Bromcresol Purple

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Mitsutaka Yoshida - One of the best experts on this subject based on the ideXlab platform.

  • effects of serum isolated vs synthetic bilirubin albumin complexes on dye binding methods for estimating serum albumin
    Clinical Chemistry, 1991
    Co-Authors: Hiroshi Ihara, Hiroomi Nakamura, Yutaka Aoki, Tsugutoshi Aoki, Mitsutaka Yoshida
    Abstract:

    Bush and Reed reported (Clin Chem 1987;33:821-3) that the reaction of albumin with Bromcresol Purple but not with Bromcresol green underestimated the concentration of albumin in synthetically obtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indistinguishable from the natural Bd in icteric serum. Here we verify that Bd, whether synthetically obtained or isolated from serum, causes an underestimation of albumin in the Bromcresol Purple but not in the Bromcresol green method. The molar ratio of Bd from either source to underestimated albumin approximates 1.0, suggesting that one molecule of Bd would react equivalently to a molecule of albumin in the Bromcresol Purple method. This underestimation might falsely suggest hypoalbuminemia in patients with increased serum Bd.

  • Effects of serum-isolated vs. synthetic bilirubin-albumin complexes on dyebinding methods for estimating serum albumin Clin Chem
    1991
    Co-Authors: Hiroshi Ihara, Hiroomi Nakamura, Yutaka Aoki, Tsugutoshi Aoki, Mitsutaka Yoshida
    Abstract:

    the reaction of albumin with Bromcresol Purple but not with Bromcresol green underestimated the concentration of albumin in syntheticallyobtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indistinguishable from the natural Bd in icteric serum. Here we verify that Bd, whether syntheticallyobtained or isolatedfrom serum, causes an underestimation of albumin in the Bromcresol Purple but not in the Bromcresol green method. The molar ratio of Bd from either source to underestimated albumin approximates 1.0, suggesting that one molecule of Bd would react equivalently to a molecule of albumin in the Bromcresol Purple method. This underestimation might falsely suggest hypoalbuminemia in patients with in-creased serum Bd

Macit Ilkit - One of the best experts on this subject based on the ideXlab platform.

  • diagnostic value of morphological physiological and biochemical tests in distinguishing trichophyton rubrum from trichophyton mentagrophytes complex
    Medical Mycology, 2008
    Co-Authors: Ayli N Ates, Kadri Ozcan, Macit Ilkit
    Abstract:

    The two most frequently encountered dermatophyte etiologic agents of glabrous skin and nail dermatophytoses are Trichophyton rubrum and T. mentagrophytes. This study was aimed to discuss the efficacy of morphological, physiological and biochemical diagnostic tests commonly used in the identification of T. rubrum and members of the T. mentagrophytes complex. In this study, we evaluated; hydrolysis of urea in broth and on urea agar slants and Petri plates incubated at 228C, 288C and 378C, in vitro hair perforation (blond child, sheep and goat hair), pigment production on cornmeal dextrose agar (CMDA) and Bromcresol Purple-milk solids-glucose agar (BCP-MS-G), Tween opacity, sorbitol assimilation, and salt tolerance. Additionally, the production of micro- and macroconidia was investigated by using brain heart infusion agar (BHIA), Christensen’s urea agar in Petri plates (UPA), CMDA, Lowenstein-Jensen agar (LJA), malt extract agar, oatmeal agar, Oxoid chromogenic Candida agar, and potato dextrose agar. All cultures were incubated at 288C, and conidial production was compared on days 5, 10 and 15. It was found that the urea hydrolysis test yielded more rapid and significant results when urea medium was prepared in Petri plates and incubated at 288 C( PB0.01). LJA supported the highest production of microconidia after 15 days (P B0.001). Additionally, it was found that T. rubrum strains produced red pigment on CMDA (PB0.01) and BCP-MS-G, while strains of the T. mentagrophytes species complex did not. A special algorithm containing the various test procedures employed in these studies is presented which was found to be useful in the differentiation of T. rubrum strains from T. mentagrophytes complex. Our results revealed that UPA, CMDA, BCP-MS-G, LJA, and BHIA may be used as common mycological agars in routine practice.

Hiroshi Ihara - One of the best experts on this subject based on the ideXlab platform.

  • effects of serum isolated vs synthetic bilirubin albumin complexes on dye binding methods for estimating serum albumin
    Clinical Chemistry, 1991
    Co-Authors: Hiroshi Ihara, Hiroomi Nakamura, Yutaka Aoki, Tsugutoshi Aoki, Mitsutaka Yoshida
    Abstract:

    Bush and Reed reported (Clin Chem 1987;33:821-3) that the reaction of albumin with Bromcresol Purple but not with Bromcresol green underestimated the concentration of albumin in synthetically obtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indistinguishable from the natural Bd in icteric serum. Here we verify that Bd, whether synthetically obtained or isolated from serum, causes an underestimation of albumin in the Bromcresol Purple but not in the Bromcresol green method. The molar ratio of Bd from either source to underestimated albumin approximates 1.0, suggesting that one molecule of Bd would react equivalently to a molecule of albumin in the Bromcresol Purple method. This underestimation might falsely suggest hypoalbuminemia in patients with increased serum Bd.

