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Satish Kumar – 1st expert on this subject based on the ideXlab platform

  • a set of cattle microsatellite dna markers for genome analysis of riverine buffalo Bubalus bubalis
    Animal Genetics, 2002
    Co-Authors: Naveen Kumar Navani, P K Jain, Shraddha K Gupta, Brijesh S Sisodia, Satish Kumar


    Summary One hundred and eight microsatellite primer pairs, originally identified from cattle,were evaluated for their applicability in buffalo. Eighty-one primer pairs (75%)amplified discrete products, and of these, 61 pairs (56%) gave polymorphic bandpatterns on a panel of 25 buffaloes. The mean number of alleles per polymorphicmarker was 4.50 ‰ 0.20, and the mean heterozygosity per polymorphic marker was0.66 ‰ 0.02. Successful genotyping of buffaloes using cattle specific primers suggeststhat the latter can be a valuable resource for genome analysis in bubaline species.Keywords Bubalus bubalis, cattle, microsatellite, polymorphism, riverine buffalo.Deoxyribonucleic acid (DNA) polymorphisms are powerfultools for molecular dissection of traits of economic import-ance and for their potential applications in breeding moreproductive and efficient livestock. Deoxyribonucleic acidmarkers are also being used to understand the evolutionand domestication of these species. At present, microsatel-lites are the markers of choice for genome mapping, geneticdissection of complex traits and genetic diversity studiesbecause of their highly polymorphic nature, co-dominantmode of inheritance and ease of typing (e.g. Barendse et al.1994; Buchanan et al. 1994; Crawford et al. 1995; Hudsonet al. 1995; Rohrer et al. 1996; Takezaki & Nei 1996;Kappes et al. 1997). However, no genome mapping effortshave been devoted to riverine buffalo (Bubalus bubalis), themain dairy animal of the Indian subcontinent, andno systematic studies have been undertaken to developpolymorphic DNA markers in this species.Comparative genomic studies have shown that micro-satellite primer pair sequences are often conserved acrossrelated species and can be used for the development ofmarkers in related species. More than a thousand micro-satellite markers have been reported for cattle (Kappes et al.1997; BOVMAP). To develop a microsatellite based linkagemap of riverine buffalo, we have been evaluating cattlemicrosatellite primer pairs, and here we report the identifi-cation of 61 polymorphic markers suitable for genomeanalysis of riverine buffaloes.Genomic DNA was isolated from blood of 25 unrelatedanimals (Sambrook et al. 1989) representing three distinctand geographically defined buffalo breeds; viz. Murrah,Nili-Ravi and Mehsana. One cattle DNA sample was includedas positive control. Polymerase chain reaction (PCR) wascarried out on 100 ng of genomic DNA in a 50-ll reactionvolume. The reaction mixture consisted of 200 l

J P Dubey – 2nd expert on this subject based on the ideXlab platform

  • a review of coccidiosis in water buffaloes Bubalus bubalis
    Veterinary Parasitology, 2018
    Co-Authors: J P Dubey


    Abstract The water buffalo (Bubalus bubalis) is important to the economy of several countries in Asia, and South America and there are also isolated herds in Europe. In India, buffalo is the main dairy animal. Coccidiosis due to Eimeria is an important cause of diarrhea in livestock worldwide. Eimeria species are generally considered host specific. More than 11 Eimeria species are considered common in buffaloes and cattle (Bos taurus, Bos indicus). Of the numerous species of Eimeria in cattle, Eimeria bovis, Eimeria zuernii, Eimeria auburnensis and Eimeria alabamensis are considered pathogenic in cattle, but there is no report of their pathogenicity in naturally infected water buffaloes. Instead, Eimeria bareillyi has been documented to be the main pathogenic species in buffalo; it is not transmissible to cattle. There are many reports of Eimeria infections in water buffaloes, scattered in many local journals, and there are conflicting life cycle data on Eimeria species in buffalo. The objective of this paper is to summarize information on history, validity of Eimeria species, life cycle, pathogenicity, prevalence, epidemiology, diagnosis and control of coccidiosis in buffalo.

Giuseppe Cringoli – 3rd expert on this subject based on the ideXlab platform

  • Cystic echinococcosis in water buffaloes (Bubalus bubalis)
    Italian Journal of Animal Science, 2016
    Co-Authors: Federico Capuano, Laura Rinaldi, M.p. Maurelli, A. G. Perugini, V. Veneziano, V. Musella, Giuseppe Cringoli


    An epidemiological and molecular survey of cystic echinococcosis (CE) caused by Echinococcus granulosus in the water buffalo (Bubalus bubalis) of the Italian Mediterranean breed was carried out in the Campania region of southern Italy. Out of a total of 799 water buffaloes examined at slaughterhouses, 80 (10.0%) were found infected. The molecular study was performed on 58 hydatid cysts in order to determine the E. granulosus strain(s) present in this host. A region of cytocrome c oxydase 1 gene (CO1) was amplified by polymerase chain reaction and the PCR products were then purified and sequenced. DNA amplification of the partial CO1 gene gave a 446 bp fragment for all isolates examined. After sequencing, a region of 419 bp was identified for each sample. Thirty-two isolates were identified as the common sheep strain G1, 15 as the buffalo strain G3, 3 as the Tasmanian sheep strain G2, and 3 as the G1 c genotype (GenBank AF458873). In addition, 5 isolates presented 99% identity with the G2 genotype (Tasmanian sheep strain).

  • Cryptosporidium and Giardia in water buffaloes (Bubalus bubalis) of the Italian Mediterranean bred.
    Veterinary Research Communications, 2007
    Co-Authors: Laura Rinaldi, R. U. Condoleo, G. Saralli, G. Bruni, Giuseppe Cringoli


    Rinaldi, L., Condoleo, R.U., Condoleo, R., Saralli, G., Bruni, G. and Cringoli, G., 2007. Cryptosporidium and Giardia in water buffaloes (Bubalus bubalis) of the Italian Mediterranean bred. Veterinary Research Communications, 31(Suppl. 1), 253–255

  • neosporosis in water buffalo Bubalus bubalis in southern italy
    Veterinary Parasitology, 2000
    Co-Authors: A Guarino, Giovanna Fusco, Giovanni Savini, G Di Francesco, Giuseppe Cringoli


    A study was carried on 1377 water buffalo serum samples from 50 farms in southern Italy to test the presence of Neospora caninum antibodies by indirect fluorescence antibody test (IFAT). Rabbit anti-buffalo immunoglobulins conjugated to fluorescein were used in the test. Fluorescence in sera dilutions above 1:200 was considered as indicative of the presence of N. caninum antibodies. The overall prevalence of infection in the animals was 34.6%. The prevalence increased in relation to the age of subjects and most of the herds examined (82%) were found infected. In two farms abortions and neurological signs were reported. No suppurative inflammatory lesions were seen, but few protozoan-like cysts were observed on foetal tissues by histology.