The Experts below are selected from a list of 1197 Experts worldwide ranked by ideXlab platform
Jun Zhang - One of the best experts on this subject based on the ideXlab platform.
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protective role of matrine in sepsis associated Cardiac dysfunction through regulating the lncrna ptenp1 mir 106b 5p axis
Biomedicine & Pharmacotherapy, 2021Co-Authors: Yujuan Liu, Lijun Liu, Jun ZhangAbstract:Abstract Background Matrine has attractive cardioprotective effects in some diseases. This study aimed to evaluate the therapeutic potential of matrine against Cardiac dysfunction induced by sepsis in vivo and in vitro, and further explore the related mechanisms. Methods Cecal ligation and puncture (CLP) was used to induce a sepsis mice model, and H9C2 cells treated with lipopolysaccharide (LPS) were used as a Cardiac Myoblast injury model. The evaluation of Cardiac function of mice was performed by measuring Cardiac function biomarker levels and hemodynamic indicators. An ELISA method was used to examine inflammatory cytokine levels. H9C2 cell viability was measured using MTT assay. The expression of non-coding RNAs that might be involved in matrine function was analyzed using real-time quantitative PCR. Results Matrine could significantly improve the Cardiac function and attenuate the inflammatory response of the mice model, and could increase H9C2 viability and inhibit inflammation in the cell model. By matrine administration, the expression of PTENP1 was downregulated, but miR-106b-5p expression was upregulated both in vivo and in vitro. The cardioprotective effects of matrine in mice and cell models could be reversed by the overexpression of PTENP1 or the knockdown of miR-106b-5p, and the overexpression of miR-106b-5p could significantly abolish the effects of PTENP1 on Cardiac function and inflammation. Conclusion All the data revealed that matrine can alleviate sepsis-related Cardiac dysfunction by enhancing Cardiac Myoblast viability and attenuating inflammatory responses through the PTENP1/miR-106b-5p axis.
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Protective role of matrine in sepsis-associated Cardiac dysfunction through regulating the lncRNA PTENP1/miR-106b-5p axis.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2020Co-Authors: Yujuan Liu, Lijun Liu, Jun ZhangAbstract:Abstract Background Matrine has attractive cardioprotective effects in some diseases. This study aimed to evaluate the therapeutic potential of matrine against Cardiac dysfunction induced by sepsis in vivo and in vitro, and further explore the related mechanisms. Methods Cecal ligation and puncture (CLP) was used to induce a sepsis mice model, and H9C2 cells treated with lipopolysaccharide (LPS) were used as a Cardiac Myoblast injury model. The evaluation of Cardiac function of mice was performed by measuring Cardiac function biomarker levels and hemodynamic indicators. An ELISA method was used to examine inflammatory cytokine levels. H9C2 cell viability was measured using MTT assay. The expression of non-coding RNAs that might be involved in matrine function was analyzed using real-time quantitative PCR. Results Matrine could significantly improve the Cardiac function and attenuate the inflammatory response of the mice model, and could increase H9C2 viability and inhibit inflammation in the cell model. By matrine administration, the expression of PTENP1 was downregulated, but miR-106b-5p expression was upregulated both in vivo and in vitro. The cardioprotective effects of matrine in mice and cell models could be reversed by the overexpression of PTENP1 or the knockdown of miR-106b-5p, and the overexpression of miR-106b-5p could significantly abolish the effects of PTENP1 on Cardiac function and inflammation. Conclusion All the data revealed that matrine can alleviate sepsis-related Cardiac dysfunction by enhancing Cardiac Myoblast viability and attenuating inflammatory responses through the PTENP1/miR-106b-5p axis.
Helder A Santos - One of the best experts on this subject based on the ideXlab platform.
