The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Bahattin Çeliköz - One of the best experts on this subject based on the ideXlab platform.
-
use of lyophilized Bovine Collagen for split thickness skin graft donor site management
Burns, 2008Co-Authors: Fatih Uygur, Rahmi Evinc, Ersin Ülkür, Bahattin ÇeliközAbstract:Donor site management after split-thickness skin graft applications can have problems such as late healing and pain. Many dressing methods and medical applications are reported to solve these problems but none of them were ideal. In this study we aimed to promote epithelisation and remove pain earlier with using lyophilized Bovine Collagen (gelfix spray). According to our results, epithelisation time for the gelfix group was earlier than control group (9.09 days mean and 11.2 days mean for control group (p < 0.05)). Pain relief was determined by visual analogue pain scale. In the gelfix group, there was pain relief up to 40 h from the operation. There were no differences between groups for scarring 30 and 90 days after surgery.
-
Use of lyophilized Bovine Collagen for split-thickness skin graft donor site management.
Burns, 2008Co-Authors: Fatih Uygur, Rahmi Evinc, Ersin Ülkür, Bahattin ÇeliközAbstract:Donor site management after split-thickness skin graft applications can have problems such as late healing and pain. Many dressing methods and medical applications are reported to solve these problems but none of them were ideal. In this study we aimed to promote epithelisation and remove pain earlier with using lyophilized Bovine Collagen (gelfix spray). According to our results, epithelisation time for the gelfix group was earlier than control group (9.09 days mean and 11.2 days mean for control group (p
Margrethe Therkildsen - One of the best experts on this subject based on the ideXlab platform.
-
angiotensin i converting enzyme inhibitory peptides from Bovine Collagen insights into inhibitory mechanism and transepithelial transport
Food Research International, 2016Co-Authors: Yu Fu, Jette F. Young, Martin Krøyer Rasmussen, Trine Kastrup Dalsgaard, René Lametsch, Rotimi E. Aluko, Margrethe TherkildsenAbstract:Abstract The inhibitory mechanism and transepithelial transport of angiotensin I-converting enzyme (ACE)-inhibitory peptides (VGPV and GPRGF) derived from Alcalase®- and papain-hydrolyzed Bovine Collagen were investigated. The inhibitory mechanism of VGPV and GPRGF was experimentally determined to be non-competitive and the results were supported by molecular docking data. In silico and in vitro gastrointestinal digestion indicated that VGPV remained resistant to digestive enzymes, while GPRGF was degraded into smaller ACE-inhibitory peptides (GPR and GF). VGPV and GPRGF were transported across monolayers of human intestinal epithelial Caco-2 cells through paracellular pathway and retained their ACE-inhibitory activities. The present study suggests that VGPV and GPRGF may possibly be absorbed and exert antihypertensive effects in vivo.
-
Angiotensin I–converting enzyme–inhibitory peptides from Bovine Collagen: insights into inhibitory mechanism and transepithelial transport
Food Research International, 2016Co-Authors: Yu Fu, Jette F. Young, Martin Krøyer Rasmussen, Trine Kastrup Dalsgaard, René Lametsch, Rotimi E. Aluko, Margrethe TherkildsenAbstract:The inhibitory mechanism and transepithelial transport of angiotensin I-converting enzyme (ACE)-inhibitory peptides (VGPV and GPRGF) derived from Alcalase®- and papain-hydrolyzed Bovine Collagen were investigated. The inhibitory mechanism of VGPV and GPRGF was experimentally determined to be non-competitive and the results were supported by molecular docking data. In silico and in vitro gastrointestinal digestion indicated that VGPV remained resistant to digestive enzymes, while GPRGF was degraded into smaller ACE-inhibitory peptides (GPR and GF). VGPV and GPRGF were transported across monolayers of human intestinal epithelial Caco-2 cells through paracellular pathway and retained their ACE-inhibitory activities. The present study suggests that VGPV and GPRGF may possibly be absorbed and exert antihypertensive effects in vivo.
