The Experts below are selected from a list of 12405 Experts worldwide ranked by ideXlab platform
Kerstin Schweyer - One of the best experts on this subject based on the ideXlab platform.
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neuronal precursor cells with dopaminergic commitment in the rostral migratory stream of the mouse
Scientific Reports, 2019Co-Authors: Kerstin Schweyer, Corinna Ruschoffsteiner, Oscar Ariascarrion, Thomas W Rosler, Wolfgang H Oertel, Gunter U HoglingerAbstract:Neuroblasts born in the subventricular zone of adult mammals migrate via the rostral migratory stream into the granular cell layer or periglomerular layer of the olfactory bulb to differentiate into interneurons. To analyze if new neurons in the granular cell layer or periglomerular layer have different origins, we inserted a physical barrier into the rostral migratory stream, depleted cell proliferation with cytarabine infusions, labeled newborn cells with Bromodeoxyuridine, and sacrificed mice after short-term (0, 2, or 14 days) or long-term (55 or 105 days) intervals. After short-term survival, the subventricular zone and rostral migratory stream rapidly repopulated with Bromodeoxyuridine+ cells after cytarabine-induced depletion. Nestin, glial fibrillary acidic protein and the PAX6 were expressed in Bromodeoxyuridine+ cells within the rostral migratory stream downstream of the physical barrier. After long-term survival after physical barrier implantation, Bromodeoxyuridine+ neurons were significantly reduced in the granular cell layer, but Bromodeoxyuridine+ and dopaminergic neurons in the periglomerular layer remained unaffected by the physical barrier. Thus, newborn neurons for the granular cell layer are mainly recruited from neural stem cells located in the subventricular zone, but new neurons for the periglomerular layer with dopaminergic predisposition can rise as well from neuronal stem or precursor cells in the rostral migratory stream.
Bente Finsen - One of the best experts on this subject based on the ideXlab platform.
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axonal plasticity elicits long term changes in oligodendroglia and myelinated fibers
Glia, 2010Co-Authors: Nina Drojdahl, Helle Hvilsted Nielsen, J E Gardi, Andreas Wree, Alan C Peterson, Jens R Nyengaard, Joel Eyer, Bente FinsenAbstract:Axons are linked to induction of myelination during development and to the maintenance of myelin and myelinated tracts in the adult CNS. Currently, it is unknown whether and how axonal plasticity in adult CNS impacts the myelinating cells and their precursors. In this article, we report that newly formed axonal sprouts are able to induce a protracted myelination response in adult CNS. We show that newly formed axonal sprouts, induced by lesion of the entorhino-hippocampal perforant pathway, have the ability to induce a myelination response in stratum radiatum and lucidum CA3. The lesion resulted in significant recruitment of newly formed myelinating cells, documented by incorporation of the proliferation marker Bromodeoxyuridine into chondroitin sulphate NG2 expressing cells in stratum radiatum and lucidum CA3 early after lesion, and the occurrence of a 28% increase in the number of oligodendrocytes, of which some had incorporated Bromodeoxyuridine, 9 weeks post-lesion. Additionally, a marked increase (41%) in myelinated fibres was detected in silver stained sections. Interestingly, these apparently new fibres achieved the same axon diameter as unlesioned mice but myelin thickness remained thinner than normal, suggesting that the sprouting axons in stratum radiatum and lucidum CA3 were not fully myelinated 9 weeks after lesion. Our combined results show that sprouting axons provide a strong stimulus to oligodendrocyte lineage cells to engage actively in the myelination processes in the adult CNS.
Eisuke Gotoh - One of the best experts on this subject based on the ideXlab platform.
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a method for detecting sister chromatid exchanges using prematurely condensed chromosomes and immunogold silver staining
Mutagenesis, 1997Co-Authors: Yohko Asakawa, Eisuke GotohAbstract:We have developed a method for detecting sister chromatid exchanges using premature chromosome condensation and immunogold labelling followed by silver enhancement. Cells were grown in Bromodeoxyuridine before sister chromatid exchanges were generated with mitomycin C. Calyculin A was then used to condense the chromosomes prematurely. Finally, incorporated Bromodeoxyuridine was detected using a gold-conjugated antibody followed by silver enhancement. Exchanged chromatids could easily be seen in the resulting chromosomes. This simple method allows sister chromatid exchanges to be detected with great sensitivity and resolution.
