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Satish Kumar - One of the best experts on this subject based on the ideXlab platform.
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a set of cattle microsatellite dna markers for genome analysis of riverine buffalo Bubalus bubalis
Animal Genetics, 2002Co-Authors: Naveen Kumar Navani, P K Jain, Shraddha K Gupta, Brijesh S Sisodia, Satish KumarAbstract:Summary One hundred and eight microsatellite primer pairs, originally identified from cattle,were evaluated for their applicability in buffalo. Eighty-one primer pairs (75%)amplified discrete products, and of these, 61 pairs (56%) gave polymorphic bandpatterns on a panel of 25 buffaloes. The mean number of alleles per polymorphicmarker was 4.50 ‰ 0.20, and the mean heterozygosity per polymorphic marker was0.66 ‰ 0.02. Successful genotyping of buffaloes using cattle specific primers suggeststhat the latter can be a valuable resource for genome analysis in bubaline species.Keywords Bubalus bubalis, cattle, microsatellite, polymorphism, riverine buffalo.Deoxyribonucleic acid (DNA) polymorphisms are powerfultools for molecular dissection of traits of economic import-ance and for their potential applications in breeding moreproductive and efficient livestock. Deoxyribonucleic acidmarkers are also being used to understand the evolutionand domestication of these species. At present, microsatel-lites are the markers of choice for genome mapping, geneticdissection of complex traits and genetic diversity studiesbecause of their highly polymorphic nature, co-dominantmode of inheritance and ease of typing (e.g. Barendse et al.1994; Buchanan et al. 1994; Crawford et al. 1995; Hudsonet al. 1995; Rohrer et al. 1996; Takezaki & Nei 1996;Kappes et al. 1997). However, no genome mapping effortshave been devoted to riverine buffalo (Bubalus bubalis), themain dairy animal of the Indian subcontinent, andno systematic studies have been undertaken to developpolymorphic DNA markers in this species.Comparative genomic studies have shown that micro-satellite primer pair sequences are often conserved acrossrelated species and can be used for the development ofmarkers in related species. More than a thousand micro-satellite markers have been reported for cattle (Kappes et al.1997; BOVMAP). To develop a microsatellite based linkagemap of riverine buffalo, we have been evaluating cattlemicrosatellite primer pairs, and here we report the identifi-cation of 61 polymorphic markers suitable for genomeanalysis of riverine buffaloes.Genomic DNA was isolated from blood of 25 unrelatedanimals (Sambrook et al. 1989) representing three distinctand geographically defined buffalo breeds; viz. Murrah,Nili-Ravi and Mehsana. One cattle DNA sample was includedas positive control. Polymerase chain reaction (PCR) wascarried out on 100 ng of genomic DNA in a 50-ll reactionvolume. The reaction mixture consisted of 200 l
J P Dubey - One of the best experts on this subject based on the ideXlab platform.
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a review of coccidiosis in water buffaloes Bubalus bubalis
Veterinary Parasitology, 2018Co-Authors: J P DubeyAbstract:Abstract The water buffalo (Bubalus bubalis) is important to the economy of several countries in Asia, and South America and there are also isolated herds in Europe. In India, buffalo is the main dairy animal. Coccidiosis due to Eimeria is an important cause of diarrhea in livestock worldwide. Eimeria species are generally considered host specific. More than 11 Eimeria species are considered common in buffaloes and cattle (Bos taurus, Bos indicus). Of the numerous species of Eimeria in cattle, Eimeria bovis, Eimeria zuernii, Eimeria auburnensis and Eimeria alabamensis are considered pathogenic in cattle, but there is no report of their pathogenicity in naturally infected water buffaloes. Instead, Eimeria bareillyi has been documented to be the main pathogenic species in buffalo; it is not transmissible to cattle. There are many reports of Eimeria infections in water buffaloes, scattered in many local journals, and there are conflicting life cycle data on Eimeria species in buffalo. The objective of this paper is to summarize information on history, validity of Eimeria species, life cycle, pathogenicity, prevalence, epidemiology, diagnosis and control of coccidiosis in buffalo.
Giuseppe Cringoli - One of the best experts on this subject based on the ideXlab platform.
