The Experts below are selected from a list of 2085 Experts worldwide ranked by ideXlab platform

Gail B. Mahady - One of the best experts on this subject based on the ideXlab platform.

  • Fractions of Boswellia serrata Suppress LTA4, LTC4, Cyclooxygenase-2 Activities and mRNA in HL-60 Cells and Reduce Lung Inflammation in BALB/c Mice.
    Current drug discovery technologies, 2021
    Co-Authors: Kapil K. Soni, Deepak Meshram, Temitope O. Lawal, Udeshi Patel, Gail B. Mahady
    Abstract:

    BACKGROUND Purified fractions from a Boswellia serrata Roxb. Ex. Colebr. (Burseraceae) extract (ETOH and DCM) contain biologically active compounds that are well known for having inflammation inhibitory properties. In this work, the purified fractions were tested in-vitro for LTC4, LTA4 and COX-2 activities using ELISA and qPCR was performed to determine gene regulation in human leukemia (HL-60) Cells. Two D-imaging tomography was performed to determine the anti-inflammatory activities of the fractions in BALB/c mouse model of lung inflammation. OBJECTIVE To evaluate anti-inflammatory activities of bioactive compounds of Boswellia serrata purified fractions. METHODS In-vitro MTT assay was performed in HL-60 cell lines for measuring the toxicity/ viability of the cells. ELISA tests were performed for evaluating LTA4, LTC4 and COX-2 activities. qPCR was performed to evaluate the expression of mRNA in HL-60 cells. In-vivo experiments were performed in OVA sensitized and challenged BALB/c mice at two doses of Boswellia serrata purified fraction containing 6% Boswellic acid of 50 and 100mg/kg body weight were given orally and the standard drug dexamethasone (DXA, 4 mg/kg body weight) and reduction in lung inflammation was assessed by using an IVIS Xenogen in-vivo fluorescence imaging system. RESULTS A purified fraction of Boswellia serrata ETOH extracts reduced leukotriene-C4-synthase activity by 52%, leuktotriene-A4-hydrolase activity by 22% and COX-2 activity by 99% with an IC50 of 12.5μg/ml. Intragastric administration of the purified fraction of Boswellia serrata at two doses of 50mg/kg b.w. and 100mg/kg b.w., respectively along with 2-3% HPMC resulted in a ~51% (P value

  • fractions of Boswellia serrata suppress lta4 ltc4 cyclooxygenase 2 activities and mrna in hl 60 cells and reduce lung inflammation in balb c mice
    Current Drug Discovery Technologies, 2021
    Co-Authors: Kapil K. Soni, Deepak Meshram, Temitope O. Lawal, Udeshi Patel, Gail B. Mahady
    Abstract:

    BACKGROUND Purified fractions from a Boswellia serrata Roxb. Ex. Colebr. (Burseraceae) extract (ETOH and DCM) contain biologically active compounds that are well known for having inflammation inhibitory properties. In this work, the purified fractions were tested in-vitro for LTC4, LTA4 and COX-2 activities using ELISA and qPCR was performed to determine gene regulation in human leukemia (HL-60) Cells. Two D-imaging tomography was performed to determine the anti-inflammatory activities of the fractions in BALB/c mouse model of lung inflammation. OBJECTIVE To evaluate anti-inflammatory activities of bioactive compounds of Boswellia serrata purified fractions. METHODS In-vitro MTT assay was performed in HL-60 cell lines for measuring the toxicity/ viability of the cells. ELISA tests were performed for evaluating LTA4, LTC4 and COX-2 activities. qPCR was performed to evaluate the expression of mRNA in HL-60 cells. In-vivo experiments were performed in OVA sensitized and challenged BALB/c mice at two doses of Boswellia serrata purified fraction containing 6% Boswellic acid of 50 and 100mg/kg body weight were given orally and the standard drug dexamethasone (DXA, 4 mg/kg body weight) and reduction in lung inflammation was assessed by using an IVIS Xenogen in-vivo fluorescence imaging system. RESULTS A purified fraction of Boswellia serrata ETOH extracts reduced leukotriene-C4-synthase activity by 52%, leuktotriene-A4-hydrolase activity by 22% and COX-2 activity by 99% with an IC50 of 12.5μg/ml. Intragastric administration of the purified fraction of Boswellia serrata at two doses of 50mg/kg b.w. and 100mg/kg b.w., respectively along with 2-3% HPMC resulted in a ~51% (P value <0.01) reduction in OVA induced lung inflammation in BALB/c mice as observed by imaging tomography. Treatment of the OVA challenged mice with standard drug dexamethasone (DXA) reduced inflammation by ~66% with significant value (P<0.0001). CONCLUSION The present study describes that Boswellia serrata ethanolic extracts purified fraction (ETOH-BS) possess significant anti-inflammatory activities in HL-60 and in BALB/c and further supports for its use as Ayurvedic medicines traditionally in the treatment of lung disorders including allergy and asthma.

Bikram Singh - One of the best experts on this subject based on the ideXlab platform.

