The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Zvi Schwartz - One of the best experts on this subject based on the ideXlab platform.
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osteoinductivity of demineralized Bone Matrix is independent of donor bisphosphonate use
Journal of Bone and Joint Surgery American Volume, 2011Co-Authors: Zvi Schwartz, Shawn A Hunter, Chad J. Ronholdt, Sharon L Hyzy, Moon Hae Sunwoo, Mark Moore, Barbara D BoyanAbstract:Background: Demineralized Bone Matrix is commonly used as a Bone graft substitute, either alone or to supplement an osteoconductive material, because of its osteoinductive properties. The aging of the population has led to an increase in the number of prospective donors of demineralized Bone Matrix who have taken bisphosphonates to prevent osteoclastmediated Bone resorption. The aim of this study was to determine whether oral bisphosphonate usage affects the osteoinductivity of demineralized Bone Matrix from donors. Methods: Sex-matched and age-matched pairs of samples were provided by four tissue banks (three or four pairs per bank). Demineralized Bone Matrix donors without bisphosphonate treatment had a mean age (and standard deviation) of 69.1 ± 2.5 years, and donors with bisphosphonate treatment had a mean age of 68.9 ± 2.0 years. Each pair included one donor known to have taken bisphosphonates and one who had not taken bisphosphonates. Demineralized Bone Matrix previously confirmed as osteoinductive was the positive control, and heat-inactivated demineralized Bone Matrix was the negative control. Demineralized Bone Matrix incubated with 1 mL of phosphate-buffered saline solution containing 0, 0.002, 2.0, or 2000 ng/mL of alendronate was also tested. Gelatin capsules containing 15 mg of demineralized Bone Matrix were implanted bilaterally in the gastrocnemius muscle of male nude mice (eight implants per group). The mice were killed thirty-five days after implantation, and hind limbs were recovered and processed for histological analysis. Osteoinductivity was measured with use of a qualitative score and by histomorphometry. Results: Nine of fifteen samples from donors who had had bisphosphonate treatment and ten of fifteen samples from patients who had not had bisphosphonate treatment were osteoinductive. Qualitative mean scores were comparable (1.7 ± 0.4 for those without bisphosphonates and 1.9 ± 0.7 for those with bisphosphonates). Osteoinductive demineralized Bone Matrix samples produced ossicles of comparable size, regardless of bisphosphonate usage. Histomorphometric measurements of the area of new Bone formation and residual demineralized Bone Matrix were also comparable. The addition of alendronate to control demineralized Bone Matrix did not affect its osteoinductivity. Conclusions: Demineralized Bone Matrix samples from donors treated with bisphosphonates and donors not treated with bisphosphonates have the same ability to induce Bone formation. However, it is not known if the quality of the new Bone is affected, with subsequent consequences affecting Bone remodeling. Clinical Relevance: These results show that demineralized Bone Matrix can be safe and effective as an osteoinductive material, even when the original Bone graft is obtained from donors who have used bisphosphonates.
