The Experts below are selected from a list of 193911 Experts worldwide ranked by ideXlab platform
Hongxin Fu - One of the best experts on this subject based on the ideXlab platform.
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salting out extraction and crystallization of succinic acid from fermentation Broths
Process Biochemistry, 2014Co-Authors: Hongxin FuAbstract:Abstract In this study, salting-out extraction (SOE) and crystallization were combined to recover succinic acid from fermentation Broths. Of the different SOE systems investigated, the system consisting of organic solvents and acidic salts appeared to be more favorable. A system using acetone and ammonium sulfate was investigated to determine the effect of phase composition and pH. The highest partition coefficient (8.64) and yield of succinic acid (90.05%) were obtained by a system composed of 30% (w/w) acetone and 20% (w/w) ammonium sulfate at a pH of 3.0. Additionally, 99.03% of cells, 90.82% of soluble proteins, and 94.89% of glucose could be simultaneously removed from the fermentation Broths. Interestingly, nearly 40% of the pigment was removed using the single-step salting-out extraction process. The analysis of the effect of pH on salting-out extraction indicates that a pH lower than the pK of succinic acid is beneficial for the recovery of succinic acid in an SOE system. Crystallization was performed for the purification of succinic acid at 4 °C and pH 2.0. By combining salting-out extraction with crystallization, an identical total yield (65%) and a higher purity (97%) of succinic acid were obtained using a synthetic fermentation broth compared with the actual fermentation broth (65% and 91%, respectively).
Hélène Roux-de Balmann - One of the best experts on this subject based on the ideXlab platform.
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A two steps membrane process for the recovery of succinic acid from fermentation broth
Separation and Purification Technology, 2018Co-Authors: Panwana Khunnonkwao, Kaemwich Jantama, Sunthorn Kanchanatawee, Sylvain Galier, Hélène Roux-de BalmannAbstract:The aim of this study was to investigate the integration of nanofiltration in succinic acid production based on a fermentation. An experimental investigation was carried out with NF 45 membrane and synthetic fermentation Broths of increasing complexity containing succinate salt and different impurities like inorganic salts, glucose or other organic acid salts like acetate. The influence of the operating conditions (pH, pressure…) as well as of the broth composition on the NF performances was studied. The mechanisms governing the transfer of the solutes through the membrane were investigated in order to explain the different solute retentions observed according to the fermentation broth composition. Finally, a two-step process including NF in a diafiltration mode followed by reverse osmosis was proposed to perform the purification of succinate from a synthetic fermentation broth containing acetate. It was shown that it is possible to increase the succinate purity from 85% to 99.5% while maintaining the total yield higher than 92%.
Jon Garciaaguirre - One of the best experts on this subject based on the ideXlab platform.
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up concentration of succinic acid lactic acid and ethanol fermentations Broths by forward osmosis
Biochemical Engineering Journal, 2020Co-Authors: Jon Garciaaguirre, Merlin Alvaradomorales, Ioannis A Fotidis, Irini AngelidakiAbstract:Abstract The potential of Forward Osmosis (FO) technology to up-concentrate succinic acid (HSuc), lactic acid (HLac), and ethanol (Eth) from fermentation Broths was investigated. The aforementioned molecules were obtained from two residual resources (biopulp and algal biomass) via anaerobic fermentation. HLac and Eth were produced from biopulp and HSuc was produced from macroalgal biomass hydrolysate. Herein, HLac, Eth, and HSuc titers of 14.98 ± 0.76, 19.11 ± 0.51 and 38.8 ± 0.32 g L−1 were obtained respectively. After treatment with centrifugation, FO was applied to the treated Broths using a thin film composite hollow fibre (TFHF) membrane. Best results were obtained with the HSuc fermentation broth using NaCl 5.0 M as draw solution. Final HSuc titer of 186.7 ± 9.3 g L−1 and water removal of 85% were attained. Findings in this work highlight a novel application of TFHF membranes.
Marius Turnea - One of the best experts on this subject based on the ideXlab platform.
