The Experts below are selected from a list of 5988 Experts worldwide ranked by ideXlab platform
Lars Engebretsen - One of the best experts on this subject based on the ideXlab platform.
-
articular Cartilage lesions in 993 consecutive knee arthroscopies
American Journal of Sports Medicine, 2004Co-Authors: Asbjorn Aroen, Sverre Loken, Stig Heir, Elling Alvik, Arne Ekeland, Odd Granlund, Lars EngebretsenAbstract:BackgroundTraumatic articular Cartilage injuries heal poorly and may lead to development of osteoarthritis at a young age. This study estimates the number of patients who may benefit from one of the surgical methods of Cartilage repair.MethodsAll patients undergoing knee arthroscopy during a 6-month period at three collaborating hospitals were consecutively evaluated according to the International Cartilage Repair Society (ICRS) knee form. The material consists of 993 consecutive knee arthroscopies in patients with median age of 35 years.ResultsPreoperative radiographs demonstrated degenerative changes in 13% of the knees. Articular Cartilage Pathology was found in 66% and a localized Cartilage defect was found in 20% of the knees. A localized full-thickness Cartilage lesion (ICRS grade 3 and 4) was observed in 11% of the knees. Of the localized full-thickness lesions, 55% (6% of all knees) had a size above 2 cm2.ConclusionEleven percent of all knee arthroscopies show Cartilage defects that may be suitabl...
-
articular Cartilage lesions in 993 consecutive knee arthroscopies
American Journal of Sports Medicine, 2004Co-Authors: Asbjorn Aroen, Sverre Loken, Stig Heir, Elling Alvik, Arne Ekeland, Odd Granlund, Lars EngebretsenAbstract:BackgroundTraumatic articular Cartilage injuries heal poorly and may lead to development of osteoarthritis at a young age. This study estimates the number of patients who may benefit from one of the surgical methods of Cartilage repair.MethodsAll patients undergoing knee arthroscopy during a 6-month period at three collaborating hospitals were consecutively evaluated according to the International Cartilage Repair Society (ICRS) knee form. The material consists of 993 consecutive knee arthroscopies in patients with median age of 35 years.ResultsPreoperative radiographs demonstrated degenerative changes in 13% of the knees. Articular Cartilage Pathology was found in 66% and a localized Cartilage defect was found in 20% of the knees. A localized full-thickness Cartilage lesion (ICRS grade 3 and 4) was observed in 11% of the knees. Of the localized full-thickness lesions, 55% (6% of all knees) had a size above 2 cm2.ConclusionEleven percent of all knee arthroscopies show Cartilage defects that may be suitabl...
Laura B Creemers - One of the best experts on this subject based on the ideXlab platform.
-
cytokine profiles in the joint depend on Pathology but are different between synovial fluid Cartilage tissue and cultured chondrocytes
Arthritis Research & Therapy, 2014Co-Authors: A I Tsuchida, Daniel B F Saris, Wouter J A Dhert, M Beekhuizen, Marieke C T Hart, Timothy R D J Radstake, Gerjo J V M Van Osch, Laura B CreemersAbstract:This study aimed to evaluate whether profiles of several soluble mediators in synovial fluid and Cartilage tissue are Pathology-dependent and how their production is related to in vitro tissue formation by chondrocytes from diseased and healthy tissue. Samples were obtained from donors without joint Pathology (n = 39), with focal defects (n = 65) and osteoarthritis (n = 61). A multiplex bead assay (Luminex) was performed measuring up to 21 cytokines: Interleukin (IL)-1α, IL-1β, IL-1RA, IL-4, IL-6, IL-6Rα, IL-7, IL-8, IL-10, IL-13, tumor necrosis factor (TNF)α, Interferon (IFN)γ, oncostatin M (OSM), leukemia inhibitory factor (LIF), adiponectin, leptin, monocyte chemotactic factor (MCP)1, RANTES, basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), vascular growth factor (VEGF). In synovial fluid of patients with Cartilage Pathology, IL-6, IL-13, IFNγ and OSM levels were higher than in donors without joint