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osteoinductivity of demineralized Bone Matrix is independent of donor bisphosphonate use
Journal of Bone and Joint Surgery American Volume, 2011Co-Authors: Zvi Schwartz, Shawn A Hunter, Chad J. Ronholdt, Sharon L Hyzy, Moon Hae Sunwoo, Mark Moore, Barbara D BoyanAbstract:Background: Demineralized Bone Matrix is commonly used as a Bone graft substitute, either alone or to supplement an osteoconductive material, because of its osteoinductive properties. The aging of the population has led to an increase in the number of prospective donors of demineralized Bone Matrix who have taken bisphosphonates to prevent osteoclastmediated Bone resorption. The aim of this study was to determine whether oral bisphosphonate usage affects the osteoinductivity of demineralized Bone Matrix from donors. Methods: Sex-matched and age-matched pairs of samples were provided by four tissue banks (three or four pairs per bank). Demineralized Bone Matrix donors without bisphosphonate treatment had a mean age (and standard deviation) of 69.1 ± 2.5 years, and donors with bisphosphonate treatment had a mean age of 68.9 ± 2.0 years. Each pair included one donor known to have taken bisphosphonates and one who had not taken bisphosphonates. Demineralized Bone Matrix previously confirmed as osteoinductive was the positive control, and heat-inactivated demineralized Bone Matrix was the negative control. Demineralized Bone Matrix incubated with 1 mL of phosphate-buffered saline solution containing 0, 0.002, 2.0, or 2000 ng/mL of alendronate was also tested. Gelatin capsules containing 15 mg of demineralized Bone Matrix were implanted bilaterally in the gastrocnemius muscle of male nude mice (eight implants per group). The mice were killed thirty-five days after implantation, and hind limbs were recovered and processed for histological analysis. Osteoinductivity was measured with use of a qualitative score and by histomorphometry. Results: Nine of fifteen samples from donors who had had bisphosphonate treatment and ten of fifteen samples from patients who had not had bisphosphonate treatment were osteoinductive. Qualitative mean scores were comparable (1.7 ± 0.4 for those without bisphosphonates and 1.9 ± 0.7 for those with bisphosphonates). Osteoinductive demineralized Bone Matrix samples produced ossicles of comparable size, regardless of bisphosphonate usage. Histomorphometric measurements of the area of new Bone formation and residual demineralized Bone Matrix were also comparable. The addition of alendronate to control demineralized Bone Matrix did not affect its osteoinductivity. Conclusions: Demineralized Bone Matrix samples from donors treated with bisphosphonates and donors not treated with bisphosphonates have the same ability to induce Bone formation. However, it is not known if the quality of the new Bone is affected, with subsequent consequences affecting Bone remodeling. Clinical Relevance: These results show that demineralized Bone Matrix can be safe and effective as an osteoinductive material, even when the original Bone graft is obtained from donors who have used bisphosphonates.
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platelet rich plasma inhibits demineralized Bone Matrix induced Bone formation in nude mice
Journal of Bone and Joint Surgery American Volume, 2007Co-Authors: Don M Ranly, Barbara D Boyan, Christoph H Lohmann, Domenico Andreacchio, Zvi SchwartzAbstract:Background: It is unclear whether platelet-rich plasma is a clinically effective adjunct to osteoinductive agents such as demineralized Bone Matrix. It contains platelet-derived growth factor (PDGF), which decreases osteoinduction by human demineralized Bone Matrix in nude-mouse muscle, suggesting that platelet-rich plasma may also have a negative impact. This study tested the hypothesis that platelet-rich plasma reduces demineralized Bone Matrix-induced Bone formation and that this effect varies with donor-dependent differences in platelet-rich plasma and demineralized Bone Matrix. Methods: Human platelet-rich plasma was prepared from blood from six men (average age [and standard error of the mean], 29.2 ± 2.4 years). Platelet numbers were determined, and growth factors were quantified before and after platelet activation. Human demineralized Bone Matrix from two donors (demineralized Bone Matrix-1 and demineralized Bone Matrix-2) was mixed with activated platelet-rich plasma and was implanted bilaterally in the gastrocnemius muscle in eighty male nude mice (eight implants per variable). Fifty-six days after implantation, the hindlimb calf muscles were harvested for histological analysis. Osteoinduction was evaluated with use of a qualitative score and morphometric measurements of ossicle size, new Bone formation, and residual demineralized Bone Matrix. Results: Compared with platelet-poor plasma, platelet-rich plasma preparations exhibited a fourfold increase in the platelet count, a fifteenfold increase in the amount of transforming growth factor-β, a sixfold increase in the amount of PDGF-BB, a fivefold increase in the amount of PDGF-AA, and a twofold increase in the amount of PDGF-AB. Demineralized Bone Matrix-1 was more osteoinductive than demineralized Bone Matrix-2, as determined on the basis of a greater ossicle area. The effect of platelet-rich plasma was either neutral or inhibitory depending on the demineralized Bone Matrix batch. When used with demineralized Bone Matrix-1, platelet-rich plasma did not alter the qualitative score or overall ossicle size, but it decreased the new Bone area. When used with demineralized Bone Matrix-2, platelet-rich plasma reduced the qualitative score, ossicle area, and new Bone area and increased the amount of residual demineralized Bone Matrix. The effects on osteoinduction also varied with the donor of the platelet-rich plasma. Conclusions: Platelet-rich plasma decreased the osteoinductivity of demineralized Bone Matrix implanted in immunocom-promised mice, and the activities of both demineralized Bone Matrix and platelet-rich plasma were donor-dependent. Clinical Relevance: Platelet-rich plasma may not be an appropriate adjunct to demineralized Bone Matrix in some clinical applications.
