The Experts below are selected from a list of 16452 Experts worldwide ranked by ideXlab platform
Neera Bhalla Sarin - One of the best experts on this subject based on the ideXlab platform.
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The Pollen Story of Brassica Juncea, Indian Mustard: In vitro Pollen Germination, Pollen Tube Growth and Viability Assessment
2018Co-Authors: Paomipem Phazang, Neera Bhalla SarinAbstract:The Pollen Story of Brassica Juncea, Indian Mustard: In vitro Pollen Germination, Pollen Tube Growth and Viability Assessment The Indian mustard, Brassica Juncea cv. Varuna is an annual oilseed crop in India. Because of its economical importance, this crop has gained a lot of interests among plant biologists. Advancements in science and technology have promoted multiple facet research that would aid in understanding and deciphering key elements that regulate growth and development in this crop. Contributing to the venture, this study highlights the pollen aspect of the Brassica Juncea plants. We succeeded in documenting pollen germination, pollen tube growth and viability in vitro in Brassica Juncea cv. Varuna. We established an efficient in vitro system that yielded >70% germination of Brasssica Juncea pollen grains and allowed normal growth and development of pollen tubes. The agarosebased substrate formulated not only served as matrix for pollen culture but also for microscopic analyses. In addition, it also served as suitable matrix for various dyes used, allowing easy and rapid staining for epifluorescence visualization. Flow cytometry (FCM) analyses gave rapid information about the viability of pollen grains. Conventional flouorochromatic reactions followed by microscopic examination could be avoided with FCM measurements.
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overexpression of γ tocopherol methyl transferase gene in transgenic Brassica Juncea plants alleviates abiotic stress physiological and chlorophyll a fluorescence measurements
Biochimica et Biophysica Acta, 2010Co-Authors: Mohd Aslam Yusuf, Deepak Kumar, Ravi Rajwanshi, Reto J Strasser, Merope Tsimillimichael, Neera Bhalla SarinAbstract:Abstract Tocopherols (vitamin E) are lipid soluble antioxidants synthesized by plants and some cyanobacteria. We have earlier reported that overexpression of the γ-tocopherol methyl transferase (γ-TMT) gene from Arabidopsis thaliana in transgenic Brassica Juncea plants resulted in an over six-fold increase in the level of α-tocopherol, the most active form of all the tocopherols. Tocopherol levels have been shown to increase in response to a variety of abiotic stresses. In the present study on Brassica Juncea, we found that salt, heavy metal and osmotic stress induced an increase in the total tocopherol levels. Measurements of seed germination, shoot growth and leaf disc senescence showed that transgenic Brassica Juncea plants overexpressing the γ-TMT gene had enhanced tolerance to the induced stresses. Analysis of the chlorophyll a fluorescence rise kinetics, from the initial “O” level to the “P” (the peak) level, showed that there were differential effects of the applied stresses on different sites of the photosynthetic machinery; further, these effects were alleviated in the transgenic (line 16.1) Brassica Juncea plants. We show that α-tocopherol plays an important role in the alleviation of stress induced by salt, heavy metal and osmoticum in Brassica Juncea.
Muhammad Yasir - One of the best experts on this subject based on the ideXlab platform.
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Indian Mustard Brassica Juncea efficiency for the accumulation, tolerance and translocation of zinc from metal contaminated soil
Biocatalysis and Agricultural Biotechnology, 2020Co-Authors: Hina Chaudhry, Numrah Nisar, Salma Mehmood, Munawar Iqbal, Arif Nazir, Muhammad YasirAbstract:Abstract Phytoremediation using hyper accumulators is an emerging and cost-effective option for heavy metal pollution cleanup in soils. There is a dearth of knowledge regarding mechanism of sequestration of heavy metals and their final destination allocated at cellular levels by hyper accumulators. The present study explores the accumulation, tolerance, toxicity, translocation and cellular level accumulation of essential heavy metal Zn in Indian Mustard (Brassica Juncea). Accumulation of Zn in roots leaves and stem of Brassica Juncea was also quantified by Atomic Absorption Spectroscopy (AAS). Plants were grown in sand and soil and were given Zn concentrations (20, 40, 80, and 160 mg/L) for four weeks. The results of this study showed that tolerance index increased with increased concentration of metal. Translocation factor for each concentration of Zn was greater than 1 which showed aerial parts of plants accumulated more amount of metal than their roots. Highest EC50 value was observed in stem and lowest EC50 value was observed in root of Brassica Juncea. Zn deposits were found in epidermis, bundle sheet of veins and mesophyll cells of Brassica Juncea leaves. Brassica Juncea tolerated and grew well even at higher concentrations of Zn. Zinc accumulation in leaf tissues increased as given concentration of Zn increased.
Mohd Aslam Yusuf - One of the best experts on this subject based on the ideXlab platform.
