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Haruhiro Inoue - One of the best experts on this subject based on the ideXlab platform.
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background coloration of squamous epithelium in esophago pharyngeal squamous cell carcinoma what causes the color change
PLOS ONE, 2014Co-Authors: Hitomi Minami, Hajime Isomoto, Toshiyuki Nakayama, Tomayoshi Hayashi, Naoyuki Yamaguchi, Kayoko Matsushima, Yuko Akazawa, Ken Ohnita, Fuminao Takeshima, Haruhiro InoueAbstract:Objectives This study aims to clarify the cause of background coloration in the epithelia between each dilated intra papillary Capillary loop in esophago-pharyngeal squamous cell carcinoma.
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endoscopic diagnosis of tissue atypism ea in the pharyngeal and esophageal squamous epithelium ipcl pattern classification and eca classification
Kyobu geka. The Japanese journal of thoracic surgery, 2007Co-Authors: Haruhiro InoueAbstract:Abstract Standard magnifying endoscopy has around 100 fold magnifying power. Intra-epithelial papillary Capillary loop (IPCL) pattern is diagnosed with it. Narrow band imaging system (NBI) is extremely useful to recognize IPCL well as a brown spot. In IPCL type classification, type I mainly includes normal epithelium. Type Il corresponds to inflammatory change or non-neoplastic tissue and type Ill reflects border line lesions. Type IV strongly suggests carcinoma in situ. Type V-1 is definitely diagnosed as carcinoma in situ. Endocytoscopy has around 500 fold magnification, which enables observation of cell and nucleus. Endocytoscopic images are classified into 5 categories from normal epithelium to malignant tissue as endocytoscopic atypism classification (ECA classification). ECA IV and V are considered to be treated in clinical setting.
Luis Requena - One of the best experts on this subject based on the ideXlab platform.
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pathology of vascular skin lesions clinicopathologic correlations
2010Co-Authors: Omar P Sangueza, Luis RequenaAbstract:Companion CD-ROM Embryology, Anatomy, and Histology of the Vasculature of the Skin Embryologic Aspects Anatomic and Histologic Aspects of the Dermis and Blood Vessels Special Techniques for the Study of Vessels and Vascular Proliferations Immunohistochemical Stains Molecular Techniques Cytogenetic Studies Classification of Cutaneous Vascular Proliferations Cutaneous Vascular Hamartomas Phakomatosis Pigmentovascularis Eccrine Angiomatous Hamartoma Cutaneous Vascular Malformations Nevus Anemicus Cutis Marmorata Telangiectatica Congenita Nevus Flammeus Hyperkeratotic Vascular Stains Venous Malformations Superficial Cutaneous Lymphatic Malformations Cystic Lymphatic Malformations (Cystic Hygromas) Lymphangiomatosis Cutaneous Lesions Characterized by Dilation of Preexisting Vessels Spider Angioma (Nevus Araneus) Capillary Aneurysm-Venous Lake Telangiectases Angiokeratomas Lymphangiectases Cutaneous Vascular Hyperplasias Angiolymphoid Hyperplasia with Eosinophilia Pyogenic Granuloma Bacillary Angiomatosis Verruga Peruana Intravascular Papillary Endothelial Hyperplasia (Masson's Pseudo-Angiosarcoma) Pseudo-Kaposi's Sarcoma Reactive Angioendotheliomatosis Benign Neoplasms Angioma Serpiginosum Infantile Hemangiomas Cherry Angiomas (Senile Angiomas) Arteriovenous Hemangioma Hobnail Hemangioma (Targetoid Hemosiderotic Hemangioma) Microvenular Hemangioma Tufted Angioma Glomeruloid Hemangioma Acquired Elastotic Hemangioma Kaposiform Hemangioendothelioma Sinusoidal Hemangioma Giant Cell Angioblastoma Spindle Cell Hemangioma (Formerly Spindle Cell Hemangioendothelioma Benign Lymphangioendothelioma Benign Vascular Proliferations in Irradiated Skin Glomus Tumors Hemangiopericytoma Cutaneous Myofibroma Malignant Neoplasms Kaposi's Sarcoma Epithelioid Hemangioendothelioma Endovascular Papillary Angioendothelioma (Dabska's Tumor or Papillary Intralymphatic Angioendothelioma) Retiform Hemangioendothelioma Composite Hemangioendothelioma Cutaneous Angiosarcoma of the Face and Scalp of Elderly Patients (Wilson Jones' Angiosarcoma) Cutaneous Angiosarcoma Associated with Lymphedema Radiation-Induced Cutaneous Angiosarcoma Epithelioid Angiosarcoma Malignant Glomus Tumor (Glomangiosarcoma) Other Cutaneous Neoplasms With Significant Vascular Component Multinucleate Cell Angiohistiocytoma Angiofibroma Angioleiomyoma Angiolipoma Cutaneous Angiolipoleiomyoma Cutaneous Angiomyxoma Aggressive Angiomyxoma Disorders Erroneously Considered as Vascular Neoplasms Kimura's Disease 'Malignant' Angioendotheliomatosis (Intravascular Lymphomatosis) Acral Pseudolymphomatous Angiokeratoma in Children (APACHE) Index
