The Experts below are selected from a list of 11010 Experts worldwide ranked by ideXlab platform
Renaud Maillard - One of the best experts on this subject based on the ideXlab platform.
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“Candidatus anaplasma camelii” in one-humped camels (Camelus dromedarius) in Morocco: a novel and emerging Anaplasma species?
Infectious Diseases of Poverty, 2017Co-Authors: Hicham Ait Lbacha, Zaid Zouagui, Said Alali, Abdelkbir Rhalem, Elisabeth Petit, Marie Julie Ducrotoy, Henri-jean Boulouis, Renaud MaillardAbstract:Background There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential. The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected. Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies, undertaken in Tunisia, Saudia Arabia and China, have used molecular diagnostics. The present work characterises Anaplasmataceae strains circulating in the Camelus dromedarius reservoir in Morocco using PCR. Methods Camels ( n = 106) were randomly sampled from 6 regions representing different agro-ecological areas in southern Morocco. Whole blood was collected and screened using PCR methods targeting the gene groEL . Anaplasmataceae strains were characterised by sequence analysis of the gene groEL . Results A total of 39.62% (42/106) camels screened were positive for Anaplasmataceae spp. GenBank BLAST analysis of five positive sequenced samples revealed that all strains were 100% identical to “ Candidatus Anaplasma camelii”. Phylogenetic investigation and genetic characterisation of the aligned segment (650 bp) of the gene groEL confirmed high similarity with A. platys . Conclusion This study demonstrates the circulation of a previously unidentified species of the genus Anaplasma in Morocco which is genetically close to the agent causing canine anaplasmosis but whose main reservoir is thought to be Camelus dromedarius . Trial registration number This study is not a clinical trial and therefore a trial registration number does not apply.
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candidatus anaplasma camelii in one humped camels Camelus dromedarius in morocco a novel and emerging anaplasma species
Infectious Diseases of Poverty, 2017Co-Authors: Hicham Ait Lbacha, Zaid Zouagui, Said Alali, Abdelkbir Rhalem, Elisabeth Petit, Marie Julie Ducrotoy, Henri-jean Boulouis, Renaud MaillardAbstract:There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential. The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected. Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies, undertaken in Tunisia, Saudia Arabia and China, have used molecular diagnostics. The present work characterises Anaplasmataceae strains circulating in the Camelus dromedarius reservoir in Morocco using PCR. Camels (n = 106) were randomly sampled from 6 regions representing different agro-ecological areas in southern Morocco. Whole blood was collected and screened using PCR methods targeting the gene groEL. Anaplasmataceae strains were characterised by sequence analysis of the gene groEL. A total of 39.62% (42/106) camels screened were positive for Anaplasmataceae spp. GenBank BLAST analysis of five positive sequenced samples revealed that all strains were 100% identical to “Candidatus Anaplasma camelii”. Phylogenetic investigation and genetic characterisation of the aligned segment (650 bp) of the gene groEL confirmed high similarity with A. platys. This study demonstrates the circulation of a previously unidentified species of the genus Anaplasma in Morocco which is genetically close to the agent causing canine anaplasmosis but whose main reservoir is thought to be Camelus dromedarius. This study is not a clinical trial and therefore a trial registration number does not apply.
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“Candidatus anaplasma camelii” in one-humped camels (Camelus dromedarius) in Morocco: a novel and emerging Anaplasma species?
Infectious Diseases of Poverty, 2017Co-Authors: Hicham Ait Lbacha, Zaid Zouagui, Said Alali, Abdelkbir Rhalem, Elisabeth Petit, Marie Julie Ducrotoy, Henri-jean Boulouis, Renaud MaillardAbstract:Background There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential. The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected. Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies, undertaken in Tunisia, Saudia Arabia and China, have used molecular diagnostics. The present work characterises Anaplasmataceae strains circulating in the Camelus dromedarius reservoir in Morocco using PCR. Methods Camels ( n = 106) were randomly sampled from 6 regions representing different agro-ecological areas in southern Morocco. Whole blood was collected and screened using PCR methods targeting the gene groEL . Anaplasmataceae strains were characterised by sequence analysis of the gene groEL . Results A total of 39.62% (42/106) camels screened were positive for Anaplasmataceae spp. GenBank BLAST analysis of five positive sequenced samples revealed that all strains were 100% identical to “ Candidatus Anaplasma camelii”. Phylogenetic investigation and genetic characterisation of the aligned segment (650 bp) of the gene groEL confirmed high similarity with A. platys . Conclusion This study demonstrates the circulation of a previously unidentified species of the genus Anaplasma in Morocco which is genetically close to the agent causing canine anaplasmosis but whose main reservoir is thought to be Camelus dromedarius . Trial registration number This study is not a clinical trial and therefore a trial registration number does not apply.
