The Experts below are selected from a list of 153 Experts worldwide ranked by ideXlab platform
G.e Robards - One of the best experts on this subject based on the ideXlab platform.
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Follicle characteristics, seasonal changes in fibre cross-sectional area and ellipticity in Australasian specialty Carpet Wool sheep, Romneys and Merinos.
Small ruminant research : the journal of the International Goat Association, 2000Co-Authors: Sc Champion, G.e RobardsAbstract:Data was collected on secondary:primary follicle ratio, relative follicle density, and seasonal changes in fibre cross-sectional area and fibre ellipticity (ratio of major-to-minor fibre cross-sectional axis) in the Australasian specialty Carpet Wool breeds (Carpetmaster, Drysdale, Elliottdale, Tukidale), Romneys and Merinos. Merinos had a higher secondary:primary follicle ratio (p
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follicle characteristics seasonal changes in fibre cross sectional area and ellipticity in australasian specialty Carpet Wool sheep romneys and merinos
Small Ruminant Research, 2000Co-Authors: Sc Champion, G.e RobardsAbstract:Data was collected on secondary:primary follicle ratio, relative follicle density, and seasonal changes in fibre cross-sectional area and fibre ellipticity (ratio of major-to-minor fibre cross-sectional axis) in the Australasian specialty Carpet Wool breeds (Carpetmaster, Drysdale, Elliottdale, Tukidale), Romneys and Merinos. Merinos had a higher secondary:primary follicle ratio (p<0.001), relative primary follicle density (p<0.05), relative secondary and relative total follicle density (p<0.01) than the other breeds. Both breed and follicle type (primary or secondary) had a significant effect on fibre cross-sectional area (p<0.05). While both fibre types (primary and secondary) showed changes in cross-sectional area during the experiment, a more distinct seasonal pattern was seen in the secondary fibres with summer maxima and winter minima in all breeds. Relative change in fibre diameter was higher in secondary fibres than in primary fibres. Changes in fibre ellipticity were also noted throughout the duration of the experiment. It is apparent that there are differences between primary and secondary follicle populations in the way the fibres produced contribute to seasonal changes in average fibre diameter of fleeces. The changes suggest there are separate control mechanisms for each follicle type and that the number, size and arrangement of the cells in each type of fibre vary independently of one another throughout the year, bringing about dynamic changes in fibre growth and form. Further work is required to examine the influence of feed quality on these factors and to determine the mechanisms through which these changes in fibre structure are brought about.
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EFFECTS OF SUPPLEMENTS ON Wool GROWTH RESPONSES OF MERINO, ROMNEY AND TUKIDALE SHEEP
1994Co-Authors: Sc Champion, G.e RobardsAbstract:SUMMARY During winter, the fleece production of Carpet Wool producing Tukidale ewes fed a grain-based ration was significantly greater than the growth by either Merinos (P c 0.01) or Romneys (P c 0.05). The response to the grain-based ration by Tukidales was also a oreater (P c 0.01) than their response to a protein-rich pellet ration. The Merinos consuming the pellet ration produced more Wool per unit area than those consuming the grain or chaff rations. The Romneys were intermediate in their overall level of fleece production and their response to both the grain-based and protein pellet rations.