Pathology (P ≤0.001). IL-13, IFNγ and OSM were also different between donors with Cartilage defects and OA (P <0.05). In Cartilage tissue from debrided defects, VEGF was higher than in non-pathological or osteoarthritic joints (P ≤0.001). IL-1α, IL-6, TNFα and OSM concentrations (in ng/ml) were markedly higher in Cartilage tissue than in synovial fluid (P <0.01). Culture of chondrocytes generally led to a massive induction of most cytokines (P <0.001). Although the release of inflammatory cytokines was also here dependent on the pathological condition (P <0.001) the actual profiles were different from tissue or synovial fluid and between non-expanded and expanded chondrocytes. Cartilage formation was lower by healthy unexpanded chondrocytes than by osteoarthritic or defect chondrocytes. Several pro-inflammatory, pro-angiogenic and pro-repair cytokines were elevated in joints with symptomatic Cartilage defects and/or osteoarthritis, although different cytokines were elevated in synovial fluid compared to tissue or cells. Hence a clear molecular profile was evident dependent on disease status of the joint, which however changed in composition depending on the biological sample analysed. These alterations did not affect in vitro tissue formation with these chondrocytes, as this was at least as effective or even better compared to healthy chondrocytes.
-
cytokine profiles in the joint depend on Pathology but are different between synovial fluid Cartilage tissue and cultured chondrocytes
Arthritis Research & Therapy, 2014Co-Authors: A I Tsuchida, Daniel B F Saris, Wouter J A Dhert, M Beekhuizen, Marieke C T Hart, Timothy R D J Radstake, Gerjo J V M Van Osch, Laura B CreemersAbstract:Introduction: This study aimed to evaluate whether profiles of several soluble mediators in synovial fluid and Cartilage tissue are Pathology-dependent and how their production is related to in vitro tissue formation by chondrocytes from diseased and healthy tissue. Methods: Samples were obtained from donors without joint Pathology (n = 39), with focal defects (n = 65) and osteoarthritis (n = 61). A multiplex bead assay (Luminex) was performed measuring up to 21 cytokines: Interleukin (IL)-1α, IL-1β, IL-1RA, IL-4, IL-6, IL-6Rα, IL-7, IL-8, IL-10, IL-13, tumor necrosis factor (TNF)α, Interferon (IFN)γ, oncostatin M (OSM), leukemia inhibitory factor (LIF), adiponectin, leptin, monocyte chemotactic factor (MCP)1, RANTES, basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), vascular growth factor (VEGF). Results: In synovial fluid of patients with Cartilage Pathology, IL-6, IL-13, IFNγ and OSM levels were higher than in donors without joint Pathology (P ≤0.001). IL-13, IFNγ and OSM were also different between donors with Cartilage defects and OA (P <0.05). In Cartilage tissue from debrided defects, VEGF was higher than in non-pathological or osteoarthritic joints (P ≤0.001). IL-1α, IL-6, TNFα and OSM concentrations (in ng/ml) were markedly higher in Cartilage tissue than in synovial fluid (P <0.01). Culture of chondrocytes generally led to a massive induction of most cytokines (P <0.001). Although the release of inflammatory cytokines was also here dependent on the pathological condition (P <0.001) the actual profiles were different from tissue or synovial fluid and between non-expanded and expanded chondrocytes. Cartilage formation was lower by healthy unexpanded chondrocytes than by osteoarthritic or defect chondrocytes. Conclusions: Several pro-inflammatory, pro-angiogenic and pro-repair cytokines were elevated in joints with symptomatic Cartilage defects and/or osteoarthritis, although different cytokines were elevated in synovial fluid compared to tissue or cells. Hence a clear molecular profile was evident dependent on disease status of the joint, which however changed in composition depending on the biological sample analysed. These alterations did not affect in vitro tissue formation with these chondrocytes, as this was at least as effective or even better compared to healthy chondrocytes.