-
revalorisation of Bovine Collagen as a potential precursor of angiotensin i converting enzyme ace inhibitory peptides based on in silico and in vitro protein digestions
Journal of Functional Foods, 2016Co-Authors: Yu Fu, Jette F. Young, René Lametsch, Rotimi E. Aluko, Mette Marie Lokke, Margrethe TherkildsenAbstract:Abstract In silico proteolysis using 27 proteases theoretically released numerous ACE-inhibitory peptides from Collagen alpha-1(I) and alpha-2(I) sequences. Papain was the most effective protease to release ACE-inhibitory peptides. Two quantitative structure–activity relationship (QSAR) models for ACE-inhibitory peptides were established and employed to predict the activities of in silico-derived Collagen peptides. Furthermore, two promising in silico peptides (Tyr-Trp and Leu-Arg-Tyr) derived from papain and bromelain digestion were synthesised and experimentally confirmed as novel ACE inhibitors. In vitro digestion of Collagen by papain generated ACE-inhibitory peptides and the most active one was identified as a pentapeptide (Gly-Pro-Arg-Gly-Phe). However, Gly-Pro-Arg-Gly-Phe remained unidentified as the ACE-inhibitory peptide during the in silico digestion by papain mainly due to complete hydrolysis, which was not the case during in vitro digestion affected by external factors. Overall, the present study highlights Bovine Collagen as a promising precursor of ACE-inhibitory peptides by in silico and in vitro protein digestions.
Fatih Uygur - One of the best experts on this subject based on the ideXlab platform.
-
use of lyophilized Bovine Collagen for split thickness skin graft donor site management
Burns, 2008Co-Authors: Fatih Uygur, Rahmi Evinc, Ersin Ülkür, Bahattin ÇeliközAbstract:Donor site management after split-thickness skin graft applications can have problems such as late healing and pain. Many dressing methods and medical applications are reported to solve these problems but none of them were ideal. In this study we aimed to promote epithelisation and remove pain earlier with using lyophilized Bovine Collagen (gelfix spray). According to our results, epithelisation time for the gelfix group was earlier than control group (9.09 days mean and 11.2 days mean for control group (p < 0.05)). Pain relief was determined by visual analogue pain scale. In the gelfix group, there was pain relief up to 40 h from the operation. There were no differences between groups for scarring 30 and 90 days after surgery.
-
Use of lyophilized Bovine Collagen for split-thickness skin graft donor site management.
Burns, 2008Co-Authors: Fatih Uygur, Rahmi Evinc, Ersin Ülkür, Bahattin ÇeliközAbstract:Donor site management after split-thickness skin graft applications can have problems such as late healing and pain. Many dressing methods and medical applications are reported to solve these problems but none of them were ideal. In this study we aimed to promote epithelisation and remove pain earlier with using lyophilized Bovine Collagen (gelfix spray). According to our results, epithelisation time for the gelfix group was earlier than control group (9.09 days mean and 11.2 days mean for control group (p
Christine Radtke - One of the best experts on this subject based on the ideXlab platform.
-
cultivation of keratinocytes and fibroblasts in a three dimensional Bovine Collagen elastin matrix matriderm and application for full thickness wound coverage in vivo
International Journal of Molecular Sciences, 2013Co-Authors: Jasper Killat, Kerstin Reimers, Claudia Y U Choi, Sabrina Jahn, Peter M Vogt, Christine RadtkeAbstract:New skin substitutes for burn medicine or reconstructive surgery pose an important issue in plastic surgery. Matriderm® is a clinically approved three-dimensional Bovine Collagen-elastin matrix which is already used as a dermal substitute of full thickness burn wounds. The drawback of an avital matrix is the limited integration in full thickness skin defects, depending on the defect size. To further optimize this process, Matriderm® has also been studied as a matrix for tissue engineering of skin albeit long-term cultivation of the matrix with cells has been difficult. Cells have generally been seeded onto the matrix with high cell loss and minimal time-consuming migration. Here we developed a cell seeded skin equivalent after microtransfer of cells directly into the matrix. First, cells were cultured, and microinjected into Matriderm®. Then, cell viability in the matrix was determined by histology in vitro. As a next step, the skin substitute was applied in vivo into a full thickness rodent wound model. The wound coverage and healing was observed over a period of two weeks followed by histological examination assessing cell viability, proliferation and integration into the host. Viable and proliferating cells could be found throughout the entire matrix. The presented skin substitute resembles healthy skin in morphology and integrity. Based on this study, future investigations are planned to examine behaviour of epidermal stem cells injected into a Collagen-elastin matrix under the aspects of establishment of stem cell niches and differentiation.
