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Antonio Orduña - One of the best experts on this subject based on the ideXlab platform.
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Serologic evidence of human infection by Francisella tularensis in the population of Castilla y León (Spain) prior to 1997.
FEMS immunology and medical microbiology, 2003Co-Authors: María P. Gutiérrez, Ana I. Dueñas, Miguel Ángel Bratos, Ana Almaraz, Rufino Alamo, Henar Rodríguez Marcos, José I. Garrote, María J Rodríguez Recio, Maria Fe Muñoz, Antonio OrduñaAbstract:Prior to an outbreak in Castilla y Leon in December 1997, tularaemia was practically non-existent in Spain. In this paper we studied the prevalence of antibodies against Francisella tularensis in a representative sample of the population (4825 people) from Castilla y Leon (Spain) in samples collected before this outbreak. Antibodies against F. tularensis were detected in nine (0.19%) of the 4825 sera, with antibody titres ranging from 1/20 to 1/160. Of these nine sera, one was positive in seroagglutination against Brucella. Seroagglutination against other bacteria (Yersinia enterocolitica O:9 and O:3 and Proteus OX19) was negative in all sera. Seroprevalence of antibodies in females was 0.20% and 0.17% in males; no statistically significant differences were found in prevalence in terms of sex, age or province.
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Prevalencia de anticuerpos frente a Francisella tularensis en la población de Castilla y León con anterioridad a 1997
Medicina clinica, 2003Co-Authors: M. Purificacion Gutierrez, Antonio Orduña, Ana I. Dueñas, Miguel Ángel Bratos, Ana Almaraz, Rufino Alamo, M. Jesús Rodríguez Recio, Henar Rodríguez Marcos, Antonio Rodríguez TorresAbstract:Fundamento y objetivos: Este trabajo pretende conocer la prevalencia de infeccion por Francisella tularensis en la poblacion de Castilla y Leon previa al brote de tularemia humana de finales de 1997. Sujetos y metodo: Se obtuvieron 4.825 sueros (entre abril de 1996 y abril de 1997) de residentes en Castilla y Leon. Se realizo una prueba de microaglutinacion en placa para detectar anticuerpos anti-F. tularensis. En los sueros positivos se llevaron a cabo seroaglutinaciones en tubo frente a Brucella, Yersinia enterocolitica y Proteus. Resultados: Se detectaron anticuerpos anti-F. tularensis en 9 (0,19%) de los 4.825 sueros. De esos 9 sueros, uno fue positivo en la seroaglutinacion frente a Brucella, siendo todos negativos frente a las otras bacterias. Conclusiones: Antes de 1997 la seroprevalencia de anticuerpos anti-F. tularensis en la poblacion de Castilla y Leon era baja (0,19%).
A Encinasgrandes - One of the best experts on this subject based on the ideXlab platform.
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spotted fever group rickettsiae in ticks feeding on humans in northwestern spain is rickettsia conorii vanishing
Annals of the New York Academy of Sciences, 2006Co-Authors: Pedro Fernandezsoto, Ricardo Perezsanchez, Rufino Alamosanz, A EncinasgrandesAbstract:: During a 7-year study, we identified and analyzed by PCR 4,049 ticks removed from 3,685 asymptomatic patients in Castilla y Leon (northwestern Spain). A total of 320 ticks (belonging to 10 species) were PCR-positive for rickettsiae. Comparison of amplicon sequences in databases enabled us to identify eight different spotted fever group (SFG) rickettsiae: Rickettsia slovaca, Rickettsia sp. IRS3/IRS4, R. massiliae/Bar29, R. aeschlimannii, Rickettsia sp. RpA4/DnS14, R. helvetica, Rickettsia sp. DmS1, and R. conorii. Although Mediterranean spotted fever (MSF) is an endemic disease in Castilla y Leon, R. conorii was found in only one Rhipicephalus sanguineus tick, whereas other pathogenic SFG rickettsiae were much more prevalent in the same area. Our data suggest that in Castilla y Leon, many MSF or MSF-like cases attributed to R. conorii could have been actually caused by other SFG rickettsiae present in ticks biting people in this region of Spain.
