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Baoqin Han - One of the best experts on this subject based on the ideXlab platform.

  • Preparation and renoprotective effects of Carboxymethyl Chitosan oligosaccharide on adriamycin nephropathy.
    Carbohydrate polymers, 2018
    Co-Authors: Jing Qiao, Wanshun Liu, Zhiwen Jiang, Yuying Liu, Yan Yang, Baoqin Han
    Abstract:

    Abstract Carboxymethyl Chitosan oligosaccharide (CMCOS), the hydrolytic product of Carboxymethyl Chitosan, is nontoxic, easily absorbable and good antioxidant. In this study, CMCOS was prepared and its properties in adriamycin nephropathy therapy were investigated. Our results showed that CMCOS had good curative effects on renal function and parenchymal injury induced by adriamycin. CMCOS administration significantly relieved symptoms of proteinuria, hypoalbuminemia, hyperlipidemia, renal hyperplasia and histological lesions in rats (P

  • Evaluation on biological compatibility of Carboxymethyl Chitosan as biomaterials for antitumor drug delivery.
    Journal of biomaterials applications, 2017
    Co-Authors: Zhiwen Jiang, Baoqin Han, Wanshun Liu, Yanfei Peng
    Abstract:

    Carboxymethyl-Chitosan, a water-soluble derivative of Chitosan, has emerged as a promising candidate for biomedical applications due to its excellent water solubility, biodegradation, biocompatibility, hydrating, antimicrobial, and nontoxicity. In this paper, the antitumor proliferation and metastasis was studied in vitro and in vivo to evaluate biocompatibility of Carboxymethyl-Chitosan as biomaterials for antitumor drug delivery. The results showed that Carboxymethyl-Chitosan could significantly reduce the clone formation and tumor migration of human cancer cells including kidney cancer cell line OS-RC-2, gastric cancer cell line SGC-7901, colon cancer cell line HT-29, and nonsmall cell lung cancer cell line NCI-H1650 in vitro. Through Lewis tumor-bearing C57BL/6 mouse model, Carboxymethyl-Chitosan was proved to be able to inhibit solid tumor growth and tumor metastasis to the liver and lung, meanwhile increase the level of tissue inhibitor of metalloproteinase 1 and E-cadherin, and decrease the level of mice blood serum matrix metalloproteinase 9. This study suggested that Carboxymethyl-Chitosan had certain antimetastasis effect and good biocompatibility and may have a potential application as a synergic antitumor reagent.

  • Analgesis and Wound Healing Effect of Chitosan and Carboxymethyl Chitosan on Scalded Rats
    Journal of Ocean University of China, 2014
    Co-Authors: Shuya Huang, Baoqin Han, Kai Shao, Wanshun Liu
    Abstract:

    Analgesis and wound healing effect of Chitosan and Carboxymethyl Chitosan on scalded rats were investigated. A II degree scald model was established in rats, which was subsequently treated with Chitosan and Carboxymethyl Chitosan solution, respectively. The concentration of bradykinin and 5-hydroxytryptophan was detected by assaying enzyme-linked immunosorbent. Healing condition was observed and pathological sections were made to determine the healing effect of Chitosan and Carboxymethyl Chitosan. Results showed that the concentration of bradykinin and 5-hydroxytryptophan peaked at the third hour post-wound in all groups, while the concentration of hydroxyproline peaked at the seventh day post-wound in both Chitosan and Carboxymethyl Chitosan group. The concentration of bradykinin and 5-hydroxytryptophan of Carboxymethyl Chitosan group was significantly lower than that of control (P 0.05). These findings indicated that Carboxymethyl Chitosan reduced the concentration of algogenic substances, resulting in analgesia. During the whole recovery process, the hydroxyproline concentration in Chitosan and Carboxymethyl Chitosan group on day 3 and 7 was significantly higher than that of control (P < 0.01); however the significance of such a highness decreased on day 14 (P < 0.05). These findings indicated that Chitosan and Carboxymethyl Chitosan accelerated tissue repair. Meanwhile, Chitosan performed better in healing than Carboxymethyl Chitosan in both decrustation and healing time. In conclusion, Carboxymethyl Chitosan showed significant analgesis and wound-healing promotion effect, but Chitosan only showed wound-healing promotion effect.

