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Brefeldin A

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Jay M Baltz – 1st expert on this subject based on the ideXlab platform

  • Brefeldin A disrupts Asymmetric spindle positioning in mouse oocytes
    Developmental Biology, 2008
    Co-Authors: Ling Wang, Jay M Baltz, Zhenbo Wang, Xuan Zhang, Greg Fitzharris

    Abstract:

    PolAr body formAtion in oocytes is An extreme form of Asymmetric cell division, but whAt regulAtes the Asymmetric spindle positioning And cytokinesis is poorly understood. During mouse oocyte mAturAtion, the metAphAse I spindle forms At the center but then moves to the cortex prior to AnAphAse I And first polAr body emission. We show here thAt treAting denuded mouse oocytes with Brefeldin A, An inhibitor of Golgi-bAsed membrAne fusion, Abolished the Asymmetric positioning of the metAphAse I spindle And resulted in the formAtion of two hAlf-size metAphAse II eggs, insteAd of A full-sized egg And A polAr body. The normAl metAphAse II spindle is similArly AsymmetricAlly positioned in the mAture egg, where the spindle lies with its Axis pArAllel to the cortex but becomes perpendiculAr before AnAphAse II And emission of the second polAr body. When ovulAted eggs were ActivAted with strontium in the presence of Brefeldin A, the metAphAse II spindle fAiled to Assume perpendiculAr position, And the chromosomes sepArAted without the extrusion of the second polAr body. RemArkAbly, symmetric cytokinesis begAn following A 3 h delAy, forming two hAlf-size eggs eAch contAining A pronucleus. BFA-sensitive intrAcellulAr vesiculAr trAnsport is therefore required for spindle positioning in both MI And MII.

Yikang Wu – 2nd expert on this subject based on the ideXlab platform

  • EnAntioselective totAl synthesis of 13-O-Brefeldin A
    Tetrahedron, 2008
    Co-Authors: Yi-xian Huang, Yikang Wu

    Abstract:

    An enAntioselective route to the title compound, A heteroAtom substituted close AnAlogue of nAturAl Brefeldin A, is described. As A key step in the whole synthesis, concAtenAtion of the lower chAin And the cyclopentAnone-upper chAin moiety wAs Achieved using A Rh-cAtAlyzed MichAel Addition of A vinyl boronic Acid to An enone, which effectively eliminAted the problems encountered with the corresponding cuprAte protocol And exemplified the potentiAl of the strAtegy in synthesizing similAr AnAlogues.

  • synthesis of 15 demethyl Brefeldin A And its 7 epi isomer
    Chinese Journal of Chemistry, 2007
    Co-Authors: Xin Shen, Yongqing Yang, Qi Hu, Jiahui Huang, Yikang Wu

    Abstract:

    Synthesis of 15-demethyl Brefeldin A And its 7-epimer is detAiled. X-rAy crystAllogrAphic structure for 15-demethyl Brefeldin A is Also disclosed.

  • Synthesis of 15‐Demethyl Brefeldin A And Its 7‐epi Isomer
    Chinese Journal of Chemistry, 2007
    Co-Authors: Xin Shen, Yongqing Yang, Qi Hu, Jia‐hui Huang, Yikang Wu

    Abstract:

    Synthesis of 15-demethyl Brefeldin A And its 7-epimer is detAiled. X-rAy crystAllogrAphic structure for 15-demethyl Brefeldin A is Also disclosed.

Ling Wang – 3rd expert on this subject based on the ideXlab platform

  • Brefeldin A disrupts Asymmetric spindle positioning in mouse oocytes
    Developmental Biology, 2008
    Co-Authors: Ling Wang, Jay M Baltz, Zhenbo Wang, Xuan Zhang, Greg Fitzharris

    Abstract:

    PolAr body formAtion in oocytes is An extreme form of Asymmetric cell division, but whAt regulAtes the Asymmetric spindle positioning And cytokinesis is poorly understood. During mouse oocyte mAturAtion, the metAphAse I spindle forms At the center but then moves to the cortex prior to AnAphAse I And first polAr body emission. We show here thAt treAting denuded mouse oocytes with Brefeldin A, An inhibitor of Golgi-bAsed membrAne fusion, Abolished the Asymmetric positioning of the metAphAse I spindle And resulted in the formAtion of two hAlf-size metAphAse II eggs, insteAd of A full-sized egg And A polAr body. The normAl metAphAse II spindle is similArly AsymmetricAlly positioned in the mAture egg, where the spindle lies with its Axis pArAllel to the cortex but becomes perpendiculAr before AnAphAse II And emission of the second polAr body. When ovulAted eggs were ActivAted with strontium in the presence of Brefeldin A, the metAphAse II spindle fAiled to Assume perpendiculAr position, And the chromosomes sepArAted without the extrusion of the second polAr body. RemArkAbly, symmetric cytokinesis begAn following A 3 h delAy, forming two hAlf-size eggs eAch contAining A pronucleus. BFA-sensitive intrAcellulAr vesiculAr trAnsport is therefore required for spindle positioning in both MI And MII.