Pedro Fernandezsoto - One of the best experts on this subject based on the ideXlab platform.
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spotted fever group rickettsiae in ticks feeding on humans in northwestern spain is rickettsia conorii vanishing
Annals of the New York Academy of Sciences, 2006Co-Authors: Pedro Fernandezsoto, Ricardo Perezsanchez, Rufino Alamosanz, A EncinasgrandesAbstract:: During a 7-year study, we identified and analyzed by PCR 4,049 ticks removed from 3,685 asymptomatic patients in Castilla y Leon (northwestern Spain). A total of 320 ticks (belonging to 10 species) were PCR-positive for rickettsiae. Comparison of amplicon sequences in databases enabled us to identify eight different spotted fever group (SFG) rickettsiae: Rickettsia slovaca, Rickettsia sp. IRS3/IRS4, R. massiliae/Bar29, R. aeschlimannii, Rickettsia sp. RpA4/DnS14, R. helvetica, Rickettsia sp. DmS1, and R. conorii. Although Mediterranean spotted fever (MSF) is an endemic disease in Castilla y Leon, R. conorii was found in only one Rhipicephalus sanguineus tick, whereas other pathogenic SFG rickettsiae were much more prevalent in the same area. Our data suggest that in Castilla y Leon, many MSF or MSF-like cases attributed to R. conorii could have been actually caused by other SFG rickettsiae present in ticks biting people in this region of Spain.
María P. Gutiérrez - One of the best experts on this subject based on the ideXlab platform.
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Serologic evidence of human infection by Francisella tularensis in the population of Castilla y León (Spain) prior to 1997.
FEMS immunology and medical microbiology, 2003Co-Authors: María P. Gutiérrez, Ana I. Dueñas, Miguel Ángel Bratos, Ana Almaraz, Rufino Alamo, Henar Rodríguez Marcos, José I. Garrote, María J Rodríguez Recio, Maria Fe Muñoz, Antonio OrduñaAbstract:Prior to an outbreak in Castilla y Leon in December 1997, tularaemia was practically non-existent in Spain. In this paper we studied the prevalence of antibodies against Francisella tularensis in a representative sample of the population (4825 people) from Castilla y Leon (Spain) in samples collected before this outbreak. Antibodies against F. tularensis were detected in nine (0.19%) of the 4825 sera, with antibody titres ranging from 1/20 to 1/160. Of these nine sera, one was positive in seroagglutination against Brucella. Seroagglutination against other bacteria (Yersinia enterocolitica O:9 and O:3 and Proteus OX19) was negative in all sera. Seroprevalence of antibodies in females was 0.20% and 0.17% in males; no statistically significant differences were found in prevalence in terms of sex, age or province.
José Maria Tarjuelo - One of the best experts on this subject based on the ideXlab platform.
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Improving water management: The irrigation advisory service of Castilla-La Mancha (Spain)
Agricultural Water Management, 2005Co-Authors: J.f. Ortega, J.a. De Juan, José Maria TarjueloAbstract:This work presents the main methodological aspects and results obtained from the activity of the Irrigation Advisory Service for Farmers (SIAR) of Castilla-La Mancha, Spain. Castilla-La Mancha is a broad semi-arid region, with a total area of 8 million ha and an irrigable area of more an 475,000 ha. Most of these irrigated areas (at least 60%) present serious problems of water scarcity, and are in need of improvement in the use of irrigation water. SIAR upgrades farmers’ capacities and awareness, thus minimizing the negative environmental impacts of irrigation, decreasing the production costs and contributing to the sustainability of irrigated agriculture in the area. Approximately, 25% of the irrigated area is advised, representing 499 collaborator farmers within an area of ca. 100,000 ha. Water-scarce areas are where the SIAR advice is more closely followed by the farmers and irrigation performance indicators are highest. The total estimated cost for the service is of about €3 ha−1 yr−1. A team of field technicians forms the core of SIAR. The team is assembled via a cooperation agreement between the University of Castilla-La Mancha and the Regional Government. Among the results and conclusions obtained, the most important is the improvement of water use. However, technical, social and economic limitations are relevant, and constitute a handicap to achieve the objectives of the advisory service.
