The Experts below are selected from a list of 228 Experts worldwide ranked by ideXlab platform
H. W. Doelle - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous fructose and ethanol production from sucrose, usingZymomonas mobilis 2864 co-immobilised with invertase
Biotechnology Letters, 1994Co-Authors: Lisa A. Kirk, H. W. DoelleAbstract:Fructokinase negative Zymomonas mobilis UQM 2864, was co-immobilised with invertase in alginate and incubated on sucrose-based media in batch and fedbatch culture. The highest fructose concentration achieved was 138 g/l using fed-batch cultivation with sugar-Cane Syrup-simultaneously producing 79.9 g/l or 10.1% (v/v) ethanol in less than 24 hours. The ethanol and fructose yields were 95 and 84% respectively. Co-immobilisation resulted in faster fermentation times, particularly for the batch fermentations, and complete utilisation of substrate.
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Simultaneous fructose and ethanol production from sucrose, using Zymomonas mobilis 2864 co-immobilised with invertase
Biotechnology Letters, 1994Co-Authors: Lisa A. Kirk, H. W. DoelleAbstract:Fructokinase negativeZymomonas mobilis UQM 2864, was co-immobilised with invertase in alginate and incubated on sucrose-based media in batch and fedbatch culture. The highest fructose concentration achieved was 138 g/l using fed-batch cultivation with sugar-Cane Syrup-simultaneously producing 79.9 g/l or 10.1% (v/v) ethanol in less than 24 hours. The ethanol and fructose yields were 95 and 84% respectively. Co-immobilisation resulted in faster fermentation times, particularly for the batch fermentations, and complete utilisation of substrate.
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Rapid ethanol production from sucrose without by-product formation
Biotechnology Letters, 1993Co-Authors: Lisa A. Kirk, H. W. DoelleAbstract:Non-sorbitol-producing Zymomonas mobilis ACM 3963 was developed from Z. mobilis UQM 2716. This strain was co-immobilised with invertase in alginate and incubated on sucrose-based media. This combination allowed theoretical yields of ethanol to be produced from 100 and 150 g/l sucrose, using both semi-defined media and sugar-Cane Syrup. No sorbitol or fructo-oligosaccharides were formed in either fermentation. Increased biomass concentrations immobilised in alginate reduced the batch fermentation times of 100 and 150 g/l sucrose by 50–70%, to 3 and 5 hours respectively. This strain also improved the efficiency of the fed-batch fermentation of sucrose.
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Scale-up of ethanol production from sugarCane usingZymomonas mobilis
Biotechnology Letters, 1991Co-Authors: H. W. Doelle, L. D. Kennedy, M. B. DoelleAbstract:The Zymomonas fermentation for industrial ethanol production has been successfully scaled up. Pilot plant experiments at 100 and 1,000 litre fermentation capacity gave 91–95% conversion efficiencies and up to 10% (v/v) ethanol yields within 17–20 hours using sugar Cane Syrup, A-, B-, and C-molasses with the addition of sucrose or Syrup to a final 15% total sugar concentration.
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Scale-up of ethanol production from sugarCane using Zymomonas mobilis
Biotechnology Letters, 1991Co-Authors: H. W. Doelle, L. D. Kennedy, M. B. DoelleAbstract:TheZymomonas fermentation for industrial ethanol production has been successfully scaled up. Pilot plant experiments at 100 and 1,000 litre fermentation capacity gave 91–95% conversion efficiencies and up to 10% (v/v) ethanol yields within 17–20 hours using sugar Cane Syrup, A-, B-, and C-molasses with the addition of sucrose or Syrup to a final 15% total sugar concentration.
Maria Lurdes Felsner - One of the best experts on this subject based on the ideXlab platform.