Quanbang Pei - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome Reveals Long Non-coding RNAs and mRNAs Involved in Primary Wool Follicle Induction in Carpet Sheep Fetal Skin
Frontiers in physiology, 2018Co-Authors: Yangfan Nie, Xinting Zheng, Wenshuo Chen, Zhiwei Liu, Haisheng Qiao, Quanbang PeiAbstract:Murine primary hair follicle induction is driven by the communication between the mesenchyme and epithelium and mostly governed by signaling pathways including wingless-related integration site (WNT), ectodysplasin A receptor (EDAR), bone morphogenetic protein (BMP), and fibroblast growth factor (FGF), as observed in genetically modified mouse models. Sheep skin may serve as a valuable system for hair research owing to the co-existence of sweat glands with Wool follicles in trunk skin and asynchronized Wool follicle growth pattern similar to that of human head hair follicles. However, the mechanisms underlying Wool follicle development remain largely unknown. To understand how long non-coding RNAs (lncRNAs) and mRNAs function in primary Wool follicle induction in Carpet Wool sheep, we conducted high-throughput RNA sequencing and revealed globally altered lncRNAs (36 upregulated and 26 downregulated), mRNAs (228 elevated and 225 decreased), and 80 differentially expressed novel transcripts. Several key signals in WNT (WNT2B and WNT16), BMP (BMP3, BMP4, and BMP7), EDAR (EDAR and EDARADD), and FGF (FGFR2 and FGF20) pathways, and a series of lncRNAs, including XLOC_539599, XLOC_556463, XLOC_015081, XLOC_1285606, XLOC_297809, and XLOC_764219, were shown to be potentially important for primary Wool follicle induction. GO and KEGG analyses of differentially expressed mRNAs and potential targets of altered lncRNAs were both significantly enriched in morphogenesis biological processes and transforming growth factor-β, Hedgehog, and PI3K-Akt signaling, as well as focal adhesion and extracellular matrix-receptor interactions. The prediction of mRNA-mRNA and lncRNA-mRNA interaction networks further revealed transcripts potentially involved in primary Wool follicle induction. The expression patterns of mRNAs and lncRNAs of interest were validated by qRT-PCR. The localization of XLOC_297809 and XLOC_764219 both in placodes and dermal condensations was detected by in situ hybridization, indicating important roles of lncRNAs in primary Wool follicle induction and skin development. This is the first report elucidating the gene network of lncRNAs and mRNAs associated with primary Wool follicle early development in Carpet Wool sheep and will shed new light on selective Wool sheep breeding.
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Data_Sheet_1_Transcriptome Reveals Long Non-coding RNAs and mRNAs Involved in Primary Wool Follicle Induction in Carpet Sheep Fetal Skin.XLSX
2018Co-Authors: Yangfan Nie, Xinting Zheng, Wenshuo Chen, Zhiwei Liu, Haisheng Qiao, Quanbang PeiAbstract:Murine primary hair follicle induction is driven by the communication between the mesenchyme and epithelium and mostly governed by signaling pathways including wingless-related integration site (WNT), ectodysplasin A receptor (EDAR), bone morphogenetic protein (BMP), and fibroblast growth factor (FGF), as observed in genetically modified mouse models. Sheep skin may serve as a valuable system for hair research owing to the co-existence of sweat glands with Wool follicles in trunk skin and asynchronized Wool follicle growth pattern similar to that of human head hair follicles. However, the mechanisms underlying Wool follicle development remain largely unknown. To understand how long non-coding RNAs (lncRNAs) and mRNAs function in primary Wool follicle induction in Carpet Wool sheep, we conducted high-throughput RNA sequencing and revealed globally altered lncRNAs (36 upregulated and 26 downregulated), mRNAs (228 elevated and 225 decreased), and 80 differentially expressed novel transcripts. Several key signals in WNT (WNT2B and WNT16), BMP (BMP3, BMP4, and BMP7), EDAR (EDAR and EDARADD), and FGF (FGFR2 and FGF20) pathways, and a series of lncRNAs, including XLOC_539599, XLOC_556463, XLOC_015081, XLOC_1285606, XLOC_297809, and XLOC_764219, were shown to be potentially important for primary Wool follicle induction. GO and KEGG analyses of differentially expressed mRNAs and potential targets of altered lncRNAs were both significantly enriched in morphogenesis biological processes and transforming growth factor-β, Hedgehog, and PI3K-Akt signaling, as well as focal adhesion and extracellular matrix-receptor interactions. The prediction of mRNA-mRNA and lncRNA-mRNA interaction networks further revealed transcripts potentially involved in primary Wool follicle induction. The expression patterns of mRNAs and lncRNAs of interest were validated by qRT-PCR. The localization of XLOC_297809 and XLOC_764219 both in placodes and dermal condensations was detected by in situ hybridization, indicating important roles of lncRNAs in primary Wool follicle induction and skin development. This is the first report elucidating the gene network of lncRNAs and mRNAs associated with primary Wool follicle early development in Carpet Wool sheep and will shed new light on selective Wool sheep breeding.
