 carboxy tetrahydrocannabinol glucuronide
    Forensic Science International, 2002
    Co-Authors: Gisela Skopp, L Potsch, M Mauden, Barbara Richter
    Abstract:

    11-Nor-Delta(9)-carboxy tetrahydrocannabinol glucuronide (THCCOOglu) is a major metabolite of tetrahydrocannabinol in blood. Despite its mass spectrometric identification already in 1980, further physicochemical data of THCCOOglu have not been established. Therefore, the octanol/buffer partition coefficient P and the blood to plasma Ratio b/p for THCCOOglu concentRations of 100 and 500ng/ml were investigated. Protein binding of the glucuronide was established from spiked albumin solutions at a level of 250ng/ml as well as from authentic samples. The data were compared to those of 11-nor-Delta(9)-carboxy tetrahydrocannabinol (THCCOOH). In addition, the short-term stability of THCCOOglu in plasma at different storage temperatures was studied. Analysis was performed by LC/MS/MS. The glucuronide partition coefficient P (mean: 17.4 and 18.0 for 100 and 500ng/ml, respectively) was unexpectedly lipophilic at pH 7.4. Its blood to plasma Ratios averaged 0.62 and 0.68 at 100 and 500ng/ml, respectively. THCCOOglu was highly reversibly bound to albumin (mean: 97%), and the mean fraction bound did not differ from that determined from authentic samples. THCCOOglu degraded even at a storage temperature of 4 degrees C and THCCOOH was identified as a major decomposition product.

  • partition coefficient blood to plasma Ratio protein binding and short term stability of 11 nor δ9 carboxy tetrahydrocannabinol glucuronide
    Forensic Science International, 2002
    Co-Authors: Gisela Skopp, L Potsch, M Mauden, Barbara Richter
    Abstract:

    Abstract 11-Nor-Δ 9 -carboxy tetrahydrocannabinol glucuronide (THCCOOglu) is a major metabolite of tetrahydrocannabinol in blood. Despite its mass spectrometric identification already in 1980, further physicochemical data of THCCOOglu have not been established. Therefore, the octanol/buffer partition coefficient P and the blood to plasma Ratio b/p for THCCOOglu concentRations of 100 and 500 ng/ml were investigated. Protein binding of the glucuronide was established from spiked albumin solutions at a level of 250 ng/ml as well as from authentic samples. The data were compared to those of 11-nor-Δ 9 -carboxy tetrahydrocannabinol (THCCOOH). In addition, the short-term stability of THCCOOglu in plasma at different storage temperatures was studied. Analysis was performed by LC/MS/MS. The glucuronide partition coefficient P (mean: 17.4 and 18.0 for 100 and 500 ng/ml, respectively) was unexpectedly lipophilic at pH 7.4. Its blood to plasma Ratios averaged 0.62 and 0.68 at 100 and 500 ng/ml, respectively. THCCOOglu was highly reversibly bound to albumin (mean: 97%), and the mean fraction bound did not differ from that determined from authentic samples. THCCOOglu degraded even at a storage temperature of 4 °C and THCCOOH was identified as a major decomposition product.

M Mauden - One of the best experts on this subject based on the ideXlab platform.

  • partition coefficient blood to plasma Ratio protein binding and short term stability of 11 nor δ9 carboxy tetrahydrocannabinol glucuronide
    Forensic Science International, 2002
    Co-Authors: Gisela Skopp, L Potsch, M Mauden, Barbara Richter
    Abstract:

    11-Nor-Delta(9)-carboxy tetrahydrocannabinol glucuronide (THCCOOglu) is a major metabolite of tetrahydrocannabinol in blood. Despite its mass spectrometric identification already in 1980, further physicochemical data of THCCOOglu have not been established. Therefore, the octanol/buffer partition coefficient P and the blood to plasma Ratio b/p for THCCOOglu concentRations of 100 and 500ng/ml were investigated. Protein binding of the glucuronide was established from spiked albumin solutions at a level of 250ng/ml as well as from authentic samples. The data were compared to those of 11-nor-Delta(9)-carboxy tetrahydrocannabinol (THCCOOH). In addition, the short-term stability of THCCOOglu in plasma at different storage temperatures was studied. Analysis was performed by LC/MS/MS. The glucuronide partition coefficient P (mean: 17.4 and 18.0 for 100 and 500ng/ml, respectively) was unexpectedly lipophilic at pH 7.4. Its blood to plasma Ratios averaged 0.62 and 0.68 at 100 and 500ng/ml, respectively. THCCOOglu was highly reversibly bound to albumin (mean: 97%), and the mean fraction bound did not differ from that determined from authentic samples. THCCOOglu degraded even at a storage temperature of 4 degrees C and THCCOOH was identified as a major decomposition product.

