The Experts below are selected from a list of 87 Experts worldwide ranked by ideXlab platform

Karlerich Jaeger - One of the best experts on this subject based on the ideXlab platform.

  • identification of organic solvent tolerant Carboxylic Ester Hydrolases for organic synthesis
    Applied and Environmental Microbiology, 2020
    Co-Authors: Alexander Bollinger, Rebecka Molitor, Stephan Thies, Rainhard Koch, Cristina Coscolin, Manuel Ferrer, Karlerich Jaeger
    Abstract:

    Biocatalysis has emerged as an important tool in synthetic organic chemistry enabling the chemical industry to execute reactions with high regio- or enantioselectivity and under usually mild reaction conditions while avoiding toxic waste. Target substrates and products of reactions catalyzed by Carboxylic Ester Hydrolases are often poorly water soluble and require organic solvents, whereas enzymes are evolved by nature to be active in cells, i.e., in aqueous rather than organic solvents. Therefore, biocatalysts that withstand organic solvents are urgently needed. Current strategies to identify such enzymes rely on laborious tests carried out by incubation in different organic solvents and determination of residual activity. Here, we describe a simple assay useful for screening large libraries of Carboxylic Ester Hydrolases for resistance and activity in water-miscible organic solvents. We have screened a set of 26 enzymes, most of them identified in this study, with four different water-miscible organic solvents. The triglyceride tributyrin was used as a substrate, and fatty acids released by enzymatic hydrolysis were detected by a pH shift indicated by the indicator dye nitrazine yellow. With this strategy, we succeeded in identifying a novel highly organic-solvent-tolerant Esterase from Pseudomonas aestusnigri In addition, the newly identified enzymes were tested with sterically demanding substrates, which are common in pharmaceutical intermediates, and two enzymes from Alcanivorax borkumensis were identified which outcompeted the gold standard Ester hydrolase CalB from Candida antarctica IMPORTANCE Major challenges hampering biotechnological applications of Esterases include the requirement to accept nonnatural and chemically demanding substrates and the tolerance of the enzymes toward organic solvents which are often required to solubilize such substrates. We describe here a high-throughput screening strategy to identify novel organic-solvent-tolerant Carboxylic Ester Hydrolases (CEs). Among these enzymes, CEs active against water-insoluble bulky substrates were identified. Our results thus contribute to fostering the identification and biotechnological application of CEs.

  • Identification of organic solvent tolerant Carboxylic Ester Hydrolases for organic synthesis.
    Applied and Environmental Microbiology, 2020
    Co-Authors: Alexander Bollinger, Rebecka Molitor, Stephan Thies, Rainhard Koch, Cristina Coscolin, Manuel Ferrer, Karlerich Jaeger
    Abstract:

    Biocatalysis has emerged as an important tool in synthetic organic chemistry enabling the chemical industry to execute reactions with high regio- or enantioselectivity and under usually mild reaction conditions while avoiding toxic waste. Target substrates and products of reactions catalyzed by Carboxylic Ester Hydrolases are often poorly water-soluble and require organic solvents, enzymes are evolved by nature to be active in cells, i.e. in aqueous rather than organic solvents. Therefore, biocatalysts withstanding organic solvents are urgently needed. Current strategies to identify such enzymes rely on laborious tests carried out by incubation in different organic solvents and determination of residual activities. Here, we describe a simple assay useful to screen large libraries of Carboxylic Ester Hydrolases for resistance and activity in water miscible organic solvents. We have screened a set of 26 enzymes, most of them identified in this study, with four different water miscible organic solvents. The triglyceride tributyrin was used as a substrate and fatty acids released by enzymatic hydrolysis were detected by a pH shift indicated by the indicator dye nitrazine yellow. With this strategy, we succeeded to identify a novel highly organic solvent tolerant Esterase from Pseudomonas aestusnigri. In addition, the newly identified enzymes were tested with sterically demanding substrates, as common in pharmaceutical intermediates, and two enzymes from Alcanivorax borkumensis were identified which outcompeted the gold standard Ester hydrolase CalB from Candida antarctica. Importance Major challenges hampering biotechnological applications of Esterases include the requirement to accept non-natural and chemically demanding substrates and tolerance of the enzymes towards organic solvents which are often required to solubilize such substrates. We describe here a high throughput screening strategy to identify novel organic solvent tolerant Carboxylic Ester Hydrolases (CEs). Among these enzymes, CEs active against water-insoluble bulky substrates were identified. Our results thus contribute to fostering the identification and biotechnological application of CEs.