  • Volatile constituents of natural Boswellia serrata oleo-gum-resin and commercial samples
    Flavour and Fragrance Journal, 2007
    Co-Authors: Bikram Singh, Siddharth Bhandari, Ripudaman Kumar, Shivani Pathania, Brij Lai
    Abstract:

    The essential oils of Boswellia serrata Roxb. ex Colebr. oleo-gum-resin collected from four different locations (samples A-D) were isolated by hydrodistillation and analysed by means of GC and GC-MS. Thirty-five components were identified, comprising 82%, 91%, 77% and 82% of samples A, B, C and D, respectively. α-Thujene was one of the major constituents in all samples, whereas α-pinene was recorded in only one sample. All the oils were dominated by monoterpene hydrocarbons. Copyright © 2006 John Wiley & Sons, Ltd.

  • Volatile constituents of natural Boswellia serrata oleo‐gum‐resin and commercial samples
    Flavour and Fragrance Journal, 2007
    Co-Authors: Bikram Singh, Siddharth Bhandari, Ripudaman Kumar, Shivani Pathania, Brij Lal
    Abstract:

    The essential oils of Boswellia serrata Roxb. ex Colebr. oleo-gum-resin collected from four different locations (samples A–D) were isolated by hydrodistillation and analysed by means of GC and GC–MS. Thirty-five components were identified, comprising 82%, 91%, 77% and 82% of samples A, B, C and D, respectively. α-Thujene was one of the major constituents in all samples, whereas α-pinene was recorded in only one sample. All the oils were dominated by monoterpene hydrocarbons. Copyright © 2006 John Wiley & Sons, Ltd.

Brij Lai - One of the best experts on this subject based on the ideXlab platform.

  • Volatile constituents of natural Boswellia serrata oleo-gum-resin and commercial samples
    Flavour and Fragrance Journal, 2007
    Co-Authors: Bikram Singh, Siddharth Bhandari, Ripudaman Kumar, Shivani Pathania, Brij Lai
    Abstract:

    The essential oils of Boswellia serrata Roxb. ex Colebr. oleo-gum-resin collected from four different locations (samples A-D) were isolated by hydrodistillation and analysed by means of GC and GC-MS. Thirty-five components were identified, comprising 82%, 91%, 77% and 82% of samples A, B, C and D, respectively. α-Thujene was one of the major constituents in all samples, whereas α-pinene was recorded in only one sample. All the oils were dominated by monoterpene hydrocarbons. Copyright © 2006 John Wiley & Sons, Ltd.

Brij Lal - One of the best experts on this subject based on the ideXlab platform.

  • Volatile constituents of natural Boswellia serrata oleo‐gum‐resin and commercial samples
    Flavour and Fragrance Journal, 2007
    Co-Authors: Bikram Singh, Siddharth Bhandari, Ripudaman Kumar, Shivani Pathania, Brij Lal
    Abstract:

    The essential oils of Boswellia serrata Roxb. ex Colebr. oleo-gum-resin collected from four different locations (samples A–D) were isolated by hydrodistillation and analysed by means of GC and GC–MS. Thirty-five components were identified, comprising 82%, 91%, 77% and 82% of samples A, B, C and D, respectively. α-Thujene was one of the major constituents in all samples, whereas α-pinene was recorded in only one sample. All the oils were dominated by monoterpene hydrocarbons. Copyright © 2006 John Wiley & Sons, Ltd.

Shivani Pathania - One of the best experts on this subject based on the ideXlab platform.

  • Volatile constituents of natural Boswellia serrata oleo-gum-resin and commercial samples
    Flavour and Fragrance Journal, 2007
    Co-Authors: Bikram Singh, Siddharth Bhandari, Ripudaman Kumar, Shivani Pathania, Brij Lai
    Abstract:

    The essential oils of Boswellia serrata Roxb. ex Colebr. oleo-gum-resin collected from four different locations (samples A-D) were isolated by hydrodistillation and analysed by means of GC and GC-MS. Thirty-five components were identified, comprising 82%, 91%, 77% and 82% of samples A, B, C and D, respectively. α-Thujene was one of the major constituents in all samples, whereas α-pinene was recorded in only one sample. All the oils were dominated by monoterpene hydrocarbons. Copyright © 2006 John Wiley & Sons, Ltd.

  • Volatile constituents of natural Boswellia serrata oleo‐gum‐resin and commercial samples
    Flavour and Fragrance Journal, 2007
    Co-Authors: Bikram Singh, Siddharth Bhandari, Ripudaman Kumar, Shivani Pathania, Brij Lal
    Abstract:

    The essential oils of Boswellia serrata Roxb. ex Colebr. oleo-gum-resin collected from four different locations (samples A–D) were isolated by hydrodistillation and analysed by means of GC and GC–MS. Thirty-five components were identified, comprising 82%, 91%, 77% and 82% of samples A, B, C and D, respectively. α-Thujene was one of the major constituents in all samples, whereas α-pinene was recorded in only one sample. All the oils were dominated by monoterpene hydrocarbons. Copyright © 2006 John Wiley & Sons, Ltd.