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Cystic echinococcosis in water buffaloes (Bubalus bubalis)
Italian Journal of Animal Science, 2016Co-Authors: Federico Capuano, Laura Rinaldi, M.p. Maurelli, A. G. Perugini, V. Veneziano, V. Musella, Giuseppe CringoliAbstract:An epidemiological and molecular survey of cystic echinococcosis (CE) caused by Echinococcus granulosus in the water buffalo (Bubalus bubalis) of the Italian Mediterranean breed was carried out in the Campania region of southern Italy. Out of a total of 799 water buffaloes examined at slaughterhouses, 80 (10.0%) were found infected. The molecular study was performed on 58 hydatid cysts in order to determine the E. granulosus strain(s) present in this host. A region of cytocrome c oxydase 1 gene (CO1) was amplified by polymerase chain reaction and the PCR products were then purified and sequenced. DNA amplification of the partial CO1 gene gave a 446 bp fragment for all isolates examined. After sequencing, a region of 419 bp was identified for each sample. Thirty-two isolates were identified as the common sheep strain G1, 15 as the buffalo strain G3, 3 as the Tasmanian sheep strain G2, and 3 as the G1 c genotype (GenBank AF458873). In addition, 5 isolates presented 99% identity with the G2 genotype (Tasmanian sheep strain).
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Cryptosporidium and Giardia in water buffaloes (Bubalus bubalis) of the Italian Mediterranean bred.
Veterinary Research Communications, 2007Co-Authors: Laura Rinaldi, R. U. Condoleo, G. Saralli, G. Bruni, Giuseppe CringoliAbstract:Rinaldi, L., Condoleo, R.U., Condoleo, R., Saralli, G., Bruni, G. and Cringoli, G., 2007. Cryptosporidium and Giardia in water buffaloes (Bubalus bubalis) of the Italian Mediterranean bred. Veterinary Research Communications, 31(Suppl. 1), 253–255
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neosporosis in water buffalo Bubalus bubalis in southern italy
Veterinary Parasitology, 2000Co-Authors: A Guarino, Giovanna Fusco, Giovanni Savini, G Di Francesco, Giuseppe CringoliAbstract:A study was carried on 1377 water buffalo serum samples from 50 farms in southern Italy to test the presence of Neospora caninum antibodies by indirect fluorescence antibody test (IFAT). Rabbit anti-buffalo immunoglobulins conjugated to fluorescein were used in the test. Fluorescence in sera dilutions above 1:200 was considered as indicative of the presence of N. caninum antibodies. The overall prevalence of infection in the animals was 34.6%. The prevalence increased in relation to the age of subjects and most of the herds examined (82%) were found infected. In two farms abortions and neurological signs were reported. No suppurative inflammatory lesions were seen, but few protozoan-like cysts were observed on foetal tissues by histology.
V Sharma - One of the best experts on this subject based on the ideXlab platform.
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folliculogenesis in buffalo Bubalus bubalis a review
Reproduction Fertility and Development, 2002Co-Authors: R S Manik, P Palta, S K Singla, V SharmaAbstract:The urgent need for improving the reproductive performance of buffalo necessitates a better understanding of the mechanisms controlling ovarian follicular growth and development. Attention needs to be focused on improving superovulation responses and conception rates, and reducing the variability in ovulation rate and embryo loss. Application of ultrasonic imaging has revealed that follicular turnover during an unstimulated oestrous cycle occurs in waves, with each wave involving synchronous development of a group of follicles, one dominant and several subordinate follicles. There is a predominance of two waves with the first wave beginning around Day 0 (day of ovulation) and the second wave around Day 9 or 10. Primary reasons for a lower superovulation response in buffalo compared with that in cattle is a lower number of primordial and antral follicles, a slower shift from small to large follicles during superovulation, a higher incidence of deep atresia and inability of several large follicles to ovulate, especially when superovulation is induced by equine chorionic gonadotrophin treatment. There is near complete lack of information in Bubalus bubalis on the factors controlling the selection of the dominant follicle, the period of functional dominance and the effects of environmental factors, such as climate and nutrition, on follicular dynamics.
Carlos Wilson Gomes Lopes - One of the best experts on this subject based on the ideXlab platform.
