The Experts below are selected from a list of 3285 Experts worldwide ranked by ideXlab platform
Philippa D Darbre - One of the best experts on this subject based on the ideXlab platform.
-
analysis of aluminium content and iron homeostasis in nipple aspirate fluids from healthy women and Breast cancer affected patients
Journal of Applied Toxicology, 2011Co-Authors: Ferdinando Mannello, Gaetana A Tonti, Virginia Medda, Patrizia Simone, Philippa D DarbreAbstract:Aluminium is not a physiological component of the Breast but has been measured recently in human Breast tissues and Breast cyst fluids at levels above those found in blood serum or milk. Since the presence of aluminium can lead to iron dyshomeostasis, levels of aluminium and iron-binding proteins (ferritin, transferrin) were measured in nipple aspirate fluid (NAF), a fluid present in the Breast Duct tree and mirroring the Breast microenvironment. NAFs were collected noninvasively from healthy women (NoCancer; n = 16) and Breast cancer-affected women (Cancer; n = 19), and compared with levels in serum (n = 15) and milk (n = 45) from healthy subjects. The mean level of aluminium, measured by ICP-mass spectrometry, was significantly higher in Cancer NAF (268.4 ± 28.1 μg l−1; n = 19) than in NoCancer NAF (131.3 ± 9.6 μg l−1; n = 16; P < 0.0001). The mean level of ferritin, measured through immunoassay, was also found to be higher in Cancer NAF (280.0 ± 32.3 μg l−1) than in NoCancer NAF (55.5 ± 7.2 μg l−1), and furthermore, a positive correlation was found between levels of aluminium and ferritin in the Cancer NAF (correlation coefficient R = 0.94, P < 0.001). These results may suggest a role for raised levels of aluminium and modulation of proteins that regulate iron homeostasis as biomarkers for identification of women at higher risk of developing Breast cancer. The reasons for the high levels of aluminium in NAF remain unknown but possibilities include either exposure to aluminium-based antiperspirant salts in the adjacent underarm area and/or preferential accumulation of aluminium by Breast tissues. Copyright © 2011 John Wiley & Sons, Ltd.
Allison W Kurian - One of the best experts on this subject based on the ideXlab platform.
-
magnetic resonance galactography a feasibility study in women with prior atypical Breast Duct cytology
Breast Journal, 2008Co-Authors: Allison W Kurian, Annerenee Hartman, Meredith A Mills, James M Ford, Laura Logan, Anne M Sawyer, Bruce L DanielAbstract:To the Editor: For women who inherit a high risk of developing Breast cancer, early detection is a key strategy to reduce morbidity and mortality (1). Multiple studies have found that standard mammographic screening often fails to detect Breast cancer early in women at high inherited risk of Breast cancer, prompting inclusion of emerging methods such as magnetic resonance imaging (MRI) into high-risk screening protocols (2– 13). The evaluation of Breast Duct cells is also under investigation as a potential cancer risk assessment technique. Approximately 80 percent of Breast cancers originate in the Ductal system, and early changes of the Ductal epithelium, which might predate malignant transformation, have been reported (14–16); for these reasons, analysis of Duct cells is a rational approach to risk stratification and biomarker development. At this time, understanding of the normal Breast Ductal anatomy, including an appreciation of variation in the number of Ducts per Breast, continues to evolve (17). A prior anatomic study on mastectomy specimens has also reported wide variation in the proportion of Breast volume drained by individual Ducts (18). With increasing study of intraDuctal approaches, a better comprehension of Ductal structure and the appearance of the Ductal system on MRI has practical relevance. We undertook a pilot study of a novel technique, magnetic resonance (MR) galactography, which combines MRI and intraDuctal evaluation. Our goals were to map the Breast Ductal system, quantify the Breast volume drained by individual Ducts, and evaluate sources of prior cytologic atypia in high-risk women.