  • Effects of Carboxymethyl-Chitosan on wound healing in vivo and in vitro
    Journal of Ocean University of China, 2011
    Co-Authors: Sikai Peng, Baoqin Han, Wanshun Liu, Jing Chang, Xuan Zhi
    Abstract:

    Effects of Carboxymethyl-Chitosan with different molecular weights on wound healing were investigated in vivo and in vitro. A second degree burn model was performed in rats and the accelerative effects of Carboxymethyl-Chitosan on wound repair were observed. Contents of transforming growth factor (TGF)-β1, interleukin(IL)-6 and matrix metalloproteinase (MMP)-1 in wounds were determined by enzyme-linked immunosorbent assay (ELISA). In vitro study evaluated the influence of Carboxymethyl-Chitosan on cytokines secretion of fibroblasts and macrophages. In vivo results showed that Carboxymethyl-Chitosan effectively accelerated the wound healing process in burned rats (P

  • The evaluation on biological properties of Carboxymethyl-Chitosan and Carboxymethyl-chitin
    Journal of Ocean University of China, 2008
    Co-Authors: Jing Chang, Baoqin Han, Wanshun Liu, Bing Liu
    Abstract:

    Carboxymethyl-Chitosan and Carboxymethyl-chitin were prepared with the methods developed in our laboratory. The DS (degree of substitution) and DD (degree of deacetylation) of the Carboxymethyl-Chitosan were 103.14% and 97.18% respectively, while the DS of the Carboxymethyl-chitin was 96.37%. Their effects on human fibroblasts, intradermal irritation test, in vitro and vivo degradability, and biocompatibility were evaluated. The results indicate that the polysaccharides at low concentrations can facilitate the growth of human fibroblasts and the Carboxymethyl-Chitosan at 100 μg mL−1 is the most effective. The polysaccharides at higher concentrations, however, inhibit the growth of fibroblasts. The PII (Primary Irritation Index) values of CM-Chitosan and CM-chitin are both 0.0, which shows that they have no irritation reaction. Both of the polysaccharides show good degradability and biocompatibility. Carboxymethyl-chitin degrades faster in vitro than Carboxymethyl-Chitosan. The latter, however, has no inflammatory reaction after being implanted in vivo for 7 d and shows better biocompatibility. This study may provide a scientific basis for the use of Carboxymethyl-Chitosan and Carboxymethyl-chitin as biomaterials.

Wanshun Liu - One of the best experts on this subject based on the ideXlab platform.

  • Preparation and renoprotective effects of Carboxymethyl Chitosan oligosaccharide on adriamycin nephropathy.
    Carbohydrate polymers, 2018
    Co-Authors: Jing Qiao, Wanshun Liu, Zhiwen Jiang, Yuying Liu, Yan Yang, Baoqin Han
    Abstract:

    Abstract Carboxymethyl Chitosan oligosaccharide (CMCOS), the hydrolytic product of Carboxymethyl Chitosan, is nontoxic, easily absorbable and good antioxidant. In this study, CMCOS was prepared and its properties in adriamycin nephropathy therapy were investigated. Our results showed that CMCOS had good curative effects on renal function and parenchymal injury induced by adriamycin. CMCOS administration significantly relieved symptoms of proteinuria, hypoalbuminemia, hyperlipidemia, renal hyperplasia and histological lesions in rats (P

  • Evaluation on biological compatibility of Carboxymethyl Chitosan as biomaterials for antitumor drug delivery.
    Journal of biomaterials applications, 2017
    Co-Authors: Zhiwen Jiang, Baoqin Han, Wanshun Liu, Yanfei Peng
    Abstract:

    Carboxymethyl-Chitosan, a water-soluble derivative of Chitosan, has emerged as a promising candidate for biomedical applications due to its excellent water solubility, biodegradation, biocompatibility, hydrating, antimicrobial, and nontoxicity. In this paper, the antitumor proliferation and metastasis was studied in vitro and in vivo to evaluate biocompatibility of Carboxymethyl-Chitosan as biomaterials for antitumor drug delivery. The results showed that Carboxymethyl-Chitosan could significantly reduce the clone formation and tumor migration of human cancer cells including kidney cancer cell line OS-RC-2, gastric cancer cell line SGC-7901, colon cancer cell line HT-29, and nonsmall cell lung cancer cell line NCI-H1650 in vitro. Through Lewis tumor-bearing C57BL/6 mouse model, Carboxymethyl-Chitosan was proved to be able to inhibit solid tumor growth and tumor metastasis to the liver and lung, meanwhile increase the level of tissue inhibitor of metalloproteinase 1 and E-cadherin, and decrease the level of mice blood serum matrix metalloproteinase 9. This study suggested that Carboxymethyl-Chitosan had certain antimetastasis effect and good biocompatibility and may have a potential application as a synergic antitumor reagent.

