The Experts below are selected from a list of 522 Experts worldwide ranked by ideXlab platform
Kavi Ratanabanangkoon - One of the best experts on this subject based on the ideXlab platform.
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development of an in vitro potency assay for antivenom against malayan pit viper Calloselasma rhodostoma
Toxicon, 2014Co-Authors: Duangporn Pornmuttakun, Kavi RatanabanangkoonAbstract:Abstract An in vitro potency assay of antivenom against Malayan pit viper (Calloselasma rhodostoma, CR) has been developed. The assay is based on the neutralizing activity of the antivenom against the coagulant activity of the venom. The minimum coagulant dose (MCD) of CR venom was 22.12 ± 0.25 μg/ml. The coagulation time induced by 2MCD of the venom was used as the control for calculating the neutralizing activity of each batch of antivenom. The in vitro potency of antivenom, expressed as effective dose (ED), was the antivenom/venom ratio at which the coagulation time was increased three fold of that induced by 2MCD of the venom. Eleven batches of the antivenom were assayed for their lethality neutralizing activity (ED50) by the in vivo assay using mice as well as the developed in vitro assay. The correlation coefficient (r) between the in vitro neutralizing activities (ED) and in vivo neutralizing activities (ED50) was 0.957, (p value
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Development of an in vitro potency assay for antivenom against Malayan pit viper (Calloselasma rhodostoma).
Toxicon, 2014Co-Authors: Duangporn Pornmuttakun, Kavi RatanabanangkoonAbstract:An in vitro potency assay of antivenom against Malayan pit viper (Calloselasma rhodostoma, CR) has been developed. The assay is based on the neutralizing activity of the antivenom against the coagulant activity of the venom. The minimum coagulant dose (MCD) of CR venom was 22.12 ± 0.25 μg/ml. The coagulation time induced by 2MCD of the venom was used as the control for calculating the neutralizing activity of each batch of antivenom. The in vitro potency of antivenom, expressed as effective dose (ED), was the antivenom/venom ratio at which the coagulation time was increased three fold of that induced by 2MCD of the venom. Eleven batches of the antivenom were assayed for their lethality neutralizing activity (ED₅₀) by the in vivo assay using mice as well as the developed in vitro assay. The correlation coefficient (r) between the in vitro neutralizing activities (ED) and in vivo neutralizing activities (ED₅₀) was 0.957, (p value < 0.001). This simple and rapid in vitro assay of C. rhodostoma antivenom should be a good alternative method for the assessment of antivenom potency during the immunization program and fractionation process. The assay should be adaptable for use with antivenoms against other similar procoagulant venoms.
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a study of 225 malayan pit viper bites in thailand
Military Medicine, 2005Co-Authors: Nualnong Wongtongkam, Henry Wilde, Chitr Sitthiamorn, Kavi RatanabanangkoonAbstract:ABSTRACT This study evaluated factors affecting the severity of bite site necrosis and systemic symptoms resulting from envenomation among patients bitten by Malayan pit vipers (Calloselasma rhodostoma) in Thailand. We studied 145 victims prospectively. An additional 80 medical records were obtained for a retrospective study. Collected data included gender of the victims, anatomic locations of bites, where attacks took place, and predisposing factors and how they might have affected the clinical course. Most patients presented with minimal to moderate symptoms. Only eight patients required surgical wound debridement. None required amputation. However, 27 subjects developed permanently swollen limbs, presumably because of lymphatic or vascular injury. Significant coagulopathies were common (52.48%), and the only two deaths were attributable to intracranial hemorrhage. The improved clinical outcomes in the prospective and retrospective groups, compared with older series, might have been attributable to bett...
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Hyaluronidase inhibitors (sodium cromoglycate and sodium auro-thiomalate) reduce the local tissue damage and prolong the survival time of mice injected with Naja kaouthia and Calloselasma rhodostoma venoms.