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Candidatus anaplasma camelii" in onehumped camels (Camelus dromedarius) in Morocco: a novel and emerging anaplasma species?
Infectious Diseases of Poverty, 2017Co-Authors: Hicham Ait Lbacha, Zaid Zouagui, Said Alali, Abdelkbir Rhalem, Elisabeth Petit, Marie Julie Ducrotoy, Henri-jean Boulouis, Renaud MaillardAbstract:Background: There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential. The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected. Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies, undertaken in Tunisia, Saudia Arabia and China, have used molecular diagnostics. The present work characterises Anaplasmataceae strains circulating in the Camelus dromedarius reservoir in Morocco using PCR. Methods: Camels (n = 106) were randomly sampled from 6 regions representing different agro-ecological areas in southern Morocco. Whole blood was collected and screened using PCR methods targeting the gene groEL. Anaplasmataceae strains were characterised by sequence analysis of the gene groEL. Results: A total of 39.62% (42/106) camels screened were positive for Anaplasmataceae spp. GenBank BLAST analysis of five positive sequenced samples revealed that all strains were 100% identical to "Candidatus Anaplasma camelii". Phylogenetic investigation and genetic characterisation of the aligned segment (650 bp) of the gene groEL confirmed high similarity with A. platys. Conclusion: This study demonstrates the circulation of a previously unidentified species of the genus Anaplasma in Morocco which is genetically close to the agent causing canine anaplasmosis but whose main reservoir is thought to be Camelus dromedarius.
Sharon L Deem - One of the best experts on this subject based on the ideXlab platform.
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A Review of Zoonotic Pathogens of Dromedary Camels
EcoHealth, 2019Co-Authors: Sophie Zhu, Dawn Zimmerman, Sharon L DeemAbstract:Dromedary, or one-humped, camels Camelus dromedarius are an almost exclusively domesticated species that are common in arid areas as both beasts of burden and production animals for meat and milk. Currently, there are approximately 30 million dromedary camels, with highest numbers in Africa and the Middle East. The hardiness of camels in arid regions has made humans more dependent on them, especially as a stable protein source. Camels also carry and may transmit disease-causing agents to humans and other animals. The ability for camels to act as a point source or vector for disease is a concern due to increasing human demands for meat, lack of biosafety and biosecurity protocols in many regions, and a growth in the interface with wildlife as camel herds become sympatric with non-domestic species. We conducted a literature review of camel-borne zoonotic diseases and found that the majority of publications (65%) focused on Middle East respiratory syndrome (MERS), brucellosis, Echinococcus granulosus , and Rift Valley fever. The high fatality from MERS outbreaks during 2012–2016 elicited an immediate response from the research community as demonstrated by a surge of MERS-related publications. However, we contend that other camel-borne diseases such as Yersinia pestis , Coxiella burnetii , and Crimean–Congo hemorrhagic fever are just as important to include in surveillance efforts. Camel populations, particularly in sub-Saharan Africa, are increasing exponentially in response to prolonged droughts, and thus, the risk of zoonoses increases as well. In this review, we provide an overview of the major zoonotic diseases present in dromedary camels, their risk to humans, and recommendations to minimize spillover events.
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serosurvey of coxiella burnetii q fever in dromedary camels Camelus dromedarius in laikipia county kenya
Zoonoses and Public Health, 2017Co-Authors: A S Browne, Eric M Fevre, Margaret F Kinnaird, Dishon Muloi, C A Wang, P S Larsen, T Obrien, Sharon L DeemAbstract:Dromedary camels (Camelus dromedarius) are an important protein source for people in semi-arid and arid regions of Africa. In Kenya, camel populations have grown dramatically in the past few decades resulting in the potential for increased disease transmission between humans and camels. An estimated four million Kenyans drink unpasteurized camel milk, which poses a disease risk. We evaluated the seroprevalence of a significant zoonotic pathogen, Coxiella burnetii (Q fever), among 334 camels from nine herds in Laikipia County, Kenya. Serum testing revealed 18.6% positive seroprevalence of Coxiella burnetii (n = 344). Increasing camel age was positively associated with C. burnetii seroprevalence (OR = 5.36). Our study confirmed that camels living in Laikipia County, Kenya, have been exposed to the zoonotic pathogen, C. burnetii. Further research to evaluate the role of camels in disease transmission to other livestock, wildlife and humans in Kenya should be conducted.
