The Experts below are selected from a list of 37035 Experts worldwide ranked by ideXlab platform

S. Umapathi - One of the best experts on this subject based on the ideXlab platform.

  • PVA and BSA stabilized silver nanoparticles based surface-enhanced plasmon resonance probes for protein detection.
    Colloids and surfaces. B Biointerfaces, 2011
    Co-Authors: A. Nimrodh Ananth, S.c.g. Kiruba Daniel, T. Anitha Sironmani, S. Umapathi
    Abstract:

    To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420 nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443 nm) when they were coated with BSA. The biointeractions of these engineered nanoparticles were studied using a mouse model. No significant changes in behavior or toxicity were observed. The nanoparticles were detected in all tissues including the Brain. Antibody recognition was monitored via the change in light absorption which accompanied binding, indicating that the particles can be used as a biosensor to gain more insight into cellular mechanisms governing the function of organs in general, and the blood Brain barrier (BBB) and Brain in particular.

  • PVA and BSA stabilized silver nanoparticles based surface–enhanced plasmon resonance probes for protein detection B Biointerfaces
    Colloids and Surfaces, 2011
    Co-Authors: A. Nimrodh Ananth, S.c.g. Kiruba Daniel, T. Anitha Sironmani, S. Umapathi
    Abstract:

    To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443nm) when they were coated with BSA. The biointeractions of these engineered nanoparticles were studied using a mouse model. No significant changes in behavior or toxicity were observed. The nanoparticles were detected in all tissues including the Brain. Antibody recognition was monitored via the change in light absorption which accompanied binding, indicating that the particles can be used as a biosensor to gain more insight into cellular mechanisms governing the function of organs in general, and the blood Brain barrier (BBB) and Brain in particular.

Lluis F. Marsal - One of the best experts on this subject based on the ideXlab platform.

  • A photoelectrochemical sandwich immunoassay for protein S100β, a biomarker for Alzheimer’s disease, using an ITO electrode modified with a reduced graphene oxide-gold conjugate and CdS-labeled secondary Antibody
    Mikrochimica Acta, 2019
    Co-Authors: Mahmoud Amouzadeh Tabrizi, Josep Ferré-borrull, Pankaj Kapruwan, Lluis F. Marsal
    Abstract:

    A sandwich-type photoelectrochemical immunoassay is described for the protein S100s which is an Alzheimer’s disease biomarker found in the astrocytes of the Brain. Antibody against S100s (anti-S100s) was labeled with CdS quantum dots and then acted as a secondary Antibody. The labeled Antibody was characterized by FTIR, ultraviolet-visible and fluorescence spectroscopy. An indium-tin oxide (ITO) electrode was modified with a nanocomposite prepared from reduced graphene oxide and gold nanoparticles. Then, a sol-gel film containing isocyanate functional groups (-N=C=O) was cast on the surface of the electrode. The NCO group reacts with amino groups of the labeled Antibody to covalently bind them to the surface. The S100β was bound by the primary immobilized Antibody on the rGO-Au/ITO electrode and then sandwiched with the labeled secondary Antibody. Cyclic voltammetry and electrochemical impedance spectroscopy were applied to confirm the stepwise changes in the electrochemical properties of the electrode surface. The photoelectrochemical immunoassay, typically operated at a potential of +0.2 V (vs. Ag|AgClsat) gives a signal that is related to the logarithm of the S100β concentration in the range from 0.25 to 10 ng·mL−1 with a lower detection limit of 0.15 pg·mL−1. The method was successfully applied to the determination of S100β in human serum samples.

  • A photoelectrochemical sandwich immunoassay for protein S100β, a biomarker for Alzheimer’s disease, using an ITO electrode modified with a reduced graphene oxide-gold conjugate and CdS-labeled secondary Antibody
    Microchimica Acta, 2019
    Co-Authors: Mahmoud Amouzadeh Tabrizi, Josep Ferré-borrull, Pankaj Kapruwan, Lluis F. Marsal
    Abstract:

    A sandwich-type photoelectrochemical immunoassay is described for the protein S100ß which is an Alzheimer’s disease biomarker found in the astrocytes of the Brain. Antibody against S100ß (anti-S100ß) was labeled with CdS quantum dots and then acted as a secondary Antibody. The labeled Antibody was characterized by FTIR, ultraviolet-visible and fluorescence spectroscopy. An indium-tin oxide (ITO) electrode was modified with a nanocomposite prepared from reduced graphene oxide and gold nanoparticles. Then, a sol-gel film containing isocyanate functional groups (-N=C=O) was cast on the surface of the electrode. The NCO group reacts with amino groups of the labeled Antibody to covalently bind them to the surface. The S100β was bound by the primary immobilized Antibody on the rGO-Au/ITO electrode and then sandwiched with the labeled secondary Antibody. Cyclic voltammetry and electrochemical impedance spectroscopy were applied to confirm the stepwise changes in the electrochemical properties of the electrode surface. The photoelectrochemical immunoassay, typically operated at a potential of +0.2 V (vs. Ag|AgCl_sat) gives a signal that is related to the logarithm of the S100β concentration in the range from 0.25 to 10 ng·mL^−1 with a lower detection limit of 0.15 pg·mL^−1. The method was successfully applied to the determination of S100β in human serum samples. Graphical abstract Schematic presentation of an immunosensor which is based on an indium tin oxide modified with reduced graphene oxide decorated with gold nanocomposite and Antibody. The immunosensor was applied for the determination of S100β biomarker by using in the labeled Antibody.

