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David Crews - One of the best experts on this subject based on the ideXlab platform.

  • Neural substrates for sexual and thermoregulatory behavior in the male leopard gecko, Eublepharis macularius.
    Brain Research, 2004
    Co-Authors: Nora Edwards, Lance J. Kriegsfeld, David Crews
    Abstract:

    The preoptic area-anterior hypothalamus (POAH) continuum is critical for the integration of environmental, physiological, and behavioral cues associated with reproduction in vertebrates. In the present study, radiofrequency lesions in the POAH abolished sexual behavior in the leopard gecko (Eublepharis macularius). Furthermore, results suggest a differential effect of POAH lesions on those behaviors regarded as appetitive (tail vibration and grip) and those regarded as consummatory (mounting and copulation), with consummatory behaviors being affected to a greater extent. E. macularius is an ectothermic vertebrate that modulates Body Temperature behaviorally relative to ambient Temperature. In vertebrates, the POAH is also an important integrator of thermoRegulation. Thus, the present study investigated whether lesions that disrupt reproductive behavior also disrupt Body Temperature Regulation. While virtually all males displayed diurnal rhythms in thermoregulatory behavior prior to surgery, this pattern was abolished in a small proportion of animals bearing POAH lesions. Lesions that abolished thermoregulatory rhythms involved the suprachiasmatic nucleus (SCN), whereas lesions confined to the POAH, while dramatically influencing sexual behavior, did not affect thermoregulatory rhythms or Temperature set point. Together, these findings identify the POAH as an important neural locus regulating sexual behavior but not thermoRegulation and suggest that the SCN acts as a pacemaker controlling daily behavioral Temperature Regulation in this species.

  • Research report Neural substrates for sexual and thermoregulatory behavior in the male leopard gecko, Eublepharis macularius
    2004
    Co-Authors: Nora Edwards, Lance J. Kriegsfeld, David Crews
    Abstract:

    The preoptic area–anterior hypothalamus (POAH) continuum is critical for the integration of environmental, physiological, and behavioral cues associated with reproduction in vertebrates. In the present study, radiofrequency lesions in the POAH abolished sexual behavior in the leopard gecko (Eublepharis macularius). Furthermore, results suggest a differential effect of POAH lesions on those behaviors regarded as appetitive (tail vibration and grip) and those regarded as consummatory (mounting and copulation), with consummatory behaviors being affected to a greater extent. E. macularius is an ectothermic vertebrate that modulates Body Temperature behaviorally relative to ambient Temperature. In vertebrates, the POAH is also an important integrator of thermoRegulation. Thus, the present study investigated whether lesions that disrupt reproductive behavior also disrupt Body Temperature Regulation. While virtually all males displayed diurnal rhythms in thermoregulatory behavior prior to surgery, this pattern was abolished in a small proportion of animals bearing POAH lesions. Lesions that abolished thermoregulatory rhythms involved the suprachiasmatic nucleus (SCN), whereas lesions confined to the POAH, while dramatically influencing sexual behavior, did not affect thermoregulatory rhythms or Temperature set point. Together, these findings identify the POAH as an important neural locus regulating sexual behavior but not thermoRegulation and suggest that the SCN acts as a pacemaker controlling daily behavioral Temperature Regulation in this species. D 2004 Elsevier B.V. All rights reserved. Theme: Neural basis of behavior Topic: Sexual and thermoregulatory behaviors

Nora Edwards - One of the best experts on this subject based on the ideXlab platform.

  • Neural substrates for sexual and thermoregulatory behavior in the male leopard gecko, Eublepharis macularius.
    Brain Research, 2004
    Co-Authors: Nora Edwards, Lance J. Kriegsfeld, David Crews
    Abstract:

    The preoptic area-anterior hypothalamus (POAH) continuum is critical for the integration of environmental, physiological, and behavioral cues associated with reproduction in vertebrates. In the present study, radiofrequency lesions in the POAH abolished sexual behavior in the leopard gecko (Eublepharis macularius). Furthermore, results suggest a differential effect of POAH lesions on those behaviors regarded as appetitive (tail vibration and grip) and those regarded as consummatory (mounting and copulation), with consummatory behaviors being affected to a greater extent. E. macularius is an ectothermic vertebrate that modulates Body Temperature behaviorally relative to ambient Temperature. In vertebrates, the POAH is also an important integrator of thermoRegulation. Thus, the present study investigated whether lesions that disrupt reproductive behavior also disrupt Body Temperature Regulation. While virtually all males displayed diurnal rhythms in thermoregulatory behavior prior to surgery, this pattern was abolished in a small proportion of animals bearing POAH lesions. Lesions that abolished thermoregulatory rhythms involved the suprachiasmatic nucleus (SCN), whereas lesions confined to the POAH, while dramatically influencing sexual behavior, did not affect thermoregulatory rhythms or Temperature set point. Together, these findings identify the POAH as an important neural locus regulating sexual behavior but not thermoRegulation and suggest that the SCN acts as a pacemaker controlling daily behavioral Temperature Regulation in this species.

