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Jaques Belik - One of the best experts on this subject based on the ideXlab platform.
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Bronchial Muscle Peristaltic Activity in the Fetal Rat
Pediatric Research, 2006Co-Authors: Omar Parvez, Anne-marie Voss, Mascha De Kok, Matthias Roth-kleiner, Jaques BelikAbstract:Aside for the potential for tonic contraction, the airway smooth Muscle exhibits intermittent phasic rhythmic activity that may contribute to lung growth during fetal life. Therefore, we examined 4th generation rat 18–22 d gestation fetal, 4–6 d of age newborn and adult Bronchial ring from Sprague Dawley rats to compare differences in smooth Muscle function. We hypothesized that phasic contractions were greatest before birth. Bronchial Muscle spontaneous rhythmic contractions were greatest in the fetus and absent in the adult. In response to KCl stimulation, the fetal Bronchial smooth Muscle only developed tonic force that was 3.5 ± 0.6 and lower than measured in the newborn 9.0 ± 0.3 and adult 13.7 ± 1.4mN/mm^2. The thromboxane A_2 analogue U46619 induced tonic and phasic Muscle contractions and the amplitude and frequency of the phasic contractions were greater in the fetus as compared with the adult and increased with gestational age. The U46619-induced rhythmic contractions were abrogated by ryanodine, thapsigargin and reduction of extracellular Na^+, suggesting intracellular Ca^2+ dependence and involvement of the Na^+/Ca^2+ exchanger. The inward rectifier K^+ blocker BaCl_2 induced phasic contractions in unstimulated fetal, but not adult Bronchial Muscle of the same amplitude and frequency as for the spontaneous and U46619-induced ones. We conclude that the airway smooth Muscle phasic activity is greatest in the fetus and tends to disappear post-natally with age suggesting an in utero role during lung development.
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articles Bronchial Muscle peristaltic activity in the fetal rat
2006Co-Authors: Omar Parvez, Anne-marie Voss, Mascha De Kok, Matthias Rothkleiner, Jaques BelikAbstract:Aside for the potential for tonic contraction, the airway smooth Muscle exhibits intermittent phasic rhythmic activity that may contribute to lung growth during fetal life. Therefore, we examined 4th generation rat 18 -22 d gestation fetal, 4 - 6 d of age newborn and adult Bronchial ring from Sprague Dawley rats to compare differences in smooth Muscle function. We hypothesized that phasic contractions were greatest before birth. Bronchial Muscle spontaneous rhythmic contractions were greatest in the fetus and absent in the adult. In response to KCl stimulation, the fetal Bronchial smooth Muscle only developed tonic force that was 3.5 0.6 and lower than measured in the newborn 9.0 0.3 and adult 13.7 1.4mN/mm 2 . The thromboxane A2 analogue U46619 induced tonic and phasic Muscle contractions and the amplitude and frequency of the phasic contractions were greater in the fetus as compared with the adult and increased with gestational age. The U46619-induced rhyth- mic contractions were abrogated by ryanodine, thapsigargin and reduction of extracellular Na, suggesting intracellular Ca 2 depen- dence and involvement of the Na/Ca 2 exchanger. The inward rectifier K blocker BaCl2 induced phasic contractions in unstimu- lated fetal, but not adult Bronchial Muscle of the same amplitude and frequency as for the spontaneous and U46619-induced ones. We conclude that the airway smooth Muscle phasic activity is greatest in the fetus and tends to disappear post-natally with age suggesting an in utero role during lung development. (Pediatr Res 59: 756-761, 2006)
Ana Paula Ligeiro De Oliveira - One of the best experts on this subject based on the ideXlab platform.
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low level laser therapy inhibits bronchoconstriction th2 inflammation and airway remodeling in allergic asthma
Respiratory Physiology & Neurobiology, 2014Co-Authors: Vanessa Silva, P Marcondes, Morgana Duarte Da Silva, Antonio Balbin Villaverde, Hugo C Castrofarianeto, Rodolfo De Paula Vieira, Flavio Aimbire, Ana Paula Ligeiro De OliveiraAbstract:Abstract Low-level laser therapy (LLLT) controls Bronchial hyperresponsiveness (BHR) associated with increased RhoA expression as well as pro-inflammatory mediators associated with NF-kB in acute lung inflammation. Herein, we explore if LLLT can reduce both BHR and Th2 cytokines in allergic asthma. Mice were studied for Bronchial reactivity and lung inflammation after antigen challenge. BHR was measured through dose–response curves to acetylcholine. Some animals were pretreated with a RhoA inhibitor before the antigen. LLLT (660 nm, 30 mW and 5.4 J) was applied on the skin over the right upper bronchus and two irradiation protocols were used. Reduction of BHR post LLLT coincided with lower RhoA expression in Bronchial Muscle as well as reduction in eosinophils and eotaxin. LLLT also diminished ICAM expression and Th2 cytokines as well as signal transducer and activator of transduction 6 (STAT6) levels in lungs from challenged mice. Our results demonstrated that LLLT reduced BHR via RhoA and lessened allergic lung inflammation via STAT6.
Chenghan Lee - One of the best experts on this subject based on the ideXlab platform.