Anita Ferraretto - One of the best experts on this subject based on the ideXlab platform.
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Gastrointestinal digestates of Grana Padano and Trentingrana cheeses promote intestinal calcium uptake and extracellular Bone Matrix formation in vitro.
Food research international (Ottawa Ont.), 2016Co-Authors: Paola De Luca, Filippo Rossi, Ivano De Noni, Stefano Cattaneo, Sara Bruschi, Milda Stuknytė, Margherita Maggioni, Michela Bottani, Amelia Fiorilli, Anita FerrarettoAbstract:In the present work, Grana Padano (GP) and Trentingrana (TN) cheeses at different ripening time were in vitro digested. To study calcium uptake and utilization, the intact digestates (selected doses that do not alter cell viability and Transepithelial Electrical Resistance) were administered to Caco2/HT-29 70/30 cells, cultured on a semipermeable membrane in transwells, as a model of human intestinal epithelium. Intact digestates as well as the whole basolateral solutions (mimicking the passage of digestates through intestinal cells before reaching the blood flow and Bone) in parallel were further administered to human osteoblast-like cells SaOS-2 to study the extracellular Bone Matrix formation. In vitro digestates deriving from GP and TN promoted calcium uptake and extracellular Bone Matrix formation independently of both the cheese type and its ripening period (13, 19 or 26months). The present study reports the ability of whole digestates of GP and TN cheeses to improve intestinal calcium absorption and Bone Matrix formation in vitro. Once fully explored at Bone level, this finding could better support the role of cheese in ameliorating calcium deficiencies and associated diseases in vivo.
Doaa Adelkhattab - One of the best experts on this subject based on the ideXlab platform.
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development of a synthetic tissue engineered three dimensional printed bioceramic based Bone graft with homogenously distributed osteoblasts and mineralizing Bone Matrix in vitro
Journal of Tissue Engineering and Regenerative Medicine, 2018Co-Authors: Doaa Adelkhattab, Francesca Giacomini, Cynthia M Gomes, Ulf Linow, Barbara Peleska, Martin Hardt, Renate Gildenhaar, Georg Berger, Jens Günster, Michael StillerAbstract:Over the last decade there have been increasing efforts to develop 3D scaffolds for Bone tissue engineering from bioactive ceramics with 3D printing emerging as a promising technology. The overall objective of the present study was to generate a tissue engineered synthetic Bone graft with homogenously distributed osteoblasts and mineralizing Bone Matrix in vitro, thereby mimicking the advantageous properties of autogenous Bone grafts and facilitating usage for reconstructing segmental discontinuity defects in vivo. To this end, 3D scaffolds were developed from a silica containing calciumalkaliorthophosphate utilizing first a replica technique namely the Schwartzwalder Somers method (SSM), and second 3D printing, (i.e. rapid prototyping, RP). The mechanical and physical scaffold properties and their potential to facilitate homogenous colonization by osteogenic cells and extracellular Bone Matrix formation throughout the porous scaffold architecture were examined. To this end, osteoblastic cells were dynamically cultured for 7d on both scaffold types with two different concentrations of 1.5 and 3x106 cells/ml. The amount of cells and Bone Matrix formed and osteogenic marker expression were evaluated using hard tissue histology, immunohistochemical and histomorphometric analysis. 3D printed scaffolds (RPS) exhibited more micropores, greater compressive strength and silica release. RPS seeded with 3x106 cells/ml displayed greatest cell and extracellular Matrix formation, mineralization and osteocalcin expression. In conclusion, RPS displayed superior mechanical and biological properties and facilitated generating a tissue engineered synthetic Bone graft in vitro, which mimics the advantageous properties of autogenous Bone grafts, by containing homogenously distributed terminally differentiated osteoblasts and mineralizing Bone Matrix and therefore is suitable for subsequent in vivo implantation for regenerating segmental discontinuity Bone defects.