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Image_1_Transcriptome Reveals Long Non-coding RNAs and mRNAs Involved in Primary Wool Follicle Induction in Carpet Sheep Fetal Skin.TIF
2018Co-Authors: Yangfan Nie, Xinting Zheng, Wenshuo Chen, Zhiwei Liu, Haisheng Qiao, Quanbang PeiAbstract:Murine primary hair follicle induction is driven by the communication between the mesenchyme and epithelium and mostly governed by signaling pathways including wingless-related integration site (WNT), ectodysplasin A receptor (EDAR), bone morphogenetic protein (BMP), and fibroblast growth factor (FGF), as observed in genetically modified mouse models. Sheep skin may serve as a valuable system for hair research owing to the co-existence of sweat glands with Wool follicles in trunk skin and asynchronized Wool follicle growth pattern similar to that of human head hair follicles. However, the mechanisms underlying Wool follicle development remain largely unknown. To understand how long non-coding RNAs (lncRNAs) and mRNAs function in primary Wool follicle induction in Carpet Wool sheep, we conducted high-throughput RNA sequencing and revealed globally altered lncRNAs (36 upregulated and 26 downregulated), mRNAs (228 elevated and 225 decreased), and 80 differentially expressed novel transcripts. Several key signals in WNT (WNT2B and WNT16), BMP (BMP3, BMP4, and BMP7), EDAR (EDAR and EDARADD), and FGF (FGFR2 and FGF20) pathways, and a series of lncRNAs, including XLOC_539599, XLOC_556463, XLOC_015081, XLOC_1285606, XLOC_297809, and XLOC_764219, were shown to be potentially important for primary Wool follicle induction. GO and KEGG analyses of differentially expressed mRNAs and potential targets of altered lncRNAs were both significantly enriched in morphogenesis biological processes and transforming growth factor-β, Hedgehog, and PI3K-Akt signaling, as well as focal adhesion and extracellular matrix-receptor interactions. The prediction of mRNA-mRNA and lncRNA-mRNA interaction networks further revealed transcripts potentially involved in primary Wool follicle induction. The expression patterns of mRNAs and lncRNAs of interest were validated by qRT-PCR. The localization of XLOC_297809 and XLOC_764219 both in placodes and dermal condensations was detected by in situ hybridization, indicating important roles of lncRNAs in primary Wool follicle induction and skin development. This is the first report elucidating the gene network of lncRNAs and mRNAs associated with primary Wool follicle early development in Carpet Wool sheep and will shed new light on selective Wool sheep breeding.
Sc Champion - One of the best experts on this subject based on the ideXlab platform.
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Follicle characteristics, seasonal changes in fibre cross-sectional area and ellipticity in Australasian specialty Carpet Wool sheep, Romneys and Merinos.
Small ruminant research : the journal of the International Goat Association, 2000Co-Authors: Sc Champion, G.e RobardsAbstract:Data was collected on secondary:primary follicle ratio, relative follicle density, and seasonal changes in fibre cross-sectional area and fibre ellipticity (ratio of major-to-minor fibre cross-sectional axis) in the Australasian specialty Carpet Wool breeds (Carpetmaster, Drysdale, Elliottdale, Tukidale), Romneys and Merinos. Merinos had a higher secondary:primary follicle ratio (p
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follicle characteristics seasonal changes in fibre cross sectional area and ellipticity in australasian specialty Carpet Wool sheep romneys and merinos
Small Ruminant Research, 2000Co-Authors: Sc Champion, G.e RobardsAbstract:Data was collected on secondary:primary follicle ratio, relative follicle density, and seasonal changes in fibre cross-sectional area and fibre ellipticity (ratio of major-to-minor fibre cross-sectional axis) in the Australasian specialty Carpet Wool breeds (Carpetmaster, Drysdale, Elliottdale, Tukidale), Romneys and Merinos. Merinos had a higher secondary:primary follicle ratio (p<0.001), relative primary follicle density (p<0.05), relative secondary and relative total follicle density (p<0.01) than the other breeds. Both breed and follicle type (primary or secondary) had a significant effect on fibre cross-sectional area (p<0.05). While both fibre types (primary and secondary) showed changes in cross-sectional area during the experiment, a more distinct seasonal pattern was seen in the secondary fibres with summer maxima and winter minima in all breeds. Relative change in fibre diameter was higher in secondary fibres than in primary fibres. Changes in fibre ellipticity were also noted throughout the duration of the experiment. It is apparent that there are differences between primary and secondary follicle populations in the way the fibres produced contribute to seasonal changes in average fibre diameter of fleeces. The changes suggest there are separate control mechanisms for each follicle type and that the number, size and arrangement of the cells in each type of fibre vary independently of one another throughout the year, bringing about dynamic changes in fibre growth and form. Further work is required to examine the influence of feed quality on these factors and to determine the mechanisms through which these changes in fibre structure are brought about.