Alexander Bollinger - One of the best experts on this subject based on the ideXlab platform.

  • identification of organic solvent tolerant Carboxylic Ester Hydrolases for organic synthesis
    Applied and Environmental Microbiology, 2020
    Co-Authors: Alexander Bollinger, Rebecka Molitor, Stephan Thies, Rainhard Koch, Cristina Coscolin, Manuel Ferrer, Karlerich Jaeger
    Abstract:

    Biocatalysis has emerged as an important tool in synthetic organic chemistry enabling the chemical industry to execute reactions with high regio- or enantioselectivity and under usually mild reaction conditions while avoiding toxic waste. Target substrates and products of reactions catalyzed by Carboxylic Ester Hydrolases are often poorly water soluble and require organic solvents, whereas enzymes are evolved by nature to be active in cells, i.e., in aqueous rather than organic solvents. Therefore, biocatalysts that withstand organic solvents are urgently needed. Current strategies to identify such enzymes rely on laborious tests carried out by incubation in different organic solvents and determination of residual activity. Here, we describe a simple assay useful for screening large libraries of Carboxylic Ester Hydrolases for resistance and activity in water-miscible organic solvents. We have screened a set of 26 enzymes, most of them identified in this study, with four different water-miscible organic solvents. The triglyceride tributyrin was used as a substrate, and fatty acids released by enzymatic hydrolysis were detected by a pH shift indicated by the indicator dye nitrazine yellow. With this strategy, we succeeded in identifying a novel highly organic-solvent-tolerant Esterase from Pseudomonas aestusnigri In addition, the newly identified enzymes were tested with sterically demanding substrates, which are common in pharmaceutical intermediates, and two enzymes from Alcanivorax borkumensis were identified which outcompeted the gold standard Ester hydrolase CalB from Candida antarctica IMPORTANCE Major challenges hampering biotechnological applications of Esterases include the requirement to accept nonnatural and chemically demanding substrates and the tolerance of the enzymes toward organic solvents which are often required to solubilize such substrates. We describe here a high-throughput screening strategy to identify novel organic-solvent-tolerant Carboxylic Ester Hydrolases (CEs). Among these enzymes, CEs active against water-insoluble bulky substrates were identified. Our results thus contribute to fostering the identification and biotechnological application of CEs.

  • Identification of organic solvent tolerant Carboxylic Ester Hydrolases for organic synthesis.
    Applied and Environmental Microbiology, 2020
    Co-Authors: Alexander Bollinger, Rebecka Molitor, Stephan Thies, Rainhard Koch, Cristina Coscolin, Manuel Ferrer, Karlerich Jaeger
    Abstract:

    Biocatalysis has emerged as an important tool in synthetic organic chemistry enabling the chemical industry to execute reactions with high regio- or enantioselectivity and under usually mild reaction conditions while avoiding toxic waste. Target substrates and products of reactions catalyzed by Carboxylic Ester Hydrolases are often poorly water-soluble and require organic solvents, enzymes are evolved by nature to be active in cells, i.e. in aqueous rather than organic solvents. Therefore, biocatalysts withstanding organic solvents are urgently needed. Current strategies to identify such enzymes rely on laborious tests carried out by incubation in different organic solvents and determination of residual activities. Here, we describe a simple assay useful to screen large libraries of Carboxylic Ester Hydrolases for resistance and activity in water miscible organic solvents. We have screened a set of 26 enzymes, most of them identified in this study, with four different water miscible organic solvents. The triglyceride tributyrin was used as a substrate and fatty acids released by enzymatic hydrolysis were detected by a pH shift indicated by the indicator dye nitrazine yellow. With this strategy, we succeeded to identify a novel highly organic solvent tolerant Esterase from Pseudomonas aestusnigri. In addition, the newly identified enzymes were tested with sterically demanding substrates, as common in pharmaceutical intermediates, and two enzymes from Alcanivorax borkumensis were identified which outcompeted the gold standard Ester hydrolase CalB from Candida antarctica. Importance Major challenges hampering biotechnological applications of Esterases include the requirement to accept non-natural and chemically demanding substrates and tolerance of the enzymes towards organic solvents which are often required to solubilize such substrates. We describe here a high throughput screening strategy to identify novel organic solvent tolerant Carboxylic Ester Hydrolases (CEs). Among these enzymes, CEs active against water-insoluble bulky substrates were identified. Our results thus contribute to fostering the identification and biotechnological application of CEs.