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development of a synthetic tissue engineered 3d printed calciumalkaliphosphate based Bone graft with homogenously distributed osteoblasts and mineralizing Bone Matrix in vitro
Key Engineering Materials, 2016Co-Authors: Doaa Adelkhattab, Francesca Giacomini, Cynthia M Gomes, Ulf Linow, Barbara Peleska, Martin Hardt, Renate Gildenhaar, Georg Berger, Jens Günster, Alireza HoushmandAbstract:Over the last decade there have been increasing efforts to develop adequate 3D scaffolds for Bone tissue engineering from bioactive ceramics with 3D printing emerging as a promising technology. The overall objective of the present study was to generate a tissue engineered synthetic Bone graft with homogenously distributed osteoblasts and mineralizing Bone Matrix in vitro, thereby mimicking the advantageous properties of autogenous Bone grafts and facilitating usage for reconstructing segmental discontinuity defects in vivo. To this end, 3D scaffolds were developed from a silica containing calciumalkaliorthophosphate (code: GB9S14) utilizing two different fabrication processes, first a replica technique (SSM), and second 3D printing (RP). The mechanical and physical properties of the scaffolds (porosity, compressive strength, solubility) and their potential to facilitate homogenous colonization by osteogenic cells and extracellular Bone Matrix formation throughout the porous scaffold architecture prior to in vivo implantation were examined. To this end, murine osteoblastic cells (MT3T3-E1) were dynamically seeded and cultured for 7 days on both scaffold types under perfusion with two different concentrations of 1.5 and 3x106 cells per ml. The amount of cells and extracellular Matrix formed and osteogenic marker expression were evaluated using hard tissue histology, immunohistochemical and histomorphometric analysis. SSM scaffolds (SSMS) displayed a significantly greater total porosity (86.9%) than RP scaffolds (RPS) (50%), while RPS exhibited significantly more open micropores, greater compressive strength and silica release. RPS seeded with a 3x106 cells per ml displayed greatest cell and extracellular Matrix formation, mineralization and osteocalcin expression. In conclusion, RPS displayed superior mechanical and biological properties and facilitated generating a tissue engineered synthetic Bone graft in vitro, which mimics the advantageous properties of autogenous Bone grafts, by containing homogenously distributed terminally differentiated osteoblasts and mineralizing Bone Matrix and therefore is suitable for subsequent in vivo implantation for regenerating segmental discontinuity Bone defects.
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development of a synthetic tissue engineered 3d printed calciumalkaliphosphate based Bone graft with homogenously distributed osteoblasts and mineralizing Bone Matrix in vitro
Key Engineering Materials, 2016Co-Authors: Doaa Adelkhattab, Francesca Giacomini, Cynthia M Gomes, Ulf Linow, Barbara Peleska, Martin Hardt, Renate Gildenhaar, Georg Berger, Jens Günster, Alireza HoushmandAbstract:Over the last decade there have been increasing efforts to develop adequate 3D scaffolds for Bone tissue engineering from bioactive ceramics with 3D printing emerging as a promising technology. The overall objective of the present study was to generate a tissue engineered synthetic Bone graft with homogenously distributed osteoblasts and mineralizing Bone Matrix in vitro, thereby mimicking the advantageous properties of autogenous Bone grafts and facilitating usage for reconstructing segmental discontinuity defects in vivo. To this end, 3D scaffolds were developed from a silica containing calciumalkaliorthophosphate (code: GB9S14) utilizing two different fabrication processes, first a replica technique (SSM), and second 3D printing (RP). The mechanical and physical properties of the scaffolds (porosity, compressive strength, solubility) and their potential to facilitate homogenous colonization by osteogenic cells and extracellular Bone Matrix formation throughout the porous scaffold architecture prior to in vivo implantation were examined. To this end, murine osteoblastic cells (MT3T3-E1) were dynamically seeded and cultured for 7 days on both scaffold types under perfusion with two different concentrations of 1.5 and 3x106 cells per ml. The amount of cells and extracellular Matrix formed and osteogenic marker expression were evaluated using hard tissue histology, immunohistochemical and histomorphometric analysis. SSM scaffolds (SSMS) displayed a significantly greater total porosity (86.9%) than RP scaffolds (RPS) (50%), while RPS exhibited significantly more open micropores, greater compressive strength and silica release. RPS seeded with a 3x106 cells per ml displayed greatest cell and extracellular Matrix formation, mineralization and osteocalcin expression. In conclusion, RPS displayed superior mechanical and biological properties and facilitated generating a tissue engineered synthetic Bone graft in vitro, which mimics the advantageous properties of autogenous Bone grafts, by containing homogenously distributed terminally differentiated osteoblasts and mineralizing Bone Matrix and therefore is suitable for subsequent in vivo implantation for regenerating segmental discontinuity Bone defects.