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Development an in House Validation for 5-Hydroxy-2-methyl-furfuraldehyde (HMF) Analysis in Fermented Beverages Produced from Honey, Cane Syrup and Corn Syrup by HPLC-UV
Orbital: The Electronic Journal of Chemistry, 2018Co-Authors: João Paulo Niemes, Christiane Schineider Machado Kolc, Yohandra Reyes Torres, Maria Lurdes Felsner, Marcos Roberto Da RosaAbstract:A chromatographic direct method using HPLC/UV for quantification of 5-hydroxy-2-methyfurfuraldehyde (HMF) content in different fermented beverages (mead, Cane Syrup fermented and corn Syrup fermented) was developed and in house validated using sophisticated statistical tools for the first time in this work. HMF separation was executed with isocratic elution of a mobile phase comprising water (with 0.5% formic acid) and acetonitrile (90:10, v v -1 ), at 30 o C, flow rate of 1.0 mL min -1 , injection volume of 5.0 µL and detection at 285 nm. Validation study demonstrated that the developed method has good performance, presenting low limits (LOD and LOQ of 0.16 and 0.53 mg L -1 , respectively), good accuracy (recovery rates between 82.3 and 95.9%) and precision (RSD values between 3.87 and 8.84% and Horrat values between 0.43 and 0.76). Adequate selectivity and linearity estimates were also observed (R 2 > 99.5%). Fermented beverages from honey, Cane Syrup and corn Syrup presented HMF contents lower than starting foods used in fermentation. Besides this, results demonstrated that fermentation conditions are a key parameter for obtaining fermented beverages with low levels of this contaminant and that the fermentation can be a strategy for mitigation of HMF in foods such honey, Cane Syrup and corn Syrup. DOI: http://dx.doi.org/10.17807/orbital.v10i3.1079
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Isothermal Thermogravimetry and Factorial Design Applied to Development and Validation of an Oven Drying Method for Cane Syrups
Orbital: The Electronic Journal of Chemistry, 2018Co-Authors: Giseli Ducat, Maria Lurdes Felsner, Sueli Pércio Quináia, Pedro Ramos Da Costa NetoAbstract:A new and green loss drying method in conventional oven for moisture analysis in Cane Syrup was developed and in house validated at the first time in this work. According factorial design results, the best conditions of drying in oven were the use of fiber glass paper discs, drying temperature of 70 °C and drying time of 90 min. In-house validation study demonstrated that the developed drying method presents performance comparable to that of Karl Fischer titration in terms of precision and accuracy as verified by results of F-test of variances comparison and of paired t-test. This loss drying method is advantageous because it uses low cost instrumentation, is environmentally friendly since it does not use toxic solvents and it could be applied in quality control laboratories, by supervisory bodies or by small industries. DOI: http://dx.doi.org/10.17807/orbital.v10i3.1114
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In house validation from direct determination of 5-hydroxymethyl-2-furfural (HMF) in Brazilian corn and Cane Syrups samples by HPLC-UV.
Food chemistry, 2015Co-Authors: Jucimara Kulek De Andrade, Yohandra Reyes Torres, Marcos Roberto Da Rosa, Emy Komatsu, Hélène Perreault, Maria Lurdes FelsnerAbstract:In this work the development and in house validation of the HMF direct determination in corn and Cane Syrups by HPLC-UV was carried out for the first time. The separation was done with isocratic elution of a mobile phase comprising water (with 0.5% formic acid) and acetonitrile (90:10, v/v) on Phenomenex C18 column (5.0 μm, 4.6 × 150 mm), at 30 °C, flow rate of 0.8 mL min(-1) and detection at 285 nm. The validated method showed excellent performance with low limits (LOD and LOQ of 0.09 and 0.26 mg L(-1), respectively), good accuracy (recovery rates between 100% and 104%) and precision (RSD's for repeatability and intermediate precision between 0.57% and 6.43%). Good selectivity and linearity were also observed. HMF contents in both foods were very high (406.6-2121.3 mg kg(-1) for corn Syrup and 109.2-893.1 mg kg(-1) for Cane Syrup), which arouses concern about food safety of these products.
Maria Antonia Pedrine Colabone Celligoi - One of the best experts on this subject based on the ideXlab platform.
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study of levan production by zymomonas mobilis using regional low cost carbohydrate sources
Biochemical Engineering Journal, 2007Co-Authors: Marcos Roberto Gois De Oliveira, Rui Sergio Dos Santos Ferreira Da Silva, Joao Batista Buzato, Maria Antonia Pedrine Colabone CelligoiAbstract:The use of alternative regional low-cost substrates has become very interesting because in addition to the ease of acquisition it presents a relatively low cost. In this study using statistical instruments, the exopolysaccharide levan production by the Zymomonas mobilis microorganism was analyzed varying the carbon source (commercial sucrose, molasses and sugar Cane Syrups) and the fermentation medium constituents. There was a decrease in levan production in the molasses medium (2.533 g L −1 ) when compared to the commercial sucrose (21.685 g L −1 ) and Syrup (15.456 g L −1 ) media. Yeast extract and KH2PO4 were significant in the commercial sucrose medium for levan production and in the Syrup medium the yeast extract and MgSO4 were significant. Although sugar Cane Syrup up produced about 28.724% less levan compared to commercial sucrose, biomass production in the Syrup was 2.76 times greater than in commercial sucrose (0.857 and 2.366 g L −1 ) that could justify joint levan and Z. mobilis ATCC 31821 biomass production. Studies are needed on the use of alternative substrates and complexes in biotechnology to assess the composition of these media, avoiding unnecessary supplementation with vitamins and minerals salts, when using the formulations of the mediums defined for the complex mediums. © 2007 Elsevier B.V. All rights reserved.
