The Experts below are selected from a list of 921 Experts worldwide ranked by ideXlab platform
David B Grandy - One of the best experts on this subject based on the ideXlab platform.
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thermal probe based analytical microscopy thermal analysis and photothermal fourier transform infrared microspectroscopy together with thermally assisted nanosampling coupled with Capillary electrophoresis
Analytical Chemistry, 2009Co-Authors: Xuan Dai, Jonathan Moffat, Andrew G Mayes, Mike Reading, Duncan Q M Craig, P S Belton, David B GrandyAbstract:In this study, we have demonstrated that a scanning probe microscope (SPM) can be used for thermally assisted nanosampling (TAN) with subsequent analysis by Capillary electrophoresis (CE). Localized thermomechanical analysis (L-TMA) and photothermal Fourier-transform infrared (PT-FTIR) microspectroscopy can also be employed using the same probe, thus illustrating how a single instrument can carry out a number of different complementary analytical measurements. Benzoic acid and 4-hydroxybenzoic acid were manipulated with a heated Wollaston wire probe and successfully deposited onto the surface of a piece of CE Capillary Tubing. The deposited samples were then separated with CE. L-TMA and PT-FTIR were also used to characterize these materials. We have also demonstrated how a nanosample of a nonparticulate material can be taken and then deposited onto the surface of an inert matrix. TAN of a nonparticulate material was explored using polyethylene as the analyte and fluorene as the matrix. These examples show...
Xuan Dai - One of the best experts on this subject based on the ideXlab platform.
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thermal probe based analytical microscopy thermal analysis and photothermal fourier transform infrared microspectroscopy together with thermally assisted nanosampling coupled with Capillary electrophoresis
Analytical Chemistry, 2009Co-Authors: Xuan Dai, Jonathan Moffat, Andrew G Mayes, Mike Reading, Duncan Q M Craig, P S Belton, David B GrandyAbstract:In this study, we have demonstrated that a scanning probe microscope (SPM) can be used for thermally assisted nanosampling (TAN) with subsequent analysis by Capillary electrophoresis (CE). Localized thermomechanical analysis (L-TMA) and photothermal Fourier-transform infrared (PT-FTIR) microspectroscopy can also be employed using the same probe, thus illustrating how a single instrument can carry out a number of different complementary analytical measurements. Benzoic acid and 4-hydroxybenzoic acid were manipulated with a heated Wollaston wire probe and successfully deposited onto the surface of a piece of CE Capillary Tubing. The deposited samples were then separated with CE. L-TMA and PT-FTIR were also used to characterize these materials. We have also demonstrated how a nanosample of a nonparticulate material can be taken and then deposited onto the surface of an inert matrix. TAN of a nonparticulate material was explored using polyethylene as the analyte and fluorene as the matrix. These examples show...
Stephen G. Weber - One of the best experts on this subject based on the ideXlab platform.
