The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform
Mohammad Hossein Mirjalili - One of the best experts on this subject based on the ideXlab platform.
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improved effects of polyethylene glycol on the growth antioxidative enzymes activity and taxanes production in a taxus baccata l Callus Culture
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Marziyeh Sarmadi, Naser Karimi, Javier Palazon, Alireza Ghassempour, Mohammad Hossein MirjaliliAbstract:Application of abiotic stress-inducing elicitors is one of the effective strategies in order to increase the production of secondary metabolites in plant tissues. Here, the effect of in vitro polyethylene glycol (PEG)-induced drought stress on morphological and biochemical changes as well as the production of paclitaxel and 10-deacetyl baccatin III in a Taxus baccata Callus Culture was investigated. The results showed that increased PEG concentration in the Culture medium enhanced the levels of H2O2 and the membrane lipid peroxidation in the calli. The activity of catalase and guaiacol peroxidase as antioxidant responses were increased significantly in all PEG concentrations. At 6% of PEG concentration, the levels of fresh weight (FW) and dry weight (DW) in the calli were declined significantly by dropping in the amount of water content in the calli and rising the level of oxidative stress, and the morphological changes occurred following the increased browning index of the calli. However, the PEG at low to moderate concentrations reduced the accumulation and oxidation of phenolic compounds in the calli through the absorption of phenolic compounds and the reduction in the activity of the polyphenol oxidase. As a result, improved growth and viability of the calli resulted in the enhanced levels of FW and DW at the concentrations of 1% up to 3% (w/v) PEG. The highest contents of 10-deacetyl baccatin III and taxol were obtained at concentrations of 2% and 3% PEG, respectively. The concentration of 3% PEG increased the production of taxol by 2.5 times more than the control. In conclusion, PEG can be proposed as an effective elicitor in the production of taxanes in the Taxus cell and tissue Cultures.
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the effects of salicylic acid and glucose on biochemical traits and taxane production in a taxus baccata Callus Culture
Plant Physiology and Biochemistry, 2018Co-Authors: Marziyeh Sarmadi, Naser Karimi, Javier Palazon, Alireza Ghassempour, Mohammad Hossein MirjaliliAbstract:Abstract The combined use of elicitors can be an effective way to increase the production of secondary metabolites (SMs) in plant cell, tissue and organ Cultures. This study investigated the effects of a salicylic acid (SA) pretreatment and different glucose levels on the growth, biochemical traits and taxane production in a Taxus baccata Callus Culture. For this purpose, after a pretreatment with SA (5 μM), three-month-old calli were Cultured on B5 medium fortified with different concentrations of glucose (0, 0.5, 1, 2 and 3%), and they were compared with calli Cultured on a B5 medium supplemented only with glucose. When the calli were harvested at 21 days, their fresh weight (g), dry weight (g) and cell viability (%) had decreased significantly (p
Marziyeh Sarmadi - One of the best experts on this subject based on the ideXlab platform.
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improved effects of polyethylene glycol on the growth antioxidative enzymes activity and taxanes production in a taxus baccata l Callus Culture
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Marziyeh Sarmadi, Naser Karimi, Javier Palazon, Alireza Ghassempour, Mohammad Hossein MirjaliliAbstract:Application of abiotic stress-inducing elicitors is one of the effective strategies in order to increase the production of secondary metabolites in plant tissues. Here, the effect of in vitro polyethylene glycol (PEG)-induced drought stress on morphological and biochemical changes as well as the production of paclitaxel and 10-deacetyl baccatin III in a Taxus baccata Callus Culture was investigated. The results showed that increased PEG concentration in the Culture medium enhanced the levels of H2O2 and the membrane lipid peroxidation in the calli. The activity of catalase and guaiacol peroxidase as antioxidant responses were increased significantly in all PEG concentrations. At 6% of PEG concentration, the levels of fresh weight (FW) and dry weight (DW) in the calli were declined significantly by dropping in the amount of water content in the calli and rising the level of oxidative stress, and the morphological changes occurred following the increased browning index of the calli. However, the PEG at low to moderate concentrations reduced the accumulation and oxidation of phenolic compounds in the calli through the absorption of phenolic compounds and the reduction in the activity of the polyphenol oxidase. As a result, improved growth and viability of the calli resulted in the enhanced levels of FW and DW at the concentrations of 1% up to 3% (w/v) PEG. The highest contents of 10-deacetyl baccatin III and taxol were obtained at concentrations of 2% and 3% PEG, respectively. The concentration of 3% PEG increased the production of taxol by 2.5 times more than the control. In conclusion, PEG can be proposed as an effective elicitor in the production of taxanes in the Taxus cell and tissue Cultures.