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Superficial Spreading Capillary Hemangioma: Case Report
Actas Dermo-Sifiliográficas, 2007Co-Authors: E. González-guerra, M.r. Haro, Jorge Angulo, Lee J. Martin, M.c. Fariña, Luis RequenaAbstract:Superficial spreading Capillary hemangioma has been described for the first and sole time by Mihara and collaborators in 1986, as a proliferation of capillaries in the dermal papillae, separated by thin elongated rete ridges. Clinically it appeared as an asymptomatic, isolated, small and long-standing red plaque of slow growth located in the plantar surface of a 57-year-old Japanese woman. We report a 23-year-old Caucasian woman with an asymptomatic, long-standing, brownish plantar lesion of stable size. In the pathological study we observed a Capillary proliferation in tangles with slack stroma located in the papillary dermis. We report, therefore, the second case of superficial spreading Capillary hemangioma published in the literature.
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cutaneous vascular anomalies part i hamartomas malformations and dilatation of preexisting vessels
Journal of The American Academy of Dermatology, 1997Co-Authors: Luis Requena, Omar P SanguezaAbstract:Abstract Classification of cutaneous vascular anomalies is difficult because conceptual confusion persists between vascular neoplasms and malformations. However, hemangiomas of the infancy fulfill criteria both for hyperplasia and neoplasm because they result from proliferation of endothelial cells, but often undergo complete regression. Despite these pitfalls we have classified cutaneous vascular anomalies into the following categories: hamartomas, malformations, dilatations of preexisting vessels, hyperplasias, benign neoplasms, and malignant neoplasms. In this first part of our clinicopathologic review of vascular anomalies, hamartomas, malformations, and dilatation of preexisting vessels are covered. Hamartomas include several combined vascular and melanocytic proliferations grouped as phakomatosis pigmentovascularis and the so-called eccrine angiomatous hamartoma that consists of proliferations of both eccrine glands and blood vessels. Vascular malformations result from anomalies of embryologic development, and in some of them the abnormalities of the involved vessels are more functional than anatomic, as is the case of nevus anemicus. In contrast, other cutaneous vascular malformations show striking morphologic abnormalities of the vascular structures. These anatomic vascular malformations are subdivided into the following groups: Capillary, venous, arterial, lymphatic, and combined anomalies. Spider angioma, Capillary aneurysm–venous lake, and telangiectases are not vascular proliferations at all, but dilations of preexisting vessels. In our opinion, most of the lesions described with the generic term of "angiokeratoma" are not authentic vascular neoplasms, but hyperkeratotic malformations of capillaries and venules or acquired telangiectases of preexisting blood vessels of the papillary dermis. Therefore the first group of these "angiokeratomas" are included in the vascular malformations section, and the second group are covered in the section of dilation of preexisting vessels. Lymphangiectases are considered the lymphatic counterpart of angiokeratomas because they result from ectasia of preexisting lymphatic vessels of the papillary dermis. (J Am Acad Dermatol 1997;37:523-49.) Learning objective: At the completion of this learning activity participants should be familiar with vascular abnormalities in terms of classification, clinical characteristics, histopathologic features, differential diagnosis, and treatment. This article provides a comprehensive and updated review of the vascular hamartomas, malformations, and dilatation of preexisting vascular structures.
Franco Tagliaro - One of the best experts on this subject based on the ideXlab platform.