Toxicon, 2003Co-Authors: Senee Yingprasertchai, Srisurat Bunyasrisawat, Kavi RatanabanangkoonAbstract:Experiments have been carried out to find potent inhibitors of hyaluronidases of Naja kaouthia (NK) and Calloselasma rhodostoma (CR) venoms with the aim of reducing local tissue damage and systemic toxicities caused by the venoms. Seven drugs/chemicals known to inhibit hyaluronidases were tested for their activity on venom enzymes. These were: sodium cromoglycate (SC), sodium aurothiomalate (SAT), apigenin, kaemferol, phenylbutazone, oxyphenbutazone and fenoprofen. The results showed that SC or SAT at 10 mM, completely inhibited the enzymes of both venoms. In in vivo experiments, SC or SAT, when incubated with NK venom prior to injection, significantly reduced edema and myonecrosis. In the case of CR venom, hemorrhage, in addition to edema and myonecrosis, was also significantly reduced. In the independent type experiment, SC or SAT were effective if injected within 1 min after the injection of venom. At longer time intervals of 3 and 10 min the inhibitors were effective in reducing some parameters of local tissue necrosis but the extent of inhibition was lower. SC and SAT at 256 and 195 μg/mouse, respectively, significantly prolonged the survival time of mice receiving lethal doses of NK. In the case of CR venoms, the two inhibitors not only prolonged the survival time but also prevented death of mice receiving lethal doses of the venom. The other inhibitors were poorly soluble in water and were studied only on enzyme inhibition and prolongation of survival time; they were mostly ineffective. Thus, SC and SAT when injected immediately at the sites of bites can reduce the systemic and local toxicity of NK and CR venoms. These results suggest that administration of these drugs at the site of venom injection may be useful in reducing venom-induced local tissue damage.
Rapepol Bavovada - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory effect of tea polyphenols on local tissue damage induced by snake venoms.
Phytotherapy research : PTR, 2010Co-Authors: Pimolpan Pithayanukul, J Leanpolchareanchai, Rapepol BavovadaAbstract:The methanolic extract of fresh tea leaves of Camellia sinensis L. (Theaceae) (CS) was assayed for its potential to inhibit enzymes with hydrolytic activity in Naja naja kaouthia Lesson (Elapidae) and Calloselasma rhodostoma Kuhl (Viperidae) venoms. These snake venom enzymes are responsible for the early effects of envenomation, such as local tissue damage and inflammation. The CS extract inhibited phospholipase A(2), proteases, hyaluronidase and L-amino acid oxidase in both venoms by in vitro neutralization and inhibited the hemorrhagic and the dermonecrotic activities of the venoms in vivo. It is suggested that the inhibitory potential of the CS extract against local tissue damage induced by snake venoms may be attributed to complexation and chelation between the venom proteins and the phenolic contents of the extract.
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Anti-necrosis potential of polyphenols against snake venoms
Immunopharmacology and Immunotoxicology, 2009Co-Authors: Jiraporn Leanpolchareanchai, Pimolpan Pithayanukul, Rapepol BavovadaAbstract:Polyphenols from the extracts of Areca catechu L. and Quercus infectoria Oliv. inhibited phospholipase A2, proteases, hyaluronidase and L-amino acid oxidase of Naja naja kaouthia Lesson (NK) and Calloselasma rhodostoma Kuhl (CR) venoms by in vitro tests. Both extracts inhibited the hemorrhagic activity of CR venom and the dermonecrotic activity of NK venom by in vivo tests. The inhibitory activity of plant polyphenols against local tissue necrosis induced by snake venoms may be caused by inhibition of inflammatory reactions, hemorrhage, and necrosis. The result implies the therapeutic potential of plant polyphenols against necrosis in snakebite victims.
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Inhibitory effect of tea polyphenols on local tissue damage induced by snake venoms.