Yu Fu - One of the best experts on this subject based on the ideXlab platform.
-
angiotensin i converting enzyme inhibitory peptides from Bovine Collagen insights into inhibitory mechanism and transepithelial transport
Food Research International, 2016Co-Authors: Yu Fu, Jette F. Young, Martin Krøyer Rasmussen, Trine Kastrup Dalsgaard, René Lametsch, Rotimi E. Aluko, Margrethe TherkildsenAbstract:Abstract The inhibitory mechanism and transepithelial transport of angiotensin I-converting enzyme (ACE)-inhibitory peptides (VGPV and GPRGF) derived from Alcalase®- and papain-hydrolyzed Bovine Collagen were investigated. The inhibitory mechanism of VGPV and GPRGF was experimentally determined to be non-competitive and the results were supported by molecular docking data. In silico and in vitro gastrointestinal digestion indicated that VGPV remained resistant to digestive enzymes, while GPRGF was degraded into smaller ACE-inhibitory peptides (GPR and GF). VGPV and GPRGF were transported across monolayers of human intestinal epithelial Caco-2 cells through paracellular pathway and retained their ACE-inhibitory activities. The present study suggests that VGPV and GPRGF may possibly be absorbed and exert antihypertensive effects in vivo.
-
Angiotensin I–converting enzyme–inhibitory peptides from Bovine Collagen: insights into inhibitory mechanism and transepithelial transport
Food Research International, 2016Co-Authors: Yu Fu, Jette F. Young, Martin Krøyer Rasmussen, Trine Kastrup Dalsgaard, René Lametsch, Rotimi E. Aluko, Margrethe TherkildsenAbstract:The inhibitory mechanism and transepithelial transport of angiotensin I-converting enzyme (ACE)-inhibitory peptides (VGPV and GPRGF) derived from Alcalase®- and papain-hydrolyzed Bovine Collagen were investigated. The inhibitory mechanism of VGPV and GPRGF was experimentally determined to be non-competitive and the results were supported by molecular docking data. In silico and in vitro gastrointestinal digestion indicated that VGPV remained resistant to digestive enzymes, while GPRGF was degraded into smaller ACE-inhibitory peptides (GPR and GF). VGPV and GPRGF were transported across monolayers of human intestinal epithelial Caco-2 cells through paracellular pathway and retained their ACE-inhibitory activities. The present study suggests that VGPV and GPRGF may possibly be absorbed and exert antihypertensive effects in vivo.
-
revalorisation of Bovine Collagen as a potential precursor of angiotensin i converting enzyme ace inhibitory peptides based on in silico and in vitro protein digestions
Journal of Functional Foods, 2016Co-Authors: Yu Fu, Jette F. Young, René Lametsch, Rotimi E. Aluko, Mette Marie Lokke, Margrethe TherkildsenAbstract:Abstract In silico proteolysis using 27 proteases theoretically released numerous ACE-inhibitory peptides from Collagen alpha-1(I) and alpha-2(I) sequences. Papain was the most effective protease to release ACE-inhibitory peptides. Two quantitative structure–activity relationship (QSAR) models for ACE-inhibitory peptides were established and employed to predict the activities of in silico-derived Collagen peptides. Furthermore, two promising in silico peptides (Tyr-Trp and Leu-Arg-Tyr) derived from papain and bromelain digestion were synthesised and experimentally confirmed as novel ACE inhibitors. In vitro digestion of Collagen by papain generated ACE-inhibitory peptides and the most active one was identified as a pentapeptide (Gly-Pro-Arg-Gly-Phe). However, Gly-Pro-Arg-Gly-Phe remained unidentified as the ACE-inhibitory peptide during the in silico digestion by papain mainly due to complete hydrolysis, which was not the case during in vitro digestion affected by external factors. Overall, the present study highlights Bovine Collagen as a promising precursor of ACE-inhibitory peptides by in silico and in vitro protein digestions.