-
Ductal lavage of fluid yielding and non fluid yielding Ducts in brca1 and brca2 mutation carriers and other women at high inherited Breast cancer risk
Cancer Epidemiology Biomarkers & Prevention, 2005Co-Authors: Allison W Kurian, Meredith A Mills, Sylvia K Plevritis, Margo Jaffee, Bronislava M Sigal, Nicolette M Chun, Kerry Kingham, Laura C Collins, Kent W Nowels, Judy GarberAbstract:Objective: Nipple fluid proDuction and atypical Breast Duct cells in women at high risk of Breast cancer have been associated with further increased risk. Most publications on Ductal lavage for cell collection report cannulating fluid-yielding Ducts only. We report lavage of fluid-yielding and non–fluid-yielding Ducts in women at high inherited Breast cancer risk. Methods: A pilot Breast cancer screening study including Ductal lavage was conDucted in 75 women at high inherited risk, 56 (74.7%) of whom had BRCA1/2 mutations. Ductal lavage was attempted in any Duct identifiable with a catheter. Results: Ducts were successfully catheterized in 60 of 75 patients (80%). Successfully catheterized patients were younger (median age 41 versus 53 years, P = 0.0003) and more often premenopausal (51.7% versus 20%, P = 0.041). Thirty-one successfully catheterized patients [51.6%, 95% confidence interval (39.4-63.9%)] had non–fluid-yielding Ducts only. Seventeen patients [28.3% (18.5-40.9%)] had atypical cells. Twelve of seventeen [70.6% (46.8-87.2%)] samples with atypia were from non–fluid-yielding Ducts. Patients with non–fluid-yielding Ducts (versus fluid-yielding Ducts) were more likely to have had prior cancer (48.4% versus 17.2%, P = 0.014) or chemotherapy (45.2% versus 17.2%, P = 0.027); this was also true in patients with atypia from non–fluid-yielding Ducts. Conclusion: Successfully lavaged women were younger and more often premenopausal. Atypical cells can be found in non–fluid-yielding Ducts in patients at high inherited Breast cancer risk. Non–fluid-yielding Ducts, and atypia from non–fluid-yielding Ducts, are more common in patients with prior cancer and chemotherapy. Larger studies are needed to identify risk factors and prognostic significance associated with atypia and non–fluid-yielding Ducts in high-risk populations, and define their role as biomarkers.
-
identification of Ductal atypia with mr galactography
Journal of Clinical Oncology, 2004Co-Authors: Annerenee Hartman, Meredith A Mills, Allison W Kurian, James M Ford, D N Smith, Bruce L DanielAbstract:1035 Background: Atypical Breast Duct cells in patients at increased risk of Breast cancer have been predictive of a 5-fold increased Breast cancer incidence. While Ductal lavage is an FDA approved procedure, very little is known about the anatomy of the Ductal system and when atypia is identified, how to localize the lesion when imaging tests are normal. The management of an atypical result is controversial and can include further evaluation in an attempt to localize a lesion with Ductoscopy and Breast MRI and risk reDuction measures including tamoxifen or prophylactic surgery. We report on the use of a combination of Breast MRI and Ductal lavage; MR-Galactography to help define the normal anatomy of the Breast Ductal system and to localize atypia not identified by mammography and MRI. Methods: Women who carry a BRCA1/2 mutation and have been undergoing annual screening with mammography, Breast MRI, and Ductal lavage in our centers were invited to participate. Ten microliters of a 5-mmol/L Gd-DTPA soluti...
-
Ductal lavage of non fluid yielding Ducts in brca1 and brca2 mutation carriers and other women at high genetic risk for Breast cancer
Journal of Clinical Oncology, 2004Co-Authors: Allison W Kurian, Meredith A Mills, James M Ford, Sylvia K Plevritis, Bronislava M Sigal, Nicolette M Chun, Kerry Kingham, Kent W Nowels, Annerenee HartmanAbstract:9535 Background: Atypical Breast Duct cells in patients at increased risk of Breast cancer have been predictive of subsequent Breast cancer. It has been reported that women whose Breasts yield no f...
Ferdinando Mannello - One of the best experts on this subject based on the ideXlab platform.
-
analysis of aluminium content and iron homeostasis in nipple aspirate fluids from healthy women and Breast cancer affected patients
Journal of Applied Toxicology, 2011Co-Authors: Ferdinando Mannello, Gaetana A Tonti, Virginia Medda, Patrizia Simone, Philippa D DarbreAbstract:Aluminium is not a physiological component of the Breast but has been measured recently in human Breast tissues and Breast cyst fluids at levels above those found in blood serum or milk. Since the presence of aluminium can lead to iron dyshomeostasis, levels of aluminium and iron-binding proteins (ferritin, transferrin) were measured in nipple aspirate fluid (NAF), a fluid present in the Breast Duct tree and mirroring the Breast microenvironment. NAFs were collected noninvasively from healthy women (NoCancer; n = 16) and Breast cancer-affected women (Cancer; n = 19), and compared with levels in serum (n = 15) and milk (n = 45) from healthy subjects. The mean level of aluminium, measured by ICP-mass spectrometry, was significantly higher in Cancer NAF (268.4 ± 28.1 μg l−1; n = 19) than in NoCancer NAF (131.3 ± 9.6 μg l−1; n = 16; P < 0.0001). The mean level of ferritin, measured through immunoassay, was also found to be higher in Cancer NAF (280.0 ± 32.3 μg l−1) than in NoCancer NAF (55.5 ± 7.2 μg l−1), and furthermore, a positive correlation was found between levels of aluminium and ferritin in the Cancer NAF (correlation coefficient R = 0.94, P < 0.001). These results may suggest a role for raised levels of aluminium and modulation of proteins that regulate iron homeostasis as biomarkers for identification of women at higher risk of developing Breast cancer. The reasons for the high levels of aluminium in NAF remain unknown but possibilities include either exposure to aluminium-based antiperspirant salts in the adjacent underarm area and/or preferential accumulation of aluminium by Breast tissues. Copyright © 2011 John Wiley & Sons, Ltd.