Manuel Ferrer - One of the best experts on this subject based on the ideXlab platform.

  • identification of organic solvent tolerant Carboxylic Ester Hydrolases for organic synthesis
    Applied and Environmental Microbiology, 2020
    Co-Authors: Alexander Bollinger, Rebecka Molitor, Stephan Thies, Rainhard Koch, Cristina Coscolin, Manuel Ferrer, Karlerich Jaeger
    Abstract:

    Biocatalysis has emerged as an important tool in synthetic organic chemistry enabling the chemical industry to execute reactions with high regio- or enantioselectivity and under usually mild reaction conditions while avoiding toxic waste. Target substrates and products of reactions catalyzed by Carboxylic Ester Hydrolases are often poorly water soluble and require organic solvents, whereas enzymes are evolved by nature to be active in cells, i.e., in aqueous rather than organic solvents. Therefore, biocatalysts that withstand organic solvents are urgently needed. Current strategies to identify such enzymes rely on laborious tests carried out by incubation in different organic solvents and determination of residual activity. Here, we describe a simple assay useful for screening large libraries of Carboxylic Ester Hydrolases for resistance and activity in water-miscible organic solvents. We have screened a set of 26 enzymes, most of them identified in this study, with four different water-miscible organic solvents. The triglyceride tributyrin was used as a substrate, and fatty acids released by enzymatic hydrolysis were detected by a pH shift indicated by the indicator dye nitrazine yellow. With this strategy, we succeeded in identifying a novel highly organic-solvent-tolerant Esterase from Pseudomonas aestusnigri In addition, the newly identified enzymes were tested with sterically demanding substrates, which are common in pharmaceutical intermediates, and two enzymes from Alcanivorax borkumensis were identified which outcompeted the gold standard Ester hydrolase CalB from Candida antarctica IMPORTANCE Major challenges hampering biotechnological applications of Esterases include the requirement to accept nonnatural and chemically demanding substrates and the tolerance of the enzymes toward organic solvents which are often required to solubilize such substrates. We describe here a high-throughput screening strategy to identify novel organic-solvent-tolerant Carboxylic Ester Hydrolases (CEs). Among these enzymes, CEs active against water-insoluble bulky substrates were identified. Our results thus contribute to fostering the identification and biotechnological application of CEs.

  • Identification of organic solvent tolerant Carboxylic Ester Hydrolases for organic synthesis.
    Applied and Environmental Microbiology, 2020
    Co-Authors: Alexander Bollinger, Rebecka Molitor, Stephan Thies, Rainhard Koch, Cristina Coscolin, Manuel Ferrer, Karlerich Jaeger
    Abstract:

    Biocatalysis has emerged as an important tool in synthetic organic chemistry enabling the chemical industry to execute reactions with high regio- or enantioselectivity and under usually mild reaction conditions while avoiding toxic waste. Target substrates and products of reactions catalyzed by Carboxylic Ester Hydrolases are often poorly water-soluble and require organic solvents, enzymes are evolved by nature to be active in cells, i.e. in aqueous rather than organic solvents. Therefore, biocatalysts withstanding organic solvents are urgently needed. Current strategies to identify such enzymes rely on laborious tests carried out by incubation in different organic solvents and determination of residual activities. Here, we describe a simple assay useful to screen large libraries of Carboxylic Ester Hydrolases for resistance and activity in water miscible organic solvents. We have screened a set of 26 enzymes, most of them identified in this study, with four different water miscible organic solvents. The triglyceride tributyrin was used as a substrate and fatty acids released by enzymatic hydrolysis were detected by a pH shift indicated by the indicator dye nitrazine yellow. With this strategy, we succeeded to identify a novel highly organic solvent tolerant Esterase from Pseudomonas aestusnigri. In addition, the newly identified enzymes were tested with sterically demanding substrates, as common in pharmaceutical intermediates, and two enzymes from Alcanivorax borkumensis were identified which outcompeted the gold standard Ester hydrolase CalB from Candida antarctica. Importance Major challenges hampering biotechnological applications of Esterases include the requirement to accept non-natural and chemically demanding substrates and tolerance of the enzymes towards organic solvents which are often required to solubilize such substrates. We describe here a high throughput screening strategy to identify novel organic solvent tolerant Carboxylic Ester Hydrolases (CEs). Among these enzymes, CEs active against water-insoluble bulky substrates were identified. Our results thus contribute to fostering the identification and biotechnological application of CEs.

