The Experts below are selected from a list of 177 Experts worldwide ranked by ideXlab platform
Jarone Pinhassi - One of the best experts on this subject based on the ideXlab platform.
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Structuring of bacterioplankton communities by specific dissolved organic Carbon Compounds
Environmental Microbiology, 2012Co-Authors: Laura Gómez-consarnau, Markus V. Lindh, Josep M. Gasol, Jarone PinhassiAbstract:Summary The main role of microorganisms in the cycling of the bulk dissolved organic Carbon pool in the ocean is well established. Nevertheless, it remains unclear if particular bacteria preferentially utilize specific Carbon Compounds and whether such Compounds have the potential to shape bacterial community com- position. Enrichment experiments in the Mediterra- nean Sea, Baltic Sea and the North Sea (Skagerrak) showed that different low-molecular-weight organic Compounds, with a proven importance for the growth of marine bacteria (e.g. amino acids, glucose, dimeth- ylsulphoniopropionate, acetate or pyruvate), in most cases differentially stimulated bacterial growth. Dena- turing gradient gel electrophoresis 'fingerprints' and 16S rRNA gene sequencing revealed that some bac- terial phylotypes that became abundant were highly specific to enrichment with specific Carbon com- pounds (e.g. Acinetobacter sp. B1-A3 with acetate or Psychromonas sp. B3-U1 with glucose). In contrast, other phylotypes increased in relative abundance in response to enrichment with several, or all, of the investigated Carbon Compounds (e.g. Neptuniibacter sp. M2-A4 with acetate, pyruvate and dimethylsulpho- niopropionate, and Thalassobacter sp. M3-A3 with pyruvate and amino acids). Furthermore, different Carbon Compounds triggered the development of unique combinations of dominant phylotypes in several of the experiments. These results suggest that bacteria differ substantially in their abilities to utilize specific Carbon Compounds, with some bacte- ria being specialists and others having a more gener- alist strategy. Thus, changes in the supply or composition of the dissolved organic Carbon pool can act as selective forces structuring bacterioplankton communities.
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Structuring of bacterioplankton communities by specific dissolved organic Carbon Compounds
Environmental Microbiology, 2012Co-Authors: Laura Gómez-consarnau, Markus V. Lindh, Josep M. Gasol, Jarone PinhassiAbstract:Summary The main role of microorganisms in the cycling of the bulk dissolved organic Carbon pool in the ocean is well established. Nevertheless, it remains unclear if particular bacteria preferentially utilize specific Carbon Compounds and whether such Compounds have the potential to shape bacterial community com- position. Enrichment experiments in the Mediterra- nean Sea, Baltic Sea and the North Sea (Skagerrak) showed that different low-molecular-weight organic Compounds, with a proven importance for the growth of marine bacteria (e.g. amino acids, glucose, dimeth- ylsulphoniopropionate, acetate or pyruvate), in most cases differentially stimulated bacterial growth. Dena- turing gradient gel electrophoresis 'fingerprints' and 16S rRNA gene sequencing revealed that some bac- terial phylotypes that became abundant were highly specific to enrichment with specific Carbon com- pounds (e.g. Acinetobacter sp. B1-A3 with acetate or Psychromonas sp. B3-U1 with glucose). In contrast, other phylotypes increased in relative abundance in response to enrichment with several, or all, of the investigated Carbon Compounds (e.g. Neptuniibacter sp. M2-A4 with acetate, pyruvate and dimethylsulpho- niopropionate, and Thalassobacter sp. M3-A3 with pyruvate and amino acids). Furthermore, different Carbon Compounds triggered the development of unique combinations of dominant phylotypes in several of the experiments. These results suggest that bacteria differ substantially in their abilities to utilize specific Carbon Compounds, with some bacte- ria being specialists and others having a more gener- alist strategy. Thus, changes in the supply or composition of the dissolved organic Carbon pool can act as selective forces structuring bacterioplankton communities.
James C. Liao - One of the best experts on this subject based on the ideXlab platform.
