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Julia Maria Costacruz - One of the best experts on this subject based on the ideXlab platform.
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jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
Diagnostic Microbiology and Infectious Disease, 2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:Abstract The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC.
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parasitology jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC. © 2010 Elsevier Inc. All rights reserved.
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parasitology jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC. © 2010 Elsevier Inc. All rights reserved.
Daniela Da Silva Nunes - One of the best experts on this subject based on the ideXlab platform.
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jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
Diagnostic Microbiology and Infectious Disease, 2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:Abstract The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC.
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parasitology jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC. © 2010 Elsevier Inc. All rights reserved.
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parasitology jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC. © 2010 Elsevier Inc. All rights reserved.
Vanessa Da Silva Ribeiro - One of the best experts on this subject based on the ideXlab platform.
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jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
Diagnostic Microbiology and Infectious Disease, 2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:Abstract The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC.
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parasitology jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC. © 2010 Elsevier Inc. All rights reserved.
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parasitology jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC. © 2010 Elsevier Inc. All rights reserved.
Marianna Nascimento Manhani - One of the best experts on this subject based on the ideXlab platform.
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jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
Diagnostic Microbiology and Infectious Disease, 2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:Abstract The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC.
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parasitology jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC. © 2010 Elsevier Inc. All rights reserved.
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parasitology jacalin unBound Fraction of taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:The aim of this study was to evaluate jacalin-Bound Fraction (JBF) and jacalin-unBound Fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC. © 2010 Elsevier Inc. All rights reserved.
Bo Mattiasson - One of the best experts on this subject based on the ideXlab platform.
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protein like copolymers effect of polymer architecture on the performance in bioseparation process
Macromolecular Chemistry and Physics, 2002Co-Authors: Perolof Wahlund, Yu I Galaev, S A Kazakov, V I Lozinsky, Bo MattiassonAbstract:Full Paper: Recently, a new class of copolymers, socalled protein-like copolymers has been predicted theoretically by computer simulation. In these copolymers, the conformation of the copolymer determines the exposure of certain comonomer units to the outer solution. Depending on the conformation, copolymer molecules with essentially the same comonomer composition could have pronouncedly different properties. The authors demonstrated experimentally such behavior in case of poly[(N- vinylcaprolactam)-co-(N-vinylimidazole)] (Dokl. Chem. 2001, 375, 637). One more group of copolymers with protein-like behavior is copolymers of N-isopropylacrylamide with N-vinylimidazole. Poly[(N-isopropylacrylamide)-co-(N-vinylimidazole)] was synthesized by radical polymerization and separated into two Fractions using immobilized metal affinity chromatography on Cu 2 + - loaded iminodiacetic acid Sepharose CL 6B (Cu 2 + -IDA- sepharose). The unBound Fraction which passed through the column and Bound Fraction eluted with ethylenediaminetetraacetic acid, disodium salt (EDTA) solution differed significantly in molecular weight, 1.4 × 10 6 and 1.35 × 10 5 , respectively but were very close in comonomer composition, 7.8 and 9.1 mol-% of imidazole, respectively. The composition of Bound Fraction was confirmed by titration of imidazole groups. Despite close chemical composition, the Bound and unBound Fraction behaved differently with respect to temperature-induced phase separation at different pH values, the dependence of hydrodynamic diameter on pH and concentration of Cu 2 + - ions, and the coprecipitation of soybean trypsin inhibitor with the copolymer in the presence of Cu 2 + -ions. The differences in the behavior of copolymer Fractions are rationalized assuming that the Bound Fraction presents a protein-like copolymer.
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protein like copolymers effect of polymer architecture on the performance in bioseparation process
Macromolecular Bioscience, 2002Co-Authors: Perolof Wahlund, Yu I Galaev, S A Kazakov, V I Lozinsky, Bo MattiassonAbstract:Recently, a new class of copolymers, so-called protein-like copolymers has been predicted theoretically by computer simulation. In these copolymers. the conformation of the copolymer determines the exposure of certain comonomer units to the outer solution. Depending on the conformation, copolymer molecules with essentially the same comonomer composition could have pronouncedly different properties. The authors demonstrated experimentally such behavior in case of poly[(N- vinylcaprolactam)-co-(N-vinylimidazole)] (Dokl. Chem. 2001,375, 637). One more group of copolymers with protein-like behavior is copolymers of N-isopropylacryl-amide with N-vinylimidazole. Poly[(N-isopropylacryl-amide)-co-(N-vinylimidazole)] was synthesized by radical polymerization and separated into two Fractions using immobilized metal affinity chromatography on Cu2+-loaded iminodiacetic acid Sepbarose CL 6B (Cu2+-IDA-sepharose). The unBound Fraction which passed through the column and Bound Fraction eluted with ethylenediaminetetraacetic acid, disodium salt (EDTA) solution differed significantly in molecular weight, 1.4 x 10(6) and 1.35 x 10(5), respectively but were very close in comonomer composition, 7.8 and 9.1 mol-% of imidazole, respectively. The composition of Bound Fraction was confirmed by titration of imidazole groups. Despite close chemical composition, the Bound and unBound Fraction behaved differently with respect to temperature-induced phase separation at different pH values, the dependence of hydrodynamic diameter on pH and concentration of Cu2+- ions, and the coprecipitation of soybean trypsin inhibitor with the copolymer in the presence of Cu2+-ions. The differences in the behavior of copolymer Fractions are rationalized assuming that the Bound Fraction presents a protein-like copolymer. The dependence of hydrodynamic diameter of Bound (closed symbols) and unBound (open symbols) poly(NI-PAAM-VI) at different Cu2+/vinylimidazole ratios (n(Ca)/n(VI)), The polymer concentration was 4.5 mg (.) ml(-1) and pH 7.5 was obtained in all systems by using 0.010 m HEPES as a buffer. Mean values from five correlation functions are given. (Less)