Phytotherapy Research, 2009Co-Authors: Pimolpan Pithayanukul, Jiraporn Leanpolchareanchai, Rapepol BavovadaAbstract:The methanolic extract of fresh tea leaves of Camellia sinensis L. (Theaceae) (CS) was assayed for its potential to inhibit enzymes with hydrolytic activity in Naja naja kaouthia Lesson (Elapidae) and Calloselasma rhodostoma Kuhl (Viperidae) venoms. These snake venom enzymes are responsible for the early effects of envenomation, such as local tissue damage and inflammation. The CS extract inhibited phospholipase A2, proteases, hyaluronidase and l-amino acid oxidase in both venoms by in vitro neutralization and inhibited the hemorrhagic and the dermonecrotic activities of the venoms in vivo. It is suggested that the inhibitory potential of the CS extract against local tissue damage induced by snake venoms may be attributed to complexation and chelation between the venom proteins and the phenolic contents of the extract. Copyright © 2009 John Wiley & Sons, Ltd.
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molecular docking studies and anti enzymatic activities of thai mango seed kernel extract against snake venoms
Molecules, 2009Co-Authors: Jiraporn Leanpolchareanchai, Pimolpan Pithayanukul, Rapepol Bavovada, Patchreenart SaparpakornAbstract:Abstract: The ethanolic extract from seed kernels of Thai mango (MSKE) ( Mangifera indica L. cv. ‘Fahlun’) (Anacardiaceae) and its major phenolic principle (pentagalloyl glucopyranose) exhibited dose-dependent inhibitory effects on enzymatic activities of phospholipase A 2 (PLA 2 ), hyaluronidase and L -amino acid oxidase (LAAO) of Calloselasma rhodostoma (CR) and Naja naja kaouthia (NK) venoms by in vitro tests. The anti-hemorrhagic and anti-dermonecrotic activities of MSKE against both venoms were clearly supported by in vivo tests. Molecular docking studies indicated that the phenolic molecules of the MSKE could selectively bind to the active sites or their proximity, or modify conserved residues that are critical for the catalysis of PLA 2 , and selectively bind to the LAAO binding pocket of both CR and NK venoms and thereby inhibit their enzymatic activities. The results imply a potential use of MSKE against snake venoms. Keywords: Calloselasma rhodostoma ; Mangifera indica L.; Molecular docking study;
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Molecular docking studies and anti-enzymatic activities of Thai mango seed kernel extract against snake venoms.
Molecules, 2009Co-Authors: Jiraporn Leanpolchareanchai, Pimolpan Pithayanukul, Rapepol Bavovada, Patchreenart SaparpakornAbstract:The ethanolic extract from seed kernels of Thai mango (MSKE) (Mangifera indica L. cv. ‘Fahlun’) (Anacardiaceae) and its major phenolic principle (pentagalloyl glucopyranose) exhibited dose-dependent inhibitory effects on enzymatic activities of phospholipase A2 (PLA2), hyaluronidase and L-amino acid oxidase (LAAO) of Calloselasma rhodostoma (CR) and Naja naja kaouthia (NK)venoms by in vitro tests. The anti-hemorrhagic and anti-dermonecrotic activities of MSKE against both venoms were clearly supported by in vivo tests. Molecular docking studies indicated that the phenolic molecules of the MSKE could selectively bind to the active sites or their proximity, or modify conserved residues that are critical for the catalysis of PLA2, and selectively bind to the LAAO binding pocket of both CR and NK venoms and thereby inhibit their enzymatic activities. The results imply a potential use of MSKE against snake venoms.
Pimolpan Pithayanukul - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory effect of tea polyphenols on local tissue damage induced by snake venoms.
Phytotherapy research : PTR, 2010Co-Authors: Pimolpan Pithayanukul, J Leanpolchareanchai, Rapepol BavovadaAbstract:The methanolic extract of fresh tea leaves of Camellia sinensis L. (Theaceae) (CS) was assayed for its potential to inhibit enzymes with hydrolytic activity in Naja naja kaouthia Lesson (Elapidae) and Calloselasma rhodostoma Kuhl (Viperidae) venoms. These snake venom enzymes are responsible for the early effects of envenomation, such as local tissue damage and inflammation. The CS extract inhibited phospholipase A(2), proteases, hyaluronidase and L-amino acid oxidase in both venoms by in vitro neutralization and inhibited the hemorrhagic and the dermonecrotic activities of the venoms in vivo. It is suggested that the inhibitory potential of the CS extract against local tissue damage induced by snake venoms may be attributed to complexation and chelation between the venom proteins and the phenolic contents of the extract.
