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F R Spilki - One of the best experts on this subject based on the ideXlab platform.
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Detection of Bovine Herpesvirus 2 and Bovine Herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction.
Veterinary microbiology, 2014Co-Authors: F S Campos, A C Franco, M T Oliveira, R Firpo, G Strelczuk, F E Fontoura, M I R Kulmann, S Maidana, S A Romera, F R SpilkiAbstract:Establishment of latent infection within specific tissues in the host is a common biological feature of the Herpesviruses. In the case of Bovine Herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while Bovine Herpesvirus 4 (BoHV-4) and ovine Herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with Bovine Herpesvirus 1 (BoHV-1) and Bovine Herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct Bovine Herpesviruses might be a naturally occurring phenomenon.
F S Campos - One of the best experts on this subject based on the ideXlab platform.
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Detection of Bovine Herpesvirus 2 and Bovine Herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction.
Veterinary microbiology, 2014Co-Authors: F S Campos, A C Franco, M T Oliveira, R Firpo, G Strelczuk, F E Fontoura, M I R Kulmann, S Maidana, S A Romera, F R SpilkiAbstract:Establishment of latent infection within specific tissues in the host is a common biological feature of the Herpesviruses. In the case of Bovine Herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while Bovine Herpesvirus 4 (BoHV-4) and ovine Herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with Bovine Herpesvirus 1 (BoHV-1) and Bovine Herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct Bovine Herpesviruses might be a naturally occurring phenomenon.
A C Franco - One of the best experts on this subject based on the ideXlab platform.
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Detection of Bovine Herpesvirus 2 and Bovine Herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction.
Veterinary microbiology, 2014Co-Authors: F S Campos, A C Franco, M T Oliveira, R Firpo, G Strelczuk, F E Fontoura, M I R Kulmann, S Maidana, S A Romera, F R SpilkiAbstract:Establishment of latent infection within specific tissues in the host is a common biological feature of the Herpesviruses. In the case of Bovine Herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while Bovine Herpesvirus 4 (BoHV-4) and ovine Herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with Bovine Herpesvirus 1 (BoHV-1) and Bovine Herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct Bovine Herpesviruses might be a naturally occurring phenomenon.
M T Oliveira - One of the best experts on this subject based on the ideXlab platform.
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Detection of Bovine Herpesvirus 2 and Bovine Herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction.
Veterinary microbiology, 2014Co-Authors: F S Campos, A C Franco, M T Oliveira, R Firpo, G Strelczuk, F E Fontoura, M I R Kulmann, S Maidana, S A Romera, F R SpilkiAbstract:Establishment of latent infection within specific tissues in the host is a common biological feature of the Herpesviruses. In the case of Bovine Herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while Bovine Herpesvirus 4 (BoHV-4) and ovine Herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with Bovine Herpesvirus 1 (BoHV-1) and Bovine Herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct Bovine Herpesviruses might be a naturally occurring phenomenon.
R Firpo - One of the best experts on this subject based on the ideXlab platform.
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Detection of Bovine Herpesvirus 2 and Bovine Herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction.
Veterinary microbiology, 2014Co-Authors: F S Campos, A C Franco, M T Oliveira, R Firpo, G Strelczuk, F E Fontoura, M I R Kulmann, S Maidana, S A Romera, F R SpilkiAbstract:Establishment of latent infection within specific tissues in the host is a common biological feature of the Herpesviruses. In the case of Bovine Herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while Bovine Herpesvirus 4 (BoHV-4) and ovine Herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with Bovine Herpesvirus 1 (BoHV-1) and Bovine Herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct Bovine Herpesviruses might be a naturally occurring phenomenon.