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Paul A. Hartman - One of the best experts on this subject based on the ideXlab platform.
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aromatic amino acid biosynthesis and Carbohydrate Catabolism in strictly anaerobic mollicutes anaeroplasma spp
Systematic and Applied Microbiology, 1990Co-Authors: James P. Petzel, Paul A. HartmanAbstract:Summary Strictly anaerobic mollicutes (mycoplasmas) of the genus Anaeroplasma were examined for enzymic activities of aromatic amino acid and Carbohydrate metabolism. Anaeroplasma intermedium and Anaeroplasma varium possessed activities for four enzymes associated with aromatic amino acid biosynthesis: 7~phospho-2-dehydro-3-deoxy-D-arabino-heptonate synthase, shikimate dehydrogenase, prephenate dehydrogenase, and prephenate dehydratase. Shikimate dehydrogenase was detected with NAD+ but not NADP+. The effects of potential allosteric effectors on the activities of three of these enzymes were tested. Furthermore, Anaeroplasma bactoclasticum, Anaeroplasma varium, and Anaeroplasma abactoclasticum had enzymic activities of the Embden-Meyerhof-Parnas pathway for glycolytic Catabolism including PPi dependent phosphofructokinase. Anaeroplasma bactoclasticum and Anaeroplasma varium also possessed isocitrate dehydrogenase, an enzyme not detected in other mollicutes. In addition, FAD+-linked pyruvate synthase was detected in Anaeroplasma bactoclasticum and Anaeroplasma varium; this enzyme has not been previously reported to occur in mollicutes.
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Aromatic Amino Acid Biosynthesis and Carbohydrate Catabolism in Strictly Anaerobic Mollicutes (Anaeroplasma spp.)
Systematic and Applied Microbiology, 1990Co-Authors: James P. Petzel, Paul A. HartmanAbstract:Strictly anaerobic mollicutes (mycoplasmas) of the genus Anaeroplasma were examined for enzymic activities of aromatic amino acid and Carbohydrate metabolism. Anaeroplasma intermedium and Anaeroplasma varium possessed activities for four enzymes associated with aromatic amino acid biosynthesis: 7~phospho-2-dehydro-3-deoxy-D-arabino-heptonate synthase, shikimate dehydrogenase, prephenate dehydrogenase, and prephenate dehydratase. Shikimate dehydrogenase was detected with NAD+ but not NADP+. The effects of potential allosteric effectors on the activities of three of these enzymes were tested. Furthermore, Anaeroplasma bactoclasticum, Anaeroplasma varium, and Anaeroplasma abactoclasticum had enzymic activities of the Embden-Meyerhof-Parnas pathway for glycolytic Catabolism including PPi dependent phosphofructokinase. Anaeroplasma bactoclasticum and Anaeroplasma varium also possessed isocitrate dehydrogenase, an enzyme not detected in other mollicutes. In addition, FAD+-linked pyruvate synthase was detected in Anaeroplasma bactoclasticum and Anaeroplasma varium; this enzyme has not been previously reported to occur in mollicutes. © 1990, Gustav Fischer Verlag, Stuttgart · New York. All rights reserved.
James P. Petzel - One of the best experts on this subject based on the ideXlab platform.
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aromatic amino acid biosynthesis and Carbohydrate Catabolism in strictly anaerobic mollicutes anaeroplasma spp
Systematic and Applied Microbiology, 1990Co-Authors: James P. Petzel, Paul A. HartmanAbstract:Summary Strictly anaerobic mollicutes (mycoplasmas) of the genus Anaeroplasma were examined for enzymic activities of aromatic amino acid and Carbohydrate metabolism. Anaeroplasma intermedium and Anaeroplasma varium possessed activities for four enzymes associated with aromatic amino acid biosynthesis: 7~phospho-2-dehydro-3-deoxy-D-arabino-heptonate synthase, shikimate dehydrogenase, prephenate dehydrogenase, and prephenate dehydratase. Shikimate dehydrogenase was detected with NAD+ but not NADP+. The effects of potential allosteric effectors on the activities of three of these enzymes were tested. Furthermore, Anaeroplasma bactoclasticum, Anaeroplasma varium, and Anaeroplasma abactoclasticum had enzymic activities of the Embden-Meyerhof-Parnas pathway for glycolytic Catabolism including PPi dependent phosphofructokinase. Anaeroplasma bactoclasticum and Anaeroplasma varium also possessed isocitrate dehydrogenase, an enzyme not detected in other mollicutes. In addition, FAD+-linked pyruvate synthase was detected in Anaeroplasma bactoclasticum and Anaeroplasma varium; this enzyme has not been previously reported to occur in mollicutes.
