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Yue Jin - One of the best experts on this subject based on the ideXlab platform.
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extracts and components of ficus Carica leaves suppress survival cell cycle and migration of triple negative breast cancer mda mb 231 cells
OncoTargets and Therapy, 2018Co-Authors: Yu Zhang, Youzhong Wan, Bo Huo, Yue JinAbstract:Background Products from Ficus Carica have been used in traditional medicine to treat many diseases. This study aimed to analyze anticancer effects of extracts of F. Carica leaves on the triple-negative breast cancer cell line MDA-MB-231. Materials and methods The human breast cancer cell line MDA-MB-231 was used to evaluate effects of F. Carica extracts. Effects of F. Carica on cell viability were evaluated using MTT assays. Cell-cycle distribution was examined using cell-cycle analysis. Wound-healing assays were used to evaluate migration of MDA-MB-231. Quantitative reverse-transcription polymerase chain reaction was used to detect levels of Bax, p53, p21, GATA3, ELF5, cyclin-dependent kinases, MMP2, and tissue inhibitors of metalloproteinase. Results We investigated the mechanism of anti-growth effects, and found that the expressions of genes that promote apoptosis were increased. In addition, the treated cells illustrated increased portion at S phase and changed expression of cyclin-dependent kinases, demonstrating cell-cycle arrest at the S phase. Furthermore, treated cells showed decreased cell mobility, which is essential for metastasis. Two of the active components of F. Carica leaves, bergapten and psoralen, had similar anticancer effects as F. Carica leaf extracts, indicating that these two components might play important roles in anticancer effects of F. Carica leaves. Conclusion Our findings suggest that F. Carica leaves might be a good source to develop drugs for suppressing cancer-cell growth and migration to treat triple-negative breast cancers.
Yu Zhang - One of the best experts on this subject based on the ideXlab platform.
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extracts and components of ficus Carica leaves suppress survival cell cycle and migration of triple negative breast cancer mda mb 231 cells
OncoTargets and Therapy, 2018Co-Authors: Yu Zhang, Youzhong Wan, Bo Huo, Yue JinAbstract:Background Products from Ficus Carica have been used in traditional medicine to treat many diseases. This study aimed to analyze anticancer effects of extracts of F. Carica leaves on the triple-negative breast cancer cell line MDA-MB-231. Materials and methods The human breast cancer cell line MDA-MB-231 was used to evaluate effects of F. Carica extracts. Effects of F. Carica on cell viability were evaluated using MTT assays. Cell-cycle distribution was examined using cell-cycle analysis. Wound-healing assays were used to evaluate migration of MDA-MB-231. Quantitative reverse-transcription polymerase chain reaction was used to detect levels of Bax, p53, p21, GATA3, ELF5, cyclin-dependent kinases, MMP2, and tissue inhibitors of metalloproteinase. Results We investigated the mechanism of anti-growth effects, and found that the expressions of genes that promote apoptosis were increased. In addition, the treated cells illustrated increased portion at S phase and changed expression of cyclin-dependent kinases, demonstrating cell-cycle arrest at the S phase. Furthermore, treated cells showed decreased cell mobility, which is essential for metastasis. Two of the active components of F. Carica leaves, bergapten and psoralen, had similar anticancer effects as F. Carica leaf extracts, indicating that these two components might play important roles in anticancer effects of F. Carica leaves. Conclusion Our findings suggest that F. Carica leaves might be a good source to develop drugs for suppressing cancer-cell growth and migration to treat triple-negative breast cancers.
Dewa Judhistira Guntara - One of the best experts on this subject based on the ideXlab platform.