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fabrication and characterization of drug loaded conductive poly glycerol sebacate nanoparticle based composite patch for myocardial infarction applications
ACS Applied Materials & Interfaces, 2020Co-Authors: Nazanin Zanjanizadeh Ezazi, Rubina Ajdary, Alexandra Correia, Ermei Makila, Jarno Salonen, Marianna Kemell, Jouni Hirvonen, Orlando J Rojas, Heikki Ruskoaho, Helder A SantosAbstract:Heart tissue engineering is critical in the treatment of myocardial infarction, which may benefit from drug-releasing smart materials. In this study, we load a small molecule (3i-1000) in new biodegradable and conductive patches for application in infarcted myocardium. The composite patches consist of a biocompatible elastomer, poly(glycerol sebacate) (PGS), coupled with collagen type I, used to promote cell attachment. In addition, polypyrrole is incorporated because of its electrical conductivity and to induce cell signaling. Results from the in vitro experiments indicate a high density of Cardiac Myoblast cells attached on the patches, which stay viable for at least 1 month. The degradation of the patches does not show any cytotoxic effect, while 3i-1000 delivery induces cell proliferation. Conductive patches show high blood wettability and drug release, correlating with the rate of degradation of the PGS matrix. Together with the electrical conductivity and elongation characteristics, the developed biomaterial fits the mechanical, conductive, and biological demands required for Cardiac treatment.
Yujuan Liu - One of the best experts on this subject based on the ideXlab platform.
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protective role of matrine in sepsis associated Cardiac dysfunction through regulating the lncrna ptenp1 mir 106b 5p axis
Biomedicine & Pharmacotherapy, 2021Co-Authors: Yujuan Liu, Lijun Liu, Jun ZhangAbstract:Abstract Background Matrine has attractive cardioprotective effects in some diseases. This study aimed to evaluate the therapeutic potential of matrine against Cardiac dysfunction induced by sepsis in vivo and in vitro, and further explore the related mechanisms. Methods Cecal ligation and puncture (CLP) was used to induce a sepsis mice model, and H9C2 cells treated with lipopolysaccharide (LPS) were used as a Cardiac Myoblast injury model. The evaluation of Cardiac function of mice was performed by measuring Cardiac function biomarker levels and hemodynamic indicators. An ELISA method was used to examine inflammatory cytokine levels. H9C2 cell viability was measured using MTT assay. The expression of non-coding RNAs that might be involved in matrine function was analyzed using real-time quantitative PCR. Results Matrine could significantly improve the Cardiac function and attenuate the inflammatory response of the mice model, and could increase H9C2 viability and inhibit inflammation in the cell model. By matrine administration, the expression of PTENP1 was downregulated, but miR-106b-5p expression was upregulated both in vivo and in vitro. The cardioprotective effects of matrine in mice and cell models could be reversed by the overexpression of PTENP1 or the knockdown of miR-106b-5p, and the overexpression of miR-106b-5p could significantly abolish the effects of PTENP1 on Cardiac function and inflammation. Conclusion All the data revealed that matrine can alleviate sepsis-related Cardiac dysfunction by enhancing Cardiac Myoblast viability and attenuating inflammatory responses through the PTENP1/miR-106b-5p axis.
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Protective role of matrine in sepsis-associated Cardiac dysfunction through regulating the lncRNA PTENP1/miR-106b-5p axis.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2020Co-Authors: Yujuan Liu, Lijun Liu, Jun ZhangAbstract:Abstract Background Matrine has attractive cardioprotective effects in some diseases. This study aimed to evaluate the therapeutic potential of matrine against Cardiac dysfunction induced by sepsis in vivo and in vitro, and further explore the related mechanisms. Methods Cecal ligation and puncture (CLP) was used to induce a sepsis mice model, and H9C2 cells treated with lipopolysaccharide (LPS) were used as a Cardiac Myoblast injury model. The evaluation of Cardiac function of mice was performed by measuring Cardiac function biomarker levels and hemodynamic indicators. An ELISA method was used to examine inflammatory cytokine levels. H9C2 cell viability was measured using MTT assay. The expression of non-coding RNAs that might be involved in matrine function was analyzed using real-time quantitative PCR. Results Matrine could significantly improve the Cardiac function and attenuate the inflammatory response of the mice model, and could increase H9C2 viability and inhibit inflammation in the cell model. By matrine administration, the expression of PTENP1 was downregulated, but miR-106b-5p expression was upregulated both in vivo and in vitro. The cardioprotective effects of matrine in mice and cell models could be reversed by the overexpression of PTENP1 or the knockdown of miR-106b-5p, and the overexpression of miR-106b-5p could significantly abolish the effects of PTENP1 on Cardiac function and inflammation. Conclusion All the data revealed that matrine can alleviate sepsis-related Cardiac dysfunction by enhancing Cardiac Myoblast viability and attenuating inflammatory responses through the PTENP1/miR-106b-5p axis.
