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Teruo Ogawa - One of the best experts on this subject based on the ideXlab platform.

  • Identification and Characterization of the ictA/ndhL Gene Product Essential to Inorganic Carbon Transport of Synechocystis PCC6803.
    Plant physiology, 1992
    Co-Authors: Teruo Ogawa
    Abstract:

    The ictA gene, renamed ndhL in this paper, essential to inorganic Carbon Transport of Synechocystis PCC6803, was expressed in Eschericia coli as a fusion protein with glutathione S-transferase. An antibody was raised against this fusion protein. Western analysis of the thylakoid membrane of wild-type (WT) Synechocystis revealed that a protein with an apparent molecular mass of 6.7 kilodaltons cross-reacted with this antibody. No immunoreactive protein was present in the thylakoid membranes of the Synechocystis mutants, RKb and M9, which have defects in the ictA/ndhL gene, or in the cytoplasmic membranes of the WT and mutant cells. Thus, the protein reacted with the antibody is the ictA gene product (IctA) and is localized in the thylakoid membrane of WT cells. IctA was absent in the thylakoid membranes of the M55 mutant, in which the ndhB gene is inactivated, and was poorly immunostained in the membranes of the mutants (M-ndhC and M-ndhK) constructed by inactivating the ndhC and ndhK genes of WT Synechocystis, respectively. The Carbon dioxide uptake activity was nearly zero in M-ndhK and was about 40% of the activity of WT cells in M-ndhC. The RKb, M-ndhC, and M-ndhK mutants were unable to grow or grew very slowly under photoheterotrophic conditions. These results indicated that NADH dehydrogenase is essential to inorganic Carbon Transport and photoheterotrophic growth of Synechocystis and that IctA is one of the subunits of this enzyme.

  • Cloning and Inactivation of a Gene Essential to Inorganic Carbon Transport of Synechocystis PCC6803.
    Plant physiology, 1991
    Co-Authors: Teruo Ogawa
    Abstract:

    A clone (HP-1) which transforms the high CO2-requiring mutant (RKb) of Synechocystis PCC6803 defective in inorganic Carbon Transport to the wild-type (WT) phenotype was isolated from a WT genomic library. The clone contained a 5.4 kilobase-pair DNA insert. Complementation tests with subclones derived from HP-1 allowed the mutation in RKb to be located within 141 base-pair nucleotides. Sequencing of nucleotides in this region revealed an open reading frame encoding a hydrophobic protein consists of 80 amino acids. A defined mutant (M9) constructed by inactivating this putative inorganic Carbon Transport gene, designated ictA, was unable to Transport CO2 and HCO3− into the intracellular inorganic Carbon pool. Cloning and sequence analysis of the respective RKb gene revealed a base substitution which generates a stop codon in the middle of ictA.

Jessica A Savage - One of the best experts on this subject based on the ideXlab platform.

Zhao Xiancha - One of the best experts on this subject based on the ideXlab platform.

  • Analysis on the Status and Development Path of the China's Urban Low-Carbon Transport
    Urban Studies, 2011
    Co-Authors: Zhao Xiancha
    Abstract:

    The paper elaborates the urban low-Carbon Transport from the aspects of definition and connotation,and analyzes the situation of Carbon emission and energy consumption of the China's urban low-Carbon Transport form 2000 to now,the results indicate:rapid growth of energy consumption and CO2 emissions of the China's urban Transport,and the Carbon emission intensity of the public Transport being the lowest in motorized Transport,and major problems of development of the China's urban low-Carbon Transport being summarized:lag of the development of the public Transport,chang of the citizen travel structure,diminution of non-motorized Transport,lack of effective demand management and awareness of energy-saving and Carbon emission reduction,and urban traffic management system being unsuitable to China's urban low-Carbon Transport construction.On this basis above,three strategic directions and four main measures of China's low-Carbon Transport construction are proposed.

Cankui Zhang - One of the best experts on this subject based on the ideXlab platform.