  • Analgesis and Wound Healing Effect of Chitosan and Carboxymethyl Chitosan on Scalded Rats
    Journal of Ocean University of China, 2014
    Co-Authors: Shuya Huang, Baoqin Han, Kai Shao, Wanshun Liu
    Abstract:

    Analgesis and wound healing effect of Chitosan and Carboxymethyl Chitosan on scalded rats were investigated. A II degree scald model was established in rats, which was subsequently treated with Chitosan and Carboxymethyl Chitosan solution, respectively. The concentration of bradykinin and 5-hydroxytryptophan was detected by assaying enzyme-linked immunosorbent. Healing condition was observed and pathological sections were made to determine the healing effect of Chitosan and Carboxymethyl Chitosan. Results showed that the concentration of bradykinin and 5-hydroxytryptophan peaked at the third hour post-wound in all groups, while the concentration of hydroxyproline peaked at the seventh day post-wound in both Chitosan and Carboxymethyl Chitosan group. The concentration of bradykinin and 5-hydroxytryptophan of Carboxymethyl Chitosan group was significantly lower than that of control (P 0.05). These findings indicated that Carboxymethyl Chitosan reduced the concentration of algogenic substances, resulting in analgesia. During the whole recovery process, the hydroxyproline concentration in Chitosan and Carboxymethyl Chitosan group on day 3 and 7 was significantly higher than that of control (P < 0.01); however the significance of such a highness decreased on day 14 (P < 0.05). These findings indicated that Chitosan and Carboxymethyl Chitosan accelerated tissue repair. Meanwhile, Chitosan performed better in healing than Carboxymethyl Chitosan in both decrustation and healing time. In conclusion, Carboxymethyl Chitosan showed significant analgesis and wound-healing promotion effect, but Chitosan only showed wound-healing promotion effect.

  • Effects of Carboxymethyl-Chitosan on wound healing in vivo and in vitro
    Journal of Ocean University of China, 2011
    Co-Authors: Sikai Peng, Baoqin Han, Wanshun Liu, Jing Chang, Xuan Zhi
    Abstract:

    Effects of Carboxymethyl-Chitosan with different molecular weights on wound healing were investigated in vivo and in vitro. A second degree burn model was performed in rats and the accelerative effects of Carboxymethyl-Chitosan on wound repair were observed. Contents of transforming growth factor (TGF)-β1, interleukin(IL)-6 and matrix metalloproteinase (MMP)-1 in wounds were determined by enzyme-linked immunosorbent assay (ELISA). In vitro study evaluated the influence of Carboxymethyl-Chitosan on cytokines secretion of fibroblasts and macrophages. In vivo results showed that Carboxymethyl-Chitosan effectively accelerated the wound healing process in burned rats (P

  • The evaluation on biological properties of Carboxymethyl-Chitosan and Carboxymethyl-chitin
    Journal of Ocean University of China, 2008
    Co-Authors: Jing Chang, Baoqin Han, Wanshun Liu, Bing Liu
    Abstract:

    Carboxymethyl-Chitosan and Carboxymethyl-chitin were prepared with the methods developed in our laboratory. The DS (degree of substitution) and DD (degree of deacetylation) of the Carboxymethyl-Chitosan were 103.14% and 97.18% respectively, while the DS of the Carboxymethyl-chitin was 96.37%. Their effects on human fibroblasts, intradermal irritation test, in vitro and vivo degradability, and biocompatibility were evaluated. The results indicate that the polysaccharides at low concentrations can facilitate the growth of human fibroblasts and the Carboxymethyl-Chitosan at 100 μg mL−1 is the most effective. The polysaccharides at higher concentrations, however, inhibit the growth of fibroblasts. The PII (Primary Irritation Index) values of CM-Chitosan and CM-chitin are both 0.0, which shows that they have no irritation reaction. Both of the polysaccharides show good degradability and biocompatibility. Carboxymethyl-chitin degrades faster in vitro than Carboxymethyl-Chitosan. The latter, however, has no inflammatory reaction after being implanted in vivo for 7 d and shows better biocompatibility. This study may provide a scientific basis for the use of Carboxymethyl-Chitosan and Carboxymethyl-chitin as biomaterials.