Cristina Coscolin - One of the best experts on this subject based on the ideXlab platform.

  • identification of organic solvent tolerant Carboxylic Ester Hydrolases for organic synthesis
    Applied and Environmental Microbiology, 2020
    Co-Authors: Alexander Bollinger, Rebecka Molitor, Stephan Thies, Rainhard Koch, Cristina Coscolin, Manuel Ferrer, Karlerich Jaeger
    Abstract:

    Biocatalysis has emerged as an important tool in synthetic organic chemistry enabling the chemical industry to execute reactions with high regio- or enantioselectivity and under usually mild reaction conditions while avoiding toxic waste. Target substrates and products of reactions catalyzed by Carboxylic Ester Hydrolases are often poorly water soluble and require organic solvents, whereas enzymes are evolved by nature to be active in cells, i.e., in aqueous rather than organic solvents. Therefore, biocatalysts that withstand organic solvents are urgently needed. Current strategies to identify such enzymes rely on laborious tests carried out by incubation in different organic solvents and determination of residual activity. Here, we describe a simple assay useful for screening large libraries of Carboxylic Ester Hydrolases for resistance and activity in water-miscible organic solvents. We have screened a set of 26 enzymes, most of them identified in this study, with four different water-miscible organic solvents. The triglyceride tributyrin was used as a substrate, and fatty acids released by enzymatic hydrolysis were detected by a pH shift indicated by the indicator dye nitrazine yellow. With this strategy, we succeeded in identifying a novel highly organic-solvent-tolerant Esterase from Pseudomonas aestusnigri In addition, the newly identified enzymes were tested with sterically demanding substrates, which are common in pharmaceutical intermediates, and two enzymes from Alcanivorax borkumensis were identified which outcompeted the gold standard Ester hydrolase CalB from Candida antarctica IMPORTANCE Major challenges hampering biotechnological applications of Esterases include the requirement to accept nonnatural and chemically demanding substrates and the tolerance of the enzymes toward organic solvents which are often required to solubilize such substrates. We describe here a high-throughput screening strategy to identify novel organic-solvent-tolerant Carboxylic Ester Hydrolases (CEs). Among these enzymes, CEs active against water-insoluble bulky substrates were identified. Our results thus contribute to fostering the identification and biotechnological application of CEs.

  • Identification of organic solvent tolerant Carboxylic Ester Hydrolases for organic synthesis.
    Applied and Environmental Microbiology, 2020
    Co-Authors: Alexander Bollinger, Rebecka Molitor, Stephan Thies, Rainhard Koch, Cristina Coscolin, Manuel Ferrer, Karlerich Jaeger
    Abstract:

    Biocatalysis has emerged as an important tool in synthetic organic chemistry enabling the chemical industry to execute reactions with high regio- or enantioselectivity and under usually mild reaction conditions while avoiding toxic waste. Target substrates and products of reactions catalyzed by Carboxylic Ester Hydrolases are often poorly water-soluble and require organic solvents, enzymes are evolved by nature to be active in cells, i.e. in aqueous rather than organic solvents. Therefore, biocatalysts withstanding organic solvents are urgently needed. Current strategies to identify such enzymes rely on laborious tests carried out by incubation in different organic solvents and determination of residual activities. Here, we describe a simple assay useful to screen large libraries of Carboxylic Ester Hydrolases for resistance and activity in water miscible organic solvents. We have screened a set of 26 enzymes, most of them identified in this study, with four different water miscible organic solvents. The triglyceride tributyrin was used as a substrate and fatty acids released by enzymatic hydrolysis were detected by a pH shift indicated by the indicator dye nitrazine yellow. With this strategy, we succeeded to identify a novel highly organic solvent tolerant Esterase from Pseudomonas aestusnigri. In addition, the newly identified enzymes were tested with sterically demanding substrates, as common in pharmaceutical intermediates, and two enzymes from Alcanivorax borkumensis were identified which outcompeted the gold standard Ester hydrolase CalB from Candida antarctica. Importance Major challenges hampering biotechnological applications of Esterases include the requirement to accept non-natural and chemically demanding substrates and tolerance of the enzymes towards organic solvents which are often required to solubilize such substrates. We describe here a high throughput screening strategy to identify novel organic solvent tolerant Carboxylic Ester Hydrolases (CEs). Among these enzymes, CEs active against water-insoluble bulky substrates were identified. Our results thus contribute to fostering the identification and biotechnological application of CEs.