  • partition coefficient blood to plasma Ratio protein binding and short term stability of 11 nor δ9 carboxy tetrahydrocannabinol glucuronide
    Forensic Science International, 2002
    Co-Authors: Gisela Skopp, L Potsch, M Mauden, Barbara Richter
    Abstract:

    Abstract 11-Nor-Δ 9 -carboxy tetrahydrocannabinol glucuronide (THCCOOglu) is a major metabolite of tetrahydrocannabinol in blood. Despite its mass spectrometric identification already in 1980, further physicochemical data of THCCOOglu have not been established. Therefore, the octanol/buffer partition coefficient P and the blood to plasma Ratio b/p for THCCOOglu concentRations of 100 and 500 ng/ml were investigated. Protein binding of the glucuronide was established from spiked albumin solutions at a level of 250 ng/ml as well as from authentic samples. The data were compared to those of 11-nor-Δ 9 -carboxy tetrahydrocannabinol (THCCOOH). In addition, the short-term stability of THCCOOglu in plasma at different storage temperatures was studied. Analysis was performed by LC/MS/MS. The glucuronide partition coefficient P (mean: 17.4 and 18.0 for 100 and 500 ng/ml, respectively) was unexpectedly lipophilic at pH 7.4. Its blood to plasma Ratios averaged 0.62 and 0.68 at 100 and 500 ng/ml, respectively. THCCOOglu was highly reversibly bound to albumin (mean: 97%), and the mean fraction bound did not differ from that determined from authentic samples. THCCOOglu degraded even at a storage temperature of 4 °C and THCCOOH was identified as a major decomposition product.

L Potsch - One of the best experts on this subject based on the ideXlab platform.

  • partition coefficient blood to plasma Ratio protein binding and short term stability of 11 nor δ9 carboxy tetrahydrocannabinol glucuronide
    Forensic Science International, 2002
    Co-Authors: Gisela Skopp, L Potsch, M Mauden, Barbara Richter
    Abstract:

    11-Nor-Delta(9)-carboxy tetrahydrocannabinol glucuronide (THCCOOglu) is a major metabolite of tetrahydrocannabinol in blood. Despite its mass spectrometric identification already in 1980, further physicochemical data of THCCOOglu have not been established. Therefore, the octanol/buffer partition coefficient P and the blood to plasma Ratio b/p for THCCOOglu concentRations of 100 and 500ng/ml were investigated. Protein binding of the glucuronide was established from spiked albumin solutions at a level of 250ng/ml as well as from authentic samples. The data were compared to those of 11-nor-Delta(9)-carboxy tetrahydrocannabinol (THCCOOH). In addition, the short-term stability of THCCOOglu in plasma at different storage temperatures was studied. Analysis was performed by LC/MS/MS. The glucuronide partition coefficient P (mean: 17.4 and 18.0 for 100 and 500ng/ml, respectively) was unexpectedly lipophilic at pH 7.4. Its blood to plasma Ratios averaged 0.62 and 0.68 at 100 and 500ng/ml, respectively. THCCOOglu was highly reversibly bound to albumin (mean: 97%), and the mean fraction bound did not differ from that determined from authentic samples. THCCOOglu degraded even at a storage temperature of 4 degrees C and THCCOOH was identified as a major decomposition product.

  • partition coefficient blood to plasma Ratio protein binding and short term stability of 11 nor δ9 carboxy tetrahydrocannabinol glucuronide
    Forensic Science International, 2002
    Co-Authors: Gisela Skopp, L Potsch, M Mauden, Barbara Richter
    Abstract:

    Abstract 11-Nor-Δ 9 -carboxy tetrahydrocannabinol glucuronide (THCCOOglu) is a major metabolite of tetrahydrocannabinol in blood. Despite its mass spectrometric identification already in 1980, further physicochemical data of THCCOOglu have not been established. Therefore, the octanol/buffer partition coefficient P and the blood to plasma Ratio b/p for THCCOOglu concentRations of 100 and 500 ng/ml were investigated. Protein binding of the glucuronide was established from spiked albumin solutions at a level of 250 ng/ml as well as from authentic samples. The data were compared to those of 11-nor-Δ 9 -carboxy tetrahydrocannabinol (THCCOOH). In addition, the short-term stability of THCCOOglu in plasma at different storage temperatures was studied. Analysis was performed by LC/MS/MS. The glucuronide partition coefficient P (mean: 17.4 and 18.0 for 100 and 500 ng/ml, respectively) was unexpectedly lipophilic at pH 7.4. Its blood to plasma Ratios averaged 0.62 and 0.68 at 100 and 500 ng/ml, respectively. THCCOOglu was highly reversibly bound to albumin (mean: 97%), and the mean fraction bound did not differ from that determined from authentic samples. THCCOOglu degraded even at a storage temperature of 4 °C and THCCOOH was identified as a major decomposition product.

Kevin P Bateman - One of the best experts on this subject based on the ideXlab platform.

  • merck s perspective on the implementation of dried blood spot technology in clinical drug development why when and how
    Bioanalysis, 2013
    Co-Authors: Eric Woolf, Nancy G B Agrawal, Prajakti A Kothare, Vincenzo Pucci, Kevin P Bateman
    Abstract:

    This paper communicates Merck’s thoughts on why, when and how to use dried blood spot (DBS) technology in a clinical setting, and provides a strategic approach, emphasizing the necessary steps, for successful clinical implementation of this microsampling technique. PK consideRation based on relevant in vitro data, that is, Blood-to-Plasma Ratio, hematocrit, plasma unbound fraction and/or blood cell partition, is suggested to be part of the decision tree on when to choose DBS as a surrogate matrix for PK analysis. A quick feasibility assessment addressing analytical challenges, including sensitivity, hematocrit impact and storage stability, needs to be evaluated before initiating DBS studies. Special attention should be paid to the clinical sample collection procedures to ensure data quality. Bridging studies are required to establish the correlation between plasma and DBS data to ensure that pooling of data from the various clinical studies can be used in population PK or PK/PD assessment. Seeking regulat...