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Eimeria bareillyi FROM THE DOMESTIC WATER BUFFALO, Bubalus bubalis, IN THE STATE OF RIO DE JANEIRO, BRAZIL* Eimeria bareillyi DO BÚFALO DOMÉSTICO, Bubalus bubalis, NO ESTADO DO RIO DE JANEIRO, BRASIL
2020Co-Authors: Landreani Ramirez, Bruno Pereira Berto, Walter Leira, Teixeira Filho, Gisele Santos De Meireles, Janaina Da Soledad Rodrigues, Claudio Rogério Rocha Almeida, Carlos Wilson Gomes LopesAbstract:Ramirez, L.; Berto, B.P.; Teixeira Filho, W.L.; Flausino, W.; Meireles, G. S. de; Rodrigues, J. da S.; Almeida, C.R.R. & Lopes, C.W.G. Eimeria bareillyi from the domestic water buffalo, Bubalus bubalis, in the State of Rio de Janeiro, Brazil. (Eimeria bareillyi do bufalo domestico, Bubalus bubalis, no estado do Rio de Janeiro, Brasil.) Revista Brasileira de Medicina Veterinaria, 31(4):261-264, 2009. Curso de Pos-Graduacao em Ciencias Veterinarias, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 07, Seropedica, RJ 23890-000, Brasil. E-mail: landrynana@hotmail.com This study reports domestic water buffalos, Bubalus bubalis, parasitized by Eimeria bareillyi. Its oocyts were pyriform, with smooth, bilayered wall, 29.5 (27-33) x 21.3 (20-24) μm length and width respectively. Micropyle was present, without a micropyle cap. Polar granule and oocyst residuum were absent. Sporocysts were elongating ellipsoidal. Stieda body was present; however, substieda and parastieda bodies were absent. Sporocyst residuum was present and sporozoites presented a refractile body and a nucleus. Sporulated oocysts of E. bareillyi were uniforms in their distribution, evidencing the presence of a single species in spite of polymorphic in its shape.
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Coccidiose natural em búfalos (Bubalus bubalis L.1875) no Sudeste do Brasil
2016Co-Authors: Walter Leira Teixeira Filho, Landreani Ramirez Gonçalves, Carlos Wilson Gomes LopesAbstract:ABSTRACT. Teixeira Filho W.L., Goncaves L.R. & Lopes C.W.G. Natural coccidiosis infection in water buffaloes (Bubalus bubalis L. 1875) in Southeastern Brazil. [Coccidiose natural em bufalos (Bubalus bubalis L. 1875) no Sudeste do Brasil.] Frequencia das especies do genero Eimeria Schneider, 1885 (Apicomplexa: Eimeriidae) em Bubalus bubalis Linnaeus, 1875 no sudeste do Brasil. Revista Brasileira de Medicina Veterinaria, 38 (supl. 3):1-8, 2016. Departamento de Parasitologia Animal, Anexo 1, Instituto de Veterinaria, Universidade Federal Rural do Rio de Janeiro. Campus Seropedica, BR 465 km 7, RJ 23890-000, Brasil.E-mail: wleira@bol.com.br Herd of buffaloes in Brazil is related to extensive breedings, where the oc-cupation of the land is premised on its permanence for being a rustic animal. According to the analyzed data obtained from oocysts of the genus Eimeria recovered from fecal samples and placed to sporulate in 2.5% potassium dichromate. Regarding the identification of the species, the description and measurements of the sporulated oocysts were taken for each identified species. In relation to the morphotypes found, the presence of three specific species from water buffalo, such as: Eimeria bareylli, Eimeria ankarensis and Eimeria gokaki were observed, where the last two species were first reported in Brazil. The most prevalent species in water buffalos in the State of Rio de Janeiro was Eimeria zuernii followed by Eimeria ellipsoidalis both and other of less frequent species were also very common in bovine. Species distribution of the genus Eimeria was not homogeneous in all analized animals, where an animal has eight species, while 88.75% of the total samples had at least four species. The buffalo herds were characterized as extensive breeding, where the animals were keptin place for a long time, regardless of age, and sex and from youth to adults.
