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Elerson Gaettijardim - One of the best experts on this subject based on the ideXlab platform.
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subgingival microbiota from cebus apella capuchin monkey with different periodontal conditions
Anaerobe, 2012Co-Authors: Lira Marcela Monti, Francisco Isaak Nicolas Ciesielski, Ana Claudia Okamoto, Christiane Marie Schweitzer, Elerson Gaettijardim, Mario Julio AvilacamposAbstract:Abstract This present study evaluated the subgingival microbiota of the Cebus apella with different periodontal conditions kept by the Tufted Capuchin Monkey Procreation Center (Sao PauloState University – UNESP) or free-ranging monkeys. For this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. The primates were submitted to periodontal evaluation and biofilm samples were transferred to VMGA III transport medium and ultrapure water. The microbiota was cultivated in selective and non-selective culture media and microbial DNA was extracted and the presence of periodontal pathogens was evaluated using PCR and real-time PCR. The actinomycetes, fusobacteria, Campylobacter Rectus , Eikenella corrodens , black-pigmented Gram-negative anaerobic rods, Tannerella forsythia , staphylococci and streptococci represent the predominantly detected microorganisms. Aggregatibacter actinomycetemcomitans , Dialister pneumosintes and Prevotella nigrescens were rarely observed, whereas Treponema denticola was not found. Populations of C. Rectus , E. corrodens, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, T. forsythia and the total microbial load were significantly higher in animals with bone loss and, in smaller extension, in animals with gingival bleeding.
Steven Offenbacher - One of the best experts on this subject based on the ideXlab platform.
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toll like receptor 4 mediates intrauterine growth restriction after systemic Campylobacter Rectus infection in mice
Molecular Oral Microbiology, 2012Co-Authors: Roger M Arce, Silvana P Barros, K M Caron, Steven OffenbacherAbstract:Summary Campylobacter Rectus is associated with fetal exposure and low birthweight in humans. C. Rectus also invades placental tissues and induces fetal intrauterine growth restriction (IUGR) in mice, along with overexpression of Toll-like receptors (TLR4), suggesting that TLR4 may mediate placental immunity and IUGR in mice. To test this hypothesis we examined the effect of in vitro TLR4 neutralization on trophoblastic proinflammatory activity and studied the IUGR phenotype in a congenic TLR4-mutant mouse strain after in vivo C. Rectus infection. Human trophoblasts were pretreated with TLR4 neutralizing antibodies and infected with C. Rectus; proinflammatory cytokine production was assessed by cytokine multiplex assays. Neutralizing TLR4 antibodies significantly impaired the production of proinflammatory cytokines in trophoblastic cells after infection in a dose-dependent manner. We used a subcutaneous chamber model to provide a C. Rectus challenge in BALB/cAnPt (TLR4Lps-d) and wild-type (WT) females. Females were mated with WT or TLR4Lps-d males once/week; pregnant mice were infected at (E)7.5 and sacrificed at (E)16.5 to establish IUGR phenotypes. Maternal C. Rectus infection significantly decreased fetal weight/length in infected WT when compared with sham WT controls (P 0.05). Furthermore, heterozygous TLR4Lps-d+/− fetuses showed IUGR phenotype rescue. We conclude that TLR4 is an important mediator of trophoblastic proinflammatory responses and TLR4-deficient fetuses do not develop IUGR phenotypes after C. Rectus infection, suggesting that placental cytokine activation is likely to be mediated by TLR4 during low birthweight/preterm birth pathogenesis.