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the effects of salicylic acid and glucose on biochemical traits and taxane production in a taxus baccata Callus Culture
Plant Physiology and Biochemistry, 2018Co-Authors: Marziyeh Sarmadi, Naser Karimi, Javier Palazon, Alireza Ghassempour, Mohammad Hossein MirjaliliAbstract:Abstract The combined use of elicitors can be an effective way to increase the production of secondary metabolites (SMs) in plant cell, tissue and organ Cultures. This study investigated the effects of a salicylic acid (SA) pretreatment and different glucose levels on the growth, biochemical traits and taxane production in a Taxus baccata Callus Culture. For this purpose, after a pretreatment with SA (5 μM), three-month-old calli were Cultured on B5 medium fortified with different concentrations of glucose (0, 0.5, 1, 2 and 3%), and they were compared with calli Cultured on a B5 medium supplemented only with glucose. When the calli were harvested at 21 days, their fresh weight (g), dry weight (g) and cell viability (%) had decreased significantly (p
Alireza Ghassempour - One of the best experts on this subject based on the ideXlab platform.
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improved effects of polyethylene glycol on the growth antioxidative enzymes activity and taxanes production in a taxus baccata l Callus Culture
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Marziyeh Sarmadi, Naser Karimi, Javier Palazon, Alireza Ghassempour, Mohammad Hossein MirjaliliAbstract:Application of abiotic stress-inducing elicitors is one of the effective strategies in order to increase the production of secondary metabolites in plant tissues. Here, the effect of in vitro polyethylene glycol (PEG)-induced drought stress on morphological and biochemical changes as well as the production of paclitaxel and 10-deacetyl baccatin III in a Taxus baccata Callus Culture was investigated. The results showed that increased PEG concentration in the Culture medium enhanced the levels of H2O2 and the membrane lipid peroxidation in the calli. The activity of catalase and guaiacol peroxidase as antioxidant responses were increased significantly in all PEG concentrations. At 6% of PEG concentration, the levels of fresh weight (FW) and dry weight (DW) in the calli were declined significantly by dropping in the amount of water content in the calli and rising the level of oxidative stress, and the morphological changes occurred following the increased browning index of the calli. However, the PEG at low to moderate concentrations reduced the accumulation and oxidation of phenolic compounds in the calli through the absorption of phenolic compounds and the reduction in the activity of the polyphenol oxidase. As a result, improved growth and viability of the calli resulted in the enhanced levels of FW and DW at the concentrations of 1% up to 3% (w/v) PEG. The highest contents of 10-deacetyl baccatin III and taxol were obtained at concentrations of 2% and 3% PEG, respectively. The concentration of 3% PEG increased the production of taxol by 2.5 times more than the control. In conclusion, PEG can be proposed as an effective elicitor in the production of taxanes in the Taxus cell and tissue Cultures.
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the effects of salicylic acid and glucose on biochemical traits and taxane production in a taxus baccata Callus Culture
Plant Physiology and Biochemistry, 2018Co-Authors: Marziyeh Sarmadi, Naser Karimi, Javier Palazon, Alireza Ghassempour, Mohammad Hossein MirjaliliAbstract:Abstract The combined use of elicitors can be an effective way to increase the production of secondary metabolites (SMs) in plant cell, tissue and organ Cultures. This study investigated the effects of a salicylic acid (SA) pretreatment and different glucose levels on the growth, biochemical traits and taxane production in a Taxus baccata Callus Culture. For this purpose, after a pretreatment with SA (5 μM), three-month-old calli were Cultured on B5 medium fortified with different concentrations of glucose (0, 0.5, 1, 2 and 3%), and they were compared with calli Cultured on a B5 medium supplemented only with glucose. When the calli were harvested at 21 days, their fresh weight (g), dry weight (g) and cell viability (%) had decreased significantly (p
Javier Palazon - One of the best experts on this subject based on the ideXlab platform.