  • Research report Neural substrates for sexual and thermoregulatory behavior in the male leopard gecko, Eublepharis macularius
    2004
    Co-Authors: Nora Edwards, Lance J. Kriegsfeld, David Crews
    Abstract:

    The preoptic area–anterior hypothalamus (POAH) continuum is critical for the integration of environmental, physiological, and behavioral cues associated with reproduction in vertebrates. In the present study, radiofrequency lesions in the POAH abolished sexual behavior in the leopard gecko (Eublepharis macularius). Furthermore, results suggest a differential effect of POAH lesions on those behaviors regarded as appetitive (tail vibration and grip) and those regarded as consummatory (mounting and copulation), with consummatory behaviors being affected to a greater extent. E. macularius is an ectothermic vertebrate that modulates Body Temperature behaviorally relative to ambient Temperature. In vertebrates, the POAH is also an important integrator of thermoRegulation. Thus, the present study investigated whether lesions that disrupt reproductive behavior also disrupt Body Temperature Regulation. While virtually all males displayed diurnal rhythms in thermoregulatory behavior prior to surgery, this pattern was abolished in a small proportion of animals bearing POAH lesions. Lesions that abolished thermoregulatory rhythms involved the suprachiasmatic nucleus (SCN), whereas lesions confined to the POAH, while dramatically influencing sexual behavior, did not affect thermoregulatory rhythms or Temperature set point. Together, these findings identify the POAH as an important neural locus regulating sexual behavior but not thermoRegulation and suggest that the SCN acts as a pacemaker controlling daily behavioral Temperature Regulation in this species. D 2004 Elsevier B.V. All rights reserved. Theme: Neural basis of behavior Topic: Sexual and thermoregulatory behaviors

Mitsuhiro Yoshioka - One of the best experts on this subject based on the ideXlab platform.

  • crispr cas9 mediated in vivo gene editing reveals that neuronal 5 ht1a receptors in the dorsal raphe nucleus contribute to Body Temperature Regulation in mice
    Brain Research, 2019
    Co-Authors: Naoya Nishitani, Yu Ohmura, Kazuki Nagayasu, Norihiro Shibui, Shuji Kaneko, Akiko Ohashi, Takayuki Yoshida, Akihiro Yamanaka, Mitsuhiro Yoshioka
    Abstract:

    Serotonin (5-HT) in the central nervous system regulates a variety of biological functions, from the basic homeostatic control to higher brain functions, by acting on fourteen known receptor subtypes. However, it is still usually unclear which receptor subtype is responsible for a specific function due to the lack of highly selective ligands for most of these receptors. Although 5-HT receptor knockout mice are useful, the brain-wide distribution of various receptors makes it difficult to dissect receptor functions in specific and brain regions and cell types. Recent advances in CRISPR/Cas9-mediated in vivo genome editing technology may overcome this problem. In this study, we constructed a viral vector expressing a single guide (sg)RNA targeting Htr1a (sgHtr1a) and Cre recombinase under the control of a neuron-specific promoter. Injection of the viral vector into the dorsal raphe nucleus (DRN) of Cre-dependent Cas9 knock-in mice induced Cre-dependent Cas9 expression mainly in DRN serotonin and GABA neurons. Mismatch cleavage assay and Sanger sequencing showed insertion or deletion formation at the target site. 5-HT1A receptor agonist-induced hypothermia was attenuated and antidepressant effect of a selective serotonin reuptake inhibitor (SSRI) was enhanced by microinjection of the viral vector expressing sgHtr1a into the DRN of Cre-dependent Cas9 knock-in mice. These results suggest that this in vivo CRISPR/Cas9-mediated 5-HT receptor gene knockout strategy provides a reliable and low-cost method for elucidating 5-HT receptor functions in specific cell types and brain regions. Further, we demonstrate that the neuronal 5-HT1A receptor in the DRN regulates Body Temperature and antidepressant effect of SSRI.

Lance J. Kriegsfeld - One of the best experts on this subject based on the ideXlab platform.