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effects of maternal Campylobacter Rectus infection on murine placenta fetal and neonatal survival and brain development
Journal of Periodontology, 2005Co-Authors: Steven Offenbacher, Estelle L Riche, Silvana P Barros, Yiorgos A Bobetsis, Dongming Lin, James D BeckAbstract:Background: Maternal periodontal infection has been associated with increased risk of prematurity and low birthweight. Infection and inflammatory pathways that mediate prematurity have also been implicated in neonatal developmental impairments. The objective of this study was to determine whether maternal Campylobacter Rectus infection that induces fetal growth restriction in a mouse model also compromises neonatal pup survival, growth, and neurodevelopment.Methods: Timed pregnant mice were challenged with C. Rectus on gestation day 7.5. One group of animals was sacrificed on embryonic day 16.5 for placental histology and measurement of fetal brain mRNA expression of tumor necrosis factor (TNF)-α and interferon (IFN)-γ. Another group of animals was allowed to deliver to follow pup survival, growth, and brain structure at day 9.Results: C. Rectus challenge resulted in abnormal placental architecture with inflammation and a 2.8-fold increase in fetal brain expression of IFN-γ (P = 0.04). Pup birthweight was...
Mario Julio Avilacampos - One of the best experts on this subject based on the ideXlab platform.
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subgingival microbiota from cebus apella capuchin monkey with different periodontal conditions
Anaerobe, 2012Co-Authors: Lira Marcela Monti, Francisco Isaak Nicolas Ciesielski, Ana Claudia Okamoto, Christiane Marie Schweitzer, Elerson Gaettijardim, Mario Julio AvilacamposAbstract:Abstract This present study evaluated the subgingival microbiota of the Cebus apella with different periodontal conditions kept by the Tufted Capuchin Monkey Procreation Center (Sao PauloState University – UNESP) or free-ranging monkeys. For this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. The primates were submitted to periodontal evaluation and biofilm samples were transferred to VMGA III transport medium and ultrapure water. The microbiota was cultivated in selective and non-selective culture media and microbial DNA was extracted and the presence of periodontal pathogens was evaluated using PCR and real-time PCR. The actinomycetes, fusobacteria, Campylobacter Rectus , Eikenella corrodens , black-pigmented Gram-negative anaerobic rods, Tannerella forsythia , staphylococci and streptococci represent the predominantly detected microorganisms. Aggregatibacter actinomycetemcomitans , Dialister pneumosintes and Prevotella nigrescens were rarely observed, whereas Treponema denticola was not found. Populations of C. Rectus , E. corrodens, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, T. forsythia and the total microbial load were significantly higher in animals with bone loss and, in smaller extension, in animals with gingival bleeding.
Silvana P Barros - One of the best experts on this subject based on the ideXlab platform.
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toll like receptor 4 mediates intrauterine growth restriction after systemic Campylobacter Rectus infection in mice
Molecular Oral Microbiology, 2012Co-Authors: Roger M Arce, Silvana P Barros, K M Caron, Steven OffenbacherAbstract:Summary Campylobacter Rectus is associated with fetal exposure and low birthweight in humans. C. Rectus also invades placental tissues and induces fetal intrauterine growth restriction (IUGR) in mice, along with overexpression of Toll-like receptors (TLR4), suggesting that TLR4 may mediate placental immunity and IUGR in mice. To test this hypothesis we examined the effect of in vitro TLR4 neutralization on trophoblastic proinflammatory activity and studied the IUGR phenotype in a congenic TLR4-mutant mouse strain after in vivo C. Rectus infection. Human trophoblasts were pretreated with TLR4 neutralizing antibodies and infected with C. Rectus; proinflammatory cytokine production was assessed by cytokine multiplex assays. Neutralizing TLR4 antibodies significantly impaired the production of proinflammatory cytokines in trophoblastic cells after infection in a dose-dependent manner. We used a subcutaneous chamber model to provide a C. Rectus challenge in BALB/cAnPt (TLR4Lps-d) and wild-type (WT) females. Females were mated with WT or TLR4Lps-d males once/week; pregnant mice were infected at (E)7.5 and sacrificed at (E)16.5 to establish IUGR phenotypes. Maternal C. Rectus infection significantly decreased fetal weight/length in infected WT when compared with sham WT controls (P 0.05). Furthermore, heterozygous TLR4Lps-d+/− fetuses showed IUGR phenotype rescue. We conclude that TLR4 is an important mediator of trophoblastic proinflammatory responses and TLR4-deficient fetuses do not develop IUGR phenotypes after C. Rectus infection, suggesting that placental cytokine activation is likely to be mediated by TLR4 during low birthweight/preterm birth pathogenesis.