-
joint injury and osteoarthritis soluble mediators in the course and treatment of Cartilage Pathology
Immunotherapy, 2009Co-Authors: Marijn Rutgers, Daniel B F Saris, Kiem Gie Auw Yang, Wouter J A Dhert, Laura B CreemersAbstract:Osteoarthritis is a disabling disease of the aging generation, which results in loss of quality of life and increased healthcare costs. Cytokines appear to play an important role in the cartilaginous degeneration characterizing the pathological process. Increasing experience is being gained with cytokine-modulating therapies aimed at interfering with effects of chondrodegradative cytokines in the synovial fluid. Although in vitro and in vivo effectiveness of several of these therapies has been demonstrated, clinical effectiveness remains disputable, which may be related to the low levels of inflammatory cytokines found in osteoarthritic joints. By contrast, directly after joint trauma, which has been shown to predipose to early osteoarthritis, synovial fluid cytokine levels are strongly increased. Cytokine-modulating therapies, however, have hardly been considered for this indication. Increased knowledge of intra-articular soluble mediators correlating with Cartilage Pathology will lead to further develop...
Fabrizio Manetti - One of the best experts on this subject based on the ideXlab platform.
-
smoothened antagonists reverse homogentisic acid induced alterations of hedgehog signaling and primary cilium length in alkaptonuria
Journal of Cellular Physiology, 2017Co-Authors: Silvia Gambassi, Giulia Bernardini, Daniela Braconi, Lia Millucci, Michela Geminiani, Stephen D Thorpe, Maurizio Orlandini, C L Thompson, Elena Petricci, Fabrizio ManettiAbstract:Alkaptonuria (AKU) is an ultra-rare genetic disease, in which the accumulation of a toxic metabolite, homogentisic acid (HGA) leads to the systemic development of ochronotic aggregates. These aggregates cause severe complications mainly at the level of joints with extensive degradation of the articular Cartilage. Primary cilia have been demonstrated to play an essential role in development and the maintenance of articular Cartilage homeostasis, through their involvement in mechanosignalling and Hedgehog signalling pathways. Hedgehog signalling has been demonstrated to be activated in osteoarthritis (OA) and to drive Cartilage degeneration in vivo. The numerous similarities between OA and AKU suggest that primary cilia hedgehog signalling may also be altered in AKU. Thus, we characterised an AKU cellular model in which healthy chondrocytes were treated with HGA (66 µM) to replicate AKU Cartilage Pathology. We investigated the degree of activation of the hedgehog signalling pathway and how treatment with inhibitors of the receptor Smoothened (Smo) influenced hedgehog activation and primary cilia structure. The results obtained in this work provide a further step in the comprehension of the pathophysiological features of AKU, suggesting a potential therapeutic approach to modulate AKU Cartilage degradation processes through manipulation of the hedgehog pathway. This article is protected by copyright. All rights reserved
Daniel B F Saris - One of the best experts on this subject based on the ideXlab platform.