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EFFECTS OF SUPPLEMENTS ON Wool GROWTH RESPONSES OF MERINO, ROMNEY AND TUKIDALE SHEEP
1994Co-Authors: Sc Champion, G.e RobardsAbstract:SUMMARY During winter, the fleece production of Carpet Wool producing Tukidale ewes fed a grain-based ration was significantly greater than the growth by either Merinos (P c 0.01) or Romneys (P c 0.05). The response to the grain-based ration by Tukidales was also a oreater (P c 0.01) than their response to a protein-rich pellet ration. The Merinos consuming the pellet ration produced more Wool per unit area than those consuming the grain or chaff rations. The Romneys were intermediate in their overall level of fleece production and their response to both the grain-based and protein pellet rations.
Yangfan Nie - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome Reveals Long Non-coding RNAs and mRNAs Involved in Primary Wool Follicle Induction in Carpet Sheep Fetal Skin
Frontiers in physiology, 2018Co-Authors: Yangfan Nie, Xinting Zheng, Wenshuo Chen, Zhiwei Liu, Haisheng Qiao, Quanbang PeiAbstract:Murine primary hair follicle induction is driven by the communication between the mesenchyme and epithelium and mostly governed by signaling pathways including wingless-related integration site (WNT), ectodysplasin A receptor (EDAR), bone morphogenetic protein (BMP), and fibroblast growth factor (FGF), as observed in genetically modified mouse models. Sheep skin may serve as a valuable system for hair research owing to the co-existence of sweat glands with Wool follicles in trunk skin and asynchronized Wool follicle growth pattern similar to that of human head hair follicles. However, the mechanisms underlying Wool follicle development remain largely unknown. To understand how long non-coding RNAs (lncRNAs) and mRNAs function in primary Wool follicle induction in Carpet Wool sheep, we conducted high-throughput RNA sequencing and revealed globally altered lncRNAs (36 upregulated and 26 downregulated), mRNAs (228 elevated and 225 decreased), and 80 differentially expressed novel transcripts. Several key signals in WNT (WNT2B and WNT16), BMP (BMP3, BMP4, and BMP7), EDAR (EDAR and EDARADD), and FGF (FGFR2 and FGF20) pathways, and a series of lncRNAs, including XLOC_539599, XLOC_556463, XLOC_015081, XLOC_1285606, XLOC_297809, and XLOC_764219, were shown to be potentially important for primary Wool follicle induction. GO and KEGG analyses of differentially expressed mRNAs and potential targets of altered lncRNAs were both significantly enriched in morphogenesis biological processes and transforming growth factor-β, Hedgehog, and PI3K-Akt signaling, as well as focal adhesion and extracellular matrix-receptor interactions. The prediction of mRNA-mRNA and lncRNA-mRNA interaction networks further revealed transcripts potentially involved in primary Wool follicle induction. The expression patterns of mRNAs and lncRNAs of interest were validated by qRT-PCR. The localization of XLOC_297809 and XLOC_764219 both in placodes and dermal condensations was detected by in situ hybridization, indicating important roles of lncRNAs in primary Wool follicle induction and skin development. This is the first report elucidating the gene network of lncRNAs and mRNAs associated with primary Wool follicle early development in Carpet Wool sheep and will shed new light on selective Wool sheep breeding.