Marie Julie Ducrotoy - One of the best experts on this subject based on the ideXlab platform.
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“Candidatus anaplasma camelii” in one-humped camels (Camelus dromedarius) in Morocco: a novel and emerging Anaplasma species?
Infectious Diseases of Poverty, 2017Co-Authors: Hicham Ait Lbacha, Zaid Zouagui, Said Alali, Abdelkbir Rhalem, Elisabeth Petit, Marie Julie Ducrotoy, Henri-jean Boulouis, Renaud MaillardAbstract:Background There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential. The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected. Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies, undertaken in Tunisia, Saudia Arabia and China, have used molecular diagnostics. The present work characterises Anaplasmataceae strains circulating in the Camelus dromedarius reservoir in Morocco using PCR. Methods Camels ( n = 106) were randomly sampled from 6 regions representing different agro-ecological areas in southern Morocco. Whole blood was collected and screened using PCR methods targeting the gene groEL . Anaplasmataceae strains were characterised by sequence analysis of the gene groEL . Results A total of 39.62% (42/106) camels screened were positive for Anaplasmataceae spp. GenBank BLAST analysis of five positive sequenced samples revealed that all strains were 100% identical to “ Candidatus Anaplasma camelii”. Phylogenetic investigation and genetic characterisation of the aligned segment (650 bp) of the gene groEL confirmed high similarity with A. platys . Conclusion This study demonstrates the circulation of a previously unidentified species of the genus Anaplasma in Morocco which is genetically close to the agent causing canine anaplasmosis but whose main reservoir is thought to be Camelus dromedarius . Trial registration number This study is not a clinical trial and therefore a trial registration number does not apply.
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candidatus anaplasma camelii in one humped camels Camelus dromedarius in morocco a novel and emerging anaplasma species
Infectious Diseases of Poverty, 2017Co-Authors: Hicham Ait Lbacha, Zaid Zouagui, Said Alali, Abdelkbir Rhalem, Elisabeth Petit, Marie Julie Ducrotoy, Henri-jean Boulouis, Renaud MaillardAbstract:There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential. The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected. Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies, undertaken in Tunisia, Saudia Arabia and China, have used molecular diagnostics. The present work characterises Anaplasmataceae strains circulating in the Camelus dromedarius reservoir in Morocco using PCR. Camels (n = 106) were randomly sampled from 6 regions representing different agro-ecological areas in southern Morocco. Whole blood was collected and screened using PCR methods targeting the gene groEL. Anaplasmataceae strains were characterised by sequence analysis of the gene groEL. A total of 39.62% (42/106) camels screened were positive for Anaplasmataceae spp. GenBank BLAST analysis of five positive sequenced samples revealed that all strains were 100% identical to “Candidatus Anaplasma camelii”. Phylogenetic investigation and genetic characterisation of the aligned segment (650 bp) of the gene groEL confirmed high similarity with A. platys. This study demonstrates the circulation of a previously unidentified species of the genus Anaplasma in Morocco which is genetically close to the agent causing canine anaplasmosis but whose main reservoir is thought to be Camelus dromedarius. This study is not a clinical trial and therefore a trial registration number does not apply.
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“Candidatus anaplasma camelii” in one-humped camels (Camelus dromedarius) in Morocco: a novel and emerging Anaplasma species?