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acetylcholine induced asynchronous calcium waves in intact human Bronchial Muscle bundle
American Journal of Respiratory Cell and Molecular Biology, 2007Co-Authors: J Dai, Kuohsing Kuo, Joyce M Leo, Peter D Pare, Cornelis Van Breemen, Chenghan LeeAbstract:Calcium (Ca2+) is an important activator of the contractile machinery in airway smooth Muscle (ASM). While agonist-induced Ca2+ signals are well characterized in animal ASM, little is known about what occurs in adult human ASM. In this study, we examined the Ca2+ signal elicited by acetylcholine (ACh) in smooth Muscle cells of the intact human Bronchial Muscle strips obtained from fresh surgical specimens in relation to Muscle contraction. We found that ACh induces repetitive Ca2+ waves that spread along the longitudinal axis of individual cells in the intact human Bronchial smooth Muscle strips. These Ca2+ waves display no apparent synchronization between neighboring cells, and their generation precedes force development. Comparison of the ACh concentration dependence of tissue contraction and selected parameters of the asynchronous Ca2+ waves (ACW) reveals that the graded force generation by ACh-stimulated human Bronchial Muscle strips is achieved by differential recruitment of cells to initiate Ca2+ wa...
Omar Parvez - One of the best experts on this subject based on the ideXlab platform.
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Bronchial Muscle Peristaltic Activity in the Fetal Rat
Pediatric Research, 2006Co-Authors: Omar Parvez, Anne-marie Voss, Mascha De Kok, Matthias Roth-kleiner, Jaques BelikAbstract:Aside for the potential for tonic contraction, the airway smooth Muscle exhibits intermittent phasic rhythmic activity that may contribute to lung growth during fetal life. Therefore, we examined 4th generation rat 18–22 d gestation fetal, 4–6 d of age newborn and adult Bronchial ring from Sprague Dawley rats to compare differences in smooth Muscle function. We hypothesized that phasic contractions were greatest before birth. Bronchial Muscle spontaneous rhythmic contractions were greatest in the fetus and absent in the adult. In response to KCl stimulation, the fetal Bronchial smooth Muscle only developed tonic force that was 3.5 ± 0.6 and lower than measured in the newborn 9.0 ± 0.3 and adult 13.7 ± 1.4mN/mm^2. The thromboxane A_2 analogue U46619 induced tonic and phasic Muscle contractions and the amplitude and frequency of the phasic contractions were greater in the fetus as compared with the adult and increased with gestational age. The U46619-induced rhythmic contractions were abrogated by ryanodine, thapsigargin and reduction of extracellular Na^+, suggesting intracellular Ca^2+ dependence and involvement of the Na^+/Ca^2+ exchanger. The inward rectifier K^+ blocker BaCl_2 induced phasic contractions in unstimulated fetal, but not adult Bronchial Muscle of the same amplitude and frequency as for the spontaneous and U46619-induced ones. We conclude that the airway smooth Muscle phasic activity is greatest in the fetus and tends to disappear post-natally with age suggesting an in utero role during lung development.
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articles Bronchial Muscle peristaltic activity in the fetal rat
2006Co-Authors: Omar Parvez, Anne-marie Voss, Mascha De Kok, Matthias Rothkleiner, Jaques BelikAbstract:Aside for the potential for tonic contraction, the airway smooth Muscle exhibits intermittent phasic rhythmic activity that may contribute to lung growth during fetal life. Therefore, we examined 4th generation rat 18 -22 d gestation fetal, 4 - 6 d of age newborn and adult Bronchial ring from Sprague Dawley rats to compare differences in smooth Muscle function. We hypothesized that phasic contractions were greatest before birth. Bronchial Muscle spontaneous rhythmic contractions were greatest in the fetus and absent in the adult. In response to KCl stimulation, the fetal Bronchial smooth Muscle only developed tonic force that was 3.5 0.6 and lower than measured in the newborn 9.0 0.3 and adult 13.7 1.4mN/mm 2 . The thromboxane A2 analogue U46619 induced tonic and phasic Muscle contractions and the amplitude and frequency of the phasic contractions were greater in the fetus as compared with the adult and increased with gestational age. The U46619-induced rhyth- mic contractions were abrogated by ryanodine, thapsigargin and reduction of extracellular Na, suggesting intracellular Ca 2 depen- dence and involvement of the Na/Ca 2 exchanger. The inward rectifier K blocker BaCl2 induced phasic contractions in unstimu- lated fetal, but not adult Bronchial Muscle of the same amplitude and frequency as for the spontaneous and U46619-induced ones. We conclude that the airway smooth Muscle phasic activity is greatest in the fetus and tends to disappear post-natally with age suggesting an in utero role during lung development. (Pediatr Res 59: 756-761, 2006)
Vanessa Silva - One of the best experts on this subject based on the ideXlab platform.
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low level laser therapy inhibits bronchoconstriction th2 inflammation and airway remodeling in allergic asthma
Respiratory Physiology & Neurobiology, 2014Co-Authors: Vanessa Silva, P Marcondes, Morgana Duarte Da Silva, Antonio Balbin Villaverde, Hugo C Castrofarianeto, Rodolfo De Paula Vieira, Flavio Aimbire, Ana Paula Ligeiro De OliveiraAbstract:Abstract Low-level laser therapy (LLLT) controls Bronchial hyperresponsiveness (BHR) associated with increased RhoA expression as well as pro-inflammatory mediators associated with NF-kB in acute lung inflammation. Herein, we explore if LLLT can reduce both BHR and Th2 cytokines in allergic asthma. Mice were studied for Bronchial reactivity and lung inflammation after antigen challenge. BHR was measured through dose–response curves to acetylcholine. Some animals were pretreated with a RhoA inhibitor before the antigen. LLLT (660 nm, 30 mW and 5.4 J) was applied on the skin over the right upper bronchus and two irradiation protocols were used. Reduction of BHR post LLLT coincided with lower RhoA expression in Bronchial Muscle as well as reduction in eosinophils and eotaxin. LLLT also diminished ICAM expression and Th2 cytokines as well as signal transducer and activator of transduction 6 (STAT6) levels in lungs from challenged mice. Our results demonstrated that LLLT reduced BHR via RhoA and lessened allergic lung inflammation via STAT6.
