The Experts below are selected from a list of 1839 Experts worldwide ranked by ideXlab platform
Jo Ann M. Burkholder - One of the best experts on this subject based on the ideXlab platform.
-
Use of Molecular Probes to Assess Geographic Distribution of Pfiesteria Species
2013Co-Authors: Parke A. Rublee, Jo Ann M. Burkholder, David W. Oldach, Torstein Tengs, Jason W. Kempton, Eric F. Schaefer, Coy Allen, Janera Harris, Holly Bowers, H. B. GlasgowAbstract:We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts were assayed for the presence of Pfiesteria piscicida Steidinger & Burkholder and Pfiesteria shumwayae Glasgow & Burkholder by PCR probing of extracted DNA. Positive results were found in about 3 % of samples derived from routine monitoring of coastal waters and about 8 % of sediments. The geographic range of both species was the same, ranging from New York to Texas. Pfiesteria spp. are likely common and generally benign inhabitants of coastal areas, but their presence maintains a potential for fish and human health problems. Key words: molecular probes, PCR, Pfiesteria, toxic dinoflagellates. — Enviro
-
Taxonomy of Pfiesteria (Dinophyceae)
Harmful Algae, 2006Co-Authors: Harold G. Marshall, Matthew W. Parrow, Elle H. Allen, Valerie M. Knowlton, Parke A. Rublee, Malte Elbrachter, Paul E. Hargraves, Jo Ann M. Burkholder, Wayne L. Hynes, Todd A. EgertonAbstract:The dinoflagellate species originally described as Pfiesteria shumwayae Glasgow et Burkholder, recently transferred to a new genus, Pseudopfiesteria Litaker et al., is reclassified into the redefined genus Pfiesteria Steidinger et Burkholder, as Pfiesteria shumwayae within the order Peridiniales. This change is based upon consideration of a compilation of previous and new morphological analyses and molecular phylogenetic analyses. Morphological analysis with scanning and transmission electron microscopy supports previous findings except in the sulcal area. In the cells examined, the sulcus is partly concealed by the peduncle cover plate (p.c.), which originates at the right side of the sulcus along the left side of the 6c and 5? plates. The fine structure of the p.c. appears similar to that of other thecal plates. The 1? plate can also extend slightly over the sulcus. Transmission electron microscopy revealed that Pfiesteria shumwayae can have at least six sulcal plates; the number remains uncertain and may vary. The sulcal plates of this small, delicately thecate species have not been clearly discerned by scanning electron microscopy of membrane-stripped and/or suture-swollen cells. The Kofoidian thecal plate formula for the genus Pfiesteria is Po, cp, X, 4', la, 5–6?, 6c, p.c., ?s, 5?, 0p, 2?'. The monophyletic grouping of ?pfiesteria-like? taxa within the order Peridiniales, as well as the grouping of Pfiesteria piscicida and Pfiesteria shumwayae within the same genus, is also supported by the preponderance of previous molecular evidence, and by the phylogenetic trees contributed in the present analysis. Pfiesteria appears to be closely related to as-yet informally described cryptoperidiniopsoids and calcareous dinoflagellates such as Thoracosphaera; thus, the family classification requires revision that is beyond the scope of this study.
-
Re-evaluation of the Relationship between Pfiesteria and Estuarine Fish Kills
Ecosystems, 2003Co-Authors: Cavell Brownie, Robert E. Reed, Jo Ann M. Burkholder, Yongqiang TangAbstract:In recent years, fish kills along the mid-Atlantic US coast have become an increasing problem, with important economic, environmental, and public health implications (Glasgow and others 1995; Burkholder 1998; Grattan and others 1998; Haselow and others 2001; Shoemaker and Hudnell 2001). Research into the causes of these fish kills is ongoing, and monitoring and surveillance programs have been instituted to investigate (among other factors) the role of actively toxic forms of two known species within the dinoflagellate genus Pfiesteria (Burkholder and others 1995, 2001a; Steidinger and others 1996; Burkholder and Glasgow 1997; Glasgow and others 2001b). In their recent analyses of the relationship between Pfiesteria and fish kills, Burkholder and others (1999) and Stow (1999) stated, as others have noted previously (Meyer and Barclay 1990), that it is difficult to establish the causes of estuarine fish kills at the ecosystem level. The evaluation of Burkholder and others (1999) was based on the biology and toxic behavior of Pfiesteria, as well as empirical sampling of field fish kill events then in progress, as supported by laboratory analyses of samples collected from each fish kill. In contrast, Stow (1999) conducted theoretical probability calculations and argued that information demonstrating the presence of toxic Pfiesteria during fish kills was insufficient to prove that there was a cause-and-effect
-
Use of molecular probes to assess geographic distribution of Pfiesteria species
Environmental Health Perspectives, 2001Co-Authors: Parke A. Rublee, Jo Ann M. Burkholder, David W. Oldach, Torstein Tengs, Jason W. Kempton, Eric F. Schaefer, Holly A. Bowers, Coy Allen, Janera Harris, H. B. GlasgowAbstract:We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts were assayed for the presence of Pfiesteria piscicida Steidinger & Burkholder and Pfiesteria shumwayae Glasgow & Burkholder by PCR probing of extracted DNA. Positive results were found in about 3% of samples derived from routine monitoring of coastal waters and about 8% of sediments. The geographic range of both species was the same, ranging from New York to Texas. Pfiesteria spp. are likely common and generally benign inhabitants of coastal areas, but their presence maintains a potential for fish and human health problems.