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In vivo monitoring of serotonin in the striatum of freely moving rats with one minute temporal resolution by online microdialysis-Capillary high-performance liquid chromatography at elevated temperature and pressure
Analytical Chemistry, 2013Co-Authors: Jing Zhang, Andrea Jaquins-gerstl, Kathryn M. Nesbitt, Adrian C Michael, Sarah C. Rutan, Stephen G. WeberAbstract:Online monitoring of serotonin in striatal dialysate from freely moving rats was carried out for more than 16 h at 1 min time resolution using microdialysis coupled online to a Capillary HPLC system operating at about 500 bar and 50 °C. Several aspects of the system were optimized toward robust, in vivo online measurements. A two-loop, eight-port rotary injection valve demonstrated better consistency of continuous injections than the more commonly used two-loop, 10-port valve. A six-port loop injector for introducing stimulating solutions (stimulus injector) was placed in-line between the syringe pump and microdialysis probe. We minimized solute dispersion by using Capillary Tubing (75 μm inside diameter, 70 cm long) for the probe inlet and outlet. In vitro assessment of concentration dispersion during transport with a 30 s time resolution showed that the dispersion standard deviation for serotonin was well within the desired system temporal resolution. Each 30 or 60 s measurement reflects the integral of the true time response over the measurement time. We have accounted for this mathematically in determining the concentration dispersion during transport. The delay time between a concentration change at the probe and its detection is 7 min. The timing of injections from the stimulus injector and the cycle time for the HPLC monitoring of the flow stream were controlled. The electrochemical detector contained a 13 μm spacer to minimize detector dead volume. During in vivo experiments, retention time and separation efficiency were stable and reproducible. There was no statistically significant change over 5.5 h in the electrochemical detector sensitivity factor for serotonin. Dialysate serotonin concentrations change significantly in response to a 120 mM K(+) stimulus. Release of serotonin evoked by a 10 min, 120 mM K(+) stimulation, but not for other K(+) stimuli, exhibited a reproducible, oscillating profile of dialysate serotonin concentration versus time. Infusion of fluoxetine, a serotonin uptake inhibitor, increased dialysate serotonin concentrations and stimulated release magnitude. Transient serotonin increases were observed in response to the stress associated with unexpected handling. This system is simple, efficient, reliable, and suitable for the study of serotonin neurochemistry associated with emotion and behavior.
P S Belton - One of the best experts on this subject based on the ideXlab platform.
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thermal probe based analytical microscopy thermal analysis and photothermal fourier transform infrared microspectroscopy together with thermally assisted nanosampling coupled with Capillary electrophoresis
Analytical Chemistry, 2009Co-Authors: Xuan Dai, Jonathan Moffat, Andrew G Mayes, Mike Reading, Duncan Q M Craig, P S Belton, David B GrandyAbstract:In this study, we have demonstrated that a scanning probe microscope (SPM) can be used for thermally assisted nanosampling (TAN) with subsequent analysis by Capillary electrophoresis (CE). Localized thermomechanical analysis (L-TMA) and photothermal Fourier-transform infrared (PT-FTIR) microspectroscopy can also be employed using the same probe, thus illustrating how a single instrument can carry out a number of different complementary analytical measurements. Benzoic acid and 4-hydroxybenzoic acid were manipulated with a heated Wollaston wire probe and successfully deposited onto the surface of a piece of CE Capillary Tubing. The deposited samples were then separated with CE. L-TMA and PT-FTIR were also used to characterize these materials. We have also demonstrated how a nanosample of a nonparticulate material can be taken and then deposited onto the surface of an inert matrix. TAN of a nonparticulate material was explored using polyethylene as the analyte and fluorene as the matrix. These examples show...
Duncan Q M Craig - One of the best experts on this subject based on the ideXlab platform.
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thermal probe based analytical microscopy thermal analysis and photothermal fourier transform infrared microspectroscopy together with thermally assisted nanosampling coupled with Capillary electrophoresis
Analytical Chemistry, 2009Co-Authors: Xuan Dai, Jonathan Moffat, Andrew G Mayes, Mike Reading, Duncan Q M Craig, P S Belton, David B GrandyAbstract:In this study, we have demonstrated that a scanning probe microscope (SPM) can be used for thermally assisted nanosampling (TAN) with subsequent analysis by Capillary electrophoresis (CE). Localized thermomechanical analysis (L-TMA) and photothermal Fourier-transform infrared (PT-FTIR) microspectroscopy can also be employed using the same probe, thus illustrating how a single instrument can carry out a number of different complementary analytical measurements. Benzoic acid and 4-hydroxybenzoic acid were manipulated with a heated Wollaston wire probe and successfully deposited onto the surface of a piece of CE Capillary Tubing. The deposited samples were then separated with CE. L-TMA and PT-FTIR were also used to characterize these materials. We have also demonstrated how a nanosample of a nonparticulate material can be taken and then deposited onto the surface of an inert matrix. TAN of a nonparticulate material was explored using polyethylene as the analyte and fluorene as the matrix. These examples show...