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Aromatic Amino Acid Biosynthesis and Carbohydrate Catabolism in Strictly Anaerobic Mollicutes (Anaeroplasma spp.)
Systematic and Applied Microbiology, 1990Co-Authors: James P. Petzel, Paul A. HartmanAbstract:Strictly anaerobic mollicutes (mycoplasmas) of the genus Anaeroplasma were examined for enzymic activities of aromatic amino acid and Carbohydrate metabolism. Anaeroplasma intermedium and Anaeroplasma varium possessed activities for four enzymes associated with aromatic amino acid biosynthesis: 7~phospho-2-dehydro-3-deoxy-D-arabino-heptonate synthase, shikimate dehydrogenase, prephenate dehydrogenase, and prephenate dehydratase. Shikimate dehydrogenase was detected with NAD+ but not NADP+. The effects of potential allosteric effectors on the activities of three of these enzymes were tested. Furthermore, Anaeroplasma bactoclasticum, Anaeroplasma varium, and Anaeroplasma abactoclasticum had enzymic activities of the Embden-Meyerhof-Parnas pathway for glycolytic Catabolism including PPi dependent phosphofructokinase. Anaeroplasma bactoclasticum and Anaeroplasma varium also possessed isocitrate dehydrogenase, an enzyme not detected in other mollicutes. In addition, FAD+-linked pyruvate synthase was detected in Anaeroplasma bactoclasticum and Anaeroplasma varium; this enzyme has not been previously reported to occur in mollicutes. © 1990, Gustav Fischer Verlag, Stuttgart · New York. All rights reserved.
Frank Oliver Glockner - One of the best experts on this subject based on the ideXlab platform.
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habitat and taxon as driving forces of Carbohydrate Catabolism in marine heterotrophic bacteria example of the model algae associated bacterium zobellia galactanivorans dsijt
Environmental Microbiology, 2016Co-Authors: Tristan Barbeyron, Francois Thomas, Valerie Barbe, Hanno Teeling, Chantal Schenowitz, Carole Dossat, Alexander Goesmann, Catherine Leblanc, Frank Oliver GlocknerAbstract:Summary The marine flavobacterium Zobellia galactanivorans DsijT was isolated from a red alga and by now constitutes a model for studying algal polysaccharide bioconversions. We present an in-depth analysis of its complete genome and link it to physiological traits. Z. galactanivorans exhibited the highest gene numbers for glycoside hydrolases, polysaccharide lyases and Carbohydrate esterases and the second highest sulfatase gene number in a comparison to 125 other marine heterotrophic bacteria (MHB) genomes. Its genome contains 50 polysaccharide utilization loci, 22 of which contain sulfatase genes. Catabolic profiling confirmed a pronounced capacity for using algal polysaccharides and degradation of most polysaccharides could be linked to dedicated genes. Physiological and biochemical tests revealed that Z. galactanivorans stores and recycles glycogen, despite loss of several classic glycogen-related genes. Similar gene losses were observed in most Flavobacteriia, suggesting presence of an atypical glycogen metabolism in this class. Z. galactanivorans features numerous adaptive traits for algae-associated life, such as consumption of seaweed exudates, iodine metabolism and methylotrophy, indicating that this bacterium is well equipped to form profitable, stable interactions with macroalgae. Finally, using statistical and clustering analyses of the MHB genomes we show that their Carbohydrate Catabolism correlates with both taxonomy and habitat.