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Data_Sheet_1_Transcriptome Reveals Long Non-coding RNAs and mRNAs Involved in Primary Wool Follicle Induction in Carpet Sheep Fetal Skin.XLSX
2018Co-Authors: Yangfan Nie, Xinting Zheng, Wenshuo Chen, Zhiwei Liu, Haisheng Qiao, Quanbang PeiAbstract:Murine primary hair follicle induction is driven by the communication between the mesenchyme and epithelium and mostly governed by signaling pathways including wingless-related integration site (WNT), ectodysplasin A receptor (EDAR), bone morphogenetic protein (BMP), and fibroblast growth factor (FGF), as observed in genetically modified mouse models. Sheep skin may serve as a valuable system for hair research owing to the co-existence of sweat glands with Wool follicles in trunk skin and asynchronized Wool follicle growth pattern similar to that of human head hair follicles. However, the mechanisms underlying Wool follicle development remain largely unknown. To understand how long non-coding RNAs (lncRNAs) and mRNAs function in primary Wool follicle induction in Carpet Wool sheep, we conducted high-throughput RNA sequencing and revealed globally altered lncRNAs (36 upregulated and 26 downregulated), mRNAs (228 elevated and 225 decreased), and 80 differentially expressed novel transcripts. Several key signals in WNT (WNT2B and WNT16), BMP (BMP3, BMP4, and BMP7), EDAR (EDAR and EDARADD), and FGF (FGFR2 and FGF20) pathways, and a series of lncRNAs, including XLOC_539599, XLOC_556463, XLOC_015081, XLOC_1285606, XLOC_297809, and XLOC_764219, were shown to be potentially important for primary Wool follicle induction. GO and KEGG analyses of differentially expressed mRNAs and potential targets of altered lncRNAs were both significantly enriched in morphogenesis biological processes and transforming growth factor-β, Hedgehog, and PI3K-Akt signaling, as well as focal adhesion and extracellular matrix-receptor interactions. The prediction of mRNA-mRNA and lncRNA-mRNA interaction networks further revealed transcripts potentially involved in primary Wool follicle induction. The expression patterns of mRNAs and lncRNAs of interest were validated by qRT-PCR. The localization of XLOC_297809 and XLOC_764219 both in placodes and dermal condensations was detected by in situ hybridization, indicating important roles of lncRNAs in primary Wool follicle induction and skin development. This is the first report elucidating the gene network of lncRNAs and mRNAs associated with primary Wool follicle early development in Carpet Wool sheep and will shed new light on selective Wool sheep breeding.
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Image_1_Transcriptome Reveals Long Non-coding RNAs and mRNAs Involved in Primary Wool Follicle Induction in Carpet Sheep Fetal Skin.TIF
2018Co-Authors: Yangfan Nie, Xinting Zheng, Wenshuo Chen, Zhiwei Liu, Haisheng Qiao, Quanbang PeiAbstract:Murine primary hair follicle induction is driven by the communication between the mesenchyme and epithelium and mostly governed by signaling pathways including wingless-related integration site (WNT), ectodysplasin A receptor (EDAR), bone morphogenetic protein (BMP), and fibroblast growth factor (FGF), as observed in genetically modified mouse models. Sheep skin may serve as a valuable system for hair research owing to the co-existence of sweat glands with Wool follicles in trunk skin and asynchronized Wool follicle growth pattern similar to that of human head hair follicles. However, the mechanisms underlying Wool follicle development remain largely unknown. To understand how long non-coding RNAs (lncRNAs) and mRNAs function in primary Wool follicle induction in Carpet Wool sheep, we conducted high-throughput RNA sequencing and revealed globally altered lncRNAs (36 upregulated and 26 downregulated), mRNAs (228 elevated and 225 decreased), and 80 differentially expressed novel transcripts. Several key signals in WNT (WNT2B and WNT16), BMP (BMP3, BMP4, and BMP7), EDAR (EDAR and EDARADD), and FGF (FGFR2 and FGF20) pathways, and a series of lncRNAs, including XLOC_539599, XLOC_556463, XLOC_015081, XLOC_1285606, XLOC_297809, and XLOC_764219, were shown to be potentially important for primary Wool follicle induction. GO and KEGG analyses of differentially expressed mRNAs and potential targets of altered lncRNAs were both significantly enriched in morphogenesis biological processes and transforming growth factor-β, Hedgehog, and PI3K-Akt signaling, as well as focal adhesion and extracellular matrix-receptor interactions. The prediction of mRNA-mRNA and lncRNA-mRNA interaction networks further revealed transcripts potentially involved in primary Wool follicle induction. The expression patterns of mRNAs and lncRNAs of interest were validated by qRT-PCR. The localization of XLOC_297809 and XLOC_764219 both in placodes and dermal condensations was detected by in situ hybridization, indicating important roles of lncRNAs in primary Wool follicle induction and skin development. This is the first report elucidating the gene network of lncRNAs and mRNAs associated with primary Wool follicle early development in Carpet Wool sheep and will shed new light on selective Wool sheep breeding.