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KONSENTRASI HAMBAT DAN BUNUH MINIMUM FORMULA HIDROGEL EKSTRAK DAUN TIN (FICUS Carica) TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS
Fakultas Kedokteran Gigi Universitas Mahasaraswati Denpasar, 2020Co-Authors: Dewa Judhistira GuntaraAbstract:Staphylococcus aureus merupakan patogen yang dapat menyebabkan berbagai infeksi sekunder seperti infeksi pada kulit dan jaringan lunak di rongga mulut, dilaporkan bahwa S. aureus memiliki peran dalam kegagalan implan gigi, menginfeksi perawatan saluran akar dan juga dapat berkolonisasi pada mukosa pengguna gigi tiruan. Penanganan akibat infeksi S. aureus biasanya menggunakan antibiotik, tetapi penggunaan antibiotik secara berlebihan akan menyebabkan perubahan ekologi dan menimbulkan resistensi di rongga mulut. Daun Tin (Ficus Carica) yang diekstrak dibuat dalam sedian hidrogel dan dapat dijadikan obat karena memiliki kandungan senyawa aktif seperti alkaloid, flavonoid, kumarin, saponin, polifenol dan tanin yang dapat menghambat dan membunuh bakteri dan berfungsi sebagai antibakteri. Tujuan penelitian ini untuk mengetahui Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) formula hidrogel ekstrak daun Tin (Ficus Carica) terhadap pertumbuhan Staphylococcus aureus. Daun Tin (Ficus Carica) diekstraksi dengan metode maserasi, selanjutnya dilakukan uji konsentrasi hambat dan bunuh minimum dengan metode dilusi. Berdasarkan hasil uji Kruskal-Wallis diperoleh nilai P = 0,63 (P>0,05) yang menunjukan tidak terdapat perbedaan yang bermakna antara kelompok perlakuan terhadap pertumbuhan S. aureus. Hasil perlakuan menunjukan nilai tertinggi sampai terendah yaitu kontrol negatif, 5%, 25%, 50%, 100% dan kontrol positif. Kesimpulan penelitian ini didapatkan konsentrasi formula hidrogel ekstrak daun Tin (Ficus Carica) dalam menghambat S. aureus adalah pada konsentrasi 5% dan Konsentrasi Bunuh Minimum formula hidrogel ekstrak daun Tin (Ficus Carica) pada ekstrak murni 100%.Banda Ace
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KONSENTRASI HAMBAT DAN BUNUH MINIMUM FORMULA HIDROGEL EKSTRAK DAUN TIN (FICUS Carica) TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS
Fakultas Kedokteran Gigi Universitas Mahasaraswati Denpasar, 2020Co-Authors: Dewa Judhistira GuntaraAbstract:Staphylococcus aureus merupakan patogen yang dapat menyebabkan berbagai infeksi sekunder seperti infeksi pada kulit dan jaringan lunak di rongga mulut, dilaporkan bahwa S. aureus memiliki peran dalam kegagalan implan gigi, menginfeksi perawatan saluran akar dan juga dapat berkolonisasi pada mukosa pengguna gigi tiruan. Penanganan akibat infeksi S. aureus biasanya menggunakan antibiotik, tetapi penggunaan antibiotik secara berlebihan akan menyebabkan perubahan ekologi dan menimbulkan resistensi di rongga mulut. Daun Tin (Ficus Carica) yang diekstrak dibuat dalam sedian hidrogel dan dapat dijadikan obat karena memiliki kandungan senyawa aktif seperti alkaloid, flavonoid, kumarin, saponin, polifenol dan tanin yang dapat menghambat dan membunuh bakteri dan berfungsi sebagai antibakteri. Tujuan penelitian ini untuk mengetahui Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) formula hidrogel ekstrak daun Tin (Ficus Carica) terhadap pertumbuhan Staphylococcus aureus. Daun Tin (Ficus Carica) diekstraksi dengan metode maserasi, selanjutnya dilakukan uji konsentrasi hambat dan bunuh minimum dengan metode dilusi. Berdasarkan hasil uji Kruskal-Wallis diperoleh nilai P = 0,63 (P>0,05) yang menunjukan tidak terdapat perbedaan yang bermakna antara kelompok perlakuan terhadap pertumbuhan S. aureus. Hasil perlakuan menunjukan nilai tertinggi sampai terendah yaitu kontrol negatif, 5%, 25%, 50%, 100% dan kontrol positif. Kesimpulan penelitian ini didapatkan konsentrasi formula hidrogel ekstrak daun Tin (Ficus Carica) dalam menghambat S. aureus adalah pada konsentrasi 5%, dan pada konsentrasi 100% tidak menunjukan hasil uji yang sama seperti kontrol positif.Kata Kunci : Staphylococcus aureus, Hidrogel, Daun Tin (Ficus Carica), Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM)Banda Ace
Rômulo Magno Oliveira De ,freitas - One of the best experts on this subject based on the ideXlab platform.