-
cytokine profiles in the joint depend on Pathology but are different between synovial fluid Cartilage tissue and cultured chondrocytes
Arthritis Research & Therapy, 2014Co-Authors: A I Tsuchida, Daniel B F Saris, Wouter J A Dhert, M Beekhuizen, Marieke C T Hart, Timothy R D J Radstake, Gerjo J V M Van Osch, Laura B CreemersAbstract:This study aimed to evaluate whether profiles of several soluble mediators in synovial fluid and Cartilage tissue are Pathology-dependent and how their production is related to in vitro tissue formation by chondrocytes from diseased and healthy tissue. Samples were obtained from donors without joint Pathology (n = 39), with focal defects (n = 65) and osteoarthritis (n = 61). A multiplex bead assay (Luminex) was performed measuring up to 21 cytokines: Interleukin (IL)-1α, IL-1β, IL-1RA, IL-4, IL-6, IL-6Rα, IL-7, IL-8, IL-10, IL-13, tumor necrosis factor (TNF)α, Interferon (IFN)γ, oncostatin M (OSM), leukemia inhibitory factor (LIF), adiponectin, leptin, monocyte chemotactic factor (MCP)1, RANTES, basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), vascular growth factor (VEGF). In synovial fluid of patients with Cartilage Pathology, IL-6, IL-13, IFNγ and OSM levels were higher than in donors without joint Pathology (P ≤0.001). IL-13, IFNγ and OSM were also different between donors with Cartilage defects and OA (P <0.05). In Cartilage tissue from debrided defects, VEGF was higher than in non-pathological or osteoarthritic joints (P ≤0.001). IL-1α, IL-6, TNFα and OSM concentrations (in ng/ml) were markedly higher in Cartilage tissue than in synovial fluid (P <0.01). Culture of chondrocytes generally led to a massive induction of most cytokines (P <0.001). Although the release of inflammatory cytokines was also here dependent on the pathological condition (P <0.001) the actual profiles were different from tissue or synovial fluid and between non-expanded and expanded chondrocytes. Cartilage formation was lower by healthy unexpanded chondrocytes than by osteoarthritic or defect chondrocytes. Several pro-inflammatory, pro-angiogenic and pro-repair cytokines were elevated in joints with symptomatic Cartilage defects and/or osteoarthritis, although different cytokines were elevated in synovial fluid compared to tissue or cells. Hence a clear molecular profile was evident dependent on disease status of the joint, which however changed in composition depending on the biological sample analysed. These alterations did not affect in vitro tissue formation with these chondrocytes, as this was at least as effective or even better compared to healthy chondrocytes.
-
cytokine profiles in the joint depend on Pathology but are different between synovial fluid Cartilage tissue and cultured chondrocytes
Arthritis Research & Therapy, 2014Co-Authors: A I Tsuchida, Daniel B F Saris, Wouter J A Dhert, M Beekhuizen, Marieke C T Hart, Timothy R D J Radstake, Gerjo J V M Van Osch, Laura B CreemersAbstract:Introduction: This study aimed to evaluate whether profiles of several soluble mediators in synovial fluid and Cartilage tissue are Pathology-dependent and how their production is related to in vitro tissue formation by chondrocytes from diseased and healthy tissue. Methods: Samples were obtained from donors without joint Pathology (n = 39), with focal defects (n = 65) and osteoarthritis (n = 61). A multiplex bead assay (Luminex) was performed measuring up to 21 cytokines: Interleukin (IL)-1α, IL-1β, IL-1RA, IL-4, IL-6, IL-6Rα, IL-7, IL-8, IL-10, IL-13, tumor necrosis factor (TNF)α, Interferon (IFN)γ, oncostatin M (OSM), leukemia inhibitory factor (LIF), adiponectin, leptin, monocyte chemotactic factor (MCP)1, RANTES, basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), vascular growth factor (VEGF). Results: In synovial fluid of patients with Cartilage Pathology, IL-6, IL-13, IFNγ and OSM levels were higher than in donors without joint Pathology (P ≤0.001). IL-13, IFNγ and OSM were also different between donors with Cartilage defects and OA (P <0.05). In Cartilage tissue from debrided defects, VEGF was higher than in non-pathological or osteoarthritic joints (P ≤0.001). IL-1α, IL-6, TNFα and OSM concentrations (in ng/ml) were markedly higher in Cartilage tissue than in synovial fluid (P <0.01). Culture of chondrocytes generally led to a massive induction of most cytokines (P <0.001). Although the release of inflammatory cytokines was also here dependent on the pathological condition (P <0.001) the actual profiles were different from tissue or synovial fluid and between non-expanded and expanded chondrocytes. Cartilage formation was lower by healthy unexpanded chondrocytes than by osteoarthritic or defect chondrocytes. Conclusions: Several pro-inflammatory, pro-angiogenic and pro-repair cytokines were elevated in joints with symptomatic Cartilage defects and/or osteoarthritis, although different cytokines were elevated in synovial fluid compared to tissue or cells. Hence a clear molecular profile was evident dependent on disease status of the joint, which however changed in composition depending on the biological sample analysed. These alterations did not affect in vitro tissue formation with these chondrocytes, as this was at least as effective or even better compared to healthy chondrocytes.