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effects of maternal Campylobacter Rectus infection on murine placenta fetal and neonatal survival and brain development
Journal of Periodontology, 2005Co-Authors: Steven Offenbacher, Estelle L Riche, Silvana P Barros, Yiorgos A Bobetsis, Dongming Lin, James D BeckAbstract:Background: Maternal periodontal infection has been associated with increased risk of prematurity and low birthweight. Infection and inflammatory pathways that mediate prematurity have also been implicated in neonatal developmental impairments. The objective of this study was to determine whether maternal Campylobacter Rectus infection that induces fetal growth restriction in a mouse model also compromises neonatal pup survival, growth, and neurodevelopment.Methods: Timed pregnant mice were challenged with C. Rectus on gestation day 7.5. One group of animals was sacrificed on embryonic day 16.5 for placental histology and measurement of fetal brain mRNA expression of tumor necrosis factor (TNF)-α and interferon (IFN)-γ. Another group of animals was allowed to deliver to follow pup survival, growth, and brain structure at day 9.Results: C. Rectus challenge resulted in abnormal placental architecture with inflammation and a 2.8-fold increase in fetal brain expression of IFN-γ (P = 0.04). Pup birthweight was...
Panos N Papapanou - One of the best experts on this subject based on the ideXlab platform.
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Serum IgG Antibody Levels to Periodontal Microbiota Are Associated with Incident Alzheimer Disease
2014Co-Authors: James M. Noble, Nikolaos Scarmeas, Romanita S. Celenti, Mitchell S. V. Elkind, Clinton B. Wright, Nicole Schupf, Panos N PapapanouAbstract:BackgroundPeriodontitis and Alzheimer disease (AD) are associated with systemic inflammation. This research studied serum IgG to periodontal microbiota as possible predictors of incident AD.MethodsUsing a case-cohort study design, 219 subjects (110 incident AD cases and 109 controls without incident cognitive impairment at last follow-up), matched on race-ethnicity, were drawn from the Washington Heights-Inwood Columbia Aging Project (WHICAP), a cohort of longitudinally followed northern Manhattan residents aged >65 years. Mean follow-up was five years (SD 2.6). In baseline sera, serum IgG levels were determined for bacteria known to be positively or negatively associated with periodontitis (Porphyromonas gingivalis, Tannerella forsythia, Actinobacillus actinomycetemcomitans Y4, Treponema denticola, Campylobacter Rectus, Eubacterium nodatum, and Actinomyces naeslundii genospecies-2). In all analyses, we used antibody threshold levels shown to correlate with presence of moderate-severe periodontitis.ResultsMean age was 72 years (SD 6.9) for controls, and 79 years (SD 4.6) for cases (p640 ng/ml, present in 10% of subjects) was associated with increased risk of AD (HR = 2.0, 95%CI: 1.1–3.8). This association was stronger after adjusting for other significant titers (HR = 3.1, 95%CI: 1.5–6.4). In this model, high anti-E. nodatum IgG (>1755 ng/ml; 19% of subjects) was associated with lower risk of AD (HR = 0.5, 95%CI: 0.2–0.9).ConclusionsSerum IgG levels to common periodontal microbiota are associated with risk for developing incident AD.