Parke A. Rublee - One of the best experts on this subject based on the ideXlab platform.
-
Use of Molecular Probes to Assess Geographic Distribution of Pfiesteria Species
2013Co-Authors: Parke A. Rublee, Jo Ann M. Burkholder, David W. Oldach, Torstein Tengs, Jason W. Kempton, Eric F. Schaefer, Coy Allen, Janera Harris, Holly Bowers, H. B. GlasgowAbstract:We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts were assayed for the presence of Pfiesteria piscicida Steidinger & Burkholder and Pfiesteria shumwayae Glasgow & Burkholder by PCR probing of extracted DNA. Positive results were found in about 3 % of samples derived from routine monitoring of coastal waters and about 8 % of sediments. The geographic range of both species was the same, ranging from New York to Texas. Pfiesteria spp. are likely common and generally benign inhabitants of coastal areas, but their presence maintains a potential for fish and human health problems. Key words: molecular probes, PCR, Pfiesteria, toxic dinoflagellates. — Enviro
-
Taxonomy of Pfiesteria (Dinophyceae)
Harmful Algae, 2006Co-Authors: Harold G. Marshall, Matthew W. Parrow, Elle H. Allen, Valerie M. Knowlton, Parke A. Rublee, Malte Elbrachter, Paul E. Hargraves, Jo Ann M. Burkholder, Wayne L. Hynes, Todd A. EgertonAbstract:The dinoflagellate species originally described as Pfiesteria shumwayae Glasgow et Burkholder, recently transferred to a new genus, Pseudopfiesteria Litaker et al., is reclassified into the redefined genus Pfiesteria Steidinger et Burkholder, as Pfiesteria shumwayae within the order Peridiniales. This change is based upon consideration of a compilation of previous and new morphological analyses and molecular phylogenetic analyses. Morphological analysis with scanning and transmission electron microscopy supports previous findings except in the sulcal area. In the cells examined, the sulcus is partly concealed by the peduncle cover plate (p.c.), which originates at the right side of the sulcus along the left side of the 6c and 5? plates. The fine structure of the p.c. appears similar to that of other thecal plates. The 1? plate can also extend slightly over the sulcus. Transmission electron microscopy revealed that Pfiesteria shumwayae can have at least six sulcal plates; the number remains uncertain and may vary. The sulcal plates of this small, delicately thecate species have not been clearly discerned by scanning electron microscopy of membrane-stripped and/or suture-swollen cells. The Kofoidian thecal plate formula for the genus Pfiesteria is Po, cp, X, 4', la, 5–6?, 6c, p.c., ?s, 5?, 0p, 2?'. The monophyletic grouping of ?pfiesteria-like? taxa within the order Peridiniales, as well as the grouping of Pfiesteria piscicida and Pfiesteria shumwayae within the same genus, is also supported by the preponderance of previous molecular evidence, and by the phylogenetic trees contributed in the present analysis. Pfiesteria appears to be closely related to as-yet informally described cryptoperidiniopsoids and calcareous dinoflagellates such as Thoracosphaera; thus, the family classification requires revision that is beyond the scope of this study.
-
Use of molecular probes to assess geographic distribution of Pfiesteria species
Environmental Health Perspectives, 2001Co-Authors: Parke A. Rublee, Jo Ann M. Burkholder, David W. Oldach, Torstein Tengs, Jason W. Kempton, Eric F. Schaefer, Holly A. Bowers, Coy Allen, Janera Harris, H. B. GlasgowAbstract:We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts were assayed for the presence of Pfiesteria piscicida Steidinger & Burkholder and Pfiesteria shumwayae Glasgow & Burkholder by PCR probing of extracted DNA. Positive results were found in about 3% of samples derived from routine monitoring of coastal waters and about 8% of sediments. The geographic range of both species was the same, ranging from New York to Texas. Pfiesteria spp. are likely common and generally benign inhabitants of coastal areas, but their presence maintains a potential for fish and human health problems.