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Levantamento etnobotânico das espécies vegetais em quintais de bairro na cidade de Mossoró, Rio Grande do Norte
2016Co-Authors: Coelho, Maria De Fatima Barbosa, Pereira Leal, Caio César, Nascimento De Oliveira, Fabrícia, Nogueira, Narjara Walessa, Rômulo Magno Oliveira De ,freitasAbstract:Homegardens are known by different denominations in various places and can be defined as a production area located near the house, where agricultural and forestry species are grown, with the creation of small domestic animals. The present work had as objective to carry out an ethnobotanical survey of the vegetal species existing in the homegardens of the Alto Sumaré neighborhood in Mossoró, Rio Grande do Norte. The selection of fifteen informants was done using the snowball technique and the data collection was done with direct observation and participant, guided tour, and semi-structured interviews. It was observed 65 species belonging to 38 botanical families. The family with the highest number of species was Lamiaceae and the most frequent species were Carica papaya L. and Mangifera indica L. Alocasia macrorrhizos (L.) G. Don and Carica papaya L. were the most abundant species. Of those interviewed, 80% reported using medicinal plants for disease treatments and 24 species have medicinal uses. The predominant categories of use were food, medicinal and ornamental. Mentha X piperita L. and Lippia alba (Mill.) N. E. Br. Ex Britton & P. Wilson were the most cited as medicinal, Carica papaya and Mangifera indica as fruitful and Dieffenbachia picta Schott as ornamental.Os quintais são conhecidos por diferentes denominações em vários lugares e podem ser definidos como uma área de produção localizada perto da casa, onde são cultivadas espécies agrícolas e florestais, com a criação de pequenos animais domésticos. O presente trabalho teve como objetivo realizar um levantamento etnobotânico das espécies vegetais existentes nos quintais do bairro Alto Sumaré em Mossoró, Rio Grande do Norte. A seleção de quinze informantes foi feita usando a técnica de bola de neve e a coleta dos dados foi efetuada com observação direta e participante, turnê guiada, e entrevistas semiestruturadas. Foram observadas 65 espécies pertencentes a 38 famílias botânicas. A família com maior número de espécies foi Lamiaceae e as espécies mais frequentes foram Carica papaya L. e Mangifera indica L. Alocasia macrorrhizos (L.) G. Don e Carica papaya L. foram as espécies mais abundantes. Dos entrevistados, 80% declararam utilizar plantas medicinais para tratamentos de doenças e 24 espécies têm uso medicinal. As categorias de uso predominantes foram alimentícia, medicinal e ornamental. Mentha X piperita L. e Lippia alba (Mill.) N. E. Br. ex Britton & P. Wilson foram as mais citadas como medicinal, Carica papaya e Mangifera indica como frutífera e Dieffenbachia picta Schott como ornamental
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Levantamento etnobotânico das espécies vegetais em quintais de bairro na cidade de Mossoró, Rio Grande do Norte
Grupo Verde de Agroecologia e Abelhas (GVAA), 2016Co-Authors: Maria De Fatima Barbosa Coelho, Caio César Pereira Leal, Fabrícia Nascimento De Oliveira, Narjara Walessa Nogueira, Rômulo Magno Oliveira De ,freitasAbstract:Os quintais são conhecidos por diferentes denominações em vários lugares e podem ser definidos como uma área de produção localizada perto da casa, onde são cultivadas espécies agrícolas e florestais, com a criação de pequenos animais domésticos. O presente trabalho teve como objetivo realizar um levantamento etnobotânico das espécies vegetais existentes nos quintais do bairro Alto Sumaré em Mossoró, Rio Grande do Norte. A seleção de quinze informantes foi feita usando a técnica de bola de neve e a coleta dos dados foi efetuada com observação direta e participante, turnê guiada, e entrevistas semiestruturadas. Foram observadas 65 espécies pertencentes a 38 famílias