Infectious Diseases of Poverty, 2017Co-Authors: Hicham Ait Lbacha, Zaid Zouagui, Said Alali, Abdelkbir Rhalem, Elisabeth Petit, Marie Julie Ducrotoy, Henri-jean Boulouis, Renaud MaillardAbstract:Background There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential. The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected. Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies, undertaken in Tunisia, Saudia Arabia and China, have used molecular diagnostics. The present work characterises Anaplasmataceae strains circulating in the Camelus dromedarius reservoir in Morocco using PCR. Methods Camels ( n = 106) were randomly sampled from 6 regions representing different agro-ecological areas in southern Morocco. Whole blood was collected and screened using PCR methods targeting the gene groEL . Anaplasmataceae strains were characterised by sequence analysis of the gene groEL . Results A total of 39.62% (42/106) camels screened were positive for Anaplasmataceae spp. GenBank BLAST analysis of five positive sequenced samples revealed that all strains were 100% identical to “ Candidatus Anaplasma camelii”. Phylogenetic investigation and genetic characterisation of the aligned segment (650 bp) of the gene groEL confirmed high similarity with A. platys . Conclusion This study demonstrates the circulation of a previously unidentified species of the genus Anaplasma in Morocco which is genetically close to the agent causing canine anaplasmosis but whose main reservoir is thought to be Camelus dromedarius . Trial registration number This study is not a clinical trial and therefore a trial registration number does not apply.
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Candidatus anaplasma camelii" in onehumped camels (Camelus dromedarius) in Morocco: a novel and emerging anaplasma species?
Infectious Diseases of Poverty, 2017Co-Authors: Hicham Ait Lbacha, Zaid Zouagui, Said Alali, Abdelkbir Rhalem, Elisabeth Petit, Marie Julie Ducrotoy, Henri-jean Boulouis, Renaud MaillardAbstract:Background: There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential. The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected. Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies, undertaken in Tunisia, Saudia Arabia and China, have used molecular diagnostics. The present work characterises Anaplasmataceae strains circulating in the Camelus dromedarius reservoir in Morocco using PCR. Methods: Camels (n = 106) were randomly sampled from 6 regions representing different agro-ecological areas in southern Morocco. Whole blood was collected and screened using PCR methods targeting the gene groEL. Anaplasmataceae strains were characterised by sequence analysis of the gene groEL. Results: A total of 39.62% (42/106) camels screened were positive for Anaplasmataceae spp. GenBank BLAST analysis of five positive sequenced samples revealed that all strains were 100% identical to "Candidatus Anaplasma camelii". Phylogenetic investigation and genetic characterisation of the aligned segment (650 bp) of the gene groEL confirmed high similarity with A. platys. Conclusion: This study demonstrates the circulation of a previously unidentified species of the genus Anaplasma in Morocco which is genetically close to the agent causing canine anaplasmosis but whose main reservoir is thought to be Camelus dromedarius.
Bernard Faye - One of the best experts on this subject based on the ideXlab platform.
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Old World camels in a modern world - a balancing act between conservation and genetic improvement
Animal Genetics, 2019Co-Authors: Pamela Anna Burger, Elena Ciani, Bernard FayeAbstract:Old World camels have served humans in cross-continental caravans, transporting people and goods, connecting different cultures and providing milk, meat, wool and draught since their domestication around 3000-6000 years ago. In a world of modern transport and fast connectivity, these beasts of burden seem to be out-dated. However, a growing demand for sustainable milk and meat production, especially in countries affected by climate change and increasing desertification, brings dromedaries (Camelus dromedarius) and Bactrian camels (Camelus bactrianus) back onstage and into the focus of animal breeders and scientists. In this review on the molecular genetics of these economically important species we give an overview about the evolutionary history, domestication and dispersal of Old World camels, whereas highlighting the need for conservation of wild two-humped camels (Camelus ferus) as an evolutionarily unique and highly endangered species. We provide cutting-edge information on the current molecular resources and on-going sequencing projects. We cannot emphasise enough the importance of balancing the need for improving camel production traits with maintaining the genetic diversity in two domestic species with specific physiological adaptation to a desert environment.