C. Laluce - One of the best experts on this subject based on the ideXlab platform.
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Enrichment of a continuous culture of Saccharomyces cerevisiae with the yeast Issatchenkia orientalis in the production of ethanol at increasing temperatures
Journal of Industrial Microbiology & Biotechnology, 2011Co-Authors: J. C. M. Gallardo, C. S. Souza, R. M. B. Cicarelli, K. F. Oliveira, M. R. Morais, C. LaluceAbstract:A fermentation system was continuously fed with sugar-Cane Syrup and operated with recycling of Saccharomyces cerevisiae cells at temperatures varying from 30 to 47°C. The aim of the present work was to obtain and study the colonies of isolates showing elongated cells of yeasts which were sporadically observed at the end of this continuous process. Based on a sequence of assays involving methods of classical taxonomy and RAPD-PCR, two groups of isolates showing characteristics of non- Saccharomyces yeasts were identified in the yeast population where S. cerevisiae was the dominant yeast. The largest group of non- Saccharomyces yeasts, resulting from a slow proliferation over the 2 months, reached a final level of 29.6% at the end of the process. RAPD-PCR profiles obtained for the isolates of this dominant non- Saccharomyces yeast indicated that they were isolates of Issatchenkia orientalis . Pichia membranifaciens was the only species of non- Saccharomyces yeast detected together with I. orientalis but at a very low frequency. The optimum temperature for ethanol formation shown by the isolate 195B of I. orientalis was 42°C. This strain also showed a faster ethanol formation and biomass accumulation than the thermotolerant strain of S. cerevisiae used as the starter of this fermentation process. Some isolates of I. orientalis were also able to grow better at 40°C than at 30°C on plates containing glycerol as carbon source. Yeasts able to grow and produce ethanol at high temperatures can extend the fermentation process beyond the temperature limits tolerated by S. cerevisiae.
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Thermotolerance behavior in sugar Cane Syrup fermentations of wild type yeast strains selected under pressures of temperature, high sugar and added ethanol
Biotechnology Letters, 1993Co-Authors: C. Laluce, C. L. Abud, W. Greenhalf, Peres M. F. SanchesAbstract:The selected yeast strains were examined for their ability to grow, to retain cell viability and to ferment diluted sugar Cane juice (15 % total sugar, w/v) to ethanol at 40°C. The degree of agitation (aeration) affects the thermotolerance while the method used for isolation of the strains appears to have no significant effect. The yeast isolated are aerobically fermentative with increased levels of fermentation and growth resulting from agitation (aeration), the exact level of these increases being dependent on the strain used.
Marie Just Mikkelsen - One of the best experts on this subject based on the ideXlab platform.
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Anaerobic, Thermophilic Bacterium
2016Co-Authors: Pretreated Lignocellulosic Biomasses, Rasmus Lund Andersen, Karen Møller Jensen, Marie Just Mikkelsen, Estibio Aps BallerupAbstract:Lignocellosic ethanol production is now at a stage where commercial or semi-commercial plants are coming online and, provided cost effective production can be achieved, lignocel-lulosic ethanol will become an important part of the world bio economy. However, chal-lenges are still to be overcome throughout the process and particularly for the fermentation of the complex sugar mixtures resulting from the hydrolysis of hemicellulose. Here we describe the continuous fermentation of glucose, xylose and arabinose from non-detoxified pretreated wheat straw, birch, corn cob, sugar Cane bagasse, cardboard, mixed bio waste, oil palm empty fruit bunch and frond, sugar Cane Syrup and sugar Cane molasses using the anaerobic, thermophilic bacterium Thermoanaerobacter Pentocrobe 411. All fermentations resulted in close to maximum theoretical ethanol yields of 0.47–0.49 g/g (based on glucose, xylose, and arabinose), volumetric ethanol productivities of 1.2–2.7 g/L/h and a total sugar conversion of 90–99 % including glucose, xylose and arabinose. The results solidify the potential of Thermoanaerobacter strains as candidates for lignocellulose bioconversion
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Continuous Ethanol Fermentation of Pretreated Lignocellulosic Biomasses, Waste Biomasses, Molasses and Syrup Using the Anaerobic, Thermophilic Bacterium Thermoanaerobacter italicus Pentocrobe 411
PloS one, 2015Co-Authors: Rasmus Lund Andersen, Karen Møller Jensen, Marie Just MikkelsenAbstract:Lignocellosic ethanol production is now at a stage where commercial or semi-commercial plants are coming online and, provided cost effective production can be achieved, lignocellulosic ethanol will become an important part of the world bio economy. However, challenges are still to be overcome throughout the process and particularly for the fermentation of the complex sugar mixtures resulting from the hydrolysis of hemicellulose. Here we describe the continuous fermentation of glucose, xylose and arabinose from non-detoxified pretreated wheat straw, birch, corn cob, sugar Cane bagasse, cardboard, mixed bio waste, oil palm empty fruit bunch and frond, sugar Cane Syrup and sugar Cane molasses using the anaerobic, thermophilic bacterium Thermoanaerobacter Pentocrobe 411. All fermentations resulted in close to maximum theoretical ethanol yields of 0.47–0.49 g/g (based on glucose, xylose, and arabinose), volumetric ethanol productivities of 1.2–2.7 g/L/h and a total sugar conversion of 90–99% including glucose, xylose and arabinose. The results solidify the potential of Thermoanaerobacter strains as candidates for lignocellulose bioconversion.
