The Experts below are selected from a list of 6 Experts worldwide ranked by ideXlab platform
Vincent L Vilker - One of the best experts on this subject based on the ideXlab platform.
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kinetic characterization of chiral biocatalysis of cycloarenes by the Camphor 5 Monooxygenase enzyme system
Journal of Molecular Catalysis B-enzymatic, 1999Co-Authors: David A Grayson, Vincent L VilkerAbstract:Abstract Redox enzyme mediated biocatalysis has the potential to regio- and stereo-specifically oxidize hydrocarbons producing valuable products with minimal by-product formation. In vitro reactions of the Camphor (cytochrome P -450) 5-Monooxygenase enzyme system with naphthalene-like substrates yield stereospecifically hydroxylated products from nonactivated hydrocarbons. Specifically, the enzyme system catalyzes the essentially stereospecific conversion of the cycloarene, tetralin (1,2,3,4-tetrahydronaphthalene) to ( R )-1-tetralol (( R )-(−)-1,2,3,4-tetrahydro-1-naphthol). It is shown that this reaction obeys Michaelis–Menten kinetics and that interactions between the enzyme subunits are not affected by the identity of the substrate. This subunit independence extends to the efficiency of NADH usage by the enzyme system—subunit ratios do not effect efficiency, but substrate identity does. Tetralin is converted at an efficiency of 13±3%, whereas ( R )-1-tetralol is converted at 7.8±0.7%. A model of this system based on Michaelis–Menten parameters for one subunit (Pdx: K M =10.2±2 μM) and both substrates (tetralin: K M =66±26 μM, ν max =0.11±0.04 s −1 , and ( R )-1-tetralol: K M =2800±1300 μM, ν max =0.83±0.22 s −1 ) is presented and used to predict the consumption and production of all substrates, products and cofactors.
David A Grayson - One of the best experts on this subject based on the ideXlab platform.
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kinetic characterization of chiral biocatalysis of cycloarenes by the Camphor 5 Monooxygenase enzyme system
Journal of Molecular Catalysis B-enzymatic, 1999Co-Authors: David A Grayson, Vincent L VilkerAbstract:Abstract Redox enzyme mediated biocatalysis has the potential to regio- and stereo-specifically oxidize hydrocarbons producing valuable products with minimal by-product formation. In vitro reactions of the Camphor (cytochrome P -450) 5-Monooxygenase enzyme system with naphthalene-like substrates yield stereospecifically hydroxylated products from nonactivated hydrocarbons. Specifically, the enzyme system catalyzes the essentially stereospecific conversion of the cycloarene, tetralin (1,2,3,4-tetrahydronaphthalene) to ( R )-1-tetralol (( R )-(−)-1,2,3,4-tetrahydro-1-naphthol). It is shown that this reaction obeys Michaelis–Menten kinetics and that interactions between the enzyme subunits are not affected by the identity of the substrate. This subunit independence extends to the efficiency of NADH usage by the enzyme system—subunit ratios do not effect efficiency, but substrate identity does. Tetralin is converted at an efficiency of 13±3%, whereas ( R )-1-tetralol is converted at 7.8±0.7%. A model of this system based on Michaelis–Menten parameters for one subunit (Pdx: K M =10.2±2 μM) and both substrates (tetralin: K M =66±26 μM, ν max =0.11±0.04 s −1 , and ( R )-1-tetralol: K M =2800±1300 μM, ν max =0.83±0.22 s −1 ) is presented and used to predict the consumption and production of all substrates, products and cofactors.
Hue Sun Chan - One of the best experts on this subject based on the ideXlab platform.
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Examples of proteins with putative high bi-stability.
2012Co-Authors: Tobias Sikosek, Erich Bornberg-bauer, Hue Sun ChanAbstract:Proteins are ordered by increasing stability difference (decreasing bi-stability) and they may be classified into the following functional categories: metabolism (Purine-nucleoside phosphorylase, Camphor 5-Monooxygenase, Glycogen phosphorylase, Adenylate kinase, Hydrolase, Cytochrome P450 119, Light-harvesting protein B-875 beta chain), transcriptional regulation (HTH-type transcriptional regulator qacR, Regulatory protein rop), epigenetic regulation (Histone-lysine N-methyltransferase, Chromobox protein homolog 1), signalling (Synapsin-2, Protein enhancer of sevenless 2B, Baculoviral IAP repeat-containing protein 4, Antithrombin-III, Lymphotactin), translation (50S ribosomal protein L11), cytoskeleton (Talin-1, Synapsin-2), and host-pathogen interaction/immune system (Ig epsilon chain C region, Nitrophorin-4, Baculoviral IAP repeat-containing protein 4, Lymphotactin, Capsid protein G8P).aCATH [64] structural domain identifiers.bRoot Mean Square Deviation of backbone atoms; in units of Å.cRosetta standard free energy score/domain length.
Tobias Sikosek - One of the best experts on this subject based on the ideXlab platform.
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Examples of proteins with putative high bi-stability.
2012Co-Authors: Tobias Sikosek, Erich Bornberg-bauer, Hue Sun ChanAbstract:Proteins are ordered by increasing stability difference (decreasing bi-stability) and they may be classified into the following functional categories: metabolism (Purine-nucleoside phosphorylase, Camphor 5-Monooxygenase, Glycogen phosphorylase, Adenylate kinase, Hydrolase, Cytochrome P450 119, Light-harvesting protein B-875 beta chain), transcriptional regulation (HTH-type transcriptional regulator qacR, Regulatory protein rop), epigenetic regulation (Histone-lysine N-methyltransferase, Chromobox protein homolog 1), signalling (Synapsin-2, Protein enhancer of sevenless 2B, Baculoviral IAP repeat-containing protein 4, Antithrombin-III, Lymphotactin), translation (50S ribosomal protein L11), cytoskeleton (Talin-1, Synapsin-2), and host-pathogen interaction/immune system (Ig epsilon chain C region, Nitrophorin-4, Baculoviral IAP repeat-containing protein 4, Lymphotactin, Capsid protein G8P).aCATH [64] structural domain identifiers.bRoot Mean Square Deviation of backbone atoms; in units of Å.cRosetta standard free energy score/domain length.
Erich Bornberg-bauer - One of the best experts on this subject based on the ideXlab platform.
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Examples of proteins with putative high bi-stability.
2012Co-Authors: Tobias Sikosek, Erich Bornberg-bauer, Hue Sun ChanAbstract:Proteins are ordered by increasing stability difference (decreasing bi-stability) and they may be classified into the following functional categories: metabolism (Purine-nucleoside phosphorylase, Camphor 5-Monooxygenase, Glycogen phosphorylase, Adenylate kinase, Hydrolase, Cytochrome P450 119, Light-harvesting protein B-875 beta chain), transcriptional regulation (HTH-type transcriptional regulator qacR, Regulatory protein rop), epigenetic regulation (Histone-lysine N-methyltransferase, Chromobox protein homolog 1), signalling (Synapsin-2, Protein enhancer of sevenless 2B, Baculoviral IAP repeat-containing protein 4, Antithrombin-III, Lymphotactin), translation (50S ribosomal protein L11), cytoskeleton (Talin-1, Synapsin-2), and host-pathogen interaction/immune system (Ig epsilon chain C region, Nitrophorin-4, Baculoviral IAP repeat-containing protein 4, Lymphotactin, Capsid protein G8P).aCATH [64] structural domain identifiers.bRoot Mean Square Deviation of backbone atoms; in units of Å.cRosetta standard free energy score/domain length.