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Sequence and polymorphism analysis of the camel (Camelus dromedarius) myostatin gene
Emirates Journal of Food and Agriculture, 2015Co-Authors: Stefania Muzzachi, Bernard Faye, Ahmad Oulmouden, Youcef Cherifi, Mohamed Habib Yahyaoui, Mohamed Zayed, Pamela Burger, Giovanni Michele Lacalandra, Elena CianiAbstract:Myostatin (MSTN), a negative regulator of skeletal muscle development in mammals, represents a key target for genetic investigations in meat-producing animals, with mutations responsible for increased skeletal-muscle mass currently described in several livestock species. Dromedary camels play a major economic role as suppliers of meat for human consumption across several countries. Notwithstanding, a comprehensive characterization of the sequence variability at the Camelus dromedarius MSTN locus was still lacking. Here we present the first extensive sequence and polymorphism analysis of the MSTN gene in the Camelus dromedarius species. Out of more than 3.6 kb of nucleotide sequence screened on 22 animals from 3 different Northern African regions, only 3 variant sites in the first intron were detected. The low observed diversity may reflect the evolutionary history of the species, likely developed as domesticates from a low variable wild ancestor population. Sequence identity among Camelus dromedarius and other Cetartiodactyla highlighted a tree topology consistent with previous reports of a closer relationship between Tylopoda and Suiformes. A close similarity between C. ferus and Camelus dromedarius was observed within Tylopoda. A markedly higher sequence identity between Camelus dromedarius and the other vertebrate species was observed at the MSTN locus compared to other genes, thus confirming it as a highly conserved target across mammals.
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Levels of PCDD/Fs and PCBs in Camel Milk (Camelus Bactrianus and Camelus Dromedarius) from Kazakhstan
Organohalogen compounds, 2011Co-Authors: Gaukhar Konuspayeva, Bernard Faye, Edwin De Pauw, Jean-françois FocantAbstract:a b s t r a c t To date, despite the fact it represents a very important part of the national dairy production, no data are available concerning the concentrations of polychlorinated dibenzo- p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and polychlorinated biphenyls (PCBs) in camel milk from the Republic of Kazakhstan. Selected PCDDs, PCDFs, and PCBs were measured in pools of milk from camels ( n = 15) located in various places of Kazakhstan (Almaty, Atyrau, Aralsk, Shymkent) and sampled at two different seasons for two different species (Camelus bactrianus and Camelus dromedarius). Non-dioxin-like (NDL)PCB concentrations (6.3 ± 2.7 ng g y1 fat, median 5.1 ng g y1 fat, range 0.6–17.4 ng g y1 fat) were far below the maximum value of 40 ng g y1 fat proposed by the EU. Dioxin-like (DL-)PCB concentrations (1.7 ± 0.7 ng g y1 fat, median 1.5 ng g y1 fat, range 0.3–4.2 ng g
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lactoferrin and immunoglobulin contents in camel s milk Camelus bactrianus Camelus dromedarius and hybrids from kazakhstan
Journal of Dairy Science, 2007Co-Authors: Gaukhar Konuspayeva, Bernard Faye, Gerard Loiseau, Didier LevieuxAbstract:Lactoferrin (Lf) and IgG were estimated in camel's milk from Kazakhstan, where 2 species of camels (Camelus bactrianus, Camelus dromedarius) and their hybrids cohabit. The concentrations of Lf and IgG were determined according to 3 variation factors: region (n = 4), season (n = 4), and species (n = 5; sample 4 was mixed milk and sample 5 was of unknown origin). The mean values in raw camel's milk were 0.229 ± 0.135 mg/mL for Lf concentration and 0.718 ± 0.330 mg/mL for IgG concentration. The seasonal effect was the only significant variation factor observed, with the highest values in the spring for Lf and in the winter for IgG. The Lf concentration varied in 1-wk postpartum milk from 1.422 to 0.586 mg/mL. The range in IgG concentration was wide and decreased from 132 to 4.75 mg/mL throughout the 7 d postpartum, with an important drop after parturition. In fermented milk, the lactoproteins are generally hydrolyzed. For milk samples from undefined species, discriminant analyses did not allow the origin of the species to be determined. A slight correlation between Lf and IgG concentrations was observed in raw milk. The values were slightly higher than those reported in cow's milk, but this difference was insufficient to attribute medicinal virtues to camel's milk.
O Alsagair - One of the best experts on this subject based on the ideXlab platform.
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the occurrence of thysanosoma actinioides diesing 1834 cestoda anoplocephalidae in a najdi camel in saudi arabia
Veterinary Parasitology, 2005Co-Authors: O. H. Omer, O AlsagairAbstract:The fringed tapeworm Thysanosoma actinioides was recovered from the liver of a Najdi camel (Camelus dromedarius) slaughtered in central Saudi Arabia. T. actinioides is recorded for the first time as a parasite of old world camels.