Barbara D Boyan - One of the best experts on this subject based on the ideXlab platform.
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osteoinductivity of demineralized Bone Matrix is independent of donor bisphosphonate use
Journal of Bone and Joint Surgery American Volume, 2011Co-Authors: Zvi Schwartz, Shawn A Hunter, Chad J. Ronholdt, Sharon L Hyzy, Moon Hae Sunwoo, Mark Moore, Barbara D BoyanAbstract:Background: Demineralized Bone Matrix is commonly used as a Bone graft substitute, either alone or to supplement an osteoconductive material, because of its osteoinductive properties. The aging of the population has led to an increase in the number of prospective donors of demineralized Bone Matrix who have taken bisphosphonates to prevent osteoclastmediated Bone resorption. The aim of this study was to determine whether oral bisphosphonate usage affects the osteoinductivity of demineralized Bone Matrix from donors. Methods: Sex-matched and age-matched pairs of samples were provided by four tissue banks (three or four pairs per bank). Demineralized Bone Matrix donors without bisphosphonate treatment had a mean age (and standard deviation) of 69.1 ± 2.5 years, and donors with bisphosphonate treatment had a mean age of 68.9 ± 2.0 years. Each pair included one donor known to have taken bisphosphonates and one who had not taken bisphosphonates. Demineralized Bone Matrix previously confirmed as osteoinductive was the positive control, and heat-inactivated demineralized Bone Matrix was the negative control. Demineralized Bone Matrix incubated with 1 mL of phosphate-buffered saline solution containing 0, 0.002, 2.0, or 2000 ng/mL of alendronate was also tested. Gelatin capsules containing 15 mg of demineralized Bone Matrix were implanted bilaterally in the gastrocnemius muscle of male nude mice (eight implants per group). The mice were killed thirty-five days after implantation, and hind limbs were recovered and processed for histological analysis. Osteoinductivity was measured with use of a qualitative score and by histomorphometry. Results: Nine of fifteen samples from donors who had had bisphosphonate treatment and ten of fifteen samples from patients who had not had bisphosphonate treatment were osteoinductive. Qualitative mean scores were comparable (1.7 ± 0.4 for those without bisphosphonates and 1.9 ± 0.7 for those with bisphosphonates). Osteoinductive demineralized Bone Matrix samples produced ossicles of comparable size, regardless of bisphosphonate usage. Histomorphometric measurements of the area of new Bone formation and residual demineralized Bone Matrix were also comparable. The addition of alendronate to control demineralized Bone Matrix did not affect its osteoinductivity. Conclusions: Demineralized Bone Matrix samples from donors treated with bisphosphonates and donors not treated with bisphosphonates have the same ability to induce Bone formation. However, it is not known if the quality of the new Bone is affected, with subsequent consequences affecting Bone remodeling. Clinical Relevance: These results show that demineralized Bone Matrix can be safe and effective as an osteoinductive material, even when the original Bone graft is obtained from donors who have used bisphosphonates.