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Anti-necrosis potential of polyphenols against snake venoms
Immunopharmacology and Immunotoxicology, 2009Co-Authors: Jiraporn Leanpolchareanchai, Pimolpan Pithayanukul, Rapepol BavovadaAbstract:Polyphenols from the extracts of Areca catechu L. and Quercus infectoria Oliv. inhibited phospholipase A2, proteases, hyaluronidase and L-amino acid oxidase of Naja naja kaouthia Lesson (NK) and Calloselasma rhodostoma Kuhl (CR) venoms by in vitro tests. Both extracts inhibited the hemorrhagic activity of CR venom and the dermonecrotic activity of NK venom by in vivo tests. The inhibitory activity of plant polyphenols against local tissue necrosis induced by snake venoms may be caused by inhibition of inflammatory reactions, hemorrhage, and necrosis. The result implies the therapeutic potential of plant polyphenols against necrosis in snakebite victims.
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Inhibitory effect of tea polyphenols on local tissue damage induced by snake venoms.
Phytotherapy Research, 2009Co-Authors: Pimolpan Pithayanukul, Jiraporn Leanpolchareanchai, Rapepol BavovadaAbstract:The methanolic extract of fresh tea leaves of Camellia sinensis L. (Theaceae) (CS) was assayed for its potential to inhibit enzymes with hydrolytic activity in Naja naja kaouthia Lesson (Elapidae) and Calloselasma rhodostoma Kuhl (Viperidae) venoms. These snake venom enzymes are responsible for the early effects of envenomation, such as local tissue damage and inflammation. The CS extract inhibited phospholipase A2, proteases, hyaluronidase and l-amino acid oxidase in both venoms by in vitro neutralization and inhibited the hemorrhagic and the dermonecrotic activities of the venoms in vivo. It is suggested that the inhibitory potential of the CS extract against local tissue damage induced by snake venoms may be attributed to complexation and chelation between the venom proteins and the phenolic contents of the extract. Copyright © 2009 John Wiley & Sons, Ltd.
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molecular docking studies and anti enzymatic activities of thai mango seed kernel extract against snake venoms
Molecules, 2009Co-Authors: Jiraporn Leanpolchareanchai, Pimolpan Pithayanukul, Rapepol Bavovada, Patchreenart SaparpakornAbstract:Abstract: The ethanolic extract from seed kernels of Thai mango (MSKE) ( Mangifera indica L. cv. ‘Fahlun’) (Anacardiaceae) and its major phenolic principle (pentagalloyl glucopyranose) exhibited dose-dependent inhibitory effects on enzymatic activities of phospholipase A 2 (PLA 2 ), hyaluronidase and L -amino acid oxidase (LAAO) of Calloselasma rhodostoma (CR) and Naja naja kaouthia (NK) venoms by in vitro tests. The anti-hemorrhagic and anti-dermonecrotic activities of MSKE against both venoms were clearly supported by in vivo tests. Molecular docking studies indicated that the phenolic molecules of the MSKE could selectively bind to the active sites or their proximity, or modify conserved residues that are critical for the catalysis of PLA 2 , and selectively bind to the LAAO binding pocket of both CR and NK venoms and thereby inhibit their enzymatic activities. The results imply a potential use of MSKE against snake venoms. Keywords: Calloselasma rhodostoma ; Mangifera indica L.; Molecular docking study;
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Molecular docking studies and anti-enzymatic activities of Thai mango seed kernel extract against snake venoms.