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Habitat and taxon as driving forces of Carbohydrate Catabolism in marine heterotrophic bacteria: example of the model algae‐associated bacterium Zobellia galactanivorans DsijT
Environmental Microbiology, 2016Co-Authors: Tristan Barbeyron, Francois Thomas, Valerie Barbe, Hanno Teeling, Chantal Schenowitz, Carole Dossat, Alexander Goesmann, Catherine Leblanc, Frank Oliver Glockner, Mirjam CzjzekAbstract:Summary The marine flavobacterium Zobellia galactanivorans DsijT was isolated from a red alga and by now constitutes a model for studying algal polysaccharide bioconversions. We present an in-depth analysis of its complete genome and link it to physiological traits. Z. galactanivorans exhibited the highest gene numbers for glycoside hydrolases, polysaccharide lyases and Carbohydrate esterases and the second highest sulfatase gene number in a comparison to 125 other marine heterotrophic bacteria (MHB) genomes. Its genome contains 50 polysaccharide utilization loci, 22 of which contain sulfatase genes. Catabolic profiling confirmed a pronounced capacity for using algal polysaccharides and degradation of most polysaccharides could be linked to dedicated genes. Physiological and biochemical tests revealed that Z. galactanivorans stores and recycles glycogen, despite loss of several classic glycogen-related genes. Similar gene losses were observed in most Flavobacteriia, suggesting presence of an atypical glycogen metabolism in this class. Z. galactanivorans features numerous adaptive traits for algae-associated life, such as consumption of seaweed exudates, iodine metabolism and methylotrophy, indicating that this bacterium is well equipped to form profitable, stable interactions with macroalgae. Finally, using statistical and clustering analyses of the MHB genomes we show that their Carbohydrate Catabolism correlates with both taxonomy and habitat.
P V Phibbs - One of the best experts on this subject based on the ideXlab platform.
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Carbohydrate Catabolism in pseudomonas aeruginosa
1998Co-Authors: Louise M Temple, A Sage, Herbert P Schweizer, P V PhibbsAbstract:The goal of this review is to update the reader on recent data elucidating the physiology and genetics of glycolytic pathways in P. aeruginosa, the most thoroughly investigated member of the pseudomonads. Glycolytic pathways in this organism have several unique features. Lacking phosphofructokinase, P. aeruginosa metabolizes three- and six-carbon sugars via a central cycle which includes the Entner-Doudoroff pathway (EDP) enzymes, rather than utilizing the fermentation pathway of Embden-Meyerhoff-Parnas (EMP) (Entner and Doudoroff, 1952; Kersters and DeLey, 1968). Another unique physiological feature is that a product of the EDP, glyceraldehyde 3-phosphate, is largely recycled through the central cycle, rather than continuing to pyuvate via the lower EMP pathway (Banerjee, 1989; Phibbs, 1988). Thus, the latter enzymes in P. aeruginosa seem to serve gluconeogenic rather than the more usual catabolic functions in other organisms. Whereas the metabolism of glucose is preferred by Escherichia coli, P. aeruginosa utilizes succinate and other tricarboxylic acid cycle intermediates before glucose (Anderson and Wood, 1969; Belvins et al., 1975; Hylemon and Phibbs, 1972; Midgley and Dawes, 1973; and Tiwari and Campbell, 1969). In addition, this organism lacks an oxidative hexose monophosphate pathway (Phibbs, 1988).
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two genes for Carbohydrate Catabolism are divergently transcribed from a region of dna containing the hexc locus in pseudomonas aeruginosa pao1
Journal of Bacteriology, 1994Co-Authors: Larissa K Temple, A Sage, Gail E Christie, P V PhibbsAbstract:The hexC locus of Pseudomonas aeruginosa PAO1 was localized to a 247-bp segment of chromosomal DNA on the multicopy broad-host-range vector pRO1614. The presence of this plasmid (pPZ196) in strain PAO1 produced the so-called "hexC effect," a two- to ninefold increase in the activities of four Carbohydrate Catabolism enzymes, glucokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydratase, and 2-keto-3-deoxy-6-phosphogluconate aldolase. The extent of the hexC effect was restricted, since three independently regulated metabolic enzymes were not affected by the presence of the hexC plasmid. Furthermore, the hexC-containing plasmid did not suppress catabolite repression control. Nucleotide sequence analysis of the segment of DNA encompassing hexC revealed a 128-bp region rich in adenosine-plus-thymine (AT) content separating two divergent open reading frames (ORFs). Transcriptional start sites for these two genes were mapped to the intergenic region, demonstrating that this sequence contained overlapping divergent promoters. The intergenic region contained potential regulatory sequences such as dyad symmetry motifs, polydeoxyadenosine tracts, and a sequence matching the integration host factor recognition site in Escherichia coli. One of the ORFs encoded a 610-amino-acid protein with 55 to 60% identity to 6-phosphogluconate dehydratase from E. coli and Zymomonas mobilis. The second ORF coded for a protein of 335 amino acids that displayed 45 to 60% identity to the NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAP) family of enzymes. The NAD-dependent GAP gene on the P. aeruginosa chromosome was previously unmapped. GAP was found to exhibit the hexC-dependent increase in its basal activity, establishing it as a fifth catabolic enzyme in the multioperonic hex regulon.