  • Effects of serum-isolated vs. synthetic bilirubin-albumin complexes on dyebinding methods for estimating serum albumin Clin Chem
    1991
    Co-Authors: Hiroshi Ihara, Hiroomi Nakamura, Yutaka Aoki, Tsugutoshi Aoki, Mitsutaka Yoshida
    Abstract:

    the reaction of albumin with Bromcresol Purple but not with Bromcresol green underestimated the concentration of albumin in syntheticallyobtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indistinguishable from the natural Bd in icteric serum. Here we verify that Bd, whether syntheticallyobtained or isolatedfrom serum, causes an underestimation of albumin in the Bromcresol Purple but not in the Bromcresol green method. The molar ratio of Bd from either source to underestimated albumin approximates 1.0, suggesting that one molecule of Bd would react equivalently to a molecule of albumin in the Bromcresol Purple method. This underestimation might falsely suggest hypoalbuminemia in patients with in-creased serum Bd

Ayli N Ates - One of the best experts on this subject based on the ideXlab platform.

  • diagnostic value of morphological physiological and biochemical tests in distinguishing trichophyton rubrum from trichophyton mentagrophytes complex
    Medical Mycology, 2008
    Co-Authors: Ayli N Ates, Kadri Ozcan, Macit Ilkit
    Abstract:

    The two most frequently encountered dermatophyte etiologic agents of glabrous skin and nail dermatophytoses are Trichophyton rubrum and T. mentagrophytes. This study was aimed to discuss the efficacy of morphological, physiological and biochemical diagnostic tests commonly used in the identification of T. rubrum and members of the T. mentagrophytes complex. In this study, we evaluated; hydrolysis of urea in broth and on urea agar slants and Petri plates incubated at 228C, 288C and 378C, in vitro hair perforation (blond child, sheep and goat hair), pigment production on cornmeal dextrose agar (CMDA) and Bromcresol Purple-milk solids-glucose agar (BCP-MS-G), Tween opacity, sorbitol assimilation, and salt tolerance. Additionally, the production of micro- and macroconidia was investigated by using brain heart infusion agar (BHIA), Christensen’s urea agar in Petri plates (UPA), CMDA, Lowenstein-Jensen agar (LJA), malt extract agar, oatmeal agar, Oxoid chromogenic Candida agar, and potato dextrose agar. All cultures were incubated at 288C, and conidial production was compared on days 5, 10 and 15. It was found that the urea hydrolysis test yielded more rapid and significant results when urea medium was prepared in Petri plates and incubated at 288 C( PB0.01). LJA supported the highest production of microconidia after 15 days (P B0.001). Additionally, it was found that T. rubrum strains produced red pigment on CMDA (PB0.01) and BCP-MS-G, while strains of the T. mentagrophytes species complex did not. A special algorithm containing the various test procedures employed in these studies is presented which was found to be useful in the differentiation of T. rubrum strains from T. mentagrophytes complex. Our results revealed that UPA, CMDA, BCP-MS-G, LJA, and BHIA may be used as common mycological agars in routine practice.

Hiroomi Nakamura - One of the best experts on this subject based on the ideXlab platform.

  • effects of serum isolated vs synthetic bilirubin albumin complexes on dye binding methods for estimating serum albumin
    Clinical Chemistry, 1991
    Co-Authors: Hiroshi Ihara, Hiroomi Nakamura, Yutaka Aoki, Tsugutoshi Aoki, Mitsutaka Yoshida
    Abstract:

    Bush and Reed reported (Clin Chem 1987;33:821-3) that the reaction of albumin with Bromcresol Purple but not with Bromcresol green underestimated the concentration of albumin in synthetically obtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indistinguishable from the natural Bd in icteric serum. Here we verify that Bd, whether synthetically obtained or isolated from serum, causes an underestimation of albumin in the Bromcresol Purple but not in the Bromcresol green method. The molar ratio of Bd from either source to underestimated albumin approximates 1.0, suggesting that one molecule of Bd would react equivalently to a molecule of albumin in the Bromcresol Purple method. This underestimation might falsely suggest hypoalbuminemia in patients with increased serum Bd.

  • Effects of serum-isolated vs. synthetic bilirubin-albumin complexes on dyebinding methods for estimating serum albumin Clin Chem
    1991
    Co-Authors: Hiroshi Ihara, Hiroomi Nakamura, Yutaka Aoki, Tsugutoshi Aoki, Mitsutaka Yoshida
    Abstract:

    the reaction of albumin with Bromcresol Purple but not with Bromcresol green underestimated the concentration of albumin in syntheticallyobtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indistinguishable from the natural Bd in icteric serum. Here we verify that Bd, whether syntheticallyobtained or isolatedfrom serum, causes an underestimation of albumin in the Bromcresol Purple but not in the Bromcresol green method. The molar ratio of Bd from either source to underestimated albumin approximates 1.0, suggesting that one molecule of Bd would react equivalently to a molecule of albumin in the Bromcresol Purple method. This underestimation might falsely suggest hypoalbuminemia in patients with in-creased serum Bd

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