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Comparative analysis of oxygen transfer rate distribution in stirred bioreactor for simulated and real fermentation Broths
Journal of Industrial Microbiology & Biotechnology, 2011Co-Authors: Dan Cascaval, Anca-irina Galaction, Marius TurneaAbstract:Study of the distribution of the oxygen mass transfer coefficient, k _l a , for a stirred bioreactor and simulated (pseudoplastic solutions of carboxymethylcellulose sodium salt) bacterial ( P. shermanii ), yeast ( S. cerevisiae ), and fungal ( P. chrysogenum free mycelia) Broths indicated significant variation of transfer rate with bioreactor height. The magnitude of the influence of the considered factors differed from one region to another. As a consequence of cell adsorption to bubble surface, the results indicated the impossibility of achieving a uniform oxygen transfer rate throughout the whole bulk of the microbial broth, even when respecting the conditions for uniform mixing. Owing to the different affinity of biomass for bubble surface, the positive influence of power input on k _l a is more important for fungal Broths, while increasing aeration is favorable only for simulated, bacterial and yeast Broths. The influence of the considered factors on k _l a were included in mathematical correlations established based on experimental data. For all considered positions, the proposed equations for real Broths have the general expression $$ k_{\rm l} a = \alpha C_{\rm X}^{\beta } \left( {{\frac{{P_{\rm a} }}{V}}} \right)^{\gamma } v_{\rm S}^{\delta } , $$ exhibiting good agreement with experimental results (with maximum deviations of ±10.7% for simulated Broths, ±8.4% for P. shermanii , ±9.3% for S. cerevisiae , and ±6.6% for P. chrysogenum ).
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Comparative analysis of mixing distribution in aerobic stirred bioreactor for simulated yeasts and fungus Broths
Journal of Industrial Microbiology & Biotechnology, 2007Co-Authors: Dan Cascaval, Anca-irina Galaction, Marius TurneaAbstract:The study on mixing distribution for an aerobic stirred bioreactor and simulated (solutions of carboxymethylcellulose sodium salt), yeasts ( S. cerevisiae ) and fungus ( P. chrysogenum pellets and free mycelia) Broths indicated the significant variation of mixing time on the bioreactor height. The experiments suggested the possibility to reach a uniform mixing in whole bulk of the real Broths for a certain value of rotation speed or biomass concentration domain. For S. cerevisiae Broths the optimum rotation speed increased to 500 rpm with the biomass accumulation from 40 to 150 g/l d.w. Irrespective of their morphology, for fungus cultures the existence of optimum rotation speed (500 rpm) has been recorded only for biomass concentration below 24 g/l d.w. The influence of aeration rate depends on the apparent viscosity/biomass concentration and on the impellers and sparger positions. By increasing the apparent viscosity for simulated Broths, or biomass amount for real Broths, the shape of the curves describing the mixing time variation is significantly changed for all the considered positions. The intensification of the aeration induced the increase of mixing time, which reached a maximum value, decreasing then, due to the flooding phenomena. This variation became more pronounced at higher viscosities for simulated Broths, at higher yeasts concentration, and at lower pellets or filamentous fungus concentration, respectively. By means of the experimental data and using MATLAB software, some mathematical correlations for mixing time have been proposed for each broth and considered position inside the bioreactor. These equations offer a good agreement with the experiment, the maximum deviation being ±7.3% for S. cerevisiae Broths.
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Enhancement of oxygen mass transfer in stirred bioreactors using oxygen-vectors 2. Propionibacterium shermanii Broths
Bioprocess and Biosystems Engineering, 2005Co-Authors: Anca-irina Galaction, Dan Cascaval, Marius Turnea, Elena FolescuAbstract:The previous works on simulated Broths are continued and developed for Propionibacterium shermanii Broths. The obtained results indicated the considerable increase of k _L a in presence of n -dodecane as oxygen-vector and the existence of a certain value of hydrocarbon concentration that corresponds to the maximum mass transfer rate of oxygen. The magnitude of the positive effect of the oxygen-vector strongly depends on operational conditions of the bioreactor, on broth characteristics and on P. shermanii concentration.
Panwana Khunnonkwao - One of the best experts on this subject based on the ideXlab platform.
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A two steps membrane process for the recovery of succinic acid from fermentation broth
Separation and Purification Technology, 2018Co-Authors: Panwana Khunnonkwao, Kaemwich Jantama, Sunthorn Kanchanatawee, Sylvain Galier, Hélène Roux-de BalmannAbstract:The aim of this study was to investigate the integration of nanofiltration in succinic acid production based on a fermentation. An experimental investigation was carried out with NF 45 membrane and synthetic fermentation Broths of increasing complexity containing succinate salt and different impurities like inorganic salts, glucose or other organic acid salts like acetate. The influence of the operating conditions (pH, pressure…) as well as of the broth composition on the NF performances was studied. The mechanisms governing the transfer of the solutes through the membrane were investigated in order to explain the different solute retentions observed according to the fermentation broth composition. Finally, a two-step process including NF in a diafiltration mode followed by reverse osmosis was proposed to perform the purification of succinate from a synthetic fermentation broth containing acetate. It was shown that it is possible to increase the succinate purity from 85% to 99.5% while maintaining the total yield higher than 92%.