botânicas. A família com maior número de espécies foi Lamiaceae e as espécies mais frequentes foram Carica papaya L. e Mangifera indica L. Alocasia macrorrhizos (L.) G. Don e Carica papaya L. foram as espécies mais abundantes. Dos entrevistados, 80% declararam utilizar plantas medicinais para tratamentos de doenças e 24 espécies têm uso medicinal. As categorias de uso predominantes foram alimentícia, medicinal e ornamental. Mentha X piperita L. e Lippia alba (Mill.) N. E. Br. ex Britton & P. Wilson foram as mais citadas como medicinal, Carica papaya e Mangifera indica como frutífera e Dieffenbachia picta Schott como ornamental.Ethnobotanical survey of plant species in neighborhood quintals in the city of Mossoró, Rio Grande do NorteAbstract: Homegardens are known by different denominations in various places and can be defined as a production area located near the house, where agricultural and forestry species are grown, with the creation of small domestic animals. The present work had as objective to carry out an ethnobotanical survey of the vegetal species existing in the homegardens of the Alto Sumaré neighborhood in Mossoró, Rio Grande do Norte. The selection of fifteen informants was done using the snowball technique and the data collection was done with direct observation and participant, guided tour, and semi-structured interviews. It was observed 65 species belonging to 38 botanical families. The family with the highest number of species was Lamiaceae and the most frequent species were Carica papaya L. and Mangifera indica L. Alocasia macrorrhizos (L.) G. Don and Carica papaya L. were the most abundant species. Of those interviewed, 80% reported using medicinal plants for disease treatments and 24 species have medicinal uses. The predominant categories of use were food, medicinal and ornamental. Mentha X piperita L. and Lippia alba (Mill.) N. E. Br. Ex Britton & P. Wilson were the most cited as medicinal, Carica papaya and Mangifera indica as fruitful and Dieffenbachia picta Schott as ornamental.
Youzhong Wan - One of the best experts on this subject based on the ideXlab platform.
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extracts and components of ficus Carica leaves suppress survival cell cycle and migration of triple negative breast cancer mda mb 231 cells
OncoTargets and Therapy, 2018Co-Authors: Yu Zhang, Youzhong Wan, Bo Huo, Yue JinAbstract:Background Products from Ficus Carica have been used in traditional medicine to treat many diseases. This study aimed to analyze anticancer effects of extracts of F. Carica leaves on the triple-negative breast cancer cell line MDA-MB-231. Materials and methods The human breast cancer cell line MDA-MB-231 was used to evaluate effects of F. Carica extracts. Effects of F. Carica on cell viability were evaluated using MTT assays. Cell-cycle distribution was examined using cell-cycle analysis. Wound-healing assays were used to evaluate migration of MDA-MB-231. Quantitative reverse-transcription polymerase chain reaction was used to detect levels of Bax, p53, p21, GATA3, ELF5, cyclin-dependent kinases, MMP2, and tissue inhibitors of metalloproteinase. Results We investigated the mechanism of anti-growth effects, and found that the expressions of genes that promote apoptosis were increased. In addition, the treated cells illustrated increased portion at S phase and changed expression of cyclin-dependent kinases, demonstrating cell-cycle arrest at the S phase. Furthermore, treated cells showed decreased cell mobility, which is essential for metastasis. Two of the active components of F. Carica leaves, bergapten and psoralen, had similar anticancer effects as F. Carica leaf extracts, indicating that these two components might play important roles in anticancer effects of F. Carica leaves. Conclusion Our findings suggest that F. Carica leaves might be a good source to develop drugs for suppressing cancer-cell growth and migration to treat triple-negative breast cancers.