Haisheng Qiao - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome Reveals Long Non-coding RNAs and mRNAs Involved in Primary Wool Follicle Induction in Carpet Sheep Fetal Skin
Frontiers in physiology, 2018Co-Authors: Yangfan Nie, Xinting Zheng, Wenshuo Chen, Zhiwei Liu, Haisheng Qiao, Quanbang PeiAbstract:Murine primary hair follicle induction is driven by the communication between the mesenchyme and epithelium and mostly governed by signaling pathways including wingless-related integration site (WNT), ectodysplasin A receptor (EDAR), bone morphogenetic protein (BMP), and fibroblast growth factor (FGF), as observed in genetically modified mouse models. Sheep skin may serve as a valuable system for hair research owing to the co-existence of sweat glands with Wool follicles in trunk skin and asynchronized Wool follicle growth pattern similar to that of human head hair follicles. However, the mechanisms underlying Wool follicle development remain largely unknown. To understand how long non-coding RNAs (lncRNAs) and mRNAs function in primary Wool follicle induction in Carpet Wool sheep, we conducted high-throughput RNA sequencing and revealed globally altered lncRNAs (36 upregulated and 26 downregulated), mRNAs (228 elevated and 225 decreased), and 80 differentially expressed novel transcripts. Several key signals in WNT (WNT2B and WNT16), BMP (BMP3, BMP4, and BMP7), EDAR (EDAR and EDARADD), and FGF (FGFR2 and FGF20) pathways, and a series of lncRNAs, including XLOC_539599, XLOC_556463, XLOC_015081, XLOC_1285606, XLOC_297809, and XLOC_764219, were shown to be potentially important for primary Wool follicle induction. GO and KEGG analyses of differentially expressed mRNAs and potential targets of altered lncRNAs were both significantly enriched in morphogenesis biological processes and transforming growth factor-β, Hedgehog, and PI3K-Akt signaling, as well as focal adhesion and extracellular matrix-receptor interactions. The prediction of mRNA-mRNA and lncRNA-mRNA interaction networks further revealed transcripts potentially involved in primary Wool follicle induction. The expression patterns of mRNAs and lncRNAs of interest were validated by qRT-PCR. The localization of XLOC_297809 and XLOC_764219 both in placodes and dermal condensations was detected by in situ hybridization, indicating important roles of lncRNAs in primary Wool follicle induction and skin development. This is the first report elucidating the gene network of lncRNAs and mRNAs associated with primary Wool follicle early development in Carpet Wool sheep and will shed new light on selective Wool sheep breeding.
