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Iyarajan - One of the best experts on this subject based on the ideXlab platform.
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Influence of Brassinosteroids (BRs) on the vincristine content of Catharanthus roseus (L). G. Don
European Journal of Experimental Biology, 2015Co-Authors: S. Muthulakshmi, IyarajanAbstract:Catharanthus roseus (L). G. Don is an important anticancerous plant belonging to the family Apocynaceae. It contains so many alkaloids like Vinblastine and vincristine are mainly present in aerial parts of C. roseus, which are used in treatment of various human cancers, so it is considered as mile stone in cancer chemotherapy. Vincristine is also used to cure cancer including acute leukemia, Hodgkin's and non- Hodgkin's lymphoma, neuroblastoma, rhabdomyosarcoma, Ewing's sarcoma, Wilms' tumor, multiple myeloma, chronic leukemias, thyroid cancer, brain tumors and blood disorders. In our study the effect of BRs on vincristine content of Catharanthus roseus was quantified. BRswas applied as foliar spray at different concentrations viz 5, 10, 15, and 20 ppm for 30days regularly. The vincristine content was quantified by HPLC method. The vincristine content was found to increase at all the concentration when compared to the control. The maximum concentration of vincristine was found to be around 15 and 20 ppm of BRs. Hence it is concluded that regular application of BRs to Catharanthus roseus can be recommended for attaining better biomass production and vincristine content.
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Influence of IAA on the vincristine content of Catharanthus roseus (L). G. Don.
Asian Journal of Plant Science & Research, 2013Co-Authors: S. Muthulakshmi, IyarajanAbstract:Catharanthus roseus (L). G. Don is an important anticancerous plant belonging to the family Apocyanaceae. It contains so many alkaloids like vincristine, vinblastine and ajmalcine which are pharmacologically important. This plant may be considered as a famous chemical factory for biosynthesis of a huge array of alkaloids and many of these chemicals are utilized as medicines. The effect of IAA on vincristine content of Catharanthus roseus was studied. IAA was applied as foliar spray at different concentrations viz 50, 100, 150, and 200ppm for 30days. Vincristine was quantified by HPLC method. Vincristine was significantly maximum at 150 and 200ppm concentration of IAA when compared to other concentrations and control. Hence it is concluded that practice of application of IAA to Catharanthus roseus can be recommended for attaining better biomass production and vincristine content.
Qifang Pan - One of the best experts on this subject based on the ideXlab platform.
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Overexpression of ORCA3 and G10H in Catharanthus roseus Plants Regulated Alkaloid Biosynthesis and Metabolism Revealed by NMR-Metabolomics
2016Co-Authors: Qifang Pan, Quan Wang, Fang Yuan, Shihai Xing, Yuesheng Tian, Jingya Zhao, Young Hae Choi, Robert Verpoorte, Guofeng Wang, Kexuan TangAbstract:In order to improve the production of the anticancer dimeric indole alkaloids in Catharanthuse roseus, much research has been dedicated to culturing cell lines, hairy roots, and efforts to elucidate the regulation of the monoterpenoid indole alkaloid (MIA) biosynthesis. In this study, the ORCA3 (Octadecanoid-derivative Responsive Catharanthus AP2-domain) gene alone or integrated with the G10H (geraniol 10-hydroxylase) gene were first introduced into C. roseus plants. Transgenic C. roseus plants overexpressing ORCA3 alone (OR lines), or co-overexpressing G10H and ORCA3 (GO lines) were obtained by genetic modification. ORCA3 overexpression induced an increase of AS, TDC, STR and D4H transcripts but did not affect CRMYC2 and G10H transcription. G10H transcripts showed a significant increase under G10H and ORCA3 co-overexpression. ORCA3 and G10H overexpression significantly increased the accumulation of strictosidine, vindoline, catharanthine and ajmalicine but had limited effects on anhydrovinblastine and vinblastine levels. NMR-based metabolomics confirmed the higher accumulation of monomeric indole alkaloids in OR and GO lines. Multivariate data analysis of 1H NMR spectra showed change of amino acid, organic acid, sugar and phenylpropanoid levels in both OR and GO lines compared to the controls. The result indicated that enhancement of MIA biosynthesis by ORCA3 and G10H overexpression might affect othe