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A modified serine cycle in Escherichia coli coverts methanol and CO_2 to two-Carbon Compounds
Nature Communications, 2018Co-Authors: James C. LiaoAbstract:Microbial utilization of renewable one-Carbon Compounds, such as methane, methanol, formic acid, and CO_2, has emerged as a potential approach to increase the range of Carbon sources for bioproduction and address climate change issues. Here, we modify the natural serine cycle present in methylotrophs and build an adapted pathway for Escherichia coli , which allows microorganism to condense methanol (or formate) together with biCarbonate to produce various products. We introduce the modified cycle into E. coli and demonstrate its capability for one-Carbon assimilation through growth complementation and isotope labeling experiments. We also demonstrate conversion of methanol to ethanol by utilizing the modified serine cycle in an engineered E. coli strain, achieving a reaction yet to be accomplished by a one-pot chemical process. This work provides a platform to utilize various renewable one-Carbon Compounds as Carbon sources for biosynthesis through a modified serine cycle in E. coli . Assimilating the abundant one-Carbon Compounds by an industrially-relevant microorganism can broaden the substrate range and achieve reactions that are difficult for chemical process. Here the authors show a modified serine cycle can convert methanol and CO_2 to two-Carbon Compounds in an engineered E. coli strain.
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A modified serine cycle in Escherichia coli coverts methanol and CO 2 to two-Carbon Compounds
Nature communications, 2018Co-Authors: James C. LiaoAbstract:Microbial utilization of renewable one-Carbon Compounds, such as methane, methanol, formic acid, and CO2, has emerged as a potential approach to increase the range of Carbon sources for bioproduction and address climate change issues. Here, we modify the natural serine cycle present in methylotrophs and build an adapted pathway for Escherichia coli, which allows microorganism to condense methanol (or formate) together with biCarbonate to produce various products. We introduce the modified cycle into E. coli and demonstrate its capability for one-Carbon assimilation through growth complementation and isotope labeling experiments. We also demonstrate conversion of methanol to ethanol by utilizing the modified serine cycle in an engineered E. coli strain, achieving a reaction yet to be accomplished by a one-pot chemical process. This work provides a platform to utilize various renewable one-Carbon Compounds as Carbon sources for biosynthesis through a modified serine cycle in E. coli.
Laura Gómez-consarnau - One of the best experts on this subject based on the ideXlab platform.
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Structuring of bacterioplankton communities by specific dissolved organic Carbon Compounds
Environmental Microbiology, 2012Co-Authors: Laura Gómez-consarnau, Markus V. Lindh, Josep M. Gasol, Jarone PinhassiAbstract:Summary The main role of microorganisms in the cycling of the bulk dissolved organic Carbon pool in the ocean is well established. Nevertheless, it remains unclear if particular bacteria preferentially utilize specific Carbon Compounds and whether such Compounds have the potential to shape bacterial community com- position. Enrichment experiments in the Mediterra- nean Sea, Baltic Sea and the North Sea (Skagerrak) showed that different low-molecular-weight organic Compounds, with a proven importance for the growth of marine bacteria (e.g. amino acids, glucose, dimeth- ylsulphoniopropionate, acetate or pyruvate), in most cases differentially stimulated bacterial growth. Dena- turing gradient gel electrophoresis 'fingerprints' and 16S rRNA gene sequencing revealed that some bac- terial phylotypes that became abundant were highly specific to enrichment with specific Carbon com- pounds (e.g. Acinetobacter sp. B1-A3 with acetate or Psychromonas sp. B3-U1 with glucose). In contrast, other phylotypes increased in relative abundance in response to enrichment with several, or all, of the investigated Carbon Compounds (e.g. Neptuniibacter sp. M2-A4 with acetate, pyruvate and dimethylsulpho- niopropionate, and Thalassobacter sp. M3-A3 with pyruvate and amino acids). Furthermore, different Carbon Compounds triggered the development of unique combinations of dominant phylotypes in several of the experiments. These results suggest that bacteria differ substantially in their abilities to utilize specific Carbon Compounds, with some bacte- ria being specialists and others having a more gener- alist strategy. Thus, changes in the supply or composition of the dissolved organic Carbon pool can act as selective forces structuring bacterioplankton communities.