Molecules, 2009Co-Authors: Jiraporn Leanpolchareanchai, Pimolpan Pithayanukul, Rapepol Bavovada, Patchreenart SaparpakornAbstract:The ethanolic extract from seed kernels of Thai mango (MSKE) (Mangifera indica L. cv. ‘Fahlun’) (Anacardiaceae) and its major phenolic principle (pentagalloyl glucopyranose) exhibited dose-dependent inhibitory effects on enzymatic activities of phospholipase A2 (PLA2), hyaluronidase and L-amino acid oxidase (LAAO) of Calloselasma rhodostoma (CR) and Naja naja kaouthia (NK)venoms by in vitro tests. The anti-hemorrhagic and anti-dermonecrotic activities of MSKE against both venoms were clearly supported by in vivo tests. Molecular docking studies indicated that the phenolic molecules of the MSKE could selectively bind to the active sites or their proximity, or modify conserved residues that are critical for the catalysis of PLA2, and selectively bind to the LAAO binding pocket of both CR and NK venoms and thereby inhibit their enzymatic activities. The results imply a potential use of MSKE against snake venoms.
Jiraporn Leanpolchareanchai - One of the best experts on this subject based on the ideXlab platform.
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Anti-necrosis potential of polyphenols against snake venoms
Immunopharmacology and Immunotoxicology, 2009Co-Authors: Jiraporn Leanpolchareanchai, Pimolpan Pithayanukul, Rapepol BavovadaAbstract:Polyphenols from the extracts of Areca catechu L. and Quercus infectoria Oliv. inhibited phospholipase A2, proteases, hyaluronidase and L-amino acid oxidase of Naja naja kaouthia Lesson (NK) and Calloselasma rhodostoma Kuhl (CR) venoms by in vitro tests. Both extracts inhibited the hemorrhagic activity of CR venom and the dermonecrotic activity of NK venom by in vivo tests. The inhibitory activity of plant polyphenols against local tissue necrosis induced by snake venoms may be caused by inhibition of inflammatory reactions, hemorrhage, and necrosis. The result implies the therapeutic potential of plant polyphenols against necrosis in snakebite victims.
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Inhibitory effect of tea polyphenols on local tissue damage induced by snake venoms.
Phytotherapy Research, 2009Co-Authors: Pimolpan Pithayanukul, Jiraporn Leanpolchareanchai, Rapepol BavovadaAbstract:The methanolic extract of fresh tea leaves of Camellia sinensis L. (Theaceae) (CS) was assayed for its potential to inhibit enzymes with hydrolytic activity in Naja naja kaouthia Lesson (Elapidae) and Calloselasma rhodostoma Kuhl (Viperidae) venoms. These snake venom enzymes are responsible for the early effects of envenomation, such as local tissue damage and inflammation. The CS extract inhibited phospholipase A2, proteases, hyaluronidase and l-amino acid oxidase in both venoms by in vitro neutralization and inhibited the hemorrhagic and the dermonecrotic activities of the venoms in vivo. It is suggested that the inhibitory potential of the CS extract against local tissue damage induced by snake venoms may be attributed to complexation and chelation between the venom proteins and the phenolic contents of the extract. Copyright © 2009 John Wiley & Sons, Ltd.
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molecular docking studies and anti enzymatic activities of thai mango seed kernel extract against snake venoms
Molecules, 2009Co-Authors: Jiraporn Leanpolchareanchai, Pimolpan Pithayanukul, Rapepol Bavovada, Patchreenart SaparpakornAbstract:Abstract: The ethanolic extract from seed kernels of Thai mango (MSKE) ( Mangifera indica L. cv. ‘Fahlun’) (Anacardiaceae) and its major phenolic principle (pentagalloyl glucopyranose) exhibited dose-dependent inhibitory effects on enzymatic activities of phospholipase A 2 (PLA 2 ), hyaluronidase and L -amino acid oxidase (LAAO) of Calloselasma rhodostoma (CR) and Naja naja kaouthia (NK) venoms by in vitro tests. The anti-hemorrhagic and anti-dermonecrotic activities of MSKE against both venoms were clearly supported by in vivo tests. Molecular docking studies indicated that the phenolic molecules of the MSKE could selectively bind to the active sites or their proximity, or modify conserved residues that are critical for the catalysis of PLA 2 , and selectively bind to the LAAO binding pocket of both CR and NK venoms and thereby inhibit their enzymatic activities. The results imply a potential use of MSKE against snake venoms. Keywords: Calloselasma rhodostoma ; Mangifera indica L.; Molecular docking study;
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Molecular docking studies and anti-enzymatic activities of Thai mango seed kernel extract against snake venoms.