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bleeding on probing differentially relates to bacterial profiles the oral infections and vascular disease epidemiology study
Journal of Clinical Periodontology, 2008Co-Authors: Ryan T Demmer, Panos N Papapanou, Moise Desvarieux, David R JacobsAbstract:Aim: Various bacterial species are differentially prevalent in periodontal health, gingivitis or periodontitis. We tested the independent associations between three bacterial groupings and gingival inflammation in an epidemiological study. Material and Methods: In 706 Oral Infections and Vascular Disease Epidemiology Study (INVEST) participants 55 years, bleeding on probing (BoP), pocket depth (PD) and subgingival plaque samples (n=4866) were assessed in eight sites per mouth. Eleven bacterial species were quantitatively assayed and grouped as follows: (i) aetiologic burden (EB, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia); (ii) putative burden (PB, Campylobacter Rectus, Eikenella corrodens, Fusobacterium nucleatum, Micromonas micros, Prevotella intermedia); (iii) health-associated burden (HAB, Actinomyces naeslundii, Veillonella parvula). Results: After mutual adjustment for EB, PB and HAB, the BoP prevalence increased by 45% ( p<0.0001) across increasing quartiles of EB while BoP decreased by 13% ( p<0.0001) across increasing quartiles of HAB. Mean PD increased 0.8 mm and decreased 0.3 mm from the first to fourth quartiles of EB (p<0.0001) and HAB ( p<0.0001), respectively. Among 1214 plaque samples with fourth quartile EB, 60% were collected from sites with PD 3 mm. Conclusion: Bacterial species believed to be aetiologically related to periodontitis were associated with BoP in sites with minimal PD and/or attachment level (AL). Species presumed to be associated with periodontal health demonstrated inverse associations with BoP.
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periodontal infections and pre term birth early findings from a cohort of young minority women in new york
European Journal of Oral Sciences, 2001Co-Authors: Dennis Mitchelllewis, Steven P Engebretson, Judy Y C Chen, Ira B Lamster, Panos N PapapanouAbstract:The aim of this report is to provide early data from an ongoing study examining (i) the relationship between periodontal infections and pre-term low birth weight (PLBW) in a cohort of young, minority, pregnant and post-partum women; and (ii) the effect of periodontal interventions on pregnancy outcome. During the first 2 yr of the study, 213 women were enrolled and examined clinically for dental plaque, calculus, bleeding on probing, and probing depth. Birth outcome data were available for 164 women, including one group (n = 74) subjected to oral prophylaxis during pregnancy, and a second group (n=90) who received no prenatal periodontal treatment. Subgingival plaque samples were available from 145 subjects (4 samples/subject) and were analyzed by checkerboard DNA hybridization with respect to 12 bacterial species. The prevalence of PLBW was 16.5% (27 cases) in this cohort. No differences in clinical periodontal status were observed between PLBW cases and women with normal birth outcome. However, PLBW mothers had significantly higher levels of Bacteroides forsythus and Campylobacter Rectus, and consistently elevated counts for the other species examined. PLBW occurred in 18.9% of the women who did not receive periodontal intervention (17 cases), and in 13.5% (10 cases) of those who received such therapy.
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checkerboard versus culture a comparison between two methods for identification of subgingival microbiota
European Journal of Oral Sciences, 1997Co-Authors: Panos N Papapanou, Phoebus N Madianos, Gunnar Dahlen, Jens SandrosAbstract:The present study compared the "checkerboard" DNA-DNA hybridization methodology with culture techniques for the analysis of the composition of the subgingival microbiota. 70 subjects, presenting with a variety of periodontal conditions, contributed with a total of 283 subgingival plaque samples analyzed with respect to the following species: Porphyromonas gingivalis, Prevotella intermedia/Prevotella nigrescens, Fusobacterium nucleatum, Campylobacter Rectus, Eikenella corrodens, Bacteroides forsythus, Actinobacillus actinomycetemcomitans, Streptococcus sanguis and Streptococcus mutans. Species identification and quantification was performed by (i) the checkerboard method, using whole genomic, digoxigenin labeled DNA probes; and (ii) culture, including non-selective and selective media in combination with routine biochemical testing using commercial test panels. We found that the checkerboard technology resulted in higher prevalence figures for half of the species tested when compared to culture data. If the latter were used as the reference, checkerboard detection sensitivities ranged from 0.17 to 0.86, specificities from 0.17 to 1.0, and diagnostic accuracies from 0.51 to 0.81, depending on bacterial species. The use of the checkerboard data as the reference resulted in detection sensitivities for the culture procedures between 0.24 and 1.0 and specificities between 0.21 and 0.87. The checkerboard methodology resulted in statistically significant higher bacterial counts for the majority of the species. It was further observed that, for most species, the higher the total number colony-forming units in the sample, the higher the discrepancy between the results obtained by the two techniques.