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Structuring of bacterioplankton communities by specific dissolved organic Carbon Compounds
Environmental Microbiology, 2012Co-Authors: Laura Gómez-consarnau, Markus V. Lindh, Josep M. Gasol, Jarone PinhassiAbstract:Summary The main role of microorganisms in the cycling of the bulk dissolved organic Carbon pool in the ocean is well established. Nevertheless, it remains unclear if particular bacteria preferentially utilize specific Carbon Compounds and whether such Compounds have the potential to shape bacterial community com- position. Enrichment experiments in the Mediterra- nean Sea, Baltic Sea and the North Sea (Skagerrak) showed that different low-molecular-weight organic Compounds, with a proven importance for the growth of marine bacteria (e.g. amino acids, glucose, dimeth- ylsulphoniopropionate, acetate or pyruvate), in most cases differentially stimulated bacterial growth. Dena- turing gradient gel electrophoresis 'fingerprints' and 16S rRNA gene sequencing revealed that some bac- terial phylotypes that became abundant were highly specific to enrichment with specific Carbon com- pounds (e.g. Acinetobacter sp. B1-A3 with acetate or Psychromonas sp. B3-U1 with glucose). In contrast, other phylotypes increased in relative abundance in response to enrichment with several, or all, of the investigated Carbon Compounds (e.g. Neptuniibacter sp. M2-A4 with acetate, pyruvate and dimethylsulpho- niopropionate, and Thalassobacter sp. M3-A3 with pyruvate and amino acids). Furthermore, different Carbon Compounds triggered the development of unique combinations of dominant phylotypes in several of the experiments. These results suggest that bacteria differ substantially in their abilities to utilize specific Carbon Compounds, with some bacte- ria being specialists and others having a more gener- alist strategy. Thus, changes in the supply or composition of the dissolved organic Carbon pool can act as selective forces structuring bacterioplankton communities.
Josep M. Gasol - One of the best experts on this subject based on the ideXlab platform.
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Structuring of bacterioplankton communities by specific dissolved organic Carbon Compounds
Environmental Microbiology, 2012Co-Authors: Laura Gómez-consarnau, Markus V. Lindh, Josep M. Gasol, Jarone PinhassiAbstract:Summary The main role of microorganisms in the cycling of the bulk dissolved organic Carbon pool in the ocean is well established. Nevertheless, it remains unclear if particular bacteria preferentially utilize specific Carbon Compounds and whether such Compounds have the potential to shape bacterial community com- position. Enrichment experiments in the Mediterra- nean Sea, Baltic Sea and the North Sea (Skagerrak) showed that different low-molecular-weight organic Compounds, with a proven importance for the growth of marine bacteria (e.g. amino acids, glucose, dimeth- ylsulphoniopropionate, acetate or pyruvate), in most cases differentially stimulated bacterial growth. Dena- turing gradient gel electrophoresis 'fingerprints' and 16S rRNA gene sequencing revealed that some bac- terial phylotypes that became abundant were highly specific to enrichment with specific Carbon com- pounds (e.g. Acinetobacter sp. B1-A3 with acetate or Psychromonas sp. B3-U1 with glucose). In contrast, other phylotypes increased in relative abundance in response to enrichment with several, or all, of the investigated Carbon Compounds (e.g. Neptuniibacter sp. M2-A4 with acetate, pyruvate and dimethylsulpho- niopropionate, and Thalassobacter sp. M3-A3 with pyruvate and amino acids). Furthermore, different Carbon Compounds triggered the development of unique combinations of dominant phylotypes in several of the experiments. These results suggest that bacteria differ substantially in their abilities to utilize specific Carbon Compounds, with some bacte- ria being specialists and others having a more gener- alist strategy. Thus, changes in the supply or composition of the dissolved organic Carbon pool can act as selective forces structuring bacterioplankton communities.
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Structuring of bacterioplankton communities by specific dissolved organic Carbon Compounds
Environmental Microbiology, 2012Co-Authors: Laura Gómez-consarnau, Markus V. Lindh, Josep M. Gasol, Jarone PinhassiAbstract:Summary The main role of microorganisms in the cycling of the bulk dissolved organic Carbon pool in the ocean is well established. Nevertheless, it remains unclear if particular bacteria preferentially utilize specific Carbon Compounds and whether such Compounds have the potential to shape bacterial community com- position. Enrichment experiments in the Mediterra- nean Sea, Baltic Sea and the North Sea (Skagerrak) showed that different low-molecular-weight organic Compounds, with a proven importance for the growth of marine bacteria (e.g. amino acids, glucose, dimeth- ylsulphoniopropionate, acetate or pyruvate), in most cases differentially stimulated bacterial growth. Dena- turing gradient gel electrophoresis 'fingerprints' and 16S rRNA gene sequencing revealed that some bac- terial phylotypes that became abundant were highly specific to enrichment with specific Carbon com- pounds (e.g. Acinetobacter sp. B1-A3 with acetate or Psychromonas sp. B3-U1 with glucose). In contrast, other phylotypes increased in relative abundance in response to enrichment with several, or all, of the investigated Carbon Compounds (e.g. Neptuniibacter sp. M2-A4 with acetate, pyruvate and dimethylsulpho- niopropionate, and Thalassobacter sp. M3-A3 with pyruvate and amino acids). Furthermore, different Carbon Compounds triggered the development of unique combinations of dominant phylotypes in several of the experiments. These results suggest that bacteria differ substantially in their abilities to utilize specific Carbon Compounds, with some bacte- ria being specialists and others having a more gener- alist strategy. Thus, changes in the supply or composition of the dissolved organic Carbon pool can act as selective forces structuring bacterioplankton communities.