A. Nimrodh Ananth - One of the best experts on this subject based on the ideXlab platform.

  • PVA and BSA stabilized silver nanoparticles based surface-enhanced plasmon resonance probes for protein detection.
    Colloids and surfaces. B Biointerfaces, 2011
    Co-Authors: A. Nimrodh Ananth, S.c.g. Kiruba Daniel, T. Anitha Sironmani, S. Umapathi
    Abstract:

    To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420 nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443 nm) when they were coated with BSA. The biointeractions of these engineered nanoparticles were studied using a mouse model. No significant changes in behavior or toxicity were observed. The nanoparticles were detected in all tissues including the Brain. Antibody recognition was monitored via the change in light absorption which accompanied binding, indicating that the particles can be used as a biosensor to gain more insight into cellular mechanisms governing the function of organs in general, and the blood Brain barrier (BBB) and Brain in particular.

  • PVA and BSA stabilized silver nanoparticles based surface–enhanced plasmon resonance probes for protein detection B Biointerfaces
    Colloids and Surfaces, 2011
    Co-Authors: A. Nimrodh Ananth, S.c.g. Kiruba Daniel, T. Anitha Sironmani, S. Umapathi
    Abstract:

    To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443nm) when they were coated with BSA. The biointeractions of these engineered nanoparticles were studied using a mouse model. No significant changes in behavior or toxicity were observed. The nanoparticles were detected in all tissues including the Brain. Antibody recognition was monitored via the change in light absorption which accompanied binding, indicating that the particles can be used as a biosensor to gain more insight into cellular mechanisms governing the function of organs in general, and the blood Brain barrier (BBB) and Brain in particular.

T. Anitha Sironmani - One of the best experts on this subject based on the ideXlab platform.

  • PVA and BSA stabilized silver nanoparticles based surface-enhanced plasmon resonance probes for protein detection.
    Colloids and surfaces. B Biointerfaces, 2011
    Co-Authors: A. Nimrodh Ananth, S.c.g. Kiruba Daniel, T. Anitha Sironmani, S. Umapathi
    Abstract:

    To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420 nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443 nm) when they were coated with BSA. The biointeractions of these engineered nanoparticles were studied using a mouse model. No significant changes in behavior or toxicity were observed. The nanoparticles were detected in all tissues including the Brain. Antibody recognition was monitored via the change in light absorption which accompanied binding, indicating that the particles can be used as a biosensor to gain more insight into cellular mechanisms governing the function of organs in general, and the blood Brain barrier (BBB) and Brain in particular.

  • PVA and BSA stabilized silver nanoparticles based surface–enhanced plasmon resonance probes for protein detection B Biointerfaces
    Colloids and Surfaces, 2011
    Co-Authors: A. Nimrodh Ananth, S.c.g. Kiruba Daniel, T. Anitha Sironmani, S. Umapathi
    Abstract:

    To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443nm) when they were coated with BSA. The biointeractions of these engineered nanoparticles were studied using a mouse model. No significant changes in behavior or toxicity were observed. The nanoparticles were detected in all tissues including the Brain. Antibody recognition was monitored via the change in light absorption which accompanied binding, indicating that the particles can be used as a biosensor to gain more insight into cellular mechanisms governing the function of organs in general, and the blood Brain barrier (BBB) and Brain in particular.

S.c.g. Kiruba Daniel - One of the best experts on this subject based on the ideXlab platform.

  • PVA and BSA stabilized silver nanoparticles based surface-enhanced plasmon resonance probes for protein detection.
    Colloids and surfaces. B Biointerfaces, 2011
    Co-Authors: A. Nimrodh Ananth, S.c.g. Kiruba Daniel, T. Anitha Sironmani, S. Umapathi
    Abstract:

    To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420 nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443 nm) when they were coated with BSA. The biointeractions of these engineered nanoparticles were studied using a mouse model. No significant changes in behavior or toxicity were observed. The nanoparticles were detected in all tissues including the Brain. Antibody recognition was monitored via the change in light absorption which accompanied binding, indicating that the particles can be used as a biosensor to gain more insight into cellular mechanisms governing the function of organs in general, and the blood Brain barrier (BBB) and Brain in particular.

  • PVA and BSA stabilized silver nanoparticles based surface–enhanced plasmon resonance probes for protein detection B Biointerfaces
    Colloids and Surfaces, 2011
    Co-Authors: A. Nimrodh Ananth, S.c.g. Kiruba Daniel, T. Anitha Sironmani, S. Umapathi
    Abstract:

    To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443nm) when they were coated with BSA. The biointeractions of these engineered nanoparticles were studied using a mouse model. No significant changes in behavior or toxicity were observed. The nanoparticles were detected in all tissues including the Brain. Antibody recognition was monitored via the change in light absorption which accompanied binding, indicating that the particles can be used as a biosensor to gain more insight into cellular mechanisms governing the function of organs in general, and the blood Brain barrier (BBB) and Brain in particular.