Annerenee Hartman - One of the best experts on this subject based on the ideXlab platform.
-
magnetic resonance galactography a feasibility study in women with prior atypical Breast Duct cytology
Breast Journal, 2008Co-Authors: Allison W Kurian, Annerenee Hartman, Meredith A Mills, James M Ford, Laura Logan, Anne M Sawyer, Bruce L DanielAbstract:To the Editor: For women who inherit a high risk of developing Breast cancer, early detection is a key strategy to reduce morbidity and mortality (1). Multiple studies have found that standard mammographic screening often fails to detect Breast cancer early in women at high inherited risk of Breast cancer, prompting inclusion of emerging methods such as magnetic resonance imaging (MRI) into high-risk screening protocols (2– 13). The evaluation of Breast Duct cells is also under investigation as a potential cancer risk assessment technique. Approximately 80 percent of Breast cancers originate in the Ductal system, and early changes of the Ductal epithelium, which might predate malignant transformation, have been reported (14–16); for these reasons, analysis of Duct cells is a rational approach to risk stratification and biomarker development. At this time, understanding of the normal Breast Ductal anatomy, including an appreciation of variation in the number of Ducts per Breast, continues to evolve (17). A prior anatomic study on mastectomy specimens has also reported wide variation in the proportion of Breast volume drained by individual Ducts (18). With increasing study of intraDuctal approaches, a better comprehension of Ductal structure and the appearance of the Ductal system on MRI has practical relevance. We undertook a pilot study of a novel technique, magnetic resonance (MR) galactography, which combines MRI and intraDuctal evaluation. Our goals were to map the Breast Ductal system, quantify the Breast volume drained by individual Ducts, and evaluate sources of prior cytologic atypia in high-risk women.
-
identification of Ductal atypia with mr galactography
Journal of Clinical Oncology, 2004Co-Authors: Annerenee Hartman, Meredith A Mills, Allison W Kurian, James M Ford, D N Smith, Bruce L DanielAbstract:1035 Background: Atypical Breast Duct cells in patients at increased risk of Breast cancer have been predictive of a 5-fold increased Breast cancer incidence. While Ductal lavage is an FDA approved procedure, very little is known about the anatomy of the Ductal system and when atypia is identified, how to localize the lesion when imaging tests are normal. The management of an atypical result is controversial and can include further evaluation in an attempt to localize a lesion with Ductoscopy and Breast MRI and risk reDuction measures including tamoxifen or prophylactic surgery. We report on the use of a combination of Breast MRI and Ductal lavage; MR-Galactography to help define the normal anatomy of the Breast Ductal system and to localize atypia not identified by mammography and MRI. Methods: Women who carry a BRCA1/2 mutation and have been undergoing annual screening with mammography, Breast MRI, and Ductal lavage in our centers were invited to participate. Ten microliters of a 5-mmol/L Gd-DTPA soluti...
-
Ductal lavage of non fluid yielding Ducts in brca1 and brca2 mutation carriers and other women at high genetic risk for Breast cancer
Journal of Clinical Oncology, 2004Co-Authors: Allison W Kurian, Meredith A Mills, James M Ford, Sylvia K Plevritis, Bronislava M Sigal, Nicolette M Chun, Kerry Kingham, Kent W Nowels, Annerenee HartmanAbstract:9535 Background: Atypical Breast Duct cells in patients at increased risk of Breast cancer have been predictive of subsequent Breast cancer. It has been reported that women whose Breasts yield no f...
Meredith A Mills - One of the best experts on this subject based on the ideXlab platform.
-
magnetic resonance galactography a feasibility study in women with prior atypical Breast Duct cytology
Breast Journal, 2008Co-Authors: Allison W Kurian, Annerenee Hartman, Meredith A Mills, James M Ford, Laura Logan, Anne M Sawyer, Bruce L DanielAbstract:To the Editor: For women who inherit a high risk of developing Breast cancer, early detection is a key strategy to reduce morbidity and mortality (1). Multiple studies have found that standard mammographic screening often fails to detect Breast cancer early in women at high inherited risk of Breast cancer, prompting inclusion of emerging methods such as magnetic resonance imaging (MRI) into high-risk screening protocols (2– 13). The evaluation of Breast Duct cells is also under investigation as a potential cancer risk assessment technique. Approximately 80 percent of Breast cancers originate in the Ductal system, and early changes of the Ductal epithelium, which might predate malignant transformation, have been reported (14–16); for these reasons, analysis of Duct cells is a rational approach to risk stratification and biomarker development. At this time, understanding of the normal Breast Ductal anatomy, including an appreciation of variation in the number of Ducts per Breast, continues to evolve (17). A prior anatomic study on mastectomy specimens has also reported wide variation in the proportion of Breast volume drained by individual Ducts (18). With increasing study of intraDuctal approaches, a better comprehension of Ductal structure and the appearance of the Ductal system on MRI has practical relevance. We undertook a pilot study of a novel technique, magnetic resonance (MR) galactography, which combines MRI and intraDuctal evaluation. Our goals were to map the Breast Ductal system, quantify the Breast volume drained by individual Ducts, and evaluate sources of prior cytologic atypia in high-risk women.