Markus V. Lindh - One of the best experts on this subject based on the ideXlab platform.
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Structuring of bacterioplankton communities by specific dissolved organic Carbon Compounds
Environmental Microbiology, 2012Co-Authors: Laura Gómez-consarnau, Markus V. Lindh, Josep M. Gasol, Jarone PinhassiAbstract:Summary The main role of microorganisms in the cycling of the bulk dissolved organic Carbon pool in the ocean is well established. Nevertheless, it remains unclear if particular bacteria preferentially utilize specific Carbon Compounds and whether such Compounds have the potential to shape bacterial community com- position. Enrichment experiments in the Mediterra- nean Sea, Baltic Sea and the North Sea (Skagerrak) showed that different low-molecular-weight organic Compounds, with a proven importance for the growth of marine bacteria (e.g. amino acids, glucose, dimeth- ylsulphoniopropionate, acetate or pyruvate), in most cases differentially stimulated bacterial growth. Dena- turing gradient gel electrophoresis 'fingerprints' and 16S rRNA gene sequencing revealed that some bac- terial phylotypes that became abundant were highly specific to enrichment with specific Carbon com- pounds (e.g. Acinetobacter sp. B1-A3 with acetate or Psychromonas sp. B3-U1 with glucose). In contrast, other phylotypes increased in relative abundance in response to enrichment with several, or all, of the investigated Carbon Compounds (e.g. Neptuniibacter sp. M2-A4 with acetate, pyruvate and dimethylsulpho- niopropionate, and Thalassobacter sp. M3-A3 with pyruvate and amino acids). Furthermore, different Carbon Compounds triggered the development of unique combinations of dominant phylotypes in several of the experiments. These results suggest that bacteria differ substantially in their abilities to utilize specific Carbon Compounds, with some bacte- ria being specialists and others having a more gener- alist strategy. Thus, changes in the supply or composition of the dissolved organic Carbon pool can act as selective forces structuring bacterioplankton communities.
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Structuring of bacterioplankton communities by specific dissolved organic Carbon Compounds
Environmental Microbiology, 2012Co-Authors: Laura Gómez-consarnau, Markus V. Lindh, Josep M. Gasol, Jarone PinhassiAbstract:Summary The main role of microorganisms in the cycling of the bulk dissolved organic Carbon pool in the ocean is well established. Nevertheless, it remains unclear if particular bacteria preferentially utilize specific Carbon Compounds and whether such Compounds have the potential to shape bacterial community com- position. Enrichment experiments in the Mediterra- nean Sea, Baltic Sea and the North Sea (Skagerrak) showed that different low-molecular-weight organic Compounds, with a proven importance for the growth of marine bacteria (e.g. amino acids, glucose, dimeth- ylsulphoniopropionate, acetate or pyruvate), in most cases differentially stimulated bacterial growth. Dena- turing gradient gel electrophoresis 'fingerprints' and 16S rRNA gene sequencing revealed that some bac- terial phylotypes that became abundant were highly specific to enrichment with specific Carbon com- pounds (e.g. Acinetobacter sp. B1-A3 with acetate or Psychromonas sp. B3-U1 with glucose). In contrast, other phylotypes increased in relative abundance in response to enrichment with several, or all, of the investigated Carbon Compounds (e.g. Neptuniibacter sp. M2-A4 with acetate, pyruvate and dimethylsulpho- niopropionate, and Thalassobacter sp. M3-A3 with pyruvate and amino acids). Furthermore, different Carbon Compounds triggered the development of unique combinations of dominant phylotypes in several of the experiments. These results suggest that bacteria differ substantially in their abilities to utilize specific Carbon Compounds, with some bacte- ria being specialists and others having a more gener- alist strategy. Thus, changes in the supply or composition of the dissolved organic Carbon pool can act as selective forces structuring bacterioplankton communities.