P Fernandezllebrez - One of the best experts on this subject based on the ideXlab platform.
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neurogenesis in explants from the walls of the lateral ventricle of adult bovine brain role of endogenous igf 1 as a survival factor
European Journal of Neuroscience, 2003Co-Authors: Margarita Perezmartin, Manuel Cifuentes, J M Grondona, Francisco Javier Bermudezsilva, Pilar M Arrabal, J M Perezfigares, Antonio J Jimenez, Luis M Garciasegura, P FernandezllebrezAbstract:Previous studies have shown the existence of proliferating cells in explants from bovine (Bos Taurus) lateral ventricle walls that were maintained for several days in vitro in the absence of serum and growth factors. In this study we have characterized the nature of new cells and have assessed whether the insulin-like growth factor-1 (IGF-1) receptor regulates their survival and/or proliferation. The explants were composed of the ependymal layer and attached subependymal cells. Ependymal cells in culture were labelled with glial markers (S-100, vimentin, GFAP, BLBP, 3A7 and 3CB2) and did not incorporate bromodeoxiuridine when this molecule was added to the culture media. Most subependymal cells were immunoreactive for beta III-tubulin, a neuronal marker, and did incorporate bromodeoxiuridine. Subependymal neurons displayed immunoreactivity for IGF-1 and its receptor and expressed IGF-1 mRNA, indicating that IGF-1 is produced in the explants and may act on new neurons. Addition to the culture media of an IGF-1 receptor antagonist, the peptide JB1, did not affect the incorporation of bromodeoxiuridine to proliferating subependymal cells. However, JB1 significantly increased the number of TUNEL positive cells in the subependymal zone, suggesting that IGF-1 receptor is involved in the survival of subependymal neurons. In conclusion, these findings indicate that neurogenesis is maintained in explants from the lateral cerebral ventricle of adult bovine brains and that IGF-1 is locally produced in the explants and may regulate the survival of the proliferating neurons.
Gunter U Hoglinger - One of the best experts on this subject based on the ideXlab platform.
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neuronal precursor cells with dopaminergic commitment in the rostral migratory stream of the mouse
Scientific Reports, 2019Co-Authors: Kerstin Schweyer, Corinna Ruschoffsteiner, Oscar Ariascarrion, Thomas W Rosler, Wolfgang H Oertel, Gunter U HoglingerAbstract:Neuroblasts born in the subventricular zone of adult mammals migrate via the rostral migratory stream into the granular cell layer or periglomerular layer of the olfactory bulb to differentiate into interneurons. To analyze if new neurons in the granular cell layer or periglomerular layer have different origins, we inserted a physical barrier into the rostral migratory stream, depleted cell proliferation with cytarabine infusions, labeled newborn cells with Bromodeoxyuridine, and sacrificed mice after short-term (0, 2, or 14 days) or long-term (55 or 105 days) intervals. After short-term survival, the subventricular zone and rostral migratory stream rapidly repopulated with Bromodeoxyuridine+ cells after cytarabine-induced depletion. Nestin, glial fibrillary acidic protein and the PAX6 were expressed in Bromodeoxyuridine+ cells within the rostral migratory stream downstream of the physical barrier. After long-term survival after physical barrier implantation, Bromodeoxyuridine+ neurons were significantly reduced in the granular cell layer, but Bromodeoxyuridine+ and dopaminergic neurons in the periglomerular layer remained unaffected by the physical barrier. Thus, newborn neurons for the granular cell layer are mainly recruited from neural stem cells located in the subventricular zone, but new neurons for the periglomerular layer with dopaminergic predisposition can rise as well from neuronal stem or precursor cells in the rostral migratory stream.
