The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform
Jerome Etienne - One of the best experts on this subject based on the ideXlab platform.
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new serotype of bartonella heriselae in endocarditis and cat scratch Disease
The Lancet, 1996Co-Authors: Michel Drancourt, Richard J Birtles, G Chaumentin, François Vandenesch, Didier Raoult, Jerome EtienneAbstract:Abstract Summary Background The fastidious nature of Bartonella henselae is such that demonstration of clinical infection relies mainly on serological or molecular biological methods. Only five isolates have been obtained from patients with Cat-Scratch Disease (CSD) and none from endocarditis. Methods We isolated B henselae from a CSD patient and, for the first time, from a patient with endocarditis. The isolates were characterised on the basis of morphology, biochemistry, cell-wall fatty-acid analysis, PCR-restriction fragment length polymorphism analysis of the 16-23S intragenic spacer region, and 16S rRNA gene sequences. Characterisation of these isolates indicated them to belong to a new serogroup, which we have called "Marseille", and to a new genotype based on the 16S rRNA gene sequence. The new variant was incorporated into an immunofluorescence antibody test (IFAT), which was used to reassess serum samples from 113 CSD patients who were seronegative with the conventional IFAT. Findings 18 (16%) of these apparently seronegative patients yielded significantly raised titres. 20 CSD patients who were seropositive as judged by the conventional IFAT remained seropositive with the new IFAT. Interpretation Antigenic variability within the species is one possible reason for inconsistent results in the serological diagnosis of CSD.
Michel Drancourt - One of the best experts on this subject based on the ideXlab platform.
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new serotype of bartonella heriselae in endocarditis and cat scratch Disease
The Lancet, 1996Co-Authors: Michel Drancourt, Richard J Birtles, G Chaumentin, François Vandenesch, Didier Raoult, Jerome EtienneAbstract:Abstract Summary Background The fastidious nature of Bartonella henselae is such that demonstration of clinical infection relies mainly on serological or molecular biological methods. Only five isolates have been obtained from patients with Cat-Scratch Disease (CSD) and none from endocarditis. Methods We isolated B henselae from a CSD patient and, for the first time, from a patient with endocarditis. The isolates were characterised on the basis of morphology, biochemistry, cell-wall fatty-acid analysis, PCR-restriction fragment length polymorphism analysis of the 16-23S intragenic spacer region, and 16S rRNA gene sequences. Characterisation of these isolates indicated them to belong to a new serogroup, which we have called "Marseille", and to a new genotype based on the 16S rRNA gene sequence. The new variant was incorporated into an immunofluorescence antibody test (IFAT), which was used to reassess serum samples from 113 CSD patients who were seronegative with the conventional IFAT. Findings 18 (16%) of these apparently seronegative patients yielded significantly raised titres. 20 CSD patients who were seropositive as judged by the conventional IFAT remained seropositive with the new IFAT. Interpretation Antigenic variability within the species is one possible reason for inconsistent results in the serological diagnosis of CSD.
Francois Eb - One of the best experts on this subject based on the ideXlab platform.
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lymph node biopsy specimens and diagnosis of cat scratch Disease
Emerging Infectious Diseases, 2006Co-Authors: Jeanmarc Rolain, Hubert Lepidi, Michel Zanaret, Jeanmichel Triglia, Gerard Michel, P Thomas, Michele Texereau, Andreas Stein, Anette Romaru, Francois EbAbstract:We report microbiologic analysis of 786 lymph node biopsy specimens from patients with suspected Cat-Scratch Disease (CSD). The specimens were examined by standard, cell culture, and molecular methods. Infectious agents were found in samples from 391 (49.7%) of 786 patients. The most commonly identified infectious agent was Bartonella henselae (245 patients, 31.2%), the agent of CSD. Mycobacteriosis was diagnosed in 54 patients (6.9%) by culture and retrospectively confirmed by using a specific real-time PCR assay. Neoplasm was diagnosed in 181 specimens suitable for histologic analysis (26.0%) from 47 patients. Moreover, 13 patients with confirmed Bartonella infections had concurrent mycobacteriosis (10 cases) or neoplasm (3 cases). A diagnosis of CSD does not eliminate a diagnosis of mycobacteriosis or neoplasm. Histologic analysis of lymph node biopsy specimens should be routinely performed because some patients might have a concurrent malignant Disease or mycobacteriosis.
M. P. G. Koopmans - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of an in-house cat scratch Disease IgM ELISA to detect Bartonella henselae in a routine laboratory setting
European Journal of Clinical Microbiology & Infectious Diseases, 2009Co-Authors: M. Herremans, J. Bakker, M. J. Vermeulen, J. F. P. Schellekens, M. P. G. KoopmansAbstract:Cat scratch Disease (CSD) is caused by Bartonella henselae infection and is a common cause of regional lymphadenopathy. The diagnosis of CSD largely depends on serology, but detection of B. henselae in an affected lymph node by PCR is also an important diagnostic tool. We evaluated an IgM in-house ELISA protocol and analyzed its performance in routine CSD serology. Serum samples from PCR-positive patients ( n = 126), PCR-negative patients ( n = 123), and age-matched controls ( n = 126) were used for evaluation. The sensitivity of the IgM ELISA was only 56%, showing that the performance of B. henselae serology under routine laboratory settings is low, probably caused by the wide variability in Disease duration in patients suspected of CSD whose samples were submitted to our laboratory. Most patients (46%) with a positive IgM response were between 0 and 20 years of age. We conclude that the serodiagnosis of B. henselae is hampered by the low sensitivity and specificity of the assays when used in a routine laboratory setting. For this reason, a negative IgM or PCR result can never exclude CSD, especially with late sample collection.
Richard J Birtles - One of the best experts on this subject based on the ideXlab platform.
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new serotype of bartonella heriselae in endocarditis and cat scratch Disease
The Lancet, 1996Co-Authors: Michel Drancourt, Richard J Birtles, G Chaumentin, François Vandenesch, Didier Raoult, Jerome EtienneAbstract:Abstract Summary Background The fastidious nature of Bartonella henselae is such that demonstration of clinical infection relies mainly on serological or molecular biological methods. Only five isolates have been obtained from patients with Cat-Scratch Disease (CSD) and none from endocarditis. Methods We isolated B henselae from a CSD patient and, for the first time, from a patient with endocarditis. The isolates were characterised on the basis of morphology, biochemistry, cell-wall fatty-acid analysis, PCR-restriction fragment length polymorphism analysis of the 16-23S intragenic spacer region, and 16S rRNA gene sequences. Characterisation of these isolates indicated them to belong to a new serogroup, which we have called "Marseille", and to a new genotype based on the 16S rRNA gene sequence. The new variant was incorporated into an immunofluorescence antibody test (IFAT), which was used to reassess serum samples from 113 CSD patients who were seronegative with the conventional IFAT. Findings 18 (16%) of these apparently seronegative patients yielded significantly raised titres. 20 CSD patients who were seropositive as judged by the conventional IFAT remained seropositive with the new IFAT. Interpretation Antigenic variability within the species is one possible reason for inconsistent results in the serological diagnosis of CSD.