  • allocation stress tolerance and Carbon Transport in plants how does phloem physiology affect plant ecology
    Plant Cell and Environment, 2016
    Co-Authors: Jessica A Savage, Maurizio Mencuccini, Sanna Sevanto, Robert Turgeon, Michael J Clearwater, Dustin F Haines, Tamir Klein, Cankui Zhang
    Abstract:

    Despite the crucial role of Carbon Transport in whole plant physiology and its impact on plant–environment interactions and ecosystem function, relatively little research has tried to examine how phloem physiology impacts plant ecology. In this review, we highlight several areas of active research where inquiry into phloem physiology has increased our understanding of whole plant function and ecological processes. We consider how xylem–phloem interactions impact plant drought tolerance and reproduction, how phloem Transport influences Carbon allocation in trees and Carbon cycling in ecosystems and how phloem function mediates plant relations with insects, pests, microbes and symbiotes. We argue that in spite of challenges that exist in studying phloem physiology, it is critical that we consider the role of this dynamic vascular system when examining the relationship between plants and their biotic and abiotic environment.

  • Allocation, stress tolerance and Carbon Transport in plants: how does phloem physiology affect plant ecology?: Phloem ecophysiology
    Plant cell & environment, 2015
    Co-Authors: Jessica A Savage, Maurizio Mencuccini, Sanna Sevanto, Robert Turgeon, Michael J Clearwater, Dustin F Haines, Tamir Klein, Cankui Zhang
    Abstract:

    Despite the crucial role of Carbon Transport in whole plant physiology and its impact on plant-environment interactions and ecosystem function, relatively little research has tried to examine how phloem physiology impacts plant ecology. In this review, we highlight several areas of active research where inquiry into phloem physiology has increased our understanding of whole plant function and ecological processes. We consider how xylem-phloem interactions impact plant drought tolerance and reproduction, how phloem Transport influences Carbon allocation in trees and Carbon cycling in ecosystems and how phloem function mediates plant relations with insects, pests, microbes and symbiotes. We argue that in spite of challenges that exist in studying phloem physiology, it is critical that we consider the role of this dynamic vascular system when examining the relationship between plants and their biotic and abiotic environment.

Sanna Sevanto - One of the best experts on this subject based on the ideXlab platform.

  • allocation stress tolerance and Carbon Transport in plants how does phloem physiology affect plant ecology
    Plant Cell and Environment, 2016
    Co-Authors: Jessica A Savage, Maurizio Mencuccini, Sanna Sevanto, Robert Turgeon, Michael J Clearwater, Dustin F Haines, Tamir Klein, Cankui Zhang
    Abstract:

    Despite the crucial role of Carbon Transport in whole plant physiology and its impact on plant–environment interactions and ecosystem function, relatively little research has tried to examine how phloem physiology impacts plant ecology. In this review, we highlight several areas of active research where inquiry into phloem physiology has increased our understanding of whole plant function and ecological processes. We consider how xylem–phloem interactions impact plant drought tolerance and reproduction, how phloem Transport influences Carbon allocation in trees and Carbon cycling in ecosystems and how phloem function mediates plant relations with insects, pests, microbes and symbiotes. We argue that in spite of challenges that exist in studying phloem physiology, it is critical that we consider the role of this dynamic vascular system when examining the relationship between plants and their biotic and abiotic environment.

  • Allocation, stress tolerance and Carbon Transport in plants: how does phloem physiology affect plant ecology?: Phloem ecophysiology
    Plant cell & environment, 2015
    Co-Authors: Jessica A Savage, Maurizio Mencuccini, Sanna Sevanto, Robert Turgeon, Michael J Clearwater, Dustin F Haines, Tamir Klein, Cankui Zhang
    Abstract:

    Despite the crucial role of Carbon Transport in whole plant physiology and its impact on plant-environment interactions and ecosystem function, relatively little research has tried to examine how phloem physiology impacts plant ecology. In this review, we highlight several areas of active research where inquiry into phloem physiology has increased our understanding of whole plant function and ecological processes. We consider how xylem-phloem interactions impact plant drought tolerance and reproduction, how phloem Transport influences Carbon allocation in trees and Carbon cycling in ecosystems and how phloem function mediates plant relations with insects, pests, microbes and symbiotes. We argue that in spite of challenges that exist in studying phloem physiology, it is critical that we consider the role of this dynamic vascular system when examining the relationship between plants and their biotic and abiotic environment.