Molecules, 2009Co-Authors: Jiraporn Leanpolchareanchai, Pimolpan Pithayanukul, Rapepol Bavovada, Patchreenart SaparpakornAbstract:The ethanolic extract from seed kernels of Thai mango (MSKE) (Mangifera indica L. cv. ‘Fahlun’) (Anacardiaceae) and its major phenolic principle (pentagalloyl glucopyranose) exhibited dose-dependent inhibitory effects on enzymatic activities of phospholipase A2 (PLA2), hyaluronidase and L-amino acid oxidase (LAAO) of Calloselasma rhodostoma (CR) and Naja naja kaouthia (NK)venoms by in vitro tests. The anti-hemorrhagic and anti-dermonecrotic activities of MSKE against both venoms were clearly supported by in vivo tests. Molecular docking studies indicated that the phenolic molecules of the MSKE could selectively bind to the active sites or their proximity, or modify conserved residues that are critical for the catalysis of PLA2, and selectively bind to the LAAO binding pocket of both CR and NK venoms and thereby inhibit their enzymatic activities. The results imply a potential use of MSKE against snake venoms.
Patchreenart Saparpakorn - One of the best experts on this subject based on the ideXlab platform.
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molecular docking studies and anti enzymatic activities of thai mango seed kernel extract against snake venoms
Molecules, 2009Co-Authors: Jiraporn Leanpolchareanchai, Pimolpan Pithayanukul, Rapepol Bavovada, Patchreenart SaparpakornAbstract:Abstract: The ethanolic extract from seed kernels of Thai mango (MSKE) ( Mangifera indica L. cv. ‘Fahlun’) (Anacardiaceae) and its major phenolic principle (pentagalloyl glucopyranose) exhibited dose-dependent inhibitory effects on enzymatic activities of phospholipase A 2 (PLA 2 ), hyaluronidase and L -amino acid oxidase (LAAO) of Calloselasma rhodostoma (CR) and Naja naja kaouthia (NK) venoms by in vitro tests. The anti-hemorrhagic and anti-dermonecrotic activities of MSKE against both venoms were clearly supported by in vivo tests. Molecular docking studies indicated that the phenolic molecules of the MSKE could selectively bind to the active sites or their proximity, or modify conserved residues that are critical for the catalysis of PLA 2 , and selectively bind to the LAAO binding pocket of both CR and NK venoms and thereby inhibit their enzymatic activities. The results imply a potential use of MSKE against snake venoms. Keywords: Calloselasma rhodostoma ; Mangifera indica L.; Molecular docking study;
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Molecular docking studies and anti-enzymatic activities of Thai mango seed kernel extract against snake venoms.
Molecules, 2009Co-Authors: Jiraporn Leanpolchareanchai, Pimolpan Pithayanukul, Rapepol Bavovada, Patchreenart SaparpakornAbstract:The ethanolic extract from seed kernels of Thai mango (MSKE) (Mangifera indica L. cv. ‘Fahlun’) (Anacardiaceae) and its major phenolic principle (pentagalloyl glucopyranose) exhibited dose-dependent inhibitory effects on enzymatic activities of phospholipase A2 (PLA2), hyaluronidase and L-amino acid oxidase (LAAO) of Calloselasma rhodostoma (CR) and Naja naja kaouthia (NK)venoms by in vitro tests. The anti-hemorrhagic and anti-dermonecrotic activities of MSKE against both venoms were clearly supported by in vivo tests. Molecular docking studies indicated that the phenolic molecules of the MSKE could selectively bind to the active sites or their proximity, or modify conserved residues that are critical for the catalysis of PLA2, and selectively bind to the LAAO binding pocket of both CR and NK venoms and thereby inhibit their enzymatic activities. The results imply a potential use of MSKE against snake venoms.