-
joint injury and osteoarthritis soluble mediators in the course and treatment of Cartilage Pathology
Immunotherapy, 2009Co-Authors: Marijn Rutgers, Daniel B F Saris, Kiem Gie Auw Yang, Wouter J A Dhert, Laura B CreemersAbstract:Osteoarthritis is a disabling disease of the aging generation, which results in loss of quality of life and increased healthcare costs. Cytokines appear to play an important role in the cartilaginous degeneration characterizing the pathological process. Increasing experience is being gained with cytokine-modulating therapies aimed at interfering with effects of chondrodegradative cytokines in the synovial fluid. Although in vitro and in vivo effectiveness of several of these therapies has been demonstrated, clinical effectiveness remains disputable, which may be related to the low levels of inflammatory cytokines found in osteoarthritic joints. By contrast, directly after joint trauma, which has been shown to predipose to early osteoarthritis, synovial fluid cytokine levels are strongly increased. Cytokine-modulating therapies, however, have hardly been considered for this indication. Increased knowledge of intra-articular soluble mediators correlating with Cartilage Pathology will lead to further develop...
Asbjorn Aroen - One of the best experts on this subject based on the ideXlab platform.
-
articular Cartilage lesions in 993 consecutive knee arthroscopies
American Journal of Sports Medicine, 2004Co-Authors: Asbjorn Aroen, Sverre Loken, Stig Heir, Elling Alvik, Arne Ekeland, Odd Granlund, Lars EngebretsenAbstract:BackgroundTraumatic articular Cartilage injuries heal poorly and may lead to development of osteoarthritis at a young age. This study estimates the number of patients who may benefit from one of the surgical methods of Cartilage repair.MethodsAll patients undergoing knee arthroscopy during a 6-month period at three collaborating hospitals were consecutively evaluated according to the International Cartilage Repair Society (ICRS) knee form. The material consists of 993 consecutive knee arthroscopies in patients with median age of 35 years.ResultsPreoperative radiographs demonstrated degenerative changes in 13% of the knees. Articular Cartilage Pathology was found in 66% and a localized Cartilage defect was found in 20% of the knees. A localized full-thickness Cartilage lesion (ICRS grade 3 and 4) was observed in 11% of the knees. Of the localized full-thickness lesions, 55% (6% of all knees) had a size above 2 cm2.ConclusionEleven percent of all knee arthroscopies show Cartilage defects that may be suitabl...
-
articular Cartilage lesions in 993 consecutive knee arthroscopies
American Journal of Sports Medicine, 2004Co-Authors: Asbjorn Aroen, Sverre Loken, Stig Heir, Elling Alvik, Arne Ekeland, Odd Granlund, Lars EngebretsenAbstract:BackgroundTraumatic articular Cartilage injuries heal poorly and may lead to development of osteoarthritis at a young age. This study estimates the number of patients who may benefit from one of the surgical methods of Cartilage repair.MethodsAll patients undergoing knee arthroscopy during a 6-month period at three collaborating hospitals were consecutively evaluated according to the International Cartilage Repair Society (ICRS) knee form. The material consists of 993 consecutive knee arthroscopies in patients with median age of 35 years.ResultsPreoperative radiographs demonstrated degenerative changes in 13% of the knees. Articular Cartilage Pathology was found in 66% and a localized Cartilage defect was found in 20% of the knees. A localized full-thickness Cartilage lesion (ICRS grade 3 and 4) was observed in 11% of the knees. Of the localized full-thickness lesions, 55% (6% of all knees) had a size above 2 cm2.ConclusionEleven percent of all knee arthroscopies show Cartilage defects that may be suitabl...