  • Neural substrates for sexual and thermoregulatory behavior in the male leopard gecko, Eublepharis macularius.
    Brain Research, 2004
    Co-Authors: Nora Edwards, Lance J. Kriegsfeld, David Crews
    Abstract:

    The preoptic area-anterior hypothalamus (POAH) continuum is critical for the integration of environmental, physiological, and behavioral cues associated with reproduction in vertebrates. In the present study, radiofrequency lesions in the POAH abolished sexual behavior in the leopard gecko (Eublepharis macularius). Furthermore, results suggest a differential effect of POAH lesions on those behaviors regarded as appetitive (tail vibration and grip) and those regarded as consummatory (mounting and copulation), with consummatory behaviors being affected to a greater extent. E. macularius is an ectothermic vertebrate that modulates Body Temperature behaviorally relative to ambient Temperature. In vertebrates, the POAH is also an important integrator of thermoRegulation. Thus, the present study investigated whether lesions that disrupt reproductive behavior also disrupt Body Temperature Regulation. While virtually all males displayed diurnal rhythms in thermoregulatory behavior prior to surgery, this pattern was abolished in a small proportion of animals bearing POAH lesions. Lesions that abolished thermoregulatory rhythms involved the suprachiasmatic nucleus (SCN), whereas lesions confined to the POAH, while dramatically influencing sexual behavior, did not affect thermoregulatory rhythms or Temperature set point. Together, these findings identify the POAH as an important neural locus regulating sexual behavior but not thermoRegulation and suggest that the SCN acts as a pacemaker controlling daily behavioral Temperature Regulation in this species.

  • Research report Neural substrates for sexual and thermoregulatory behavior in the male leopard gecko, Eublepharis macularius
    2004
    Co-Authors: Nora Edwards, Lance J. Kriegsfeld, David Crews
    Abstract:

    The preoptic area–anterior hypothalamus (POAH) continuum is critical for the integration of environmental, physiological, and behavioral cues associated with reproduction in vertebrates. In the present study, radiofrequency lesions in the POAH abolished sexual behavior in the leopard gecko (Eublepharis macularius). Furthermore, results suggest a differential effect of POAH lesions on those behaviors regarded as appetitive (tail vibration and grip) and those regarded as consummatory (mounting and copulation), with consummatory behaviors being affected to a greater extent. E. macularius is an ectothermic vertebrate that modulates Body Temperature behaviorally relative to ambient Temperature. In vertebrates, the POAH is also an important integrator of thermoRegulation. Thus, the present study investigated whether lesions that disrupt reproductive behavior also disrupt Body Temperature Regulation. While virtually all males displayed diurnal rhythms in thermoregulatory behavior prior to surgery, this pattern was abolished in a small proportion of animals bearing POAH lesions. Lesions that abolished thermoregulatory rhythms involved the suprachiasmatic nucleus (SCN), whereas lesions confined to the POAH, while dramatically influencing sexual behavior, did not affect thermoregulatory rhythms or Temperature set point. Together, these findings identify the POAH as an important neural locus regulating sexual behavior but not thermoRegulation and suggest that the SCN acts as a pacemaker controlling daily behavioral Temperature Regulation in this species. D 2004 Elsevier B.V. All rights reserved. Theme: Neural basis of behavior Topic: Sexual and thermoregulatory behaviors

Michael L. May - One of the best experts on this subject based on the ideXlab platform.

  • Body Temperature Regulation in the dragonfly, Arigomphus villosipes (Odonata: Anisoptera: Gomphidae)
    International Journal of Odonatology, 2017
    Co-Authors: Michael L. May
    Abstract:

    Regulation of thoracic muscle Temperature has been investigated in a number of dragonfly species but is poorly known in the large and diverse family, Gomphidae. Moreover, Temperatures of other Body...

  • Body Temperature Regulation in the dragonfly, Arigomphus villosipes (Odonata: Anisoptera: Gomphidae)
    2017
    Co-Authors: Michael L. May
    Abstract:

    Regulation of thoracic muscle Temperature has been investigated in a number of dragonfly species but is poorly known in the large and diverse family, Gomphidae. Moreover, Temperatures of other Body regions have been recorded in very few ectothermic insects. In addition, correlations among multiple components of thermoregulatory behavior have rarely been examined quantitatively. Here I examine thermoRegulation in Arigomphus villosipes, a medium-sized gomphid common at the shores of lakes and ponds in the northeastern USA. Measurements of the Temperatures of the thorax (Tth), head (Th) and abdomen (Tab), using standard "grab and jab" techniques, indicate that both Tth and Th are relatively independent of air Temperature (Ta). It is not clear whether Tth and Th are independently regulated, although some data suggest that they might be. Arigomphus villosipes can warm its thoracic musculature endothermically to maintain high Tth during cool Ta in conditions of low solar radiation intensity. However, Regulation is principally behavioral, involving variation in Body and wing postures and perhaps in perch substrate choice. Certain of these behaviors are closely associated to form suites of behavior that together are adapted to enhance or inhibit heating, while others are constrained by trade-offs with other functional demands. The former have a significant demonstrable effect on Tth. These combinations of behaviors results in a well-developed capacity for thermoRegulation, allowing the insects to expand their activity periods and choice of perches, and probably improving male performance during competitive chases of females prior to mating.