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overexpression of γ tocopherol methyl transferase gene in transgenic Brassica Juncea plants alleviates abiotic stress physiological and chlorophyll a fluorescence measurements
Biochimica et Biophysica Acta, 2010Co-Authors: Mohd Aslam Yusuf, Deepak Kumar, Ravi Rajwanshi, Reto J Strasser, Merope Tsimillimichael, Neera Bhalla SarinAbstract:Abstract Tocopherols (vitamin E) are lipid soluble antioxidants synthesized by plants and some cyanobacteria. We have earlier reported that overexpression of the γ-tocopherol methyl transferase (γ-TMT) gene from Arabidopsis thaliana in transgenic Brassica Juncea plants resulted in an over six-fold increase in the level of α-tocopherol, the most active form of all the tocopherols. Tocopherol levels have been shown to increase in response to a variety of abiotic stresses. In the present study on Brassica Juncea, we found that salt, heavy metal and osmotic stress induced an increase in the total tocopherol levels. Measurements of seed germination, shoot growth and leaf disc senescence showed that transgenic Brassica Juncea plants overexpressing the γ-TMT gene had enhanced tolerance to the induced stresses. Analysis of the chlorophyll a fluorescence rise kinetics, from the initial “O” level to the “P” (the peak) level, showed that there were differential effects of the applied stresses on different sites of the photosynthetic machinery; further, these effects were alleviated in the transgenic (line 16.1) Brassica Juncea plants. We show that α-tocopherol plays an important role in the alleviation of stress induced by salt, heavy metal and osmoticum in Brassica Juncea.
Hina Chaudhry - One of the best experts on this subject based on the ideXlab platform.
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Indian Mustard Brassica Juncea efficiency for the accumulation, tolerance and translocation of zinc from metal contaminated soil
Biocatalysis and Agricultural Biotechnology, 2020Co-Authors: Hina Chaudhry, Numrah Nisar, Salma Mehmood, Munawar Iqbal, Arif Nazir, Muhammad YasirAbstract:Abstract Phytoremediation using hyper accumulators is an emerging and cost-effective option for heavy metal pollution cleanup in soils. There is a dearth of knowledge regarding mechanism of sequestration of heavy metals and their final destination allocated at cellular levels by hyper accumulators. The present study explores the accumulation, tolerance, toxicity, translocation and cellular level accumulation of essential heavy metal Zn in Indian Mustard (Brassica Juncea). Accumulation of Zn in roots leaves and stem of Brassica Juncea was also quantified by Atomic Absorption Spectroscopy (AAS). Plants were grown in sand and soil and were given Zn concentrations (20, 40, 80, and 160 mg/L) for four weeks. The results of this study showed that tolerance index increased with increased concentration of metal. Translocation factor for each concentration of Zn was greater than 1 which showed aerial parts of plants accumulated more amount of metal than their roots. Highest EC50 value was observed in stem and lowest EC50 value was observed in root of Brassica Juncea. Zn deposits were found in epidermis, bundle sheet of veins and mesophyll cells of Brassica Juncea leaves. Brassica Juncea tolerated and grew well even at higher concentrations of Zn. Zinc accumulation in leaf tissues increased as given concentration of Zn increased.
Zengqiang Zhang - One of the best experts on this subject based on the ideXlab platform.
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role of streptomyces pactum in phytoremediation of trace elements by Brassica Juncea in mine polluted soils
Ecotoxicology and Environmental Safety, 2017Co-Authors: Amjad Ali, Di Guo, Amanullah Mahar, Zhen Wang, Dost Muhammad, Ping Wang, Feng Shen, Quanhong Xue, Zengqiang ZhangAbstract:The industrial expansion, smelting, mining and agricultural practices have increased the release of toxic trace elements (TEs) in the environment and threaten living organisms. The microbe-assisted phytoremediation is environmentally safe and provide an effective approach to remediate TEs contaminated soils. A pot experiment was conducted to test the potential of an Actinomycete, subspecies Streptomyces pactum (Act12) along with medical stone compost (MSC) by growing Brassica Juncea in smelter and mines polluted soils of Feng County (FC) and Tongguan (TG, China), respectively. Results showed that Zn (7, 28%), Pb (54, 21%), Cd (16, 17%) and Cu (8, 10%) uptake in shoot and root of Brassica Juncea was pronounced in FC soil. Meanwhile, the Zn (40, 14%) and Pb (82, 15%) uptake in the shoot and root were also increased in TG soil. Shoot Cd uptake remained below detection, while Cu decreased by 52% in TG soil. The Cd and Cu root uptake were increased by 17% and 33%, respectively. Results showed that TEs uptake in shoot increased with increasing Act12 dose. Shoot/root dry biomass, chlorophyll and carotenoid content in Brassica Juncea were significantly influenced by the application of Act12 in FC and TG soil. The antioxidant enzymatic activities (POD, PAL, PPO and CAT) in Brassica Juncea implicated enhancement in the plant defense mechanism against the TEs induced stress in contaminated soils. The extraction potential of Brasssica was further evaluated by TF (translocation factor) and MEA (metal extraction amount). Based on our findings, further investigation of Act12 assisted phytoremediation of TEs in the smelter and mines polluted soil and hyperaccumulator species are suggested for future studies.