The Experts below are selected from a list of 21153 Experts worldwide ranked by ideXlab platform
Arto Urtti - One of the best experts on this subject based on the ideXlab platform.
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accelerated pharmaceutical protein development with integrated cell free expression purification and bioconjugation
Scientific Reports, 2018Co-Authors: Dominique Richardson, Jaakko Itkonen, Julia Nievas, Arto Urtti, Marco G. CasteleijnAbstract:The use of living cells for the synthesis of pharmaceutical proteins, though state-of-the-art, is hindered by its lengthy process comprising of many steps that may affect the protein’s stability and activity. We aimed to integrate protein expression, purification, and bioconjugation in small Volumes coupled with cell free protein synthesis for the target protein, ciliary neurotrophic factor. Split-intein mediated capture by use of capture peptides onto a solid surface was efficient at 89–93%. Proof-of-principle of light triggered release was compared to affinity chromatography (His6 fusion tag coupled with Ni-NTA). The latter was more efficient, but more time consuming. Light triggered release was clearly demonstrated. Moreover, we transferred biotin from the capture peptide to the target protein without further purification steps. Finally, the target protein was released in a Buffer-Volume and composition of our choice, omitting the need for protein concentration or changing the Buffer. Split-intein mediated capture, protein trans splicing followed by light triggered release, and bioconjugation for proteins synthesized in cell free systems might be performed in an integrated workflow resulting in the fast production of the target protein.
Longwei Yin - One of the best experts on this subject based on the ideXlab platform.
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metal organic frameworks derived porous core shellcop c polyhedrons anchored on 3d reduced graphene oxide networks as anode for sodium ion battery
Nano Energy, 2017Co-Authors: Longwei YinAbstract:Abstract A novel strategy is developed to synthesize metal-organic framework (MOF) derived core/shell structured CoP@C polyhedrons anchored on 3D reduced grapheme oxide (RGO) on nickel foam (NF) as binder-free anode for high performance sodium-ion battery, through an in-situ low-temperature phosphidation process from ZIF-67 derived core/shell Co@C polyhedral structures. The unique CoP@C-RGO-NF binder-free anode exhibits a remarkable electrochemical performance with outstanding cycling stability and high rate capability, delivering a specific capacity of 473.1 mA h g −1 at a current density of 100 mA g −1 after 100 cycles. The excellent properties can be attributed to synergistic effects between core/shell CoP@C polyhedrons and RGO networks. The unique core/shell CoP@C polyhedrons can offer more electrode/electrolyte contact area and reduce the diffusion distance of Na + , while carbon layer shell can enhance electronic conductivity and Buffer Volume change, and prevent CoP from pulverization and aggregation. Furthermore, 3D RGO networks can provide adequate surface areas for a high loading content of CoP and enhance charge transfer kinetics. Meanwhile, RGO/NF can efficiently act as a binder and electrical conductor to interconnect the separate CoP@C polyhedrons. The present strategy for CoP@C-RGO-NF architectures can be extended to other novel electrodes for high performance energy storage devices.
Margareta Hammarlundudenaes - One of the best experts on this subject based on the ideXlab platform.
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development of a high throughput brain slice method for studying drug distribution in the central nervous system
Drug Metabolism and Disposition, 2009Co-Authors: Markus Fridén, Frederic Ducrozet, Brian Middleton, Madeleine Antonsson, Ulf Bredberg, Margareta HammarlundudenaesAbstract:New, more efficient methods of estimating unbound drug concentrations in the central nervous system (CNS) combine the amount of drug in whole brain tissue samples measured by conventional methods with in vitro estimates of the unbound brain Volume of distribution ( V u,brain ). Although the brain slice method is the most reliable in vitro method for measuring V u,brain , it has not previously been adapted for the needs of drug discovery research. The aim of this study was to increase the throughput and optimize the experimental conditions of this method. Equilibrium of drug between the Buffer and the brain slice within the 4 to 5 h of incubation is a fundamental requirement. However, it is difficult to meet this requirement for many of the extensively binding, lipophilic compounds in drug discovery programs. In this study, the dimensions of the incubation vessel and mode of stirring influenced the equilibration time, as did the amount of brain tissue per unit of Buffer Volume. The use of casette experiments for investigating V u,brain in a linear drug concentration range increased the throughput of the method. The V u,brain for the model compounds ranged from 4 to 3000 ml · g brain –1 , and the sources of variability are discussed. The optimized setup of the brain slice method allows precise, robust estimation of V u,brain for drugs with diverse properties, including highly lipophilic compounds. This is a critical step forward for the implementation of relevant measurements of CNS exposure in the drug discovery setting.
Marco G. Casteleijn - One of the best experts on this subject based on the ideXlab platform.
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accelerated pharmaceutical protein development with integrated cell free expression purification and bioconjugation
Scientific Reports, 2018Co-Authors: Dominique Richardson, Jaakko Itkonen, Julia Nievas, Arto Urtti, Marco G. CasteleijnAbstract:The use of living cells for the synthesis of pharmaceutical proteins, though state-of-the-art, is hindered by its lengthy process comprising of many steps that may affect the protein’s stability and activity. We aimed to integrate protein expression, purification, and bioconjugation in small Volumes coupled with cell free protein synthesis for the target protein, ciliary neurotrophic factor. Split-intein mediated capture by use of capture peptides onto a solid surface was efficient at 89–93%. Proof-of-principle of light triggered release was compared to affinity chromatography (His6 fusion tag coupled with Ni-NTA). The latter was more efficient, but more time consuming. Light triggered release was clearly demonstrated. Moreover, we transferred biotin from the capture peptide to the target protein without further purification steps. Finally, the target protein was released in a Buffer-Volume and composition of our choice, omitting the need for protein concentration or changing the Buffer. Split-intein mediated capture, protein trans splicing followed by light triggered release, and bioconjugation for proteins synthesized in cell free systems might be performed in an integrated workflow resulting in the fast production of the target protein.
Guoquan Suo - One of the best experts on this subject based on the ideXlab platform.
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flexible n doped carbon bubble like mos2 core sheath framework Buffering Volume expansion for potassium ion batteries
Journal of Colloid and Interface Science, 2020Co-Authors: Guoquan Suo, Jiaqi Zhang, Lei Feng, Xiaojiang Hou, Yanling Yang, Li Zhang, Wei Alex WangAbstract:Abstract Suitable anode materials for potassium ion batteries (KIBs) with high capacity, good reversibility and stable cycling performances are still in large demand. Here, flexible N doped carbon/bubble-like MoS2 core/sheath framework (MoS2/NCS) is prepared as an anode material for potassium ion batteries. The N doped carbon sponge (NCS) skeleton with good conductivity and high surface area guarantees superior rate capability and high stability of MoS2/NCS anode. The chemical bonds (C Mo) firmly bridge MoS2 and NCS together, which further ensures MoS2/NCS stable cycling performance. More importantly, Volume expansion is greatly Buffered during cycling by this unique structure: the voids between bubble-like MoS2 sheath and NCS core can effectively Buffer Volume expansion generated during potassium intercalation/deintercalation; the enlarged interlayer spacing contribute more space to Buffer Volume change; the ultrathin nanosheets can shorten the charge diffusion distance and Buffer Volume change. As a consequence, MoS2/NCS delivers a capacity of 374 mAh g−1 over 200 cycles at 50 mA g−1. Even at 1000 mA g−1, a capacity of 212 mAh g−1 can still be obtained over 1000 cycles. We believe this MoS2/NCS structure will highlight the potential of MoS2 in practical KIBs applications.