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osteoinductivity of demineralized Bone Matrix is independent of donor bisphosphonate use
Journal of Bone and Joint Surgery American Volume, 2011Co-Authors: Zvi Schwartz, Shawn A Hunter, Chad J. Ronholdt, Sharon L Hyzy, Moon Hae Sunwoo, Mark Moore, Barbara D BoyanAbstract:Background: Demineralized Bone Matrix is commonly used as a Bone graft substitute, either alone or to supplement an osteoconductive material, because of its osteoinductive properties. The aging of the population has led to an increase in the number of prospective donors of demineralized Bone Matrix who have taken bisphosphonates to prevent osteoclastmediated Bone resorption. The aim of this study was to determine whether oral bisphosphonate usage affects the osteoinductivity of demineralized Bone Matrix from donors. Methods: Sex-matched and age-matched pairs of samples were provided by four tissue banks (three or four pairs per bank). Demineralized Bone Matrix donors without bisphosphonate treatment had a mean age (and standard deviation) of 69.1 ± 2.5 years, and donors with bisphosphonate treatment had a mean age of 68.9 ± 2.0 years. Each pair included one donor known to have taken bisphosphonates and one who had not taken bisphosphonates. Demineralized Bone Matrix previously confirmed as osteoinductive was the positive control, and heat-inactivated demineralized Bone Matrix was the negative control. Demineralized Bone Matrix incubated with 1 mL of phosphate-buffered saline solution containing 0, 0.002, 2.0, or 2000 ng/mL of alendronate was also tested. Gelatin capsules containing 15 mg of demineralized Bone Matrix were implanted bilaterally in the gastrocnemius muscle of male nude mice (eight implants per group). The mice were killed thirty-five days after implantation, and hind limbs were recovered and processed for histological analysis. Osteoinductivity was measured with use of a qualitative score and by histomorphometry. Results: Nine of fifteen samples from donors who had had bisphosphonate treatment and ten of fifteen samples from patients who had not had bisphosphonate treatment were osteoinductive. Qualitative mean scores were comparable (1.7 ± 0.4 for those without bisphosphonates and 1.9 ± 0.7 for those with bisphosphonates). Osteoinductive demineralized Bone Matrix samples produced ossicles of comparable size, regardless of bisphosphonate usage. Histomorphometric measurements of the area of new Bone formation and residual demineralized Bone Matrix were also comparable. The addition of alendronate to control demineralized Bone Matrix did not affect its osteoinductivity. Conclusions: Demineralized Bone Matrix samples from donors treated with bisphosphonates and donors not treated with bisphosphonates have the same ability to induce Bone formation. However, it is not known if the quality of the new Bone is affected, with subsequent consequences affecting Bone remodeling. Clinical Relevance: These results show that demineralized Bone Matrix can be safe and effective as an osteoinductive material, even when the original Bone graft is obtained from donors who have used bisphosphonates.
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platelet rich plasma inhibits demineralized Bone Matrix induced Bone formation in nude mice
Journal of Bone and Joint Surgery American Volume, 2007Co-Authors: Don M Ranly, Barbara D Boyan, Christoph H Lohmann, Domenico Andreacchio, Zvi SchwartzAbstract:Background: It is unclear whether platelet-rich plasma is a clinically effective adjunct to osteoinductive agents such as demineralized Bone Matrix. It contains platelet-derived growth factor (PDGF), which decreases osteoinduction by human demineralized Bone Matrix in nude-mouse muscle, suggesting that platelet-rich plasma may also have a negative impact. This study tested the hypothesis that platelet-rich plasma reduces demineralized Bone Matrix-induced Bone formation and that this effect varies with donor-dependent differences in platelet-rich plasma and demineralized Bone Matrix. Methods: Human platelet-rich plasma was prepared from blood from six men (average age [and standard error of the mean], 29.2 ± 2.4 years). Platelet numbers were determined, and growth factors were quantified before and after platelet activation. Human demineralized Bone Matrix from two donors (demineralized Bone Matrix-1 and demineralized Bone Matrix-2) was mixed with activated platelet-rich plasma and was implanted bilaterally in the gastrocnemius muscle in eighty male nude mice (eight implants per variable). Fifty-six days after implantation, the hindlimb calf muscles were harvested for histological analysis. Osteoinduction was evaluated with use of a qualitative score and morphometric measurements of ossicle size, new Bone formation, and residual demineralized Bone Matrix. Results: Compared with platelet-poor plasma, platelet-rich plasma preparations exhibited a fourfold increase in the platelet count, a fifteenfold increase in the amount of transforming growth factor-β, a sixfold increase in the amount of PDGF-BB, a fivefold increase in the amount of PDGF-AA, and a twofold increase in the amount of PDGF-AB. Demineralized Bone Matrix-1 was more osteoinductive than demineralized Bone Matrix-2, as determined on the basis of a greater ossicle area. The effect of platelet-rich plasma was either neutral or inhibitory depending on the demineralized Bone Matrix batch. When used with demineralized Bone Matrix-1, platelet-rich plasma did not alter the qualitative score or overall ossicle size, but it decreased the new Bone area. When used with demineralized Bone Matrix-2, platelet-rich plasma reduced the qualitative score, ossicle area, and new Bone area and increased the amount of residual demineralized Bone Matrix. The effects on osteoinduction also varied with the donor of the platelet-rich plasma. Conclusions: Platelet-rich plasma decreased the osteoinductivity of demineralized Bone Matrix implanted in immunocom-promised mice, and the activities of both demineralized Bone Matrix and platelet-rich plasma were donor-dependent. Clinical Relevance: Platelet-rich plasma may not be an appropriate adjunct to demineralized Bone Matrix in some clinical applications.