Tristan Barbeyron - One of the best experts on this subject based on the ideXlab platform.
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habitat and taxon as driving forces of Carbohydrate Catabolism in marine heterotrophic bacteria example of the model algae associated bacterium zobellia galactanivorans dsijt
Environmental Microbiology, 2016Co-Authors: Tristan Barbeyron, Francois Thomas, Valerie Barbe, Hanno Teeling, Chantal Schenowitz, Carole Dossat, Alexander Goesmann, Catherine Leblanc, Frank Oliver GlocknerAbstract:Summary The marine flavobacterium Zobellia galactanivorans DsijT was isolated from a red alga and by now constitutes a model for studying algal polysaccharide bioconversions. We present an in-depth analysis of its complete genome and link it to physiological traits. Z. galactanivorans exhibited the highest gene numbers for glycoside hydrolases, polysaccharide lyases and Carbohydrate esterases and the second highest sulfatase gene number in a comparison to 125 other marine heterotrophic bacteria (MHB) genomes. Its genome contains 50 polysaccharide utilization loci, 22 of which contain sulfatase genes. Catabolic profiling confirmed a pronounced capacity for using algal polysaccharides and degradation of most polysaccharides could be linked to dedicated genes. Physiological and biochemical tests revealed that Z. galactanivorans stores and recycles glycogen, despite loss of several classic glycogen-related genes. Similar gene losses were observed in most Flavobacteriia, suggesting presence of an atypical glycogen metabolism in this class. Z. galactanivorans features numerous adaptive traits for algae-associated life, such as consumption of seaweed exudates, iodine metabolism and methylotrophy, indicating that this bacterium is well equipped to form profitable, stable interactions with macroalgae. Finally, using statistical and clustering analyses of the MHB genomes we show that their Carbohydrate Catabolism correlates with both taxonomy and habitat.
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Habitat and taxon as driving forces of Carbohydrate Catabolism in marine heterotrophic bacteria: example of the model algae‐associated bacterium Zobellia galactanivorans DsijT
Environmental Microbiology, 2016Co-Authors: Tristan Barbeyron, Francois Thomas, Valerie Barbe, Hanno Teeling, Chantal Schenowitz, Carole Dossat, Alexander Goesmann, Catherine Leblanc, Frank Oliver Glockner, Mirjam CzjzekAbstract:Summary The marine flavobacterium Zobellia galactanivorans DsijT was isolated from a red alga and by now constitutes a model for studying algal polysaccharide bioconversions. We present an in-depth analysis of its complete genome and link it to physiological traits. Z. galactanivorans exhibited the highest gene numbers for glycoside hydrolases, polysaccharide lyases and Carbohydrate esterases and the second highest sulfatase gene number in a comparison to 125 other marine heterotrophic bacteria (MHB) genomes. Its genome contains 50 polysaccharide utilization loci, 22 of which contain sulfatase genes. Catabolic profiling confirmed a pronounced capacity for using algal polysaccharides and degradation of most polysaccharides could be linked to dedicated genes. Physiological and biochemical tests revealed that Z. galactanivorans stores and recycles glycogen, despite loss of several classic glycogen-related genes. Similar gene losses were observed in most Flavobacteriia, suggesting presence of an atypical glycogen metabolism in this class. Z. galactanivorans features numerous adaptive traits for algae-associated life, such as consumption of seaweed exudates, iodine metabolism and methylotrophy, indicating that this bacterium is well equipped to form profitable, stable interactions with macroalgae. Finally, using statistical and clustering analyses of the MHB genomes we show that their Carbohydrate Catabolism correlates with both taxonomy and habitat.