Rainhard Koch - One of the best experts on this subject based on the ideXlab platform.

  • identification of organic solvent tolerant Carboxylic Ester Hydrolases for organic synthesis
    Applied and Environmental Microbiology, 2020
    Co-Authors: Alexander Bollinger, Rebecka Molitor, Stephan Thies, Rainhard Koch, Cristina Coscolin, Manuel Ferrer, Karlerich Jaeger
    Abstract:

    Biocatalysis has emerged as an important tool in synthetic organic chemistry enabling the chemical industry to execute reactions with high regio- or enantioselectivity and under usually mild reaction conditions while avoiding toxic waste. Target substrates and products of reactions catalyzed by Carboxylic Ester Hydrolases are often poorly water soluble and require organic solvents, whereas enzymes are evolved by nature to be active in cells, i.e., in aqueous rather than organic solvents. Therefore, biocatalysts that withstand organic solvents are urgently needed. Current strategies to identify such enzymes rely on laborious tests carried out by incubation in different organic solvents and determination of residual activity. Here, we describe a simple assay useful for screening large libraries of Carboxylic Ester Hydrolases for resistance and activity in water-miscible organic solvents. We have screened a set of 26 enzymes, most of them identified in this study, with four different water-miscible organic solvents. The triglyceride tributyrin was used as a substrate, and fatty acids released by enzymatic hydrolysis were detected by a pH shift indicated by the indicator dye nitrazine yellow. With this strategy, we succeeded in identifying a novel highly organic-solvent-tolerant Esterase from Pseudomonas aestusnigri In addition, the newly identified enzymes were tested with sterically demanding substrates, which are common in pharmaceutical intermediates, and two enzymes from Alcanivorax borkumensis were identified which outcompeted the gold standard Ester hydrolase CalB from Candida antarctica IMPORTANCE Major challenges hampering biotechnological applications of Esterases include the requirement to accept nonnatural and chemically demanding substrates and the tolerance of the enzymes toward organic solvents which are often required to solubilize such substrates. We describe here a high-throughput screening strategy to identify novel organic-solvent-tolerant Carboxylic Ester Hydrolases (CEs). Among these enzymes, CEs active against water-insoluble bulky substrates were identified. Our results thus contribute to fostering the identification and biotechnological application of CEs.

  • Identification of organic solvent tolerant Carboxylic Ester Hydrolases for organic synthesis.
    Applied and Environmental Microbiology, 2020
    Co-Authors: Alexander Bollinger, Rebecka Molitor, Stephan Thies, Rainhard Koch, Cristina Coscolin, Manuel Ferrer, Karlerich Jaeger
    Abstract:

    Biocatalysis has emerged as an important tool in synthetic organic chemistry enabling the chemical industry to execute reactions with high regio- or enantioselectivity and under usually mild reaction conditions while avoiding toxic waste. Target substrates and products of reactions catalyzed by Carboxylic Ester Hydrolases are often poorly water-soluble and require organic solvents, enzymes are evolved by nature to be active in cells, i.e. in aqueous rather than organic solvents. Therefore, biocatalysts withstanding organic solvents are urgently needed. Current strategies to identify such enzymes rely on laborious tests carried out by incubation in different organic solvents and determination of residual activities. Here, we describe a simple assay useful to screen large libraries of Carboxylic Ester Hydrolases for resistance and activity in water miscible organic solvents. We have screened a set of 26 enzymes, most of them identified in this study, with four different water miscible organic solvents. The triglyceride tributyrin was used as a substrate and fatty acids released by enzymatic hydrolysis were detected by a pH shift indicated by the indicator dye nitrazine yellow. With this strategy, we succeeded to identify a novel highly organic solvent tolerant Esterase from Pseudomonas aestusnigri. In addition, the newly identified enzymes were tested with sterically demanding substrates, as common in pharmaceutical intermediates, and two enzymes from Alcanivorax borkumensis were identified which outcompeted the gold standard Ester hydrolase CalB from Candida antarctica. Importance Major challenges hampering biotechnological applications of Esterases include the requirement to accept non-natural and chemically demanding substrates and tolerance of the enzymes towards organic solvents which are often required to solubilize such substrates. We describe here a high throughput screening strategy to identify novel organic solvent tolerant Carboxylic Ester Hydrolases (CEs). Among these enzymes, CEs active against water-insoluble bulky substrates were identified. Our results thus contribute to fostering the identification and biotechnological application of CEs.