Zhanyong Guo - One of the best experts on this subject based on the ideXlab platform.

  • Antifungal Activity of Quaternized Carboxymethyl Chitosan
    2008 2nd International Conference on Bioinformatics and Biomedical Engineering, 2008
    Co-Authors: Zhanyong Guo, Ronge Xing, Song Liu, Li Pengcheng
    Abstract:

    Quaternized Carboxymethyl Chitosan (QCMC) were synthesized and their antifungal activities against Alternaria Solani (A. Solani) and Physalospora piricola Nose (P. piricola Nose) were investigated. The results indicated that the quaternized Carboxymethyl Chitosan derivatives had better inhibitory effects than CMC, and the antifungal activities should be affected by the cation in these compounds.

  • Synthesis and hydroxyl radicals scavenging activity of quaternized Carboxymethyl Chitosan
    Carbohydrate Polymers, 2008
    Co-Authors: Zhanyong Guo, Ronge Xing, Song Liu, Zhimei Zhong
    Abstract:

    In order to determine the effect of the forms of the amido groups of Chitosan on antioxidant activity, quaternized Carboxymethyl Chitosan (QCMC) derivatives were prepared with a degree of quaternization ranging from 34.3% to 59.5%. The antioxidant activity of QCMCs against hydroxyl radicals was assessed. The results indicated that QCMCs have better hydroxyl radicals scavenging activity than that of Carboxymethyl Chitosan, as a result of the positive charge of the quaternized Chitosan. (C) 2007 Elsevier Ltd. All rights reserved.

Zhimei Zhong - One of the best experts on this subject based on the ideXlab platform.

  • Synthesis and hydroxyl radicals scavenging activity of quaternized Carboxymethyl Chitosan
    Carbohydrate Polymers, 2008
    Co-Authors: Zhanyong Guo, Ronge Xing, Song Liu, Zhimei Zhong
    Abstract:

    In order to determine the effect of the forms of the amido groups of Chitosan on antioxidant activity, quaternized Carboxymethyl Chitosan (QCMC) derivatives were prepared with a degree of quaternization ranging from 34.3% to 59.5%. The antioxidant activity of QCMCs against hydroxyl radicals was assessed. The results indicated that QCMCs have better hydroxyl radicals scavenging activity than that of Carboxymethyl Chitosan, as a result of the positive charge of the quaternized Chitosan. (C) 2007 Elsevier Ltd. All rights reserved.

Song Liu - One of the best experts on this subject based on the ideXlab platform.

  • Antifungal Activity of Quaternized Carboxymethyl Chitosan
    2008 2nd International Conference on Bioinformatics and Biomedical Engineering, 2008
    Co-Authors: Zhanyong Guo, Ronge Xing, Song Liu, Li Pengcheng
    Abstract:

    Quaternized Carboxymethyl Chitosan (QCMC) were synthesized and their antifungal activities against Alternaria Solani (A. Solani) and Physalospora piricola Nose (P. piricola Nose) were investigated. The results indicated that the quaternized Carboxymethyl Chitosan derivatives had better inhibitory effects than CMC, and the antifungal activities should be affected by the cation in these compounds.

  • Synthesis and hydroxyl radicals scavenging activity of quaternized Carboxymethyl Chitosan
    Carbohydrate Polymers, 2008
    Co-Authors: Zhanyong Guo, Ronge Xing, Song Liu, Zhimei Zhong
    Abstract:

    In order to determine the effect of the forms of the amido groups of Chitosan on antioxidant activity, quaternized Carboxymethyl Chitosan (QCMC) derivatives were prepared with a degree of quaternization ranging from 34.3% to 59.5%. The antioxidant activity of QCMCs against hydroxyl radicals was assessed. The results indicated that QCMCs have better hydroxyl radicals scavenging activity than that of Carboxymethyl Chitosan, as a result of the positive charge of the quaternized Chitosan. (C) 2007 Elsevier Ltd. All rights reserved.