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fully automated analysis of carbohydrate deficient transferrin cdt by using a multiCapillary electrophoresis system
Clinica Chimica Acta, 2007Co-Authors: Federica Bortolotti, Giorgia De Paoli, Jennifer P Pascali, Franco TagliaroAbstract:Abstract Background The techniques universally believed as most reliable for determination of Carbohydrate-Deficient Transferrin (CDT) are HPLC and Capillary electrophoresis (CE). Recently a reagent kit for CDT analysis to be used in a multiCapillary electropherograph has been introduced. The present work was aimed at a validation of this new commercial system by application of the analytical chemistry parameters and by comparison with validated CE and HPLC methods. Methods One hundred forty serum samples were analyzed with multiCapillary CE (Capillarys™, Sebia, France) using kit reagents (CapillaryS™ CDT assay), with single Capillary electropherograph (P/ACE MDQ, Beckman Coulter, USA) using an original method developed by our group and with HPLC, performed on a gradient HPLC (Shimadzu Europe, Germany), using column and reagents provided in kit (ClinRep®, Recipe, Germany). Results The separation efficiency of the multiCapillary system was about 15,000 theoretical plates/column. The resolution of the transferrin isoforms ranged from 1.23 to 1.67. The variation coefficient was Conclusions The multiCapillary system shows high productivity with good analytical performances; however, a confirmation of positive results with an alternative method is required.
Maxim E. Darvin - One of the best experts on this subject based on the ideXlab platform.
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Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization
Scientific Reports, 2017Co-Authors: Evgeny A. Shirshin, Yury I. Gurfinkel, Alexander V. Priezzhev, Victor V. Fadeev, Juergen Lademann, Maxim E. DarvinAbstract:The papillary dermis of human skin is responsible for its biomechanical properties and for supply of epidermis with chemicals. Dermis is mainly composed of structural protein molecules, including collagen and elastin, and contains blood capillaries. Connective tissue diseases, as well as cardiovascular complications have manifestations on the molecular level in the papillary dermis (e.g. alteration of collagen I and III content) and in the Capillary structure. In this paper we assessed the molecular structure of internal and external regions of skin capillaries using two-photon fluorescence lifetime imaging (FLIM) of endogenous compounds. It was shown that the capillaries are characterized by a fast fluorescence decay, which is originated from red blood cells and blood plasma. Using the second harmonic generation signal, FLIM segmentation was performed, which provided for spatial localization and fluorescence decay parameters distribution of collagen I and elastin in the dermal papillae. It was demonstrated that the lifetime distribution was different for the inner area of dermal papillae around the Capillary loop that was suggested to be due to collagen III. Hence, we propose a generalized approach to two-photon imaging of the papillary dermis components, which extends the capabilities of this technique in skin diagnosis.
Jean Christophe Pages - One of the best experts on this subject based on the ideXlab platform.
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analytical evaluation of a new Capillary electrophoresis method for carbohydrate deficient transferrin measurement
Clinica Chimica Acta, 2007Co-Authors: Francois Schellenberg, Catherine Girre, B Nalpas, Arnaud Plat, Antonio Tome, Jean Christophe PagesAbstract:Abstract Background Carbohydrate-deficient transferrin (CDT), the sum of a- and disialotransferrin, is considered the most efficient routine biological marker of alcohol abuse. In recent years, methods based on Capillary zone electrophoresis (CZE) have been developed using specialized monoCapillary systems. These are characterized by a high analytical detection level, counterbalanced by a poor productivity. We evaluated a new CZE method for CDT measurement on the Sebia Capillarys®, an eight-Capillary system developed for routine serum Capillary electrophoresis. Methods Precision and possible biases due to abnormal (low or high) transferrin levels or lipemic samples were assessed. Exactitude and precision were tested by comparison with a HPLC procedure acknowledged to be the most reliable to date. The validity of the manufacturer's cut-off was checked by measuring CDT in a population comprising abstaining patients, moderate alcohol consumers and alcohol abusers. Lastly, the method was compared to the routine %CDT TIA and N Latex CDT methods. Results The imprecision was 18.5% at the minimum detection level and decreased to 6.1% for high CDT values. No significant shift in the CDT results was observed in relation to abnormally low or high serum transferrin, or in lipemic samples. A high level of concordance was observed with the HPLC method used as reference. The results were strongly correlated with both other routine methods ( r > 0.90). The diagnostic values were comparable to the literature data, even if differences in the studied populations make difficult a direct comparison of the results. Our data suggested that the cut-off could be raised from 1.3% to 1.4% to reduce the number of false positive values without loss of diagnostic efficiency. Conclusions This Capillarys® method from Sebia showed good precision as compared to those published using other CZE methods. Capillarys® method correlated well with HPLC and two routine methods. However, we noticed significant bias at low CDT concentrations. Therefore, with the advantage of high throughput and full automation, these results indicate that the new method is a consistent alternative to the other methods proposed for routine CDT measurement.