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overexpression of orca3 and g10h in Catharanthus roseus plants regulated alkaloid biosynthesis and metabolism revealed by nmr metabolomics
PLOS ONE, 2012Co-Authors: Qifang Pan, Quan Wang, Fang Yuan, Shihai Xing, Yuesheng Tian, Jingya Zhao, Young Hae Choi, Robert Verpoorte, Guofeng WangAbstract:In order to improve the production of the anticancer dimeric indole alkaloids in Catharanthuse roseus, much research has been dedicated to culturing cell lines, hairy roots, and efforts to elucidate the regulation of the monoterpenoid indole alkaloid (MIA) biosynthesis. In this study, the ORCA3 (Octadecanoid-derivative Responsive Catharanthus AP2-domain) gene alone or integrated with the G10H (geraniol 10-hydroxylase) gene were first introduced into C. roseus plants. Transgenic C. roseus plants overexpressing ORCA3 alone (OR lines), or co-overexpressing G10H and ORCA3 (GO lines) were obtained by genetic modification. ORCA3 overexpression induced an increase of AS, TDC, STR and D4H transcripts but did not affect CRMYC2 and G10H transcription. G10H transcripts showed a significant increase under G10H and ORCA3 co-overexpression. ORCA3 and G10H overexpression significantly increased the accumulation of strictosidine, vindoline, catharanthine and ajmalicine but had limited effects on anhydrovinblastine and vinblastine levels. NMR-based metabolomics confirmed the higher accumulation of monomeric indole alkaloids in OR and GO lines. Multivariate data analysis of 1H NMR spectra showed change of amino acid, organic acid, sugar and phenylpropanoid levels in both OR and GO lines compared to the controls. The result indicated that enhancement of MIA biosynthesis by ORCA3 and G10H overexpression might affect other metabolic pathways in the plant metabolism of C. roseus.
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Effect of plant growth regulators on the biosynthesis of vinblastine, vindoline and catharanthine in Catharanthus roseus
Plant Growth Regulation, 2009Co-Authors: Qifang Pan, Xiaofen Sun, Yu Chen, Quan Wang, Fang Yuan, Shihai Xing, Yuesheng Tian, Jingya Zhao, Kexuan TangAbstract:Catharanthuse roseus is a well-known medicinal plant for its two valuable anticancer compounds: vinblastine and vincristine, which belongs to terpenoid indole alkaloids. Great efforts have been made to study the principles of its secondary metabolic pathways to regulate the alkaloids biosynthesis. In this article, different plant growth regulators were shortly applied to Catharanthus roseus plants during the blooming period to study their effects on the biosynthesis of vinblastine, vindoline and catharanthine. Salicylic acid and ethylene (ethephon) treatments resulted in a significant increase of vinblastine, vindoline and catharanthine while abscisic acid and gibberellic acid had a strongly negative influence on the accumulation of the three important alkaloids. Methyl jasmonate showed no great effect on the production of these valuable alkaloids. Chlormequat chloride highly enhanced the accumulation of vinblastine but greatly decreased the contents of vindoline and catharanthine.
S. Bindu - One of the best experts on this subject based on the ideXlab platform.
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Melatonin improves bioreductant capacity and silver nanoparticles synthesis using Catharanthus roseus leaves
RSC Advances, 2015Co-Authors: S. A. Sheshadri, S. Sriram, P. Balamurugan, R. Anupriya, S. Adline Princy, P. Brindha, S. BinduAbstract:Melatonin improved antioxidant potential, therapeutic metabolites and silver nanoparticles (with potent activity against urinary tract pathogens) in Catharanthus roseus leaves.