-
Ductal lavage of fluid yielding and non fluid yielding Ducts in brca1 and brca2 mutation carriers and other women at high inherited Breast cancer risk
Cancer Epidemiology Biomarkers & Prevention, 2005Co-Authors: Allison W Kurian, Meredith A Mills, Sylvia K Plevritis, Margo Jaffee, Bronislava M Sigal, Nicolette M Chun, Kerry Kingham, Laura C Collins, Kent W Nowels, Judy GarberAbstract:Objective: Nipple fluid proDuction and atypical Breast Duct cells in women at high risk of Breast cancer have been associated with further increased risk. Most publications on Ductal lavage for cell collection report cannulating fluid-yielding Ducts only. We report lavage of fluid-yielding and non–fluid-yielding Ducts in women at high inherited Breast cancer risk. Methods: A pilot Breast cancer screening study including Ductal lavage was conDucted in 75 women at high inherited risk, 56 (74.7%) of whom had BRCA1/2 mutations. Ductal lavage was attempted in any Duct identifiable with a catheter. Results: Ducts were successfully catheterized in 60 of 75 patients (80%). Successfully catheterized patients were younger (median age 41 versus 53 years, P = 0.0003) and more often premenopausal (51.7% versus 20%, P = 0.041). Thirty-one successfully catheterized patients [51.6%, 95% confidence interval (39.4-63.9%)] had non–fluid-yielding Ducts only. Seventeen patients [28.3% (18.5-40.9%)] had atypical cells. Twelve of seventeen [70.6% (46.8-87.2%)] samples with atypia were from non–fluid-yielding Ducts. Patients with non–fluid-yielding Ducts (versus fluid-yielding Ducts) were more likely to have had prior cancer (48.4% versus 17.2%, P = 0.014) or chemotherapy (45.2% versus 17.2%, P = 0.027); this was also true in patients with atypia from non–fluid-yielding Ducts. Conclusion: Successfully lavaged women were younger and more often premenopausal. Atypical cells can be found in non–fluid-yielding Ducts in patients at high inherited Breast cancer risk. Non–fluid-yielding Ducts, and atypia from non–fluid-yielding Ducts, are more common in patients with prior cancer and chemotherapy. Larger studies are needed to identify risk factors and prognostic significance associated with atypia and non–fluid-yielding Ducts in high-risk populations, and define their role as biomarkers.
-
identification of Ductal atypia with mr galactography
Journal of Clinical Oncology, 2004Co-Authors: Annerenee Hartman, Meredith A Mills, Allison W Kurian, James M Ford, D N Smith, Bruce L DanielAbstract:1035 Background: Atypical Breast Duct cells in patients at increased risk of Breast cancer have been predictive of a 5-fold increased Breast cancer incidence. While Ductal lavage is an FDA approved procedure, very little is known about the anatomy of the Ductal system and when atypia is identified, how to localize the lesion when imaging tests are normal. The management of an atypical result is controversial and can include further evaluation in an attempt to localize a lesion with Ductoscopy and Breast MRI and risk reDuction measures including tamoxifen or prophylactic surgery. We report on the use of a combination of Breast MRI and Ductal lavage; MR-Galactography to help define the normal anatomy of the Breast Ductal system and to localize atypia not identified by mammography and MRI. Methods: Women who carry a BRCA1/2 mutation and have been undergoing annual screening with mammography, Breast MRI, and Ductal lavage in our centers were invited to participate. Ten microliters of a 5-mmol/L Gd-DTPA soluti...
-
Ductal lavage of non fluid yielding Ducts in brca1 and brca2 mutation carriers and other women at high genetic risk for Breast cancer
Journal of Clinical Oncology, 2004Co-Authors: Allison W Kurian, Meredith A Mills, James M Ford, Sylvia K Plevritis, Bronislava M Sigal, Nicolette M Chun, Kerry Kingham, Kent W Nowels, Annerenee HartmanAbstract:9535 Background: Atypical Breast Duct cells in patients at increased risk of Breast cancer have been predictive of subsequent Breast cancer. It has been reported that women whose Breasts yield no f...