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improved effects of polyethylene glycol on the growth antioxidative enzymes activity and taxanes production in a taxus baccata l Callus Culture
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Marziyeh Sarmadi, Naser Karimi, Javier Palazon, Alireza Ghassempour, Mohammad Hossein MirjaliliAbstract:Application of abiotic stress-inducing elicitors is one of the effective strategies in order to increase the production of secondary metabolites in plant tissues. Here, the effect of in vitro polyethylene glycol (PEG)-induced drought stress on morphological and biochemical changes as well as the production of paclitaxel and 10-deacetyl baccatin III in a Taxus baccata Callus Culture was investigated. The results showed that increased PEG concentration in the Culture medium enhanced the levels of H2O2 and the membrane lipid peroxidation in the calli. The activity of catalase and guaiacol peroxidase as antioxidant responses were increased significantly in all PEG concentrations. At 6% of PEG concentration, the levels of fresh weight (FW) and dry weight (DW) in the calli were declined significantly by dropping in the amount of water content in the calli and rising the level of oxidative stress, and the morphological changes occurred following the increased browning index of the calli. However, the PEG at low to moderate concentrations reduced the accumulation and oxidation of phenolic compounds in the calli through the absorption of phenolic compounds and the reduction in the activity of the polyphenol oxidase. As a result, improved growth and viability of the calli resulted in the enhanced levels of FW and DW at the concentrations of 1% up to 3% (w/v) PEG. The highest contents of 10-deacetyl baccatin III and taxol were obtained at concentrations of 2% and 3% PEG, respectively. The concentration of 3% PEG increased the production of taxol by 2.5 times more than the control. In conclusion, PEG can be proposed as an effective elicitor in the production of taxanes in the Taxus cell and tissue Cultures.
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the effects of salicylic acid and glucose on biochemical traits and taxane production in a taxus baccata Callus Culture
Plant Physiology and Biochemistry, 2018Co-Authors: Marziyeh Sarmadi, Naser Karimi, Javier Palazon, Alireza Ghassempour, Mohammad Hossein MirjaliliAbstract:Abstract The combined use of elicitors can be an effective way to increase the production of secondary metabolites (SMs) in plant cell, tissue and organ Cultures. This study investigated the effects of a salicylic acid (SA) pretreatment and different glucose levels on the growth, biochemical traits and taxane production in a Taxus baccata Callus Culture. For this purpose, after a pretreatment with SA (5 μM), three-month-old calli were Cultured on B5 medium fortified with different concentrations of glucose (0, 0.5, 1, 2 and 3%), and they were compared with calli Cultured on a B5 medium supplemented only with glucose. When the calli were harvested at 21 days, their fresh weight (g), dry weight (g) and cell viability (%) had decreased significantly (p
Naser Karimi - One of the best experts on this subject based on the ideXlab platform.
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improved effects of polyethylene glycol on the growth antioxidative enzymes activity and taxanes production in a taxus baccata l Callus Culture
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Marziyeh Sarmadi, Naser Karimi, Javier Palazon, Alireza Ghassempour, Mohammad Hossein MirjaliliAbstract:Application of abiotic stress-inducing elicitors is one of the effective strategies in order to increase the production of secondary metabolites in plant tissues. Here, the effect of in vitro polyethylene glycol (PEG)-induced drought stress on morphological and biochemical changes as well as the production of paclitaxel and 10-deacetyl baccatin III in a Taxus baccata Callus Culture was investigated. The results showed that increased PEG concentration in the Culture medium enhanced the levels of H2O2 and the membrane lipid peroxidation in the calli. The activity of catalase and guaiacol peroxidase as antioxidant responses were increased significantly in all PEG concentrations. At 6% of PEG concentration, the levels of fresh weight (FW) and dry weight (DW) in the calli were declined significantly by dropping in the amount of water content in the calli and rising the level of oxidative stress, and the morphological changes occurred following the increased browning index of the calli. However, the PEG at low to moderate concentrations reduced the accumulation and oxidation of phenolic compounds in the calli through the absorption of phenolic compounds and the reduction in the activity of the polyphenol oxidase. As a result, improved growth and viability of the calli resulted in the enhanced levels of FW and DW at the concentrations of 1% up to 3% (w/v) PEG. The highest contents of 10-deacetyl baccatin III and taxol were obtained at concentrations of 2% and 3% PEG, respectively. The concentration of 3% PEG increased the production of taxol by 2.5 times more than the control. In conclusion, PEG can be proposed as an effective elicitor in the production of taxanes in the Taxus cell and tissue Cultures.
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the effects of salicylic acid and glucose on biochemical traits and taxane production in a taxus baccata Callus Culture
Plant Physiology and Biochemistry, 2018Co-Authors: Marziyeh Sarmadi, Naser Karimi, Javier Palazon, Alireza Ghassempour, Mohammad Hossein MirjaliliAbstract:Abstract The combined use of elicitors can be an effective way to increase the production of secondary metabolites (SMs) in plant cell, tissue and organ Cultures. This study investigated the effects of a salicylic acid (SA) pretreatment and different glucose levels on the growth, biochemical traits and taxane production in a Taxus baccata Callus Culture. For this purpose, after a pretreatment with SA (5 μM), three-month-old calli were Cultured on B5 medium fortified with different concentrations of glucose (0, 0.5, 1, 2 and 3%), and they were compared with calli Cultured on a B5 medium supplemented only with glucose. When the calli were harvested at 21 days, their fresh weight (g), dry weight (g) and cell viability (%) had decreased significantly (p