S. Muthulakshmi - One of the best experts on this subject based on the ideXlab platform.
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Influence of Brassinosteroids (BRs) on the vincristine content of Catharanthus roseus (L). G. Don
European Journal of Experimental Biology, 2015Co-Authors: S. Muthulakshmi, IyarajanAbstract:Catharanthus roseus (L). G. Don is an important anticancerous plant belonging to the family Apocynaceae. It contains so many alkaloids like Vinblastine and vincristine are mainly present in aerial parts of C. roseus, which are used in treatment of various human cancers, so it is considered as mile stone in cancer chemotherapy. Vincristine is also used to cure cancer including acute leukemia, Hodgkin's and non- Hodgkin's lymphoma, neuroblastoma, rhabdomyosarcoma, Ewing's sarcoma, Wilms' tumor, multiple myeloma, chronic leukemias, thyroid cancer, brain tumors and blood disorders. In our study the effect of BRs on vincristine content of Catharanthus roseus was quantified. BRswas applied as foliar spray at different concentrations viz 5, 10, 15, and 20 ppm for 30days regularly. The vincristine content was quantified by HPLC method. The vincristine content was found to increase at all the concentration when compared to the control. The maximum concentration of vincristine was found to be around 15 and 20 ppm of BRs. Hence it is concluded that regular application of BRs to Catharanthus roseus can be recommended for attaining better biomass production and vincristine content.
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Influence of IAA on the vincristine content of Catharanthus roseus (L). G. Don.
Asian Journal of Plant Science & Research, 2013Co-Authors: S. Muthulakshmi, IyarajanAbstract:Catharanthus roseus (L). G. Don is an important anticancerous plant belonging to the family Apocyanaceae. It contains so many alkaloids like vincristine, vinblastine and ajmalcine which are pharmacologically important. This plant may be considered as a famous chemical factory for biosynthesis of a huge array of alkaloids and many of these chemicals are utilized as medicines. The effect of IAA on vincristine content of Catharanthus roseus was studied. IAA was applied as foliar spray at different concentrations viz 50, 100, 150, and 200ppm for 30days. Vincristine was quantified by HPLC method. Vincristine was significantly maximum at 150 and 200ppm concentration of IAA when compared to other concentrations and control. Hence it is concluded that practice of application of IAA to Catharanthus roseus can be recommended for attaining better biomass production and vincristine content.
Rob J. Aerts - One of the best experts on this subject based on the ideXlab platform.
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Signalling molecules and the synthesis of alkaloids in Catharanthus roseus seedlings
Phytochemistry, 1996Co-Authors: Rob J. Aerts, Andrea Schäfer, Manfred Hesse, Thomas W. Baumann, Alan J. SlusarenkoAbstract:The Madagascar periwinkle, Catharanthus roseus, produces numerous alkaloids, several of which have important pharmaceutical uses. Catharanthus seedlings rapidly accumulate the monomeric alkaloids, vindoline, catharanthine and tabersonine, during germination. Various plant signalling molecules were tested for their ability to enhance alkaloid synthesis in Catharanthus seedlings. The compounds tested included plant hormones, fatty acid-derived messengers and agents that can induce systemic-acquired resistance in plants. Of these compounds, only methyl jasmonate (MeJa) enhanced the synthesis of monomeric alkaloids. However, feeding of a MeJa biosynthetic precursor, or inhibition of the lipoxygenase pathway, had no effect on alkaloid production in the seedlings. We conclude that, although MeJa is able to enhance alkaloid synthesis when supplied exogenously, the lipoxygenase pathway probably does not play a role in the regulation of alkaloid synthesis during normal germination of Catharanthus. Furthermore, it was found that Catharanthus seedlings accumulate small quantities of the dimeric alkaloid, vinblastine, a valuable antitumour drug. Contrary to its effect on the accumulation of monomeric alkaloids, MeJa did not influence the accumulation of vinblastine.
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Phytochrome is involved in the light-regulation of vindoline biosynthesis in Catharanthus.
Plant physiology, 1992Co-Authors: Rob J. Aerts, Vincenzo De LucaAbstract:The enzyme acetylcoenzyme A:deacetylvindoline 4-O-acetyl-transferase (DAT) catalyzes the final step in the biosynthesis of the monoterpenoid indole alkaloid, vindoline. Previous studies have shown that the appearance of DAT activity in etiolated seedlings of Catharanthus roseus is induced by exposure of seedlings to light and that enzyme activity is restricted principally to the cotyledons. Evidence is now presented that phytochrome is involved in the light-mediated induction of DAT activity in Catharanthus cotyledons.