H. B. Glasgow - One of the best experts on this subject based on the ideXlab platform.
-
Use of Molecular Probes to Assess Geographic Distribution of Pfiesteria Species
2013Co-Authors: Parke A. Rublee, Jo Ann M. Burkholder, David W. Oldach, Torstein Tengs, Jason W. Kempton, Eric F. Schaefer, Coy Allen, Janera Harris, Holly Bowers, H. B. GlasgowAbstract:We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts were assayed for the presence of Pfiesteria piscicida Steidinger & Burkholder and Pfiesteria shumwayae Glasgow & Burkholder by PCR probing of extracted DNA. Positive results were found in about 3 % of samples derived from routine monitoring of coastal waters and about 8 % of sediments. The geographic range of both species was the same, ranging from New York to Texas. Pfiesteria spp. are likely common and generally benign inhabitants of coastal areas, but their presence maintains a potential for fish and human health problems. Key words: molecular probes, PCR, Pfiesteria, toxic dinoflagellates. — Enviro
-
Use of molecular probes to assess geographic distribution of Pfiesteria species
Environmental Health Perspectives, 2001Co-Authors: Parke A. Rublee, Jo Ann M. Burkholder, David W. Oldach, Torstein Tengs, Jason W. Kempton, Eric F. Schaefer, Holly A. Bowers, Coy Allen, Janera Harris, H. B. GlasgowAbstract:We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts were assayed for the presence of Pfiesteria piscicida Steidinger & Burkholder and Pfiesteria shumwayae Glasgow & Burkholder by PCR probing of extracted DNA. Positive results were found in about 3% of samples derived from routine monitoring of coastal waters and about 8% of sediments. The geographic range of both species was the same, ranging from New York to Texas. Pfiesteria spp. are likely common and generally benign inhabitants of coastal areas, but their presence maintains a potential for fish and human health problems.
Deborah Koltai - One of the best experts on this subject based on the ideXlab platform.
-
Potential Human Health Effects Associated with Laboratory Exposures to Pfiesteria piscicida
2013Co-Authors: Deborah Koltai, Pfiesteria Piscicida SteidingerAbstract:The adverse human health effects associated with the most prolonged and intense exposure known to Pfiesteria piscicida Steidinger & Burkholder cultures and toxin(s) are described. In December 1993, a patient presented with acute illness to the Memory Disorders Clinic of the Brya
-
Potential human health effects associated with laboratory exposures to Pfiesteria piscicida.
Environmental Health Perspectives, 2001Co-Authors: Deborah KoltaiAbstract:The adverse human health effects associated with the most prolonged and intense exposure known to Pfiesteria piscicida Steidinger & Burkholder cultures and toxin(s) are described. In December 1993,...
Kai Liu - One of the best experts on this subject based on the ideXlab platform.
-
on regularity property of retarded ornstein uhlenbeck processes in hilbert spaces
Journal of Theoretical Probability, 2012Co-Authors: Kai LiuAbstract:In this work, some regularity properties of mild solutions for a class of stochastic linear functional differential equations driven by infinite-dimensional Wiener processes are considered. In terms of retarded fundamental solutions, we introduce a class of stochastic convolutions which naturally arise in the solutions and investigate their Yosida approximants. By means of the retarded fundamental solutions, we find conditions under which each mild solution permits a continuous modification. With the aid of Yosida approximation, we study two kinds of regularity properties, temporal and spatial ones, for the retarded solution processes. By employing a factorization method, we establish a retarded version of the Burkholder–Davis–Gundy inequality for stochastic convolutions.
-
on regularity property of retarded ornstein uhlenbeck processes in hilbert spaces
arXiv: Probability, 2011Co-Authors: Kai LiuAbstract:In this work, some regularity properties of mild solutions for a class of stochastic linear functional differential equations driven by infinite dimensional Wiener processes are considered. In terms of retarded fundamental solutions, we introduce a class of stochastic convolutions which naturally arise in the solutions and investigate their Yosida approximants. By means of the retarded fundamental solutions, we find conditions under which each mild solution permits a continuous modification. With the aid of Yosida approximation, we study two kinds of regularity properties, temporal and spatial ones, for the retarded solution processes. By employing a factorization method, we establish a retarded version of Burkholder-Davis-Gundy's inequality for stochastic convolutions.