Nazanin Zanjanizadeh Ezazi - One of the best experts on this subject based on the ideXlab platform.
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fabrication and characterization of drug loaded conductive poly glycerol sebacate nanoparticle based composite patch for myocardial infarction applications
ACS Applied Materials & Interfaces, 2020Co-Authors: Nazanin Zanjanizadeh Ezazi, Rubina Ajdary, Alexandra Correia, Ermei Makila, Jarno Salonen, Marianna Kemell, Jouni Hirvonen, Orlando J Rojas, Heikki Ruskoaho, Helder A SantosAbstract:Heart tissue engineering is critical in the treatment of myocardial infarction, which may benefit from drug-releasing smart materials. In this study, we load a small molecule (3i-1000) in new biodegradable and conductive patches for application in infarcted myocardium. The composite patches consist of a biocompatible elastomer, poly(glycerol sebacate) (PGS), coupled with collagen type I, used to promote cell attachment. In addition, polypyrrole is incorporated because of its electrical conductivity and to induce cell signaling. Results from the in vitro experiments indicate a high density of Cardiac Myoblast cells attached on the patches, which stay viable for at least 1 month. The degradation of the patches does not show any cytotoxic effect, while 3i-1000 delivery induces cell proliferation. Conductive patches show high blood wettability and drug release, correlating with the rate of degradation of the PGS matrix. Together with the electrical conductivity and elongation characteristics, the developed biomaterial fits the mechanical, conductive, and biological demands required for Cardiac treatment.
Varma S Penumathsa - One of the best experts on this subject based on the ideXlab platform.
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resveratrol enhances glut 4 translocation to the caveolar lipid raft fractions through ampk akt enos signalling pathway in diabetic myocardium
Journal of Cellular and Molecular Medicine, 2008Co-Authors: Varma S Penumathsa, Mahesh Thirunavukkarasu, Lijun Zhan, Gautam Maulik, Venogopal P Menon, Debasis Bagchi, Nilanjana MaulikAbstract:Homeostasis of blood glucose by insulin involves stimulation of glucose uptake by translocation of glucose transporter Glut-4 from intracellular pool to the caveolar membrane system. In this study we examined resveratrol (RSV)-mediated Glut-4 translocation in the streptozotocin (STZ)-induced diabetic myocardium. The rats were randomized into three groups: Control (Con), Diabetes Mellitus (DM) (STZ 65 mg/kg b.w., i.p.) & DM + RSV (2.5 mg/kg b.wt. for 2 weeks orally) (RSV). Isolated rat hearts were used as per the experimental model. RSV induced glucose uptake was observed in vitro with H9c2 Cardiac Myoblast cells. Decreased blood glucose level was observed after 30 days (375 mg/dl) in RSV-treated rats when compared to DM (587 mg/dl). Treatment with RSV demonstrated increased Adenosine Mono Phosphate Kinase (AMPK) phosphorylation compared to DM. Lipid raft fractions demonstrated decreased expression of Glut-4, Cav-3 (0.4, 0.6-fold) in DM which was increased to 0.75-and 1.1-fold on RSV treatment as compared to control. Increased Cav-1 expression (1.4-fold) in DM was reduced to 0.7-fold on RSV treatment. Increased phosphorylation of endothelial Nitric Oxide Synthase (eNOS) & Akt was also observed in RSV compared to DM (P< 0.05). Confocal microscopy and co-immunoprecipitation studies demonstrated decreased association of Glut-4/Cav-3 and increased association of Cav-1/eNOS in DM as compared to control and converse results were obtained on RSV treatment. Our results suggests that the effect of RSV is non-insulin dependent and triggers some of the similar intracellular insulin signalling components in myocardium such as eNOS, Akt through AMPK pathway and also by regulating the caveolin-1 and caveolin-3 status that might play an essential role in Glut-4 translocation and glucose uptake in STZ- induced type-1 diabetic myocardium.