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Data_Sheet_1_Transcriptome Reveals Long Non-coding RNAs and mRNAs Involved in Primary Wool Follicle Induction in Carpet Sheep Fetal Skin.XLSX
2018Co-Authors: Yangfan Nie, Xinting Zheng, Wenshuo Chen, Zhiwei Liu, Haisheng Qiao, Quanbang PeiAbstract:Murine primary hair follicle induction is driven by the communication between the mesenchyme and epithelium and mostly governed by signaling pathways including wingless-related integration site (WNT), ectodysplasin A receptor (EDAR), bone morphogenetic protein (BMP), and fibroblast growth factor (FGF), as observed in genetically modified mouse models. Sheep skin may serve as a valuable system for hair research owing to the co-existence of sweat glands with Wool follicles in trunk skin and asynchronized Wool follicle growth pattern similar to that of human head hair follicles. However, the mechanisms underlying Wool follicle development remain largely unknown. To understand how long non-coding RNAs (lncRNAs) and mRNAs function in primary Wool follicle induction in Carpet Wool sheep, we conducted high-throughput RNA sequencing and revealed globally altered lncRNAs (36 upregulated and 26 downregulated), mRNAs (228 elevated and 225 decreased), and 80 differentially expressed novel transcripts. Several key signals in WNT (WNT2B and WNT16), BMP (BMP3, BMP4, and BMP7), EDAR (EDAR and EDARADD), and FGF (FGFR2 and FGF20) pathways, and a series of lncRNAs, including XLOC_539599, XLOC_556463, XLOC_015081, XLOC_1285606, XLOC_297809, and XLOC_764219, were shown to be potentially important for primary Wool follicle induction. GO and KEGG analyses of differentially expressed mRNAs and potential targets of altered lncRNAs were both significantly enriched in morphogenesis biological processes and transforming growth factor-β, Hedgehog, and PI3K-Akt signaling, as well as focal adhesion and extracellular matrix-receptor interactions. The prediction of mRNA-mRNA and lncRNA-mRNA interaction networks further revealed transcripts potentially involved in primary Wool follicle induction. The expression patterns of mRNAs and lncRNAs of interest were validated by qRT-PCR. The localization of XLOC_297809 and XLOC_764219 both in placodes and dermal condensations was detected by in situ hybridization, indicating important roles of lncRNAs in primary Wool follicle induction and skin development. This is the first report elucidating the gene network of lncRNAs and mRNAs associated with primary Wool follicle early development in Carpet Wool sheep and will shed new light on selective Wool sheep breeding.
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Image_1_Transcriptome Reveals Long Non-coding RNAs and mRNAs Involved in Primary Wool Follicle Induction in Carpet Sheep Fetal Skin.TIF
2018Co-Authors: Yangfan Nie, Xinting Zheng, Wenshuo Chen, Zhiwei Liu, Haisheng Qiao, Quanbang PeiAbstract:Murine primary hair follicle induction is driven by the communication between the mesenchyme and epithelium and mostly governed by signaling pathways including wingless-related integration site (WNT), ectodysplasin A receptor (EDAR), bone morphogenetic protein (BMP), and fibroblast growth factor (FGF), as observed in genetically modified mouse models. Sheep skin may serve as a valuable system for hair research owing to the co-existence of sweat glands with Wool follicles in trunk skin and asynchronized Wool follicle growth pattern similar to that of human head hair follicles. However, the mechanisms underlying Wool follicle development remain largely unknown. To understand how long non-coding RNAs (lncRNAs) and mRNAs function in primary Wool follicle induction in Carpet Wool sheep, we conducted high-throughput RNA sequencing and revealed globally altered lncRNAs (36 upregulated and 26 downregulated), mRNAs (228 elevated and 225 decreased), and 80 differentially expressed novel transcripts. Several key signals in WNT (WNT2B and WNT16), BMP (BMP3, BMP4, and BMP7), EDAR (EDAR and EDARADD), and FGF (FGFR2 and FGF20) pathways, and a series of lncRNAs, including XLOC_539599, XLOC_556463, XLOC_015081, XLOC_1285606, XLOC_297809, and XLOC_764219, were shown to be potentially important for primary Wool follicle induction. GO and KEGG analyses of differentially expressed mRNAs and potential targets of altered lncRNAs were both significantly enriched in morphogenesis biological processes and transforming growth factor-β, Hedgehog, and PI3K-Akt signaling, as well as focal adhesion and extracellular matrix-receptor interactions. The prediction of mRNA-mRNA and lncRNA-mRNA interaction networks further revealed transcripts potentially involved in primary Wool follicle induction. The expression patterns of mRNAs and lncRNAs of interest were validated by qRT-PCR. The localization of XLOC_297809 and XLOC_764219 both in placodes and dermal condensations was detected by in situ hybridization, indicating important roles of lncRNAs in primary Wool follicle induction and skin development. This is the first report elucidating the gene network of lncRNAs and mRNAs associated with primary Wool follicle early development in Carpet Wool sheep and will shed new light on selective Wool sheep breeding.