Melvin J Glimcher - One of the best experts on this subject based on the ideXlab platform.
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Bone Matrix imaged in vivo by water and fat suppressed proton projection mri waspi of animal and human subjects
Journal of Magnetic Resonance Imaging, 2010Co-Authors: Mirko I Hrovat, Haihui Cao, Jerome L Ackerman, Melvin J Glimcher, Timothy G Reese, Kirsten EcklundAbstract:Purpose: To demonstrate water- and fat-suppressed proton projection MRI (WASPI) in a clinical scanner to visualize the solid Bone Matrix in animal and human subjects. Materials and Methods: Pig Bone specimens and polymer pellets were used to optimize the WASPI method in terms of soft-tissue suppression, image resolution, signal-to-noise ratio, and scan time on a 3T MRI scanner. The ankles of healthy 2–3-month-old live Yorkshire pigs were scanned with the optimized method. The method was also applied to the wrists of six healthy adult human volunteers to demonstrate the feasibility of the WASPI method in human subjects. A transmit/receive coil built with proton-free materials was utilized to produce a strong B1 field. A fast transmit/receive switch was developed to reduce the long receiver dead time that would otherwise obscure the signals. Results: Clear 3D WASPI images of pig ankles and human wrists, showing only the solid Bone Matrix and other tissues with high solid content (eg, tendons), with a spatial resolution of 2.0 mm in all three dimensions were obtained in as briefly as 12 minutes. Conclusion: WASPI of the solid Matrix of Bone in humans and animals in vivo is feasible. J. Magn. Reson. Imaging 2010;31:954–963. ©2010 Wiley-Liss, Inc.
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quantitative Bone Matrix density measurement by water and fat suppressed proton projection mri waspi with polymer calibration phantoms
Magnetic Resonance in Medicine, 2008Co-Authors: Haihui Cao, Jerome L Ackerman, Mirko I Hrovat, Lila Graham, Melvin J GlimcherAbstract:The density of the organic Matrix of Bone substance is a critical parameter necessary to clinically evaluate and distinguish structural and metabolic pathological conditions such as osteomalacia in adults and rickets in growing children. Water and fat suppressed proton projection MRI (WASPI) was developed as a noninvasive means to obtain this information. In this study, a density calibration phantom was developed to convert WASPI intensity to true Bone Matrix density. The phantom contained a specifically designed poly(ethylene oxide)/poly(methyl methacrylate) blend, whose MRI properties (T1, T2 and resonance linewidth) were similar to those of solid Bone Matrix (collagen, tightly bound water, other immobile molecules), minimizing the need to correct for differences in T1 and/or T2 relaxation between the phantom and the subject. Cortical and trabecular porcine Bone specimens were imaged using WASPI with the calibration phantom in the field of view as a stable intensity reference. Gravimetric and amino acid analyses were carried out on the same specimens after WASPI and the chemical results were found to be highly correlated (r2 = 0.98 and 0.95 respectively) to the WASPI intensity. By this procedure the WASPI intensity can be